Figure 1

PfCHA rescues yeast Saccharomyces cerevisiae Ca²⁺/H⁺antiporter mutant. A. Apoaequorin bioluminescence of cytosolic Ca²⁺ following exposure to extracellular Ca²⁺. Light emission was initially recorded for 8 seconds in low Ca2+ medium. CaCl₂ was subsequently added to 50 mM final concentration in 2 ml total volume. The luminescence recorded for 90 seconds is shown. BY4741n is the haploid isogenic yeast parental strain, vcx1 Δ + p505 is the vcx1 Δ knock-out carrying the pGREG505 plasmid only, vcx1 Δ + VCX1 and vcx1 Δ + PfCHA are the same knock-out strain but carrying recombinant plasmids with the homologueous VCX1 gene or the Plasmodium falciparum PfCHA gene, respectively. B. Apoaequorin luminescence measurements following a challenge with 500 mM extracellular CaCl2. Addition of CaCl2 is indicated by the arrows. Data represent means and standard deviations of three experiments (n=3).