Figure 5
From: Anti-plasmodial action of de novo-designed, cationic, lysine-branched, amphipathic, helical peptides
ΔFd-mediated parasite egressed from red blood cells are not coated with host cell membrane. Bright field optical images (top panel) show haemozoin crystals that are intracellular in control and appear to be extracellular in ΔFd (12.5 μM)-treated sample. Panel 2 shows DAPI stained nuclei of the malaria parasite. Panel 3 (immunostaining with band 3 antibody) indicates that band 3 (red) was seen in all cells. Panel 4 (overlay of DAPI and band 3) indicates that the parasites (staining blue) in control panel are intracellular and flanked by band 3 stain. But the ΔFd-treated parasites, which egressed anomalously, are extracellular and not flanked by band 3.