Submicroscopic Plasmodium falciparum malaria and low birth weight in an area of unstable malaria transmission in Central Sudan
© Mohammed et al.; licensee BioMed Central Ltd. 2013
Received: 8 February 2013
Accepted: 24 May 2013
Published: 28 May 2013
Malaria, which frequently occurs in pregnant women in the tropics, is a leading cause of maternal anaemia and low birth weight (LBW) in infants. Few data exist concerning malaria infections that are present at submicroscopic levels during pregnancy and their LBW delivery in babies.
A case–control study (87 in each group) was conducted at the Medani Hospital, Central Sudan. Cases were women who had LBW deliveries where the infants weighed < 2,500 g. Controls were parturient women without having LBW babies. Obstetrical and medical characteristics were gathered from both groups through structured questionnaires. Both cases and controls were investigated for malaria using microscopic blood film analysis, placental histology and polymerase chain reaction (PCR). Microscopic and PCR analyses were conducted on maternal peripheral blood, placenta, and umbilical cord samples. Infant weights were recorded immediately after birth.
Plasmodium falciparum-positive blood films were not obtained from any of the women (cases or controls). Twenty-seven (31.0%) versus 22 (25.3%) (P = 0.500) of the cases and controls, respectively, had placental malaria infections as determined by histological examination. In comparison to the controls, the submicroscopic malaria infection prevalence rates were significantly higher in the cases; 24 (27.6%) vs six (7.0%), P < 0.001. Multivariate analysis showed that while malaria infection of the placenta (based on histology) was not associated with LBW, submicroscopic P. falciparum infection (OR = 6.89, 95% CI = 2.2–20.8; P = 0.001), or a combination of histologically determined and submicroscopic infections (OR = 2.45, 95% CI = 1.2–4.9; P = 0.012), were significantly associated with LBW.
In Central Sudan, pregnant women were at a higher risk of having an LBW delivery if they had submicroscopic infections rather than a histological diagnosis of placental malaria.
Malaria is a big public health problem in tropical countries, especially sub-Saharan Africa. Around 125 million pregnant women live in malaria-endemic areas and 32 million of these are at risk of malaria in sub-Saharan Africa[1, 2]. Malaria during pregnancy can lead to maternal anaemia and low birth weight (LBW) delivery, the latter of which is the main risk for neonatal and infant morbidity and mortality[3–5]. Malaria during pregnancy is caused by parasites sequestering in the placenta where selection of pregnancy-associated Plasmodium falciparum erythrocyte membrane protein-1 (Pf EMP-1) variant surface antigen occurs. Thus, placental malaria infection (especially in areas of unstable malaria transmission) may be detected in the absence of peripheral blood parasitaemia[7, 8]. Sequestration of malaria parasites in the placenta may lead to functional damage of placental villi, disturb the foetomaternal compartment and lead to LBW[9, 10].
Although placental histology is the ‘gold standard’ for malaria diagnosis during pregnancy, it is often not available in most settings where malaria is endemic such as sub-Saharan Africa. Polymerase chain reaction (PCR) is an alternative diagnostic tool that is widely used to diagnose malaria infection during pregnancy[12, 13]. However, there are few published studies, as well as inconsistent findings on associations between submicroscopic malaria infections and LBW[14–16].
In Sudan, malaria during pregnancy is a big health problem because women are more susceptible to malaria (peripheral, placental and submicroscopic infections) during pregnancy regardless of their age or parity and severe cases of malaria have been observed[7, 12, 17–19]. Malaria has many adverse effects on pregnancy and its outcome and it is a leading cause of maternal and perinatal mortality in Sudan[20–22].
The aim of this study was to build upon the previous work on placental malaria and LBW in Sudan[23–25]; specifically, to investigate the effect of submicroscopic levels of malaria parasites during pregnancy on birth weight. The study took place in the Medani Maternity Hospital in Central Sudan.
A case–control study was conducted during the post rainy season (September to November) 2010 at the labour ward of the Medani Maternity Hospital, Central Sudan. Central Sudan is characterized by unstable malaria transmission and P. falciparum is the sole malaria parasite species in the area and the transmission during the rainy (July –September) and post-rainy season. Medani Maternity Hospital is a tertiary hospital for women who receive antenatal care at the hospital or are referred from other clinics and hospitals, and women who live close to the hospital facility. Women with high-risk pregnancies are referred to the hospital. However, the referral criteria are not strictly adhered to and many women without a high-risk pregnancy deliver at the hospital.
A total sample size was calculated to provide 80% power to detect the difference of 5% at α = 0.05 and assumed 10% of women would not respond or have incomplete data. In this study, a case represents a woman who had an LBW delivery (<2,500 g). A consecutive woman who delivered next to the case was taken as control for each case. Controls were parturient women who have no LBW delivery (≥ 2,500 g). Women pregnant with twins and those with hypertension, diabetes mellitus or antepartum haemorrhage were excluded from the study in both case or controls groups. After obtaining a signed informed consent, women in the case and control groups were enlisted to participate in the study. Information on socio-demographics, obstetrics history, medical characteristics and antennal attendance were gathered through structured pretested questionnaires. Women in both groups were asked if they used bed nets and if they had experienced malaria infections in the index pregnancy. Body mass index was calculated by measuring maternal weight and height, which was expressed as weight (kg)/height (m)2. Babies were weighed immediately following birth to the nearest 10 g on a Salter scale. Scales were checked for accuracy on a weekly basis. The gender of each baby was recorded.
Giemsa-stained blood smears and light microscopy
Peripheral blood films were prepared from the mother, along with placental and umbilical cord samples. Both thick and thin blood films were prepared and stained with10% Giemsa and the parasite counts were obtained by counting the number of asexual parasites per 200 leukocytes assuming a leukocyte count of 8,000 leukocytes/μl (for thick films) or per 1,000 red blood cells (for thin films); blood films were considered negative if no parasites were detected in 100 oil immersion fields of a thick blood film.
Full thickness placental blocks around 3 cm were taken from the placenta and kept in neutral buffered formalin for histopathological examination. Buffer was used to prevent formalin pigment formation, which has similar optical characteristics and polarized light activity as malaria pigment. Placental malaria infections were characterized as previously described by Bulmer et al.: uninfected (no parasites or pigment), acute (parasites in intervillous spaces), chronic (parasites in maternal erythrocytes and pigment in fibrin, or cells within fibrin and/or chorionic villous syncytiotrophoblast or stroma), and past (no parasites and pigment confined to fibrin or cells within fibrin). The slide was read by a pathologist (AAM) who remained blind about the clinical characteristics and the arms of the study.
Parasite DNA extraction and PCR
Plasmodium falciparum DNA extraction and PCR assays were performed as described in the recent work[12, 24]. In brief, three drops of blood were collected onto a piece of filter paper from maternal peripheral blood, the maternal side of the placenta, and the umbilical cord. These samples were air-dried and stored at ambient temperature in individual sterile plastic bags. The specimens were transported for processing and analysis in the lab in Khartoum. Approximately 25 μl (corresponding to approximately one third of a spot) of blood was punched out from the dried blood spots. The filter paper piece was washed with distilled water and placed directly in a PCR reaction tube containing 25 μl of all the PCR reaction components. A negative control sample with no template DNA and an internal positive control were used for quality control purposes. Genomic DNA was checked, in an assay based on a nested PCR, for DNA from P. falciparum. PCR assays were performed by two of the team (HMI and MIE) who were both blinded to the clinical and the histology study data.
The malarial infection status of a participant was defined as any sample positive by microscopic and submicroscopic analysis or placental histology. Microscopic P. falciparum infections where determined at delivery from peripheral blood, placenta, and umbilical cord blood smears. Submicroscopic infections were defined as those participants with negative thick blood smears, but who were positive for P. falciparum based on the PCR results for peripheral blood, placenta, and umbilical cord samples. The P. falciparum negative group was defined by the absence of P. falciparum in thick blood smears, placental histology and PCR of peripheral blood, placental and umbilical cord samples.
The study received ethical clearance from the Research Board at the Faculty of Medicine, University of Khartoum, Sudan.
Data were analysed using SPSS for Windows version 16.0. Data means and proportions were compared by Student’s-test, X2 and Fisher’s exact tests as appropriate. Univariate and multivariate analyses were performed using LBW as the dependent variable. Maternal socio-demographic characteristics (age, parity, education, residence, antenatal care), malaria infection status (diagnosed by histology), and submicroscopic blood analysis (maternal peripheral blood, placenta and cord, or a combination) were included as possible influencing factors. P < 0.05 was regarded as significant.
Socio-demographic characteristics and malaria status in the cases and controls
Number (%) of
Low birth weight (N = 87)
Controls (N = 87)
Antenatal care ≤ thee time
Educational level < secondary
Bed net coverage
Anaemia (haemoglobin < 11 g/dl)
Malaria status in the cases and controls
Number (%) of
Low birth weight (N = 87)
Controls (N = 87)
All malaria infection (histology/submicroscopic
Placental malaria (histology)
Maternal, placental and cord
In comparison to the controls, the prevalence of submicroscopic malaria infection was highly significant in the cases; 24 (27.6%) vs six (7.0%); P < 0.001. None (0%), 19 (21.8%) and six (7.0%) vs four (4.6%), two (2.3%) and none (0%) were maternal, placental or cord submicroscopic malaria infections in the two study groups, respectively. One case had a placental and umbilical cord submicroscopic malaria infection, Table 2.
Significantly higher numbers of the cases than the controls had malaria infections (placental malaria infections on histology/submicroscopic malaria infection); 46 (53.0%) vs 26 (30.0%), P = 0.002. Out of these malaria infections, six (five and one in the cases and controls, respectively) had histologically positive placental malaria infections as well as submicroscopic malaria infections (Figure 1).
Effects of malaria infection on birth weight
Malaria infection as a risk factor for low birth weight infants
Factors associated with low birth weight in Medani Maternity Hospital, Central Sudan based on univariate or multivariate analysis
Educational level < secondary
Lack of antenatal care
Body mass index
Placental malaria infections (histology)
Submicroscopic malaria infections
All malaria infections
The main findings of the current study were as follows. While there was no significant difference in the prevalence of placental malaria by histological examination (31.0% vs 25.3; P = 0.500) between the two groups, significantly higher numbers of the cases had submicroscopic malaria infections than the controls (27.6% vs 7.0%; P < 0.001). While placental malaria infections that were positive by histology were not associated with LBW, submicroscopic malaria infections were, and this was a statistically significant finding. In fact, the impetus for this study arose out of the knowledge that two cross-sectional studies in Eastern Sudan failed to show significant associations between LBW delivery and placental malaria infection, as diagnosed by histology[7, 18]. The prevalence (28.0%) of histologically determined placental malaria infections in both groups (cases and controls) in the current study is similar to the prevalence of the placental malaria infections recently observed in Eastern and Central Sudan[7, 18, 23].
Interestingly, in the current study only six of the malaria infections (28.0%) had both placental malaria infections (histology) and submicroscopic malaria infections. The performance of PCR versus histology for diagnosing placental malaria infections was recently investigated in the same hospital (Medani Hospital, Central Sudan). The low sensitivity and specificity was explained by the different nature of the infections detected by the different methods (histology and PCR).
In the current study, while women with placental malaria infections (confirmed by histology) were not at risk of having an LBW delivery, women who had submicroscopic malaria infections had a seven-fold higher risk of such an event. In addition, birth weights were significantly lower in women who had submicroscopic malaria infections. Interestingly, it has been shown that pregnant women in Burkina Faso with submicroscopic malaria infections delivered infants that weighed significantly less than those delivered by women with no placental infections. Yet in the same study, delivery of LBW babies was not more common among women with submicroscopic placental malaria parasitaemia than those women without malaria. In Gabon, it has been found that women with submicroscopic P. falciparum infections had a 13-fold higher risk of LBW delivery compared with non-infected pregnant women. Remarkably, malaria infections in pregnant Kenyan women as estimated by real-time quantitative PCR were strongly associated with LBW delivery, but malaria detected by nested PCR showed a weaker association.
Conversely, the current study identified an association between submicroscopic malaria and LBW; this contrasts with previous reports from Malawi[16, 31] and Ghana, where no statistically significant association between submicroscopic P. falciparum infection and LBW was observed.
One of the limitations of the current study was the inability, by its design, to investigate the other effects of submicroscopic P. falciparum malaria on pregnancy outcomes such as anaemia. This is because it was designed to investigate LBW through a case–control study; another study is needed if the effects of submicroscopic P. falciparum malaria on haemoglobin are to be investigated. The second limitation is the sample size, where the current study failed to yield enough maternal, placental and umbilical cord submicroscopic P. falciparum malaria infections to enable parasite genotyping to be conducted. Therefore, a larger cross-sectional study is needed to address the effect on haemoglobin and parasite genotyping.
In Central Sudan, pregnant women were at higher risk of having an LBW delivery if they had submicroscopic infections rather than a diagnosis of placental malaria based on histology.
We wish to thank all of the patients for their cooperation in this study. We are very grateful to the local health authority in Geizera State and to the entire staff of Medani Maternity Hospital for their assistance. This work was funded by the National Fund for the promotion of Medical Service, Khartoum, Sudan.
- WHO: World malaria report 2010. 2010, Geneva, Switzerland: World Health Organization, Available: http://www.who.int/malaria/world_malaria_report_2010/worldmalariareport2010pdGoogle Scholar
- Dellicour S, Tatem AJ, Guerra CA, Snow RW, ter Kuile FO: Quantifying the number of pregnancies at risk of malaria in 2007: A demographic study. PLoS Med. 2010, 7: e1000221-10.1371/journal.pmed.1000221.PubMed CentralView ArticlePubMedGoogle Scholar
- Menendez C: Malaria during pregnancy: a priority area of malaria research and control. Parasitol Today. 1995, 11: 178-183. 10.1016/0169-4758(95)80151-0.View ArticlePubMedGoogle Scholar
- Steketee RW, Nahlen BL, Parise ME, Menendez C: The burden of malaria in pregnancy in malaria-endemic areas. Am J Trop Med Hyg. 2001, 64: 28-35.PubMedGoogle Scholar
- Menendez C, D’Alessandro U, ter Kuile FO: Reducing the burden of malaria in pregnancy by preventive strategies. Lancet Infect Dis. 2007, 7: 126-135. 10.1016/S1473-3099(07)70024-5.View ArticlePubMedGoogle Scholar
- Hviid L: The role of Plasmodium falciparum variant surface antigens in protective immunity and vaccine development. Hum Vaccin. 2010, 6: 84-89. 10.4161/hv.6.1.9602.View ArticlePubMedGoogle Scholar
- Adam I, Adam GK, Mohmmed AA, Salih MM, Ibrahuim SA, Ryan CA: Placental malaria and lack of prenatal care in an area of unstable malaria transmission in eastern Sudan. J Parasitol. 2009, 5: 751-752.View ArticleGoogle Scholar
- Sartelet H, Milko-Sartelet I, Garraud O, Picot S: Plasmodium falciparum persists in the placenta after three days’ treatment with quinine. Trans R Soc Trop Med Hyg. 1997, 91: 431-10.1016/S0035-9203(97)90269-8.View ArticlePubMedGoogle Scholar
- Menendez C, Ordi J, Ismail MR, Ventura PJ, Aponte JJ, Kahigwa E, Font F, Alonso PL: The impact of placental malaria on gestational age and birth weight. J Infect Dis. 2000, 181: 1740-1745. 10.1086/315449.View ArticlePubMedGoogle Scholar
- Ordi J, Ismail MR, Ventura PJ, Kahigwa E, Hirt R, Cardesa A, Alonso PL, Menendez C: Massive chronic intervillositis of the placenta associated with malaria infection. Am J Surg Pathol. 1998, 22: 1006-1011. 10.1097/00000478-199808000-00011.View ArticlePubMedGoogle Scholar
- Rogerson SJ, Mkundika P, Kanjala MK: Diagnosis of Plasmodium falciparum malaria at delivery: comparison of blood film preparation methods and of blood films with histology. J Clin Microbiol. 2003, 41: 1370-1374. 10.1128/JCM.41.4.1370-1374.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Adam I, A-Elbasit IE, Salih I, Elbashir MI: Submicroscopic Plasmodium falciparum infections during pregnancy, in an area of Sudan with a low intensity of malaria transmission. Ann Trop Med Parasitol. 2005, 99: 339-344. 10.1179/136485905X36244.View ArticlePubMedGoogle Scholar
- Kattenberg JH, Ochodo EA, Boer KR, Schallig HD, Mens PF, Leeflang MM: Systematic review and meta-analysis: rapid diagnostic tests versus placental histology, microscopy and PCR for malaria in pregnant women. Malar J. 2011, 10: 321-10.1186/1475-2875-10-321.PubMed CentralView ArticlePubMedGoogle Scholar
- Singer LM, Newman RD, Diarra A, Moran AC, Huber CS, Stennies G, Sirima SB, Konate A, Yameogo M, Sawadogo R, Barnwell JW, Parise ME: Evaluation of a malaria rapid diagnostic test for assessing the burden of malaria during pregnancy. Am J Trop Med Hyg. 2004, 70: 481-485.PubMedGoogle Scholar
- Adegnika AA, Verweij JJ, Agnandji ST, Chai SK, Breitling LP, Ramharter M, Frolich M, Issifou S, Kremsner PG, Yazdanbakhsh M: Microscopic and sub-microscopic Plasmodium falciparum infection, but not inflammation caused by infection, is associated with low birth weight. Am J Trop Med Hyg. 2006, 75: 798-803.PubMedGoogle Scholar
- Mankhambo L, Kanjala M, Rudman S, Lema VM, Rogerson SJ: Evaluation of the OptiMAL rapid antigen test and species- specific PCR to detect placental Plasmodium falciparum infection at delivery. J Clin Microbiol. 2002, 40: 155-158. 10.1128/JCM.40.1.155-158.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Adam I, Khamis AH, Elbashir MI: Prevalence and risk factors for malaria in pregnant women of eastern Sudan. Malar J. 2005, 4: 8-10.1186/1475-2875-4-8.View ArticleGoogle Scholar
- Adam I, Babiker S, Mohmmed AA, Salih MM, Prins MH, Zaki ZM: ABO blood group system and placental malaria in an area of unstable malaria transmission in eastern Sudan. Malar J. 2007, 6: 110-10.1186/1475-2875-6-110.PubMed CentralView ArticlePubMedGoogle Scholar
- Ali AA, Elhassan EM, Magzoub MM, Elbashir MI, Adam I: Hypoglycaemia and severe Plasmodium falciparum malaria among pregnant Sudanese women in an area characterized by unstable malaria transmission. Parasit Vectors. 2011, 4: 88-10.1186/1756-3305-4-88.PubMed CentralView ArticlePubMedGoogle Scholar
- Adam I, Elhassan EM, Haggaz AE, Ali AA, Adam GK: A perspective of the epidemiology of malaria and anaemia and their impact on maternal and perinatal outcomes in Sudan. J Infect Dev Ctries. 2011, 8: 3-7.Google Scholar
- Elhassan EM, Mirghani OA, Adam I: High maternal mortality and stillbirth in the Wad Medani Hospital, Central Sudan, 2003–2007. Trop Doct. 2009, 39: 238-239. 10.1258/td.2009.090005.View ArticlePubMedGoogle Scholar
- Bader E, Alhaj AM, Hussan AA, Adam I: Malaria and stillbirth in Omdurman Maternity Hospital, Sudan. Int J Gynaecol Obstet. 2010, 109: 144-146. 10.1016/j.ijgo.2009.11.022.View ArticlePubMedGoogle Scholar
- Adam I, Ehassan EM, Mohmmed AA, Salih MM, Elbashir MI:Decreased susceptibility to placental malaria in anaemic women in an area with unstable malaria transmission in central Sudan. Pathog Glob Health. 2012, 106: 118-121. 10.1179/2047773212Y.0000000011.PubMed CentralView ArticlePubMedGoogle Scholar
- Elbashir HM, Salih MM, Elhassan EM, Mohmmed AA, Elbashir MI, Adam I: Polymerase chain reaction and histology in diagnosis of placental malaria in an area of unstable malaria transmission in Central Sudan. Diagn Pathol. 2011, 6: 128-10.1186/1746-1596-6-128.PubMed CentralView ArticlePubMedGoogle Scholar
- Elhassan EM, Abbaker AO, Haggaz AD, Abubaker MS, Adam I: Anaemia and low birth weight in Medani Hospital Sudan. BMC Res Notes. 2010, 3: 181-10.1186/1756-0500-3-181.PubMed CentralView ArticlePubMedGoogle Scholar
- Malik EM, Atta HY, Weis M, Lang A, Puta C, Lettenmaier C: Sudan Roll Back Malaria Consultative Mission: Essential Actions to Support the Attainment of the Abuja Targets. Sudan RBM Country Consultative Mission Final Report. 2004, Geneva: Roll Back Malaria PartnershipGoogle Scholar
- Bulmer JN, Rasheed FN, Francis N, Morrison L, Greenwood BM: Placental malaria I. Pathological classification. Histopathology. 1993, 22: 211-218. 10.1111/j.1365-2559.1993.tb00110.x.View ArticlePubMedGoogle Scholar
- Bulmer JN, Rasheed FN, Morrison L, Francis N, Greenwood BM: Placental malaria II. A semi-quantitative investigation of the pathological features. Histopathology. 1993, 22: 219-225. 10.1111/j.1365-2559.1993.tb00111.x.View ArticlePubMedGoogle Scholar
- Snounou G, Viriyakosol S, Xhu XP, Jarra W, Pinhero L, do Rosario VE, Thaithong S, Brown KN: High sensitivity of detection of human malaria parasites by use of nested polymerase chain reaction. Mol Biochem Parasitol. 1993, 61: 315-320. 10.1016/0166-6851(93)90077-B.View ArticlePubMedGoogle Scholar
- Malhotra I, Dent A, Mungai P, Muchiri E, King CL: Real-time quantitative PCR for determining the burden of Plasmodium falciparum parasites during pregnancy and infancy. J Clin Microbiol. 2005, 43: 3630-3635. 10.1128/JCM.43.8.3630-3635.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Rantala AM, Taylor SM, Trottman PA, Luntamo M, Mbewe B, Maleta K, Kulmala T, Ashorn P, Meshnick SR: Comparison of real-time PCR and microscopy for malaria parasite detection in Malawian pregnant women. Malar J. 2010, 9: 26-10.1186/1475-2875-9-26.View ArticleGoogle Scholar
- Mockenhaupt FP, Bedu-Addo G, von Gaertner C, Boyé R, Fricke K, Hannibal I, Karakaya F, Schaller M, Ulmen U, Acquah PA, Dietz E, Eggelte TA, Bienzle U: Detection and clinical manifestation of placental malaria in southern Ghana. Malar J. 2006, 5: 119-10.1186/1475-2875-5-119.PubMed CentralView ArticlePubMedGoogle Scholar
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