Habitat discrimination by gravid Anopheles gambiae sensu lato – a push-pull system
© Herrera-Varela et al.; licensee BioMed Central Ltd. 2014
Received: 5 February 2014
Accepted: 27 March 2014
Published: 2 April 2014
The non-random distribution of anopheline larvae in natural habitats suggests that gravid females discriminate between habitats of different quality. Whilst physical and chemical cues used by Culex and Aedes vector mosquitoes for selecting an oviposition site have been extensively studied, those for Anopheles remain poorly explored. Here the habitat selection by Anopheles gambiae sensu lato (s.l.), the principal African malaria vector, was investigated when presented with a choice of two infusions made from rabbit food pellets, or soil.
Natural colonization and larval survival was evaluated in artificial ponds filled randomly with either infusion. Dual-choice, egg-count bioassays evaluated the responses of caged gravid females to (1) two- to six-day old infusions versus lake water; (2) autoclaved versus non-autoclaved soil infusions; and assessed (3) the olfactory memory of gravid females conditioned in pellet infusion as larvae.
Wild Anopheles exclusively colonized ponds with soil infusion and avoided those with pellet infusion. When the individual infusions were tested in comparison with lake water, caged An. gambiae sensu stricto (s.s.) showed a dose response: females increasingly avoided the pellet infusion with increasing infusion age (six-day versus lake water: odds ratio (OR) 0.22; 95% confidence interval (CI) 0.1-0.5) and showed increasing preference to lay eggs as soil infusion age increased (six-day versus lake water: OR 2.1; 95% CI 1.4-3.3). Larvae survived in soil infusions equally well as in lake water but died in pellet infusions. Anopheles gambiae s.s. preferred to lay eggs in the non-autoclaved soil (OR 2.6; 95% CI 1.8-3.7) compared with autoclaved soil. There was no change in the avoidance of pellet infusion by individuals reared in the infusion compared with those reared in lake water.
Wild and caged An. gambiae s.l. females discriminate between potential aquatic habitats for oviposition. These choices benefit the survival of the offspring. Although the study was not designed to distinguish between stimuli that acted over a distance or on contact, it could be demonstrated that the choice of habitat is mediated by chemical cues based on both preference and avoidance. These cues, if identified, might be developed for ‘push-pull’ strategies to improve malaria vector monitoring and control.
KeywordsOviposition site selection Anopheles gambiae Natural infusion Olfactory memory
Selection of suitable oviposition sites is a critical step in the life history of mosquitoes . This is a process whereby individuals select and occupy a non-random set of aquatic habitats. Habitat selection is of major importance for the interpretation of spatial and temporal distributions of populations, and for understanding intra and inter-specific relations that influence the abundance of individuals [2, 3]. Organisms without any parental care are likely to choose habitats based on a set of innate or learned cues in order to maximize the survival and fitness of their offspring [2, 4].
Mosquitoes utilize a wide range of aquatic niches for oviposition, including natural ponds, puddles, stream fringes, marshes, tree-holes and plant axils, man-made pits, drains, rice fields, and containers . Field studies have shown that mosquitoes are discriminating in selecting sites for egg deposition [6, 7] and that oviposition choices made by gravid females are a key factor in determining larval distribution [8–11]. Although different species are found in the same type of habitat, oviposition site selectivity is considerably species specific . Immature stages of Anopheles gambiae sensu lato (s.l.), the major malaria vector in sub-Saharan Africa, are typically described as inhabiting very small, temporary sunlit pools and puddles without vegetation [13–16]. However, reviews of the literature and recent research on larval ecology have shown that this is a gross oversimplification of the wide range of habitats colonized by this species [17–19], a fact recognized over half a century ago by Holstein who reviewed the ‘extraordinary diversity of the breeding places’ of An. gambiae s.l. . Numerous studies have described how the presence of An. gambiae s.l. larvae [17, 20–23] and the capacity of individual habitats for generating adult mosquitoes [6, 8, 17, 24, 25] differs markedly over space and time, yet these surveys failed to reveal any risk factors that could consistently predict sites preferred by An. gambiae s.l.[17, 20–23]. This might lead to the conclusion that this species randomly deposits its eggs in a large range of habitats and that the heterogeneous distribution of larvae results from the survival of larvae in the aquatic habitat [9, 10] rather than the adults’ choice.
Surprisingly, fully gravid malaria vectors looking for suitable larval habitats have been grossly understudied . Compared to the wealth of knowledge of the physical and chemical factors used by gravid culicine for selecting an oviposition site [27–40] those potentially used by the world’s most deadly malaria vector remain almost unexplored. Whereas many publications recognize that the distribution of larvae between seemingly suitable aquatic habitats is probably due to the choice of the gravid female [6, 8, 24, 41–43] and that this choice probably impacts on the fitness of her offspring, there is little empirical evidence to support these assertions. Most recent research has evaluated the characteristics of aquatic habitats associated with the presence and absence of larvae [15, 21, 44–46] but the understanding of the behaviour of gravid female An. gambiae s.l. when searching for an oviposition site remains, at best, sketchy [8, 41, 47–53].
Laboratory studies demonstrated that physical conditions of the aquatic habitats influence oviposition site selection in An. gambiae s.l., with females preferring dark backgrounds to pale ones, muddy water to clear water and fully hydrated substrates [8, 41, 50, 54]. Turbidity has been suggested as an important physical cue for oviposition behaviour in An. gambiae s.l. although the evidence for this is contradictory [55, 56].
Even less is known about the chemical cues and their interaction with physical factors. Water vapour is itself an attractant to gravid mosquitoes . It has been shown that gravid An. gambiae s.l. are sensitive to bacteria-derived odours [47, 53, 58] which have been associated with increased [47, 53] and reduced  egg numbers compared to sterile media in cage bioassays. Whilst over 20 putative oviposition semiochemicals have been suggested in the literature, based on the analyses of bacteria- or habitat-derived volatile chemicals and electro-antennogram studies [53, 59], there is only one report  of two chemicals inducing a behavioural response in gravid females (one increasing and one decreasing the oviposition response).
Here the oviposition behaviour of An. gambiae s.l. was explored to test the hypotheses that a gravid An. gambiae s.l. female evaluates the suitability of a habitat using chemical cues from water bodies that oviposition choices made by a gravid female benefit the offspring and that this choice cannot be modified by experience in one generation.
Habitat selection by gravid An. gambiae s.l. was tested by presenting a choice of two infusions; one made with soil from an area where natural habitats occur frequently, and one made with rabbit food pellets. Rabbit food pellets are frequently used as diet for mosquito larvae in insectaries [61, 62] and infusions made of grass, hay and other plant material, including rabbit food pellets have shown to be attractive to a range of mosquito species and have been used in gravid traps [63–66]. The aim was to explore whether Anopheles gambiae s.l. might also be drawn to this infusion.
Natural colonization and larval survival was evaluated in artificial ponds filled randomly with either infusion. As a consequence of the field observations, two-choice, egg-count bioassays were used to explore pattern of oviposition seen in the field. Experiments were designed to address the following questions: 1) Do gravid An. gambiae s.l. females discriminate between different habitats when searching for an oviposition site? 2) Does the oviposition choice benefit the survival of their offspring? 3) Are gravid females guided by preference (attractants/stimulants) or avoidance (repellents/deterrents)? 4) Are oviposition choices likely to be based on chemical cues? and, 5) Is the choice made by a gravid female influenced by her olfactory memory of her larval habitat?
Experiments were carried out at the International Centre for Insect Physiology and Ecology (icipe), Mbita, on the shores of Lake Victoria, Western Kenya (geographic coordinates 0°26′06.19″ South; 34°12′53.13″ East; altitude 1,137 m above sea level). Mbita has a typical tropical climate; temperatures oscillate between 18-28°C and there is annual rainfall of 1,436 mm (based on data from icipe meteorological station for 2010–2012). Two rainy seasons occur annually, the long rainy season between March and June and the short rainy season between October and December. Malaria is endemic in the area and transmitted by three vectors, which are in order of their abundance: Anopheles arabiensis, Anopheles gambiae sensu stricto (s.s.) and Anopheles funestus.
Open-field trials were conducted with wild anopheline and culicine females that oviposited in tubs of water sunk into the ground. These were colonized within three days. Laboratory experiments were carried out with insectary-reared An. gambiae s.s. (Mbita strain) supplied by icipe’s insectary and reared following standard operating procedures. Briefly, larvae were reared in round plastic tubs (diameter 0.6 m) filled with water from Lake Victoria and fed Tetramin® fish food twice daily. Larvae were collected randomly from several tubs on the day of experiment. Gravid mosquitoes were prepared by selecting 300 female and 300 male mosquitoes, two to three days old, from their rearing cages at 12.00 and keeping them in 30 × 30 × 30 cm netting cages at 25-28°C and 68-75% relative humidity. To avoid mosquito desiccation, cotton towels (folded to 25×12 cm) were saturated with lake water and placed over the cages. Mosquitoes were starved of sugar for seven hours before blood feeding and allowed to feed on a human arm for 15 min at 19.00 on the same day. After feeding, mosquitoes were provided with 6% glucose solution ad libitum. This procedure was repeated 24 hours later. After the first blood meal unfed female mosquitoes were removed from the cages. Fed female mosquitoes were kept together with males for two days after the second blood meal before using them in an experiment (i e, females four to five days after first blood meal). In the afternoon (16.30) of the day of an experiment 45–100 (depending on experiment and availability) visually presumed gravid females, that is with an enlarged, pale white abdomen, were selected from the holding cage. A small percentage of these mosquitoes were probably not gravid because most females needed two blood meals to reach full gravidity and some never reach full gravidity even after three feeds [68, 69]. Whilst two meals were provided it cannot be guaranteed that two meals were taken by all females. This might be the reason that not all mosquitoes exposed to oviposition medium in experiments laid eggs (responded), therefore the number of responders was smaller than the number tested. Non-responders were excluded from the analyses.
Do gravid Anopheles gambiae sensu lato females discriminate between different habitats when searching for an oviposition site?
To explore natural colonization of habitats by wild mosquitoes, 20 artificial habitats were created by implanting 20 plastic tubs (40 cm diameter, 20 cm deep) into an open-sunlit field during the long rainy season in May 2011. The tubs were placed in four lines of five tubs each 4 m apart . Two different substrates were randomly offered in the artificial habitats. Half of the tubs (ten) received 30 g of rabbit food pellets (Scooby® rabbit and rodent food, Nairobi) containing hay and grains from maize, wheat, barley, cotton, sunflower, soya bean meal, and traces of molasses, vitamins and minerals. The remaining half of the tubs (ten) received 2 kg of dry soil taken from a field at icipe. Soil texture was characterized as a silty clay loam according to the US Department of Agriculture (USDA) texture triangle  using the detergent method  to separate and quantify soil mineral particles of different size. A volume of 15 l of lake water pumped from Lake Victoria, was added to each tub and the water level was held constant by adding water to the 15 l mark daily. The two treatments are henceforth referred to as pellet and soil infusion. To study the oviposition response of wild mosquitoes the tubs were monitored daily between 08.00 and 10.00 by dipping five times per tub with a standard dipper (350 ml). Two different dippers were used for the two treatments to avoid contamination. Four dips were taken from the edge of the tubs and one from the middle. The content of each dip was emptied into a white plastic bowl and all early instars (first and second stage larvae) counted and recorded for both culicine and anopheline mosquitoes. All larvae and the water were returned to the respective tub. The tubs were followed for 16 days. Ponds were searched for pupae and collected daily to prevent any emergence of potential disease vectors. Pupae were allowed to emerge in cages in the laboratory and any anophelines emerging identified to species using morphological keys [13, 73] and for specimens of the An. gambiae complex using the ribosomal DNA-polymerase chain reaction (PCR) method to distinguish between the two local species of the complex An. gambiae s.s. and An. arabiensis.
Does the oviposition choice benefit the survival of their offspring?
Larval survival was assessed by introducing individual, insectary-reared, first instar An. gambiae s.s. larvae in infusions collected from the tubs set up in the field. Infusion samples were taken after one, six, 11, and 16 days. One-hundred ml of infusion was collected from each of the ten tubs per treatment and pooled per treatment (soil or pellet infusion) in a plastic bottle. Lake water was used as a control. First instars were introduced in 100 ml plastic cups containing 50 ml of pellet infusion, soil infusion or lake water. Twenty larvae were exposed individually per treatment and collection day. Larvae were fed every second day with finely ground Tetramin® Baby fish food. Food was provided with a blunt toothpick that was first wetted in lake water and then dipped quickly, not more than 1 mm deep into the ground food, and then dipped onto the surface of the test water. Larval development was monitored daily and the time of death or time to pupation and emergence recorded. This experiment was implemented under ambient conditions in a semi-field system (80 sq m) with screened walls and a glass roof .
Are gravid females guided by preferences or avoidance?
Based on the analysis of the field data a series of two-choice, egg-count bioassays were designed to investigate if the response of wild gravid females observed in the field was based on avoidance or preference of an infusion or both.
Summary details of dual-choice, egg-count bioassays to evaluate oviposition choices in Anopheles gambiae sensu stricto
Dual-choice cage, egg-count bioassays
No of rounds
Total no of females responding for all rounds (total number set up)
Set 1: Pellet infusions
2-day old pellet infusion
4-day old pellet infusion
6-day old pellet infusion
Set 2: Soil infusions
2-day old soil infusion
4-day old soil infusion
6-day old soil infusion
Set 3: Vision versus olfaction in soil infusions
6-day old soil infusion
Autoclaved 6-day old soil infusion
Autoclaved 6-day old soil infusion
6-day old soil infusion
Set 4: Olfactory memory – pellet infusions
Lake water reared An. gambiae females
6 day old pellet infusion
Pellet infusion reared An. gambiae females
6-day old pellet infusion
Infusions were prepared in a similar way as for the field tests. Fifteen l of lake water were either incubated with 30 g of pellets to prepare a pellet infusion or incubated with 2 kg of soil to prepare a soil infusion. Infusions were prepared in a plastic tub (40 cm diameter 20 cm depth) six days, four days and two days before the day of experiment in order to test all ages in parallel. Tubs were covered with mosquito netting and kept in makeshift sheds at ambient conditions but protected from rain. Experiments were implemented over three to nine rounds depending on the availability of gravid females and the response rate per round (Table 1) with fresh batches of infusions and different batches of mosquitoes for every round. On the day of experiment infusions were sieved through a clean piece of cotton cloth to remove large debris remaining from the pellets or soil.
Fifteen to 25 replicate cages per treatment were set up per round. A single gravid female was introduced per cage at 17.30. The next morning between 08.00 and 09.00 the absence or presence and the number of eggs was recorded for the control and test cup in each cage.
Turbidity, conductivity, dissolved oxygen, and pH were measured in five cups per treatment in four different batches of pellet, soil infusions and lake water using a turbidity meter (TURB 355IR, WTW Germany) and a multimeter (Multi 340i, WTW, Germany). In addition one batch of pellet, soil infusion and lake water was tested for ammonium (NH4+), carbonate hardness, total hardness, nitrate (NO3−), nitrite (NO2−), and phosphate (PO43−) content using Aquamerck® test kits from the compact laboratory for water testing (Aquamerck® No.111151, Germany).
Are oviposition choices likely to be based on chemical cues?
Soil infusions differed strongly in colour and turbidity from lake water. To assess if the oviposition response observed was based on visual or chemical cues, a third set of dual-choice, egg-count bioassays were implemented with six-day old soil infusions (Table 1, Set 3) comparing the relative attractiveness of autoclaved and non-autoclaved infusion [47, 76]. The experiment followed the same experimental procedures as described above. After filtering the infusion through a cloth on the day of experiment, the infusion was split in two equal volumes and half autoclaved at 120°C for 20 min to kill bacteria potentially involved in releasing oviposition semiochemicals [47, 77] and to reduce the amount of volatile chemicals from the solution whilst maintaining the colour and turbidity of the infusion. After autoclaving, the infusion was left to cool to ambient temperature before setting up the cage bioassays. The oviposition choice of individual gravid females was evaluated for six-day old soil infusion versus lake water, autoclaved six-day old soil infusion versus lake water and for autoclaved versus non-autoclaved six-day old infusion. An equal number of cages were set up where both cups in a cage contained lake water and were randomly labelled as control and test cup.
In order to confirm that autoclaving sterilized the infusion, samples (1 ml) of both infusions were taken during each experimental round for bacterial cultures. Samples were serially diluted (ten-fold) two times in distilled water. After dilution, 100 μl of each of the ×1 (undiluted), ×10−1 and ×10−2 dilutions was spread separately onto the surface of duplicate Lysogeny Broth (LB) agar-plates (LB Lennox-Fisher Scientific) . Plates were incubated overnight at 30°C and the presence of colonies recorded.
The same physical and chemical parameters were measured for the autoclaved infusion as described above for the non-autoclaved pellet and soil infusions.
Is the choice of the gravid female influenced by her olfactory memory of her larval habitat?
A fourth set of experiments (Table 1, Set 4) was designed to assess the possibility that a gravid female’s choice for an oviposition site might be influenced by her olfactory memory of her larval habitat, as has been suggested for culicine species [4, 79].
To test this, approximately 2,000 An. gambiae s.s. eggs were dispensed in 1.5 l of two-day old pellet infusion and another 2,000 eggs in lake water and reared under the same conditions to the adult stage. The infusion and lake water in the rearing pans was replaced every two days with fresh two-day old infusion or lake water until all surviving larvae pupated. Larvae were fed with Tetramin® fish food twice daily following routine insectary procedures. Pupae were collected in a cup with 100 ml of rearing water and placed in 30×30×30 cm cages for emergence. Gravid females for cage bioassays were obtained as described above.
Dual-choice cage bioassays were carried out in parallel with gravid An. gambiae s.s. reared in the infusion and gravid An. gambiae s.s. reared in lake water. A single mosquito was offered a choice between six-day old pellet infusion or lake water. Forty-five replicates were set up in parallel for both treatment groups as described above.
Sample size considerations
The sample size (number of responders) in the four sets of cage experiments differed for a number of reasons. Due to adverse climate conditions affecting the mosquito supply during the pellet infusion bioassays, the production of colony-reared mosquitoes was low. Nevertheless, two-sample comparison of proportions power calculation showed that 66 responders in each arm in the pellet infusion bioassays (Table 1, Set 1) was sufficient to detect a 23% increase or decrease in the proportion of eggs laid in the treatment compared to the lake water only experiment with 80% power at the 5% level of significance. The effect of the pellet infusion observed on oviposition response was much stronger than 23%. In the soil infusion experiments (Table 1, Set 2 and Set 3), a minimum of 150 responders in each arm was analysed. This was sufficient to detect a 15% increase or decrease in the proportion of eggs laid in the treatment as compared to the lake water only experiment at the same power and significance level. This level of accuracy was deemed appropriate for investigating significant behavioural cues affecting the oviposition choice. The evaluation of olfactory memory required the mosquitoes to be reared in pellet infusion where larval mortality was nearly 98%. Therefore, only 45 females could be tested, out of which only 31 and 37 responded in the two arms (Table 1, Set 4). The hypothesis for this experiment was that the preference of gravid females could be changed and therefore at least double the proportion of eggs laid in pellet infusion by infusion-reared females as compared to the lake water-reared females. With 31 responders in each arm the experiment was powered (80%) to detect a change in the proportion of 35%.
All data were analysed in R statistical software version 2.13.1 . The one sample proportion test function was used to estimate the 95% confidence intervals (CI) for the proportion of larvae surviving in pellet infusion, soil infusion and lake water. Pupation time of larvae exposed to different treatments was calculated using the following formula: (A×1) + (B×2) + (C×3)…(G×10)/Total number of pupae collected, where A, B, C…G are the number of pupae collected on day 1, 2, 3 to 10. Dual-choice, egg-count bioassays were analysed using generalized linear models (glm-function) with a quasibinomial distribution fitted to account for the overdispersion. In the first three sets of experiments the proportion of eggs laid in test cups in the cages with equal treatments (lake water in both cups) were compared with the proportion of eggs laid in test cups in cages with two different treatments. It was hypothesized that gravid females presented with an identical treatment lay in both cups in an approximately equal proportion (p = 0.5). The statistical analysis aimed to reveal if the test treatment of interest (e g, infusions of different age) received an increased or decreased proportion of the total number of eggs laid as compared to the lake water only treatment. Therefore, the treatment choice (e g, lake water only cages, cages with infusion versus lake water) and the round of experiment were included as fixed factors to analyse their impact on the outcome (proportion of eggs laid in test cup). A similar analysis was used for the fourth set of experiments to compare the proportion of eggs laid in test cups (pellet infusion) by gravid females that were reared in hay infusion during their larval development, compared to the proportion of eggs laid in test cups by gravid females that were reared in lake water. The mean proportion of eggs laid in test cups in different treatments and their 95% CIs were calculated as the exponential of the parameter estimates for models with no intercept included. Similarly, multiple comparisons of treatments were calculated based on the model parameter estimates.
Gravid Anopheles gambiae sensu lato females discriminate between habitats when searching for an oviposition site
All pupae collected from the artificial habitats belonged to the An. gambiae complex. PCR-based species analysis revealed that nearly all the wild An. gambiae s.l. were An. arabiensis (98%, 49/50).
The female’s oviposition choice benefits the survival of her offspring
Gravid female Anopheles gambiae sensu stricto show avoidances and preferences when selecting an oviposition site
On average individual females laid 63 eggs (95% CI 60–65) (Figure 5) irrespective of the experiment and treatment. Notably, 18% (95% CI 11-26%) of gravid females laid eggs in both cups provided in a cage, a behaviour known as skip oviposition in other mosquito species  but rarely reported for An. gambiae s.s. . The average number of eggs laid by skip-ovipositing females and by females that chose only a single cup was similar. Whilst the percentage of skip-ovipositing females was similar in all treatments with two equal lake water choices and in all soil infusion treatments, this behaviour was affected by the pellet infusion. Only a few An. gambiae s.s. females skip-oviposited in the four- and six-day old pellet infusion treatments (6%, 95% CI 3-9%).
Physical and chemical properties of pellet and soil infusions
Oviposition substrates in choice experiments
Dissolved oxygen (mg/l)
Carbonate hardness (mmol/l)
Total hardness (mmol/l)
The increased carbon and total hardness of the pellet infusion corresponded with the increased conductivity levels and the high ammonium and phosphate levels compared with the soil infusion (Table 2).
Chemical cues from the infusions are responsible for the oviposition choice in cage bioassays
Since soil infusions differed in appearance from the lake water, an additional set of experiments was carried out to evaluate whether the attractiveness of this infusion was due to visual cues. Cage experiments with two equal choices of lake water confirmed an equal distribution of eggs between control and test cup. Notably, when gravid females had a choice between lake water and autoclaved soil infusion, the lake water was preferred. The oviposition preference for six-day old soil infusion compared with lake water was also confirmed in this set of experiments with nearly identical odds ratios as before of 2.2. The preference for the six-day old infusion was corroborated when given a choice between autoclaved and non-autoclaved infusions of similar colour and turbidity (Figure 5, Set 3).
Results from this set of experiments suggest that chemical cues are involved in the oviposition responses observed. If the preference for the six-day old soil infusion over lake water was based on turbidity and/or colour of the infusion alone, a similar response in the choice tests with autoclaved versus non-autoclaved infusion should have been seen as in the choice tests with lake water versus autoclaved infusion. Due to the slight avoidance of the autoclaved infusion the odds of finding an egg in the non-autoclaved six- day old infusion should have been approximately 1.3 (decreased choice for autoclaved infusion by 30% or increased choice of fresh infusion by 30%). Nevertheless, the remaining odds of 2.2 can only be explained by chemical cues being either involved in attracting the female from a short distance or stimulating the female to lay eggs on contact with water. Physical and chemical water parameters were similar for autoclaved and non-autoclaved soil infusions. Bacteria cultures from autoclaved infusions confirmed that samples did not contain any bacteria that could grow on LB plates as opposed to the non-autoclaved infusion where colonies of at least three different morphologies were observed.
The oviposition choice of the gravid female is not influenced by her olfactory memory of her larval habitat
The results confirm that wild and caged An. gambiae s.l. females discriminate (defined as recognition and understanding of the difference between two things ) between potential aquatic habitats for oviposition and make clear choices when presented with contrasting oviposition media. These choices benefit the survival of the offspring. Although the experimental design does not allow a conclusion whether the stimuli acted over a distance (attractants and repellents ) or on contact with the oviposition medium (stimulants and deterrents ), it could be demonstrated that the choice of breeding site is guided by both avoidance and preference. However, the exclusive way in which the artificial ponds were chosen in the field, where they were set up relatively close to each other, suggest that these characteristics were detected by both culicines and anophelines from a distance rather than on contact.
Muddy water has previously been suggested to increase oviposition response of gravid An. gambiae s.s. in cages when offered together with clear water , however in this study cage experiments could not confirm this observation. The difference in turbidity between lake water and infusions cannot explain the avoidance and preference observed at short range in the cages. Two-day and six-day old pellet infusions did not differ in their turbidity but significantly differed in the oviposition response they received. Similarly, all soil infusions should have elicited equally strong responses from gravid females if turbidity was an important oviposition cue at short range. On the contrary, the six-day old soil infusion remained equally preferred for oviposition when tested against a turbid and autoclaved infusion than when tested against clear lake water. The results suggest that chemical and not visual cues were responsible for the responses observed in the cage experiments. The previously published preference of muddy water  may have been based on chemical cues associated with the muddy water which was taken from a natural habitat. However the possibility that visual cues played a role in the selection of oviposition sites by wild mosquitoes in field experiments especially when searching for water bodies from a distance cannot be entirely excluded.
It is likely that the chemical cues used by mosquitoes to avoid pellet infusions and to prefer soil infusions were at least partly of microbial origin, which is supported by the lack of attraction to the autoclaved soil infusion compared to clear lake water. It is most likely that the observed oviposition choices as well as the larval survival were rooted in the water quality of the habitat and consequently the associated micro-organisms and chemicals in the water. The physical and chemical water parameters measured for the two infusions suggest that they represented aquatic habitats in different stages of decay. Contrary to the expectation at the onset of the experiment, pellet infusions created with increasing age a habitat type in a severe state of decomposition. High ammonia and phosphate levels are characteristic of recently inundated organic material . The odour of the pellet infusion is associated with fermentation of organic material by facultative and anaerobic bacteria. This leads to depletion of the oxygen supply and a decrease in pH as a result of accumulation of organic acids in the water [87–89]. The soil infusion had the characteristics of a less nutrient-rich habitat containing relatively little organic matter. This limits the removal of oxygen by aerobic heterotrophic micro-organisms and hence the water column will stay aerobic .
Anaerobic fermentation products of organic matter have been previously shown to be highly attractive to a number of gravid culicine species such as Culex stigmatosoma (formerly peus) , Culex quinquefasciatus[33, 65, 90–92], Culex pipiens, Aedes aegypti[77, 93] and Aedes albopictus[94, 95]. These infusions have been associated with a range of bacteria such as Aerobacter aerogenes, Pseudomona aeruginosa, Bacillus cereus[96–98] and volatile chemicals produced by them including 4- methylphenol, 3-methylindole, carboxylic acids and methyl esters [33, 39, 99, 100]. It is likely that similar factors were responsible for the strong repellent/deterrent effect on gravid An. gambiae s.l. females.
Most stagnant water bodies will show increasing signs of decomposition over time but the speed and extent of this will depend largely on habitat quality . Therefore, it is argued that habitat age or permanence alone is not a good predictor for the oviposition response of An. gambiae s.l. as has been suggested . For example, the content and input of organic matter, source of water and frequency of fresh water inflow will affect the composition of the biotic community and chemical and physical characteristics of an aquatic habitat [86, 89, 101]. This might explain why in some environments semi-permanent and permanent habitats are just as well colonized as temporary habitats traditionally thought to be the preferred An. gambiae s.l. habitats [13, 17, 67]. Habitats made of pellet infusion were avoided by Anopheles from an early habitat age, whilst interestingly, the highest preference of the soil infusion was recorded on day 6 in the laboratory and between day 6 and 10 in the field after the habitats were well established, contradicting the idea that An. gambiae s.l. is a pioneer species colonizing temporary habitats immediately after their occurrence .
Typically, it is reported that An. gambiae s.l., although largely a generalist, is not found in heavily polluted waters [19, 102]. Hancock  further observed that An. gambiae s.l. avoided water with a low pH when it was also accompanied with high organic matter content. Addition of freshly cut vegetation (i e, grass cuttings) to aquatic habitats has also been shown to prevent the larval development of An. gambiae s.l.. The results from the experiments with pellet infusions support these observations. On the other hand, there have been recent reports of An. gambiae s.l. colonizing polluted habitats especially in urban areas [104–106]. Clearly, the degree of avoidance or acceptance of a polluted habitat by An. gambiae s.l. depends on the extent and nature of pollution . Results show that two-day old pellet infusions were not rejected by Anopheles and even four-day old infusions still received a considerable proportion of the oviposition responses despite their adverse water characteristics. This supports the idea that An. gambiae s.l. has a very high tolerance level of what they accept as oviposition sites, especially in the absence of better alternatives in close vicinity as is often the case in urban environments and in contrast to the here presented field experiment where good habitats were offered right next to the unfavoured ones.
Importantly, An. gambiae s.l. appears to have an innate propensity to avoid specific chemical cues that were emitted from the pellet infusion. Rearing An. gambiae s.s. from egg to pupae in this infusion did not alter this behaviour. Gravid females that had experienced the pellet infusion during larval development avoided the infusion for oviposition as much as the females that had no prior experience of it. This suggests that the environment in which An. gambiae s.s. develop as larvae does not determine the preferred oviposition site when they return to lay eggs. This is in contrast to published work on Cx. quinquefasciatus where it was demonstrated that rearing the larvae in an infusion made from guinea-pig faeces cancelled their innate preference for a hay infusion .
The cage bioassays with individual gravid females allowed a number of interesting observations that are rarely reported since the majority of studies with An. gambiae s.s. are done with groups of mosquitoes where the actual number of females laying per cage is unknown . The occurrence of skip-oviposition in gravid An. gambiae s.s. and how this is affected by chemical cues was demonstrated. Furthermore, the design revealed that the mean number of eggs laid per female in a cage was similar irrespective of the experiment and treatment; only the distribution between cups differed when two different choices were presented. This indicates that gravid females did not retain their eggs in the presence of an unfavoured substrate when they were offered a suitable alternative choice. It also shows that the preferred soil infusion did not stimulate individual females to lay more eggs than they would do in lake water. Testing individual females also excludes potential aggregation effects. Whilst from the field experiments it might have been possible that gravid females selected habitats that already received eggs from conspecific females, cage bioassays with individual females showed the same avoidance and preference behaviour as observed in the field, confirming that conspecifics alone cannot explain the observed choice.
The potential involvement of microbial activity in breaking down organic matter and producing semiochemicals that impact on the oviposition responses of gravid An. gambiae s.s. was deduced partly by the lack of attraction of An. gambiae s.s. to a sterile soil infusion. However, this must be interpreted with caution since autoclaving the infusion might not only have killed the microbes but affected the chemistry of the resulting infusion, possibly altering the response of gravid mosquitoes by chemical changes rather than biological changes .
Batch-to-batch variations were recorded in the response of gravid mosquitoes to the infusions, resulting, for example, in some rounds showing a high preference and other rounds only a moderate preference for the soil infusion. This variation can be attributed to differences in the quality and amounts of odorants released from the infusions and stochastic events. Fresh infusions were prepared for every test round with different batches of pellets and soil. Especially, for the soil it is highly likely that there were differences in the soil condition as well as differences in the species composition of the microbial community associated with the natural materials over time. It has been shown previously that natural infusions can be an inconsistent source of odorants for oviposition site-seeking mosquitoes and therefore every batch needs to be verified to be behaviourally active before it can be used for subsequent experiments . Ideally, if info-chemicals were to be used for monitoring and/or controlling gravid malaria vectors, specific chemically defined oviposition cues would be preferred over natural infusions to ensure a consistent response in gravid females either pushing them away from human population [107, 108] or pulling them towards a gravid trap [38, 109].
Whilst the observed avoidance behaviour towards the organically rich pellet infusion was strong and in the same range as reported for other species in response to unfavourable chemical cues [107, 108, 110], the observed preference in the cages for the soil infusion was relatively weak and it is questionable whether it could compete with other suitable habitats from a larger distance. Nevertheless, consistent response derived from over 150 replicates in two experiments likely represents a genuine effect. Further investigations are in progress to characterize the bacteria communities associated with the infusions and the volatile chemicals emitted from the infusions and detected by gravid An. gambiae s.s. using gas-chromatography coupled to mass-spectrometry and coupled gas chromatography-electroantennogram detection.
It must be cautioned that not all soils and all rabbit food pellets will lead to the same physical and chemical parameters as the infusions presented here. Therefore the two infusions of this study only serve as specific examples for two highly contrasting media. Further work is needed to screen other soil samples to see if the observed response is a response common for all soil infusions prepared under standard conditions and if the same bacteria and chemical profiles can be detected, or, which is more likely, that there are significant differences depending on the source of the soil.
This work illustrates that a gravid An. gambiae s.l. female selects a suitable habitat for oviposition using chemical cues from water bodies. It furthermore emphasizes that natural infusions can be used to manipulate the oviposition behaviour of An. gambiae s.l.. Soil infusions have the potential to be used to bait gravid traps for the collection of An. gambiae s.l., although further work must be implemented to elucidate whether the observed preference was based on the specific soil type tested or whether similar responses can be achieved with any soil. The low An. gambiae s.l. catching efficacy reported for gravid traps operationally used for Culex and Aedes monitoring might partly be explained by the infusions routinely used in these traps, i e, fermented hay infusions, rabbit food pellet and cow manure infusions [64–112]. The identification of the chemicals responsible for the preference of the soil infusion might be exploited to bait gravid traps specifically for the collection of An. gambiae s.l.
We thank David Alila, Elisha Obudho and Benard Oyembe for providing mosquitoes for this study and Paul Ouma, Elizabeth Masinde, Gregory Masinde and Rose Atieno for their technical support. We also extend our gratitude to Benedict Orindi for statistical advice. MH-V was supported by the Colombian Department of Science, Technology and Innovation (COLCIENCIAS) through the Scholarship programme “Francisco Jose de Caldas”. This project received funding from the National Institute of Health (NIH) through grant no. R01AI082537.
- Refsnider JM, Janzen FJ: Putting eggs in one basket: ecological and evolutionary hypotheses for variation in oviposition-site choice. Annu Rev Ecol Evol Syst. 2010, 41: 39-57. 10.1146/annurev-ecolsys-102209-144712.Google Scholar
- Rejmankova E, Higashi R, Grieco J, Achee N, Roberts D: Volatile substances from larval habitats mediate species-specific oviposition in Anopheles mosquitoes. J Med Entomol. 2005, 42: 95-103. 10.1603/0022-2585(2005)042[0095:VSFLHM]2.0.CO;2.PubMedGoogle Scholar
- Morris DW: Toward an ecological synthesis: a case for habitat selection. Oecologia. 2003, 136: 1-13. 10.1007/s00442-003-1241-4.PubMedGoogle Scholar
- McCall PJ, Eaton G: Olfactory memory in the mosquito Culex quinquefasciatus. Med Vet Entomol. 2001, 15: 197-203. 10.1046/j.0269-283x.2001.00304.x.PubMedGoogle Scholar
- Laird M: The natural history of larval mosquito habitats. 1988, London: Academic PressGoogle Scholar
- Muirhead-Thomson RC: Studies on the breeding places and control of Anopheles gambiae and A. gambiae var. melas in coastal districts of Sierra Leone. Bull Entomol Res. 1945, 38: 527-558.Google Scholar
- Macan TT: Factors that limit the range of freshwater animals. Biol Rev Camb Philos Soc. 1961, 36: 151-198. 10.1111/j.1469-185X.1961.tb01582.x.PubMedGoogle Scholar
- Bates M: Oviposition experiments with anopheline mosquitoes. Am J Trop Med Hyg. 1940, 20: 569-583.Google Scholar
- Muirhead-Thomson RC: Studies on the behaviour of Anopheles minimus. Part I. The selection of the breeding place and the influence of light and shade. J Malar Inst India. 1940, 3: 265-294.Google Scholar
- Muirhead-Thomson RC: Studies on the behaviour of Anopheles minimus. Part II. The influence of water movement on the selection of the breeding place. J Malar Inst India. 1940, 3: 295-322.Google Scholar
- Wallis RC: A study of oviposition activity of mosquitoes. Am J Hyg. 1954, 60: 135-168.PubMedGoogle Scholar
- Bentley MD, Day JF: Chemical ecology and behavioral aspects of mosquito oviposition. Annu Rev Entomol. 1989, 34: 401-421. 10.1146/annurev.en.34.010189.002153.PubMedGoogle Scholar
- Gillies MT, De Meillon B: The Anophelinae of Africa South of the Sahara. 1968, Johannesburg: Publications of the South African Institute of Medical Research, 54-Google Scholar
- Muirhead Thomson RC: Mosquito Behaviour in Relation to Malaria Transmission and Control in the Tropics. 1951, London: Edward Arnold & CoGoogle Scholar
- Gimnig JE, Ombok M, Kamau L, Hawley WA: Characteristics of larval Anopheline (Diptera: Culicidae) habitats in western Kenya. J Med Entomol. 2001, 38: 282-288. 10.1603/0022-2585-38.2.282.PubMedGoogle Scholar
- Minakawa N, Sonye G, Yan G: Relationships between occurrence of Anopheles gambiae s.l. (Diptera: Culicidae) and size and stability of larval habitats. J Med Entomol. 2005, 42: 295-300. 10.1603/0022-2585(2005)042[0295:RBOOAG]2.0.CO;2.PubMedGoogle Scholar
- Fillinger U, Sonye G, Killeen GF, Knols BG, Becker N: The practical importance of permanent and semipermanent habitats for controlling aquatic stages of Anopheles gambiae sensu lato mosquitoes: operational observations from a rural town in western Kenya. Trop Med Int Health. 2004, 9: 1274-1289. 10.1111/j.1365-3156.2004.01335.x.PubMedGoogle Scholar
- Fillinger U, Lindsay SW: Larval source management for malaria control in Africa: myths and reality. Malar J. 2011, 10: 353-10.1186/1475-2875-10-353.PubMed CentralPubMedGoogle Scholar
- Holstein MH: Biology of Anopheles gambiae: Research in French West Africa. 1954, World Health Organization (Geneva)Google Scholar
- Majambere S, Fillinger U, Sayer DR, Green C, Lindsay SW: Spatial distribution of mosquito larvae and the potential for targeted larval control in The Gambia. Am J Trop Med Hyg. 2008, 79: 19-27.PubMedGoogle Scholar
- Fillinger U, Sombroek H, Majambere S, van Loon E, Takken W, Lindsay SW: Identifying the most productive breeding sites for malaria mosquitoes in The Gambia. Malar J. 2009, 8: 62-10.1186/1475-2875-8-62.PubMed CentralPubMedGoogle Scholar
- Munga S, Yakob L, Mushinzimana E, Zhou G, Ouna T, Minakawa N, Githeko A, Yan G: Land use and land cover changes and spatiotemporal dynamics of anopheline larval habitats during a four-year period in a highland community of Africa. Am J Trop Med Hyg. 2009, 81: 1079-1084. 10.4269/ajtmh.2009.09-0156.PubMed CentralPubMedGoogle Scholar
- Ndenga BA, Simbauni JA, Mbugi JP, Githeko AK, Fillinger U: Productivity of malaria vectors from different habitat types in the western Kenya highlands. PLoS. 2011, 6: e19473-10.1371/journal.pone.0019473.Google Scholar
- Muirhead-Thomson RC: Studies on salt-water and fresh-water Anopheles gambiae on the east african coast. Bull Entomol Res. 1951, 41: 487-502. 10.1017/S0007485300027772.Google Scholar
- Mutuku FM, Bayoh MN, Gimnig JE, Vulule JM, Kamau L, Walker ED, Kabiru E, Hawley WA: Pupal habitat productivity of Anopheles gambiae complex mosquitoes in a rural village in western Kenya. Am J Trop Med Hyg. 2006, 74: 54-61.PubMedGoogle Scholar
- Ferguson HM, Dornhaus A, Beeche A, Borgemeister C, Gottlieb M, Mulla MS, Gimnig JE, Fish D, Killeen GF: Ecology: a prerequisite for malaria elimination and eradication. PLoS Med. 2010, 7: e1000303-10.1371/journal.pmed.1000303.PubMed CentralPubMedGoogle Scholar
- Laurence BR, Pickett JA: erythro-6-Acetoxy-5-hexadecanolide, the major component of a mosquito oviposition attractant pheromone. J Chem Soc Chem Commun. 1982, 0: 59-60.Google Scholar
- Beehler JW, Millar JG, Mulla MS: Protein hydrolysates and associated bacterial contaminants as oviposition attractants for the mosquito Culex quinquefasciatus. Med Vet Entomol. 1994, 8: 381-385. 10.1111/j.1365-2915.1994.tb00103.x.PubMedGoogle Scholar
- Beehler JW, Millar JG, Mulla MS: Field evaluation of synthetic compounds mediating oviposition in Culex mosquitoes (Diptera: Culicidae). J Chem Ecol. 1994, 20: 281-291. 10.1007/BF02064436.PubMedGoogle Scholar
- Isoe J, Beehler JW, Millar JG, Mulla MS: Oviposition responses of Culex tarsalis and Culex quinquefasciatus to aged Bermuda grass infusions. J Am Mosq Control Assoc. 1995, 11: 39-44.PubMedGoogle Scholar
- Isoe J, Millar JG: Characterization of factors mediating oviposition site choice by Culex tarsalis. J Am Mosq Control Assoc. 1995, 11: 21-28.PubMedGoogle Scholar
- Millar JG, Chaney JD, Beehler JW, Mulla MS: Interaction of the Culex quinquefasciatus egg raft pheromone with a natural chemical associated with oviposition sites. J Am Mosq Control Assoc. 1994, 10: 374-379.PubMedGoogle Scholar
- Millar JG, Chaney JD, Mulla MS: Identification of oviposition attractants for Culex quinquefasciatus from fermented Bermuda grass infusions. J Am Mosq Control Assoc. 1992, 8: 11-17.PubMedGoogle Scholar
- Bentley MD, McDaniel IN, Yatagai M, Lee HP, Maynard R: p-Cresol: an oviposition attractant of Aedes triseriatus. Environ Entomol. 1979, 8: 206-209.Google Scholar
- Hwang Y, Schultz GW, Axelrod H, Kramer WL, Mulla MS: Ovipositional repellency of fatty acids and their derivatives against Culex and Aedes mosquitoes. Environ Entomol. 1982, 11: 223-226.Google Scholar
- Mendki MJ, Ganesan K, Prakash S, Suryanarayana MVS, Malhotra RC, Rao KM, Vaidyanathaswamy R: Heneicosane: an oviposition-attractant pheromone of larval origin in Aedes aegypti mosquito. Curr Sci. 2000, 78: 1295-1296.Google Scholar
- Ganesan K, Mendki MJ, Suryanarayana MVS, Prakash S, Malhotra RC: Studies of Aedes aegypti (Diptera: Culicidae) ovipositional responses to newly identified semiochemicals from conspecific eggs. Aust J Entomol. 2006, 45: 75-80. 10.1111/j.1440-6055.2006.00513.x.Google Scholar
- Seenivasagan T, Sharma KR, Sekhar K, Ganesan K, Prakash S, Vijayaraghavan R: Electroantennogram, flight orientation, and oviposition responses of Aedes aegypti to the oviposition pheromone n-heneicosane. Parasitol Res. 2009, 104: 827-833. 10.1007/s00436-008-1263-2.PubMedGoogle Scholar
- Ponnusamy L, Xu N, Nojima S, Wesson DM, Schal C, Apperson CS: Identification of bacteria and bacteria-associated chemical cues that mediate oviposition site preferences by Aedes aegypti. Proc Natl Acad Sci USA. 2008, 105: 9262-9267. 10.1073/pnas.0802505105.PubMed CentralPubMedGoogle Scholar
- Ponnusamy L, Wesson DM, Arellano C, Schal C, Apperson CS: Species composition of bacterial communities influences attraction of mosquitoes to experimental plant infusions. Microb Ecol. 2010, 59: 158-173. 10.1007/s00248-009-9565-1.PubMed CentralPubMedGoogle Scholar
- McCrae AW: Oviposition by African malaria vector mosquitoes II. Effects of site tone, water type and conspecific immatures on target selection by freshwater Anopheles gambiae Giles, sensu lato. Ann Trop Med Parasitol. 1984, 78: 307-318.PubMedGoogle Scholar
- Kennedy J: On water-finding and oviposition by captive mosquitoes. Bull Entomol Res. 1942, 32: 279-301. 10.1017/S0007485300017235.Google Scholar
- Gu W, Regens JL, Beier JC, Novak RJ: Source reduction of mosquito larval habitats has unexpected consequences on malaria transmission. Proc Natl Acad Sci USA. 2006, 103: 17560-17563. 10.1073/pnas.0608452103.PubMed CentralPubMedGoogle Scholar
- Gouagna LC, Rakotondranary M, Boyer S, Lemperiere G, Dehecq JS, Fontenille D: Abiotic and biotic factors associated with the presence of Anopheles arabiensis immatures and their abundance in naturally occurring and manmade aquatic habitats. Parasit Vectors. 2012, 5: 96-10.1186/1756-3305-5-96.PubMed CentralPubMedGoogle Scholar
- Munga S, Vulule J, Kweka EJ: Response of Anopheles gambiae s.l. (Diptera: Culicidae) to larval habitat age in western Kenya highlands. Parasit Vectors. 2013, 6: 13-10.1186/1756-3305-6-13.PubMed CentralPubMedGoogle Scholar
- Kweka EJ, Zhou G, Munga S, Lee MC, Atieli HE, Nyindo M, Githeko AK, Yan G: Anopheline larval habitats seasonality and species distribution: a prerequisite for effective targeted larval habitats control programmes. PLoS One. 2012, 7: e52084-10.1371/journal.pone.0052084.PubMed CentralPubMedGoogle Scholar
- Sumba LA, Guda TO, Deng AL, Hassanali A, Beier JC, Knols BGJ: Mediation of oviposition site selection in the African malaria mosquito Anopheles gambiae (Diptera: Culicidae) by semiochemicals of microbial origin. Int J Trop Insect Sci. 2004, 24: 260-265.Google Scholar
- Sumba LA, Ogbunugafor CB, Deng AL, Hassanali A: Regulation of oviposition in Anopheles gambiae s.s.: role of inter- and intra-specific signals. J Chem Ecol. 2008, 34: 1430-1436. 10.1007/s10886-008-9549-5.PubMedGoogle Scholar
- Huang J, Walker ED, Vulule J, Miller JR: The influence of darkness and visual contrast on oviposition by Anopheles gambiae in moist and dry substrates. Physiol Entomol. 2001, 32: 34-40.Google Scholar
- Huang J, Walker ED, Giroux PY, Vulule J, Miller JR: Ovipositional site selection by Anopheles gambiae: influences of substrate moisture and texture. Med Vet Entomol. 2005, 19: 442-450. 10.1111/j.1365-2915.2005.00588.x.PubMedGoogle Scholar
- Huang J, Walker ED, Otienoburu PE, Amimo F, Vulule J, Miller JR: Laboratory tests of oviposition by the African malaria mosquito, Anopheles gambiae, on dark soil as influenced by presence or absence of vegetation. Malar J. 2006, 5: 88-10.1186/1475-2875-5-88.PubMed CentralPubMedGoogle Scholar
- Kweka EJ, Owino EA, Mwang’onde BJ, Mahande AM, Nyindo M, Mosha F: The role of cow urine in the oviposition site preference of culicine and Anopheles mosquitoes. Parasit Vectors. 2011, 4: 184-10.1186/1756-3305-4-184.PubMed CentralPubMedGoogle Scholar
- Lindh JM, Kannaste A, Knols BG, Faye I, Borg-Karlson AK: Oviposition responses of Anopheles gambiae s.s. (Diptera: Culicidae) and identification of volatiles from bacteria-containing solutions. J Med Entomol. 2008, 45: 1039-1049. 10.1603/0022-2585(2008)45[1039:OROAGS]2.0.CO;2.PubMedGoogle Scholar
- Balestrino F, Soliban SM, Gilles J, Oliva C, Benedict MQ: Ovipositional behavior in the context of mass rearing of Anopheles arabiensis. J Am Mosq Control Assoc. 2010, 26: 365-372. 10.2987/10-6008.1.PubMedGoogle Scholar
- Paaijmans KP, Takken W, Githeko AK, Jacobs AF: The effect of water turbidity on the near-surface water temperature of larval habitats of the malaria mosquito Anopheles gambiae. Int J Biometeorol. 2008, 52: 747-753. 10.1007/s00484-008-0167-2.PubMedGoogle Scholar
- Ye-Ebiyo Y, Pollack RJ, Kiszewski A, Spielman A: Enhancement of development of larval Anopheles arabiensis by proximity to flowering maize (Zea mays) in turbid water and when crowded. Am J Trop Med Hyg. 2003, 68: 748-752.PubMedGoogle Scholar
- Okal MN, Francis B, Herrera-Varela M, Fillinger U, Lindsay SW: Water vapour is a pre-oviposition attractant for the malaria vector Anopheles gambiae sensu stricto. Malar J. 2013, 12: 365-10.1186/1475-2875-12-365.PubMed CentralPubMedGoogle Scholar
- Huang J, Miller JR, Chen SC, Vulule JM, Walker ED: Anopheles gambiae (Diptera: Culicidae) oviposition in response to agarose media and cultured bacterial volatiles. J Med Entomol. 2006, 43: 498-504. 10.1603/0022-2585(2006)43[498:AGDCOI]2.0.CO;2.PubMedGoogle Scholar
- Blackwell A, Johnson SN: Electrophysiological investigation of larval water and potential oviposition chemo-attractants for Anopheles gambiae s.s. Ann Trop Med Parasitol. 2000, 94: 389-398.PubMedGoogle Scholar
- Rinker DC, Pitts RJ, Zhou X, Suh E, Rokas A, Zwiebel LJ: Blood meal-induced changes to antennal transcriptome profiles reveal shifts in odor sensitivities in Anopheles gambiae. Proc Natl Acad Sci USA. 2013, 110: 8260-8265. 10.1073/pnas.1302562110.PubMed CentralPubMedGoogle Scholar
- Haeger J, Provost M: The laboratory colonization of Opifex. Bull World Health Organ. 1964, 31: 451-452.PubMed CentralPubMedGoogle Scholar
- Rasgon JL: Wolbachia induces male-specific mortality in the mosquito Culex pipiens (LIN strain). PLoS One. 2012, 7: e30381-10.1371/journal.pone.0030381.PubMed CentralPubMedGoogle Scholar
- Nguyen TT, Su T, Mulla MS: Bacteria and mosquito abundance in microcosms enriched with organic matter and treated with a Bacillus thuringiensis subsp. israelensis formulation. J Vector Ecol. 1999, 24: 191-201.PubMedGoogle Scholar
- Jackson BT, Paulson SL, Youngman RR, Scheffel SL, Belinda H: Oviposition preferences of Culex restuans and Culex pipiens (Diptera: Culicidae) for selected infusions in oviposition traps and gravid traps. J Am Mosq Control Assoc. 2005, 21: 360-365. 10.2987/8756-971X(2006)21[360:OPOCRA]2.0.CO;2.PubMedGoogle Scholar
- McPhatter LP, Debboun M: Attractiveness of botanical infusions to ovipositing Culex quinquefasciatus, Cx. nigripalpus, and Cx. erraticus in San Antonio, Texas. J Am Mosq Control Assoc. 2009, 25: 508-510. 10.2987/09-5930.1.PubMedGoogle Scholar
- Silver JB: Mosquito Ecology: Field Sampling Methods. 2008, London: SpringerGoogle Scholar
- Minakawa N, Dida GO, Sonye GO, Futami K, Njenga SM: Malaria vectors in Lake Victoria and adjacent habitats in Western Kenya. PLoS One. 2012, 7: e32725-10.1371/journal.pone.0032725.PubMed CentralPubMedGoogle Scholar
- Gillies MT: A modified technique for the age-grading of populations of Anopheles gambiae. Ann Trop Med Parasitol. 1958, 52: 261-273.PubMedGoogle Scholar
- Lyimo EO, Takken W: Effects of adult body size on fecundity and the pre-gravid rate of Anopheles gambiae females in Tanzania. Med Vet Entomol. 1993, 7: 328-332. 10.1111/j.1365-2915.1993.tb00700.x.PubMedGoogle Scholar
- Fillinger U, Knols BG, Becker N: Efficacy and efficiency of new Bacillus thuringiensis var israelensis and Bacillus sphaericus formulations against Afrotropical anophelines in Western Kenya. Trop Med Int Health. 2003, 8: 37-47. 10.1046/j.1365-3156.2003.00979.x.PubMedGoogle Scholar
- Brown RB: Soil texture.http://edis.ifas.ufl.edu/pdffiles/SS/SS16900.pdf,
- Whiting D, Card A, Wilson C, Reeder J: Estimating soil texture: sand, silt or clay?.http://www.ext.colostate.edu/mg/gardennotes/214.html,
- Gillies MT, Coetzee M: A supplement to the anophelinae of Africa South of the Sahara (Afrotropical Region). 1987, Johannesburg: South African Institute for Medical ResearchGoogle Scholar
- Scott JA, Brogdon WG, Collins FH: Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg. 1993, 49: 520-529.PubMedGoogle Scholar
- Knols BG, Njiru BN, Mathenge EM, Mukabana WR, Beier JC, Killeen GF: MalariaSphere: a greenhouse-enclosed simulation of a natural Anopheles gambiae (Diptera: Culicidae) ecosystem in western Kenya. Malar J. 2002, 1: 19-10.1186/1475-2875-1-19.PubMed CentralPubMedGoogle Scholar
- Ponnusamy L, Boroczky K, Wesson DM, Schal C, Apperson CS: Bacteria stimulate hatching of yellow fever mosquito eggs. PLoS One. 2011, 6: e24409-10.1371/journal.pone.0024409.PubMed CentralPubMedGoogle Scholar
- Ponnusamy L, Xu N, Boroczky K, Wesson DM, Abu Ayyash L, Schal C, Apperson CS: Oviposition responses of the mosquitoes Aedes aegypti and Aedes albopictus to experimental plant infusions in laboratory bioassays. J Chem Ecol. 2010, 36: 709-719. 10.1007/s10886-010-9806-2.PubMed CentralPubMedGoogle Scholar
- Bertani G: Lysogeny at mid-twentieth century: P1, P2 and other experimental systems. J Bacteriol. 2004, 186: 595-600. 10.1128/JB.186.3.595-600.2004.PubMed CentralPubMedGoogle Scholar
- Kaur JS, Lai YL, Giger AD: Learning and memory in the mosquito Aedes aegypti shown by conditioning against oviposition deterrence. Med Vet Entomol. 2003, 17: 457-460. 10.1111/j.1365-2915.2003.00455.x.PubMedGoogle Scholar
- Development Core Team R: R: A language and enviroment for statistical computing. pp. http://www.R-project.org/. Vienna, Austria: R Foundation for statistical Computing; 2011:http://www.R-project.org/
- Colton YM, Chadee DD, Severson DW: Natural skip oviposition of the mosquito Aedes aegypti indicated by codominant genetic markers. Med Vet Entomol. 2003, 17: 195-204. 10.1046/j.1365-2915.2003.00424.x.PubMedGoogle Scholar
- Chen H, Fillinger U, Yan G: Oviposition behavior of female Anopheles gambiae in western Kenya inferred from microsatellite markers. Am J Trop Med Hyg. 2006, 75: 246-250.PubMedGoogle Scholar
- Oxford-Dictionaries: Discriminate [Def.2].http://oxforddictionaries.com/definition/english/discrimination,
- Dethier VG, Barton BL, Smith CN: The designation of chemicals in terms of the responses they elicit from Insects. J Econ Entomol. 1960, 53: 134-136.Google Scholar
- Isoe J, Millar JG, Beehler JW: Bioassays for Culex (Diptera: Culicidae) mosquito oviposition attractants and stimulants. J Med Entomol. 1995, 32: 475-483.PubMedGoogle Scholar
- Palmer R: Classification and ecological status of aquatic ecosystems in the eastern Caprivi, Namibia. 2002, Windhoek: Ministry of Agriculture, Water and Rural DevelopmentGoogle Scholar
- Gerhardt RW: The influence of soil fermentation on oviposition site selection by mosquitoes. Mosq News. 1959, 19: 151-155.Google Scholar
- Cunha A, Almeida A, Coelho FJRC, Gomes NCM, Oliveira V, Santos AL: Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. 2010, Bajadoz: Formatex Research CenterGoogle Scholar
- Sigee DC: Fresh water microbiology: biodiversity and dynamic interactions of microorganisms in the aquatic environment. 2005, England: John Wiley & Sons LtdGoogle Scholar
- Burkett-Cadena ND, Mullen GR: Field comparison of Bermuda-hay infusion to infusions of emergent aquatic vegetation for collecting female mosquitoes. J Am Mosq Control Assoc. 2007, 23: 117-123. 10.2987/8756-971X(2007)23[117:FCOBIT]2.0.CO;2.PubMedGoogle Scholar
- Mboera LEG, Mdira KY, Salum FM, Takken W, Pickett JA: Influence of synthetic oviposition pheromone and volatiles from soakage pits and grass infusions upon oviposition site-selection of Culex mosquitoes in Tanzania. J Chem Ecol. 1999, 25: 1855-1865. 10.1023/A:1020933800364.Google Scholar
- Kramer WL, Mulla MS: Oviposition attractants and repellents of mosquitoes: oviposition responses of Culex mosquitoes to organic infusions. Environ Entomol. 1979, 8: 1111-1117.Google Scholar
- Santana AL, Roque RA, Eiras AE: Characteristics of grass infusions as oviposition attractants to Aedes (Stegomyia) (Diptera: Culicidae). J Med Entomol. 2006, 43: 214-220. 10.1603/0022-2585(2006)043[0214:COGIAO]2.0.CO;2.PubMedGoogle Scholar
- Zhang LY, Lei CL: Evaluation of sticky ovitraps for the surveillance of Aedes (Stegomyia) albopictus (Skuse) and the screening of oviposition attractants from organic infusions. Ann Trop Med Parasitol. 2008, 102: 399-407. 10.1179/136485908X300878.PubMedGoogle Scholar
- Trexler JD, Apperson CS, Schal C: Laboratory and field evaluations of oviposition responses of Aedes albopictus and Aedes triseriatus (Diptera: Culicidae) to oak leaf infusions. J Med Entomol. 1998, 35: 967-976.PubMedGoogle Scholar
- Hazard EI, Mayer MS, Savage KE: Attraction and oviposition stimulation of gravid female mosquitoes by bacteria isolated from hay infusions. Mosq News. 1967, 27: 133-136.Google Scholar
- Ikeshoji T, Saito K, Yano A: Bacterial production of the ovipositional attractants for mosquitoes on fatty acid substrates. Appl Entomol Zool. 1975, 10: 239-242.Google Scholar
- Hasselschwert D, Rockett CL: Bacteria as ovipositional attractant for Aedes Aegypti (Diptera:Culicidae). Great Lakes Entomol. 1988, 21: 163-168.Google Scholar
- Allan SA, Kline DL: Evaluation of organic infusions and synthetic compounds mediating oviposition in Aedes albopictus and Aedes aegypti (Diptera: Culicidae). J Chem Ecol. 1995, 21: 1847-1860. 10.1007/BF02033681.PubMedGoogle Scholar
- Trexler JD, Apperson CS, Gemeno C, Perich MJ, Carlson D, Schal C: Field and laboratory evaluations of potential oviposition attractants for Aedes albopictus (Diptera: Culicidae). J Am Mosq Control Assoc. 2003, 19: 228-234.PubMedGoogle Scholar
- Ruppel RE, Setty KE, Wu M: Decomposition rates of Typha spp. in northern freshwater wetlands over a stream–marsh–peatland gradient. Sci Discipulorum. 2004, 1: 26-37.Google Scholar
- Symes CB: Malaria in Nairobi. East Afr Med J. 1940, 17: 332-355.Google Scholar
- Hancock GLR: Some records of Uganda mosquitoes and oecological associations of their larvae. Bull Soc Entomol Egypte. 1930, 1: 38-56.Google Scholar
- Castro MC, Kanamori S, Kannady K, Mkude S, Killeen GF, Fillinger U: The importance of drains for the larval development of lymphatic filariasis and malaria vectors in Dar es Salaam, United Republic of Tanzania. PLoS Negl Trop Dis. 2010, 4: e693-10.1371/journal.pntd.0000693.PubMed CentralPubMedGoogle Scholar
- Kudom AA, Mensah BA, Agyemang TK: Characterization of mosquito larval habitats and assessment of insecticide-resistance status of Anopheles gambiae senso lato in urban areas in southwestern Ghana. J Vector Ecol. 2012, 37: 77-82. 10.1111/j.1948-7134.2012.00202.x.PubMedGoogle Scholar
- Awolola TS, Oduola AO, Obansa JB, Chukwurar NJ, Unyimadu JP: Anopheles gambiae s.s. breeding in polluted water bodies in urban Lagos, southwestern Nigeria. J Vector Borne Dis. 2007, 44: 241-244.PubMedGoogle Scholar
- Seenivasagan T, Sharma KR, Ganesan K, Prakash S: Electrophysiological, flight orientation and oviposition responses of three species of mosquito vectors to hexadecyl pentanoate: residual oviposition repellent activity. J Med Entomol. 2010, 47: 329-337. 10.1603/ME09130.PubMedGoogle Scholar
- Siriporn P, Mayura S: The effects of herbal essential oils on the oviposition-deterrent and ovicidal activities of Aedes aegypti (Linn.), Anopheles dirus (Peyton and Harrison) and Culex quinquefasciatus (Say). Trop Biomed. 2012, 29: 138-150.PubMedGoogle Scholar
- Seenivasagan T, Sharma KR, Prakash S: Electroantennogram, flight orientation and oviposition responses of Anopheles stephensi and Aedes aegypti to a fatty acid ester-propyl octadecanoate. Acta Trop. 2012, 124: 54-61. 10.1016/j.actatropica.2012.06.009.PubMedGoogle Scholar
- Tennyson S, Ravindran KJ, Eapen A, William SJ: Effect of Ageratum houstonianum Mill. (Asteraceae) leaf extracts on the oviposition activity of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res. 2012, 111: 2295-2299. 10.1007/s00436-012-3083-7.PubMedGoogle Scholar
- Lewis LF, Clark TB, O’Grady JJ, Christenson DM: Collecting ovigerous Culex pipiens quinquefasciatus Say near favorable resting sites with louvered traps baited with infusions of alfalfa pellets. Mosq News. 1974, 34: 436-439.Google Scholar
- Lampman RL, Novak RJ: Oviposition preferences of Culex pipiens and Culex restuans for infusion-baited traps. J Am Mosq Control Assoc. 1996, 12: 23-32.PubMedGoogle Scholar
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