Recognition of Plasmodium falciparum gametocyte surface antigens by plasma antibodies in asymptomatic Ghanaian school children
Malaria Journal volume 11, Article number: P24 (2012)
Malaria transmission-reducing interventions are key components of malaria control and elimination . However, little is known about the immune responses directed at circulating Plasmodium falciparum gametocytes in humans, knowledge of which would be useful in the development of anti-gametocyte vaccines, which would have the capability to reduce malaria transmission from humans to mosquitoes. In a study in the Gambia, mature gametocyte-infected erythrocytes of P. falciparum were found to carry antigens (gametocyte surface antigens, GSA) that were recognised by malaria patient’s plasma antibodies. These anti-GSA antibodies, taken at a single timepoint, were weakly associated with lower duration of gametocyte carriage in these treated patients [2, 3]. We then sought to determine longitudinal patterns in GSA antibody prevalence and its relationship to possible immune suppression of gametocyte carriage in vivo.
Materials and methods
Flow cytometry of cultured gametocyte-infected erythrocytes from 3D7 and from two recently adapted gametocyte-producung lines was used to detect and measure plasma antibodies recognising the erythrocyte surface. Plasma was obtained from asymptomatic P./falciparum-positive children attending school in a rainforest region in Ghana. These children were treated with dihydro-artemisinin piperaquine, and followed up weekly for 1 month.
Results and conclusions
By microscopy, 8.9% (15/168) of the children enrolled carried gametocytes and a further 20% of them developed gametocytes during subsequent follow-up. (NASBA is also now being carried out to identify sub-microscopic gametocyte carriers.) Preliminary results from 113 samples tested in flow cytometry show that more than 50% of those in the sub-group of children with gametocytes at enrolment carry antibodies to GSA, and we expect this proportion to increase as gametocytes are developed during the follow-up. Further longitudinal flow cytometry, and NASBA analyses will enable us to understand the dynamics between immune responses to gametocytes and gametocyte carriage following treatment of asymptomatic malaria.
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Dinko, B., Bousema, T. & Sutherland, C.J. Recognition of Plasmodium falciparum gametocyte surface antigens by plasma antibodies in asymptomatic Ghanaian school children. Malar J 11 (Suppl 1), P24 (2012). https://doi.org/10.1186/1475-2875-11-S1-P24