- Open Access
Exploration of in vivo efficacy of artemether-lumefantrine against uncomplicated Plasmodium falciparum malaria in under fives in Tabora region, Tanzania
© Joseph et al.; licensee BioMed Central Ltd. 2013
- Received: 9 November 2012
- Accepted: 5 February 2013
- Published: 11 February 2013
Tanzania adopted artemether-lumefantrine (AL) as first-line drug for uncomplicated malaria in 2006. Recently, there was an anecdotal report on high malaria recurrence rate following AL treatment in in the (urban and peri-urban), western part of Tanzania. The current report is an exploratory study to carefully and systematically assess AL efficacy in the area.
Between June and August 2011, a total of 1,126 patients were screened for malaria, 33 had malaria, of which 20 patients met inclusion criteria and were enrolled and treated with standard dose of AL as recommended in the WHO protocol. Treated patients were followed up for 28 days to assess treatment responses. Before treatment (Day 0) and post-treatment (Day 7) plasma lumefantrine levels were determined to assess prior AL use and ascertain parasites exposure to adequate plasma leveles of lumefantrine, respectively.
The cure rate was 100%. All Day 0 plasma lumefantrine were below HPLC detectable level. The median Day 7 lumefantrine concentration was 404, (range, 189–894 ng/ml). Six out of 20 patients (30%) were gametocytaemic and all cleared gametocytes by Day 14. One patient showed an increase in gametocytes from four on Day 0 to 68, per 500 WBC on Day 2.
Artemether lumefantrine is highly efficacious against uncomplicated Plasmodium falciparum malaria. The elevation of gametocytaemia despite AL treatment needs to be evaluated in a larger study.
- Artemisinin Resistance
- Parasitological Response
The adoption of artemisinin-based combination therapy (ACT) in sub-Saharan Africa as first-line drug for the treatment of uncomplicated malaria  was largely based on experience gained in low transmission areas of Southeast Asia using artesunate-mefloquine (AS-MQ) . The few studies conducted in sub-Saharan Africa prior to adoption of AS+AQ and AL recorded nearly 100% cure rate [3, 4]. However, the long-term impact and extended useful therapeutic life of these drug combinations in high transmission areas remains unclear. Already increased parasite clearance half-life and reduced susceptibility to artemisinins and artesunate-mefloquine has been reported in Thai-Cambodia and Thai-Myanmar boarder [5, 6]. Indeed there is now both phenotypic  and genetic  evidence of artemisinin resistance in these areas. It is hypothesized that if not controlled, artemisinin resistance might eventually spread all over the malaria-endemic world. Thus WHO recommends increased monitoring and surveillance to evaluate the threat of artemisinin resistance and identify new foci rapidly and to provide information for immediate and comprehensive containment and prevention activities. Therefore, this exploratory study was carried out as an action plan in response to reports of high recurrent rate following AL treatment in Tabora, western Tanzania (pers comm, Dr Ruth Hulser, St Phillip’s Health Centre, Tabora) in order to rule out possibility of AL resistance in the region.
Study site, design, sampling frame and sample size
An in vivo study was carried out between mid-June and mid-August 2011 at Kitete Regional Hospital and St. Philip’s and St Anne’s health facilities serving the urban and peri-urban areas of Tabora municipality. Tabora is a high-burden area and malaria transmission peaks during the rain season, i.e., February to May . Children aged six to 59 months reporting to the hospital with fever or history of fever underwent physical and clinical examination by the study clinician. Inclusion and exclusion criteria are as described in the WHO protocol for high transmission areas . The recruited patients were treated under supervision with a standard six doses of dispersible formulation of AL (Coartem®, Novartis) with full cream milk. The first dose was given at the health centre by a study nurse or clinician and the subsequent doses were given at home by a parent or guardian and monitored by study personnel through mobile phone call or home visits if the parent/guardian was not reachable by mobile phone. Three children vomited within 30 min of first dose and replacement dose was given and no patient vomited a replaced dose.
Determination of plasma lumefantrine levels
From each recruited patient, 2 ml venous blood was collected into heparinized vacutainers on Day 0 and Day 7 for plasma drug level determination. The plasma was separated from blood cells by centrifugation, transferred into 2 ml cryovials and stored in liquid nitrogen. All plasma samples were sent to Muhimbili University College of Health and Allied Sciences (MUHAS) laboratory for lumefantrine level analysis by high performance liquid chromatographic (HPLC) method as described .
The study protocol was reviewed and approved by Ethical Review Committee of the Ifakara Health Institute (IHI) and MUHAS. Prior to the trial, a written consent form was signed by the parent or guardian of each participating child.
Study profile and treatment outcome
Baseline characteristics of the study participants
Haemoglobin levels on Day 0 and Day 7
Day 0 Hb(in g/dl)
Day 7 Hb (g/dl)
Plasma lumefantrine levels of patients
The lumefantrine plasma concentrations measured in patients before drug administration in all 20 enrolled patients were below HPLC detection level. The median Day 7 lumefantrine concentration level was 404.4 ng/ml (range 189.4-894.3 ng/ml). Only four (20%) patients had lumefantrine plasma concentration level < 280 ng/ml, and no patient had lumefantrine plasma concentration level <175 ng/ml. Lumefantrine concentration at day 7 was slightly decreased with unit increase in weight (kg) for any age but this decrease was not statistically significant (p-value = 0.445).
Presence of gametocytes in six children by time
Number of gametocytes per 500 WBC
D ay 14
Artemisinins are known to be highly potent anti-malarial drugs that are active against immature gametocytes [13, 14], hence pivotal in reduction of malaria transmission and elimination/eradication agenda. To the best of authors’ knowledge, there is no evidence of clinical resistance to AL. However tolerance or increased parasite clearence time and reduced susceptibility to artemisinins and artesunate-mefloquine has been observed in Thai-Cambodia and Thai-Mynmar boarder [5, 6]. This is the same locus where chloroquine resistance first emerged and subsequently spread all over the world. Emergence of artermisinin resistance would be disastrous for global malaria control. Therefore, regular surveillance and prompt response to any indication of compromised efficacy is required .
This study recorded high (100%) efficacy of AL. The reported efficacy level is more or less similar to >98% recorded in Tanzania, prior to introduction of AL , and elsewere in Africa [16–20]. However, in these other studies, the high efficacy rates were reached after exclusion of new infection by molecular genotyping. Therefore, it is obvious that the cause of alarm that prompted this exploratory study in Tabora was not malaria recurrence or compromised AL efficacy but malaria overdiagnosis. This practice has been reported previously elsewhere in Africa [21, 22] as a cause of inappropriate malaria case managment . Overdiagnosis is attributable to incompetency of laboratory workers, work overload, and pressure from patients with fever accepting malaria diagnosis .
In this study, none of the patients reported to have taken AL prior to hospital presentation, which was confirmed by lack of lumefantrine traces in the plasma. In addition, all patients had adequate therapautic levels of lumefantrine on Day 7. However, the Day 7 plasma lumefantrine levels showed wide variation between individuals. Indeed, it is known that the efficacy of AL combination is strongly influenced by wide variation in the pharmacokinetics of lumefantrine among individuals . The maximum therapeutic cure rate is achieved when the plasma drug concentration is adequately available for at least three parasite life cycles that is equal to six days . The impact of pharmacokinetic variation on selection for resistance to residual parasites in high transmission areas needs to be carefully evaluated.
Artemether-lumefantrine showed short fever and parasites clearence time and quick recovery of haemoglobin levels. Only one patient had fever up to Day 1 and neither fever nor parasitaemia was reported on Day 2, contrary to a report in central Ethiopia where fever and parasitaemia persisted to Day 3 . Haemoglobin levels significantly increased on Day 7 as reported in other studies in this region, but the second haemoglobin measurement was done on Day 14 [27, 28]. The complete 28 days’ follow up to all patients was achieved by using mobile phone calls to remind parents or guardians about their appointment dates and days. Mobile phone contacts might minimize loss to follow up in efficacy studies.
The Day 0 gametocyte prevalence (30%) recorded in this study is higher compared with those reported previously in Tanzania [29, 30]. Furthermore, as observed elsewhere in Africa [27, 31–33], no new gametocytaemic cases were observed during the follow-up period. Admittedly, this could be attributable to small sample size, which is a limitation of this study, and microscopy detection limit. Gametocytaemia persisted for seven to 14 days, (as recorded previously), against 55 days for non-ACT drugs .
ACT is effective against immature sequestered gametocytes [32, 34, 35], hence they reduce gametocyte carriage as well as gametocytes density [31, 36, 37]. Apparently, ACT does not affect the viability of mature gametocytes [31, 36]. Consequently, persistence of mature gametocytes might still play a role on maintianing transmission cycle. Hence, combination with a strong gametocytocidal drug such as primaquine will maximize ACT usefulness.
The phenomenon of increase in gametocytaemia in this study and of sexual stage count in Nigeria  despite drug treatment, might be a sign of tolerance or emergence of parasites with reduced drug sensitivity in the region. Another plausible explanation for the increase in gametocytaemia is coincidence between drug administration and maturation release of mature gametocytes into the peripheral circulation. These observations of increased gametocytaemia after treatment need to be verified in a larger study.
The therapeutic efficacy of AL against uncomplicated P. falciparum malaria was high in the study area. All patients were not exposed to AL prior to hospital presentation and levels of lumefantrine on Day 7 were therapeutically adequate but highly variable. The unusual increase in gametocytes following treatment needs be evaluated in a larger study concurrent with improvement capacity to diagnose malaria and handling of non-malarial fevers.
The study team is thankful to parents/guardians of all children who volunteered and consented to participate in this study. Our field team and health workers at St Philip’s, St Anne’s Health Centres and Kitete-Tabora Regional Hospital are highly appreciated. The authors are grateful to the MUHAS – Sida Bioanalytical laboratory colleagues who were involved in drug levels analysis. This study was part of Deokari Joseph’s MSc project which was jointly sponsored by Ifakara Health Institute (IHI), Dar Es Salaam University College of Education (DUCE), and Muhimbili Uniniversity of Health and Allied Science.
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