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Figure 5 | Malaria Journal

Figure 5

From: A yeast expression system for functional and pharmacological studies of the malaria parasite Ca2+/H+ antiporter

Figure 5

PfCHA protein localizes to the yeast vacuole membrane. A. Indirect immunofluorescence assay with Anti-PfCHA in yeast vcx1Δ expressing the recombinants pGREG505-VCX1 and pGREG505-PfCHA. Bright field, dark field, and the merge in columns from left to right. Top panels show a sample from cells carrying pGREG505-VCX1 and stained with propidium iodide for staining of the nucleus in addition to the primary Anti-PfCHA. Bottom panels shown FITC fluorescence localizing around the yeast tonoplast of vcx1Δ cells carrying pGREG505-PfCHA and probed with Anti-PfCHA (1:100). B. Anti-PfCAX Western Blot. Top panel shows the signal given by Anti-PfCHA on the yeast membrane extracts. The expected molecular size for PfCHA is approximately 49kDa. Samples are labelled as the yeast vcx1Δ mutant carrying the empty pGREG505 vector (p505), or the recombinant pGREG-VCX1 or pGREG-PfCHA vectors. Bottom panel show the control with Anti-actin antibody. Molecular mass markers as indicated.

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Malaria Journal

ISSN: 1475-2875

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