High prevalence of asymptomatic malaria in south-eastern Bangladesh
© Starzengruber et al.; licensee BioMed Central Ltd. 2014
Received: 9 October 2013
Accepted: 22 December 2013
Published: 9 January 2014
The WHO has reported that RDT and microscopy-confirmed malaria cases have declined in recent years. However, it is still unclear if this reflects a real decrease in incidence in Bangladesh, as particularly the hilly and forested areas of the Chittagong Hill Tract (CHT) Districts report more than 80% of all cases and deaths. surveillance and epidemiological data on malaria from the CHT are limited; existing data report Plasmodium falciparum and Plasmodium vivax as the dominant species.
A cross-sectional survey was conducted in the District of Bandarban, the southernmost of the three Hill Tracts Districts, to collect district-wide malaria prevalence data from one of the regions with the highest malaria endemicity in Bangladesh. A multistage cluster sampling technique was used to collect blood samples from febrile and afebrile participants and malaria microscopy and standardized nested PCR for diagnosis were performed. Demographic data, vital signs and splenomegaly were recorded.
Malaria prevalence across all subdistricts in the monsoon season was 30.7% (95% CI: 28.3-33.2) and 14.2% (95% CI: 12.5-16.2) by PCR and microscopy, respectively. Plasmodium falciparum mono-infections accounted for 58.9%, P. vivax mono-infections for 13.6%, Plasmodium malariae for 1.8%, and Plasmodium ovale for 1.4% of all positive cases. In 24.4% of all cases mixed infections were identified by PCR. The proportion of asymptomatic infections among PCR-confirmed cases was 77.0%, oligosymptomatic and symptomatic cases accounted for only 19.8 and 3.2%, respectively. Significantly (p < 0.01) more asymptomatic cases were recorded among participants older than 15 years as compared to younger participants, whereas prevalence and parasite density were significantly (p < 0.01) higher in patients younger than 15 years. Spleen rate and malaria prevalence in two to nine year olds were 18.6 and 34.6%, respectively. No significant difference in malaria prevalence and parasite density was observed between dry and rainy season.
A large proportion of asymptomatic plasmodial infections was found which likely act as a reservoir of transmission. This has major implications for ongoing malaria control programmes that are based on the treatment of symptomatic patients. These findings highlight the need for new intervention strategies targeting asymptomatic carriers.
The World Health Organization (WHO) estimated 660,000 deaths in 2011 directly attributed to malaria, approximately half of the world’s population being at risk of infection . The disease has re-emerged in several Central Asian countries and in Southeast Asia partly because of relenting malaria control efforts and the emergence of parasite resistance to the most commonly used anti-malarial drugs . In many regions the vectors have become resistant to the main insecticides and cases of artemisinin resistance have been reported from the Greater Mekong subregion [3–6].
Resistance to chloroquine and sulphadoxine/pyrimethamine (S/P) has been reported from Bangladesh  but until now there is no evidence that artemisinin resistance has spread westwards to Bangladesh, which traditionally forms a gateway to the Indian Subcontinent .
In 2004 the Ministry of Public Health and Family Welfare of Bangladesh revised the malaria treatment guidelines, introducing artemisinin-based combination therapy (ACT) in areas with resistance against chloroquine and S/P . However, ACT was not deployed on a major scale until 2007. Despite the introduction and distribution of ACT in the last years the WHO reports a 70% increase in case numbers between 2000 and 2010 in Bangladesh. However, it is difficult to discern the underlying trend in malaria incidence from improved reporting due to continuous improvements in diagnostic facilities . A seemingly contradictory statement was given in the 2012 report where a decrease of 69% in malaria case incidence between 2000 and 2011 has been reported . Due to a shortage of staff in health care facilities and shortcomings in surveillance and information systems there is still a significant lack of data on malaria from this area [9, 10]. Further increasing drug resistance is significantly aggravating the malaria situation and treatment alternatives to currently used anti-malarials are missing [11–14].
In 2007 a rapid diagnostic test (RDT)-based, cross-sectional survey in 13 eastern districts of the country showed malaria to be endemic within the entire study area. Overall prevalence was reported to be approximately 4%, the majority of cases (90.2%) due to Plasmodium falciparum. Plasmodium vivax and mixed infections accounted for only 5.3%and 4.5%, respectively. Highest prevalence rates were reported from the Chittagong Hill Tract (CHT) with up to 15% . Surveys among febrile patients in the region showed a malaria positivity rate of 26% by microscopy (Swoboda et al. personal communication), which increases to 50% when using polymerase chain reaction (PCR), a considerably more sensitive diagnostic tool .
The high sensitivity of PCR allows detecting subpatent infections that are frequently asymptomatic and it has been shown that those undetected infections represent a considerable fraction of overall infections and may therefore act as a reservoir for transmission [17–21]. The aim of this study was therefore to assess prevalence and proportion of asymptomatic P. falciparum infections in the southernmost district of the CHTs.
Study setting and procedure
Two cross-sectional surveys were performed: the first during the rainy season from August to October 2007 and the second during the dry season from December 2007 to February 2008. The study was conducted by a team from the Medical University of Vienna, Austria in collaboration with the International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh (ICDDR, B). All selected communities were visited by members of the study team, prior to sample collection to inform villagers about the ongoing study. Laboratory tests on collected samples and data analysis were carried out at the Malaria Research Initiative Bandarban (MARIB) field research centre in Bandarban town. Written informed consent was obtained from all study participants or their legal representative, the study protocol was approved by the Ethical Review Committee of the International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B).
During the monsoon season 2007 a total of 21 villages in all seven subdistricts were surveyed (Survey I). Eight villages in three subdistricts (Bandarban, Ruma and Rowangchari) were revisited during the second survey period (Survey II). Whenever possible the study team visited the same households as in the previous survey. Household members who declined to participate or had moved away from the area were replaced by their closest neighbouring household.
Data and sample collection
Participants were interviewed and information was recorded on gender, age, ethnicity, occupation, height, weight, history of fever, number of previous episodes of malaria/fever, last date of malaria diagnosis and result of diagnosis, source and drugs used in malaria treatment, and the number of household members. Axillary temperature and pulse were measured in all participants. Three ml of venous blood was collected from participants eight years or older for malaria RDT/microscopy and 100 μl of venous blood transferred on filter paper (903; Schleicher & Schuell, BioScience GmbH, Dassel, Germany) in duplicate. Five drops of blood (one drop of blood for the malaria RDTs, two for microscopy, and two drops of blood for the PCR filter paper) were drawn by finger or heel prick from children below eight years old.
Malaria was diagnosed in the field by RDT and later confirmed by microscopy and PCR.
Rapid diagnostic test
RDTs (FalciVax®, Zephyr Biomedicals, India) based on the detection of P. falciparum-specific histidine-rich protein 2 (HRP2) and P. vivax-specific lactate dehydrogenase (Pv-pLDH) were employed in all participants with malaria-like symptoms [23, 24]. Patients who tested positive were provided with immediate treatment following national guidelines. Febrile patients considered seriously ill were immediately referred to the closest health care facility for further diagnosis and treatment.
Giemsa-stained blood smears were used for microscopic diagnosis of malaria following established standard operating procedures (SOPs). Thick and thin blood films were prepared, stained with Giemsa stain (Merck®, Darmstadt, Germany) and examined under an oil immersion (Olympus microscope CX21, Tokyo, Japan) for parasite positivity and species determination. Declaring a slide positive or negative and initial species diagnosis was based on the examination of 200 fields in thick films. A slide was considered positive when at least one parasite was found. After finding the first parasite another 200 fields were completed to rule out mixed infections. If no parasite was found in 200 oil fields the slide was considered negative. Parasite density was calculated by counting the number of asexual malaria parasites per 200 white blood cells (WBCs) assuming a WBC count of 8,000/μL .
A minimum of 5% of all positive and negative slides were randomly selected for internal quality control.
Polymerase chain reaction (PCR)
PCR was performed to assess the malaria prevalence in both, symptomatic and asymptomatic participants. DNA was isolated from filter papers using a modified chelex-based technique [26, 27]. Nested PCR assays and Plasmodium genus-specific primers rPLU1/rPLU5 were used for the Nest1 amplification and rPLU3/rPLU4 for Nest2 reactions as described previously [28–30]. All genus-specific PCR-positive results were analysed to species level using the following species-specific primers as reported previously: rFAL1/rFAL2 (P. falciparum); rVIV1/rVIV2 (P. vivax); rMAL1/rMAL2 (P. malariae); rOVA1WC/rOVA2WC (Plasmodium ovale curtisi and Plasmodium ovale wallikeri); rOVA1/rOVA2 (P. ovale curtisi); rOVA1var/rOVA2var (P. ovale wallikeri) [28–33].
Symptomatic clinical malaria cases were defined as PCR-positive individuals with documented fever (axillary temperature ≥37.5°C) and reported clinical symptoms consistent with malaria in the previous seven days. Oligo-symptomatic malaria cases were defined as PCR-positive, afebrile (axillary temperature <37.5°C) cases with a reported history of fever or illness in the previous seven days. Asymptomatic malaria cases were defined as PCR-positive cases without measurable fever (axillary temperature <37.5°C), who reported no malaria-related symptoms, and had not received treatment for malaria in the previous seven days .
The spleen rate was determined for children aged 14 years and below. Spleen size was measured followingthe method established by Hackett  and classified either as negative (Hackett grade 0) or positive (Hackett grades 1–5).
Classification of malaria endemicity
The four classes of endemic malaria were defined as following : hypo-endemic malaria: spleen and/or parasite rate in children two to nine years not exceeding 10%; meso-endemic malaria: spleen and/or parasite rate rate in children two to nine years between 11 and 50%; hyperendemic malaria: spleen and/or parasite rate rate in children two to nine years constantly over 50% and a high spleen and/or parasite rate rate in adults (over 25%); holo-endemic malaria: spleen and/or parasite rate rate in children two to nine years constantly over 75%, but a low spleen and/or parasite rate rate in adults.
P < 0.05 was considered significant. Pearson’s Chi-square with Yates’ correction or Fisher’s Exact test as appropriate were used for categorical data, Mann–Whitney U-test was performed for comparing continuous data that did not conform to normal distribution. Student’s t-test was conducted to evaluate differences between quantitative variables that were normally distributed. All analyses were performed using Microsoft Excel® and VassarStats .
Demographic data of the study population
Baseline characteristics of the study population
Total number of individuals 1,874
Summer survey I
Winter survey II
No of individuals
Median age (IQR)
Age groups (years)
Mean weight kg (range)
Mean height cm (range)
None (students, children)
Malaria prevalence and fever in cross-sectional survey I
Malaria prevalence, parasite density in Bandarban District (Survey I)
Parasite density/Age (years)
P. f. (parasites/μl blood1)
P. v. (parasites/μl blood1)
With 188 cases (36.3%, 95% CI: 32.2-40.6) the malaria prevalence in individuals under 15 years was significant higher (p < 0.01) compared to older participants (N = 247, 27.4%, 95% CI: 24.6-30.5). A significantly higher malaria prevalence (p = 0.015) was found in males (N = 220, 34.0%, 95% CI: 30.4-37.8) as compared to females (N = 215, 27.9%, 95% CI: 24.8-31.2).
The overall geometric mean parasite density determined by microscopy was 707 parasites/μl (95% CI: 565–883) for P. falciparum and 345 parasites/μl (95% CI: 246–484) for P. vivax. Parasite densities in individuals younger than 15 years were significantly higher (p < 0.01).
Distribution of symptomatic, oligosymptomatic and asymptomatic PCR/microscopy positive malaria cases during monsoon and dry season
Survey I (monsoon season)
Symptomatic malaria cases
Oligosymtomatic malaria cases
Asymptomatic malaria cases
Survey II (dry season)
Out of 416 households in 223 (53.6%, 95% CI: 48.7-58.5) at least one member of the household tested positive for malaria, in 64 households (15.4%, 95% CI: 12.1-19.3) two people tested positive for malaria and in 56 (13.5%, 95% CI: 10.4-17.2) households three or more individuals tested positive by PCR.
Spleen enlargement as predictor for malaria in children two to nine years old
Winter survey II
Malaria prevalence in three Bandarban subdistricts (Survey II)
Plasmodium falciparum gametocytes were detected by microscopy in four (3.5%, 95% CI: 1.1-9.1) individuals of whom only one also had asexual parasites. Malaria prevalence within all participants in the winter survey (dry season) was not significantly (p = 0.117) lower compared to the summer survey.
No patient testing positive for malaria was febrile at the time of the survey, therefore 103 (88.8%, 95% CI: 81.3-93.7) were classified as asymptomatic and 13 (11.2%, 95% CI: 6.3-18.7) as oligosymptomatic (Table 3).
The geometric mean parasite densities were 487 asexual parasites/μl (95% CI: 281–845) for P. falciparum and 751 (95% CI: 298–1,894) for P. vivax, respectively. No statistically difference (p > 0.05) was found between the parasite rate in summer and winter survey.
From a total of 172 households, in 77 (44.8%, 95% CI: 37.3-52.5) at least one member tested positive for malaria, in 27 (15.7%, 95% CI: 1.4-7.8) households two individuals and in seven (4.1%, 95% CI: 1.8-8.5) households three or more people tested positive for malaria.
In the winter survey the highest malaria prevalence by subdistrict was found in Bandarban Sadar (29.7%, 95% CI: 24.4-35.5) followed by Rowangchhari (27.1%, 95% CI: 18.8-37.3), and the lowest malaria prevalence was detected in Ruma (13.4%, 95% CI: 6.7-24.5).
Out of 97 children aged two to nine years, nine (9.3%, 95% CI: 4.6-17.3) had a palpable spleen and with 30.9% (N = 30; 95% CI: 22.2-41.2) the parasite prevalence by PCR in survey II was again high but not significantly different from survey I (p = 0.584). Malaria-positive PCR and an enlarged spleen at the same time were found in four (4.1%, 95% CI: 1.3-10.8) children two to nine years old. With 9.3% the spleen rate in winter was also lower than in summer (18.6%; p = 0.043). Sensitivity, specificity, PPV and NPV of spleen enlargement for malaria in the winter survey are shown in Table 4.
Malaria is a major public health problem in southeastern Bangladesh and remains one of the most common reasons for hospital admissions during the malaria season . Mapping of high-risk areas is essential for planning health interventions . The primary objective of this investigation was to establish detailed baseline data on the prevalence and distribution of malaria in both symptomatic and asymptomatic carriers of Plasmodium parasites in southeastern Bangladesh.
Three major observations can be deducted from these cross-sectional surveys. First, this study demonstrates that malaria is meso-endemic in the region and is concentrated in rural communities  where the intensity of transmission is largely dependent on environmental variables. Haque et al. have previously reported for this region that environmental factors, such as proximity to forest, household density, and elevation tend to be significantly and positively correlated with malaria risk . The development of immunity is insufficient to prevent the effect of malaria on all age groups . A spleen rate of 18.6% (proportions of splenomegaly >10% are considered to be related to malaria ) and malaria prevalence by microscopy of 14.2% in the rainy and 7.8% in dry season, show that malaria is meso-endemic throughout the year in this part of Bangladesh. The spleen rate and the higher malaria prevalence in children are further indicators of meso-endemicity and suggest that transmission occurs within the villages [35, 38].
Second, malaria prevalence was significantly higher in the northern and eastern subdistricts that are located in the hilly areas bordering Myanmar and Rangamati, another endemic district, whereas endemicity was lower in western and southern subdistricts in or close to the coastal plains, which are more accessible and where access to health care facilities is better. The collected data indicate a level of malaria transmission similar to some African countries, such as Gabon , Somalia  or Mozambique . Previous studies from India and Indonesia also detected similar parasite prevalence rates [42, 43].
Third, a large reservoir of asymptomatic, parasitaemic individuals that are likely to act as a source of infection, was observed in both surveys. The absence of malaria-like symptoms may be an indication of a certain level of immunity in rural communities. Although generally considered to be a typical feature of malaria in highly endemic regions in Africa, a number of studies from Brazil, Peru, Thailand, Cambodia, Myanmar, Vietnam, eastern Indonesia, and Papua New Guinea have reported the existence of asymptomatic malaria infections outside of Africa and in areas with lower endemicity [34, 43–49]. This survey indicates a similar proportion of asymptomatic malaria carriers as previously reported from Vietnam , Indonesia  and Cambodia .
Naturally acquired immunity against P. falciparum, which is believed to build up with long-term exposure to malaria and presents with lower parasite densities and fewer clinical malaria episodes in older children and adults, has been reported from endemic areas in Myanmar, eastern Indonesia and India . In areas with lower transmission intensity, the age at which clinical immunity develops tends to shift to an older age . Malaria prevalence, parasite density and the number of clinical malaria cases were higher in children under 15 years.
As previously reported by Alves et al. , asymptomatic carriers may act as a reservoir for parasites and are a likely source of infection. However, treatment is typically only provided to symptomatic patients as asymptomatic carriers are rarely seen and/or diagnosed at health care facilities. Particularly in times of malaria elimination, asymptomatic malaria carrier with a low parasitaemia will present new challenges for malaria control in the region where malaria diagnosis is mainly based on microscopy and RDT.
Limitations of this study include all potential shortcomings of a point-prevalence study as well as the potential bias arising from the fact that only one district has been surveyed which may not be representative for all 13 malaria endemic districts in Bangladesh. Secondly, there was some overlap with the previously reported cross-sectional survey based on RDTs , which, however, provides far less detailed epidemiological data.
The surveys showed that there are areas in Bangladesh with prevalence rates comparable to those found in malaria-endemic regions of tropical Africa. This study indicates, in accordance with other studies from Southeast Asia, that there is still a substantial proportion of asymptomatic, parasitaemic individuals in Bangladesh that may act as a silent reservoir for malaria transmission. This has major implications for ongoing malaria control programs in Bangladesh that are based on prevention of infection through bed nets and treatment of symptomatic patients. Particularly considering ongoing elimination efforts these findings highlight the need for new intervention strategies targeting all infections, symptomatic as well as asymptomatic, for reducing potential sources of infection and for interrupting the transmission cycle. Based on this study, it is evident that malaria remains an important public health problem in the southeastern part of Bangladesh. Further research is needed to determine the role of asymptomatic individuals for malaria transmission in the area.
We wish to thank all the people who participated in these surveys, the Ministry of Health and Family Welfare, the staff of the Sadar Hospital Bandarban and the members of the Civil Surgeon’s office Bandarban, Bangladesh for their cooperation and assistance as well as the Bangladesh Army for their assistance in the field.
- World Health Organization: World Malaria Report 2012.http://www.who.int/malaria/publications/world_malaria_report_2012/wmr2012_no_profiles.pdf,
- Cui L, Yan G, Sattabongkot J, Cao Y, Chen B, Chen X, Fan Q, Fang Q, Jongwutiwes S, Parker D, Sirichaisinthop J, Kyaw MP, Su XZ, Yang H, Yang Z, Wang B, Xu J, Zheng B, Zhong D, Zhou G: Malaria in the greater Mekong subregion: heterogeneity and complexity. Acta Trop. 2012, 121: 227-239. 10.1016/j.actatropica.2011.02.016.PubMed CentralView ArticlePubMedGoogle Scholar
- Noedl H, Se Y, Schaecher K, Smith BL, Socheat D, Fukuda MM: Artemisinin resistance in Cambodia 1 (ARC1) study consortium: evidence of artemisinin-resistant malaria in western Cambodia. N Engl J Med. 2008, 359: 2619-2620. 10.1056/NEJMc0805011.View ArticlePubMedGoogle Scholar
- Noedl H, Se Y, Sriwichai S, Schaecher K, Teja-Isavadharm P, Smith B, Rutvisuttinunt W, Bethell D, Surasri S, Fukuda MM, Socheat D, Chan Thap L: Artemisinin resistance in Cambodia: a clinical trial designed to address an emerging problem in Southeast Asia. Clin Infect Dis. 2010, 51: e82-e89. 10.1086/657120.View ArticlePubMedGoogle Scholar
- Noedl H, Socheat D, Satimai W: Artemisinin-resistant malaria in Asia. N Engl J Med. 2009, 361: 540-541. 10.1056/NEJMc0900231.View ArticlePubMedGoogle Scholar
- Phyo AP, Nkhoma S, Stepniewska K, Ashley EA, Nair S, McGready R, Ler Moo C, Al-Saai S, Dondorp AM, Lwin KM, Singhasivanon P, Day NP, White NJ, Anderson TJ, Nosten F: Emergence of artemisinin-resistant malaria on the Western border of Thailand: a longitudinal study. Lancet. 2012, 379: 1960-1966. 10.1016/S0140-6736(12)60484-X.PubMed CentralView ArticlePubMedGoogle Scholar
- Starzengruber P, Swoboda P, Fuehrer HP, Khan WA, Hofecker V, Siedl A, Fally M, Graf O, Teja-Isavadharm P, Haque R, Ringwald P, Noedl H: Current status of artemisinin-resistant falciparum malaria in South Asia: a randomized controlled artesunate monotherapy trial in Bangladesh. PLoS One. 2012, 7: e52236-10.1371/journal.pone.0052236.PubMed CentralView ArticlePubMedGoogle Scholar
- World Health Organization: World Malaria Report 2011.http://www.who.int/malaria/world_malaria_report_2011/WMR2011_noprofiles_lowres.pdf,
- Bangali AM, Mahmood MAH, Rahman M: The malaria situation in Bangladesh. Mekong Malaria Forum. 2000, 6: 20-24.Google Scholar
- Faiz MA, Yunus EB, Rahman MR, Hossain MA, Pang LW, Rahman ME, Bhuiyan SN: Failure of national guidelines to diagnose uncomplicated malaria in Bangladesh. Am J Trop Med Hyg. 2002, 67: 396-399.PubMedGoogle Scholar
- Thriemer K, Haque R, Wagatsuma Y, Salam MA, Akther S, Attlmayr B, Fukuda M, Schaecher K, Miller RS, Noedl H: Therapeutic efficacy of quinine plus sulfadoxine-pyrimethamine for the treatment of uncomplicated falciparum malaria in Bangladesh. Am J Trop Med Hyg. 2006, 75: 645-649.PubMedGoogle Scholar
- Haque R, Thriemer K, Wang Z, Sato K, Wagatsuma Y, Salam MA, Akther S, Akter J, Fukuda M, Miller RS, Noedl H: Therapeutic efficacy of artemether-lumefantrine for the treatment of uncomplicated Plasmodium falciparum malaria in Bangladesh. Am J Trop Med Hyg. 2007, 76: 39-41.PubMedGoogle Scholar
- Attlmayr B, Thriemer K, Haque R, Wagatsuma Y, Abdus Salam M, Akhter S, Fukuda M, Schaecher K, Miller RS, Noedl H: In vitro antimalarial drug resistance in Southeastern Bangladesh. Wien Klin Wochenschr. 2006, 118: 58-61.View ArticlePubMedGoogle Scholar
- Noedl H, Faiz MA, Yunus EB, Rahman MR, Hossain MA, Samad R, Miller RS, Pang LW, Wongsrichanalai C: Drug-resistant malaria in Bangladesh: an in vitro assessment. Am J Trop Med Hyg. 2003, 68: 140-142.PubMedGoogle Scholar
- Haque U, Ahmed SM, Hossain S, Huda M, Hossain A, Alam MS, Mondal D, Khan WA, Khalequzzaman M, Haque R: Malaria prevalence in endemic districts of Bangladesh. PLoS One. 2009, 4: e6737-10.1371/journal.pone.0006737.PubMed CentralView ArticlePubMedGoogle Scholar
- Fuehrer HP, Starzengruber P, Swoboda P, Khan WA, Matt J, Ley B, Thriemer K, Haque R, Yunus EB, Hossain SM, Walochnik J, Noedl H: Indigenous Plasmodium ovale malaria in Bangladesh. Am J Trop Med Hyg. 2010, 83: 75-78.PubMed CentralView ArticlePubMedGoogle Scholar
- Ouédraogo AL, Bousema T, Schneider P, De Vlas SJ, Ilboudo-Sanogo E, Cuzin-Ouattara N, Nébié I, Roeffen W, Verhave JP, Luty AJ, Sauerwein R: Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission. PLoS One. 2009, 4: e8410-10.1371/journal.pone.0008410.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharp BL, Kleinschmidt I, Streat E, Maharaj R, Barnes KI, Durrheim DN, Ridl FC, Morris N, Seocharan I, Kunene S, LA Grange JJ, Mthembu JD, Maartens F, Martin CL, Barreto A: Seven years of regional malaria control collaboration-Mozambique, South Africa, and Swaziland. Am J Trop Med Hyg. 2007, 76: 42-47.PubMed CentralPubMedGoogle Scholar
- Mabunda S, Aponte JJ, Tiago A, Alonso P: A country-wide malaria survey in Mozambique. II. Malaria attributable proportion of fever and establishment of malaria case definition in children across different epidemiological settings. Malar J. 2009, 8: 74-10.1186/1475-2875-8-74.PubMed CentralView ArticlePubMedGoogle Scholar
- Vafa M, Troye-Blomberg M, Anchang J, Garcia A, Migot-Nabias F: Multiplicity of Plasmodium falciparum infection in asymptomatic children in Senegal: relation to transmission, age and erythrocyte variants. Malar J. 2008, 7: 17-10.1186/1475-2875-7-17.PubMed CentralView ArticlePubMedGoogle Scholar
- Dal-Bianco MP, Köster KB, Kombila UD, Kun JF, Grobusch MP, Ngoma GM, Matsiegui PB, Supan C, Salazar CL, Missinou MA, Issifou S, Lell B, Kremsner P: High prevalence of asymptomatic Plasmodium falciparum infection in Gabonese adults. Am J Trop Med Hyg. 2007, 7: 939-942.Google Scholar
- United Nations: Series F No.98. Designing Household Survey Samples: Practical Guidelines. Studies in Methods. 2008, New YorkGoogle Scholar
- Moody A: Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev. 2002, 15: 66-78. 10.1128/CMR.15.1.66-78.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Meena M, Joshi D, Joshi R, Sridhar S, Waghdhare S, Gangane N, Kalantri SP: Accuracy of a multispecies rapid diagnostic test kit for detection of malarial parasite at the point of care in a low endemicity region. Trans R Soc Trop Med Hyg. 2009, 103: 1237-1244. 10.1016/j.trstmh.2009.04.011.View ArticlePubMedGoogle Scholar
- World Health Organization: Basic malaria microscopy. 1991, Geneva: WHO, Reprint 1995)Google Scholar
- Chaorattanakawee S, Natalang O, Hananantachai H, Nacher M, Brockman A, Krudsood S, Looareesuwan S, Patarapotikul J: Storage duration and polymerase chain reaction detection of Plasmodium falciparum from blood spots on filter paper. Am J Trop Med Hyg. 2003, 69: 42-44.PubMedGoogle Scholar
- Bereczky S, Mårtensson A, Gil JP, Färnert A: Rapid DNAextraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum. Am J Trop Med Hyg. 2005, 72: 249-251.PubMedGoogle Scholar
- Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, Do Rosario VE, Thaithong S, Brown KN: High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol. 1993, 61: 315-320. 10.1016/0166-6851(93)90077-B.View ArticlePubMedGoogle Scholar
- Snounou G, Singh B: Nested PCR analysis of Plasmodium parasites. Methods Mol Med. 2002, 72: 189-203.PubMedGoogle Scholar
- Singh B, Bobogare A, Cox-Singh J, Snounou G, Abdullah MS, Rahman HA: A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. Am J Trop Med Hyg. 1999, 60: 687-692.PubMedGoogle Scholar
- Fuehrer HP, Fally MA, Habler VE, Starzengruber P, Swoboda P, Noedl H: Novel nested direct PCR technique for malaria diagnosis using filter paper samples. J Clin Microbiol. 2011, 49: 1628-1630. 10.1128/JCM.01792-10.PubMed CentralView ArticlePubMedGoogle Scholar
- Calderaro A, Piccolo G, Perandin F, Gorrini C, Peruzzi S, Zuelli C, Ricci L, Manca N, Dettori G, Chezzi C, Snounou G: Genetic polymorphisms influence Plasmodium ovale PCR detection accuracy. J Clin Microbiol. 2007, 45: 1624-1627. 10.1128/JCM.02316-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Fuehrer HP, Stadler MT, Buczolich K, Bloeschl I, Noedl H: Two techniques for simultaneous identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by use of the small-subunit rRNA gene. J Clin Microbiol. 2012, 50: 4100-4102. 10.1128/JCM.02180-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Laishram DD, Sutton PL, Nanda N, Sharma VL, Sobti RC, Carlton JM, Joshi H: The complexities of malaria disease manifestations with a focus on asymptomatic malaria. Malar J. 2012, 11: 29-10.1186/1475-2875-11-29.PubMed CentralView ArticlePubMedGoogle Scholar
- Gilles HM, Warrell DA: Bruce-Chwatt’s Essential Malariology. 1999, Oxford: Oxford University Press, 368pp-3Google Scholar
- VassarStats: Website for Statistical Computation. -http://vassarstats.net,
- Dev V, Phookan S, Sharma VP, Anand SP: Physiographic and entomologic risk factors of malaria in Assam, India. Am J Trop Med Hyg. 2004, 71: 451-456.PubMedGoogle Scholar
- World Health Organization: Global malaria control and elimination: a report of technical review. 2008, Geneve, SwitzerlandGoogle Scholar
- Haque U, Soares Magalhães RJ, Mitra D, Kolivras KN, Schmidt WP, Haque R, Glass GE: The role of age, ethnicity and environmental factors in modulating malaria risk in Rajasthali, Bangladesh. Malar J. 2011, 10: 367-10.1186/1475-2875-10-367.PubMed CentralView ArticlePubMedGoogle Scholar
- Noor AM, Moloney G, Borle M, Fegan GW, Shewchuk T, Snow RW: The use of mosquito nets and the prevalence of Plasmodium falciparum infection in rural South Central Somalia. PLoS One. 2008, 3: e2081-10.1371/journal.pone.0002081.PubMed CentralView ArticlePubMedGoogle Scholar
- Mayor A, Aponte JJ, Fogg C, Saúte F, Greenwood B, Dgedge M, Menendez C, Alonso PL: The epidemiology of malaria in adults in a rural area of southern Mozambique. Malar J. 2007, 6: 3-10.1186/1475-2875-6-3.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharma SK, Tyagi PK, Padhan K, Upadhyay AK, Haque MA, Nanda N, Joshi H, Biswas S, Adak T, Das BS, Chauhan VS, Chitnis CE, Subbarao SK: Epidemiology of malaria transmission in forest and plain ecotype villages in Sundargarh District, Orissa, India. Trans R Soc Trop Med Hyg. 2006, 100: 917-925. 10.1016/j.trstmh.2006.01.007.View ArticlePubMedGoogle Scholar
- Karyana M, Burdarm L, Yeung S, Kenangalem E, Wariker N, Maristela R, Umana KG, Vemuri R, Okoseray MJ, Penttinen PM, Ebsworth P, Sugiarto P, Anstey NM, Tjitra E, Price RN: Malaria morbidity in Papua Indonesia, an area with multidrug resistant Plasmodium vivax and Plasmodium falciparum. Malar J. 2008, 7: 148-10.1186/1475-2875-7-148.PubMed CentralView ArticlePubMedGoogle Scholar
- Erhart A, Ngo DT, Phan VK, Ta TT, Van Overmeir C, Speybroeck N, Obsomer V, Le XH, Le KT, Coosemans M, D’alessandro U: Epidemiology of forest malaria in central Vietnam: a large scale cross-sectional survey. Malar J. 2005, 4: 58-10.1186/1475-2875-4-58.PubMed CentralView ArticlePubMedGoogle Scholar
- Incardona S, Vong S, Chiv L, Lim P, Nhem S, Sem R, Khim N, Doung S, Mercereau-Puijalon O, Fandeur T: Large-scale malaria survey in Cambodia: novel insights on species distribution and risk factors. Malar J. 2007, 6: 37-10.1186/1475-2875-6-37.PubMed CentralView ArticlePubMedGoogle Scholar
- Alves FP, Durlacher RR, Menezes MJ, Krieger H, Silva LH, Camargo EP: High prevalence of asymptomatic Plasmodium vivax and Plasmodium falciparum infections in native Amazonian populations. Am J Trop Med Hyg. 2002, 66: 641-648.PubMedGoogle Scholar
- Roshanravan B, Kari E, Gilman RH, Cabrera L, Lee E, Metcalfe J, Calderon M, Lescano AG, Montenegro SH, Calampa C, Vinetz JM: Endemic malaria in the Peruvian Amazon region of Iquitos. Am J Trop Med Hyg. 2003, 69: 45-52.PubMedGoogle Scholar
- Thang ND, Erhart A, Speybroeck N, Hung Le X, Thuan Le K, Hung CT, Ky PV, Coosemans M, D’Alessandro U: Malaria in central Vietnam: analysis of risk factors by multivariate analysis and classification tree models. Malar J. 2008, 7: 28-10.1186/1475-2875-7-28.PubMed CentralView ArticlePubMedGoogle Scholar
- Brown T, Smith LS, Oo EK, Shawng K, Lee TJ, Sullivan D, Beyrer C, Richards AK: Molecular surveillance for drug-resistant Plasmodium falciparum in clinical and subclinical populations from three border regions of Burma/Myanmar: cross-sectional data and a systematic review of resistance studies. Malar J. 2012, 11: 333-10.1186/1475-2875-11-333.PubMed CentralView ArticlePubMedGoogle Scholar
- Thang ND, Erhart A, Hung Le X, Thuan Le K, Xa NX, Thanh NN, Ky PV, Coosemans M, Speybroeck N, D’Alessandro U: Rapid decrease of malaria morbidity following the introduction of community-based monitoring in a rural area of central Vietnam. Malar J. 2009, 8: 3-10.1186/1475-2875-8-3.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.