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Spatial and temporal epidemiology of clinical malaria in Cambodia 2004–2013
Malaria Journalvolume 13, Article number: 385 (2014)
Artemisinin-resistant Plasmodium falciparum malaria has recently been identified on the Thailand-Cambodia border and more recently in parts of Thailand, Myanmar and Vietnam. There is concern that if this resistance were to spread, it would severely hamper malaria control and elimination efforts worldwide. Efforts are currently underway to intensify malaria control activities and ultimately eliminate malaria from Cambodia. To support these efforts, it is crucial to have a detailed picture of disease burden and its major determinants over time.
An analysis of spatial and temporal data on clinical malaria in Cambodia collected by the National Centre for Parasitology, Entomology and Malaria Control (CNM) and the Department of Planning and Health Information, Ministry of Health Cambodia from 2004 to 2013 is presented.
There has been a marked decrease of 81% in annual cases due to P. falciparum since 2009 coinciding with a rapid scale-up in village malaria workers (VMWs) and insecticide-treated bed nets (ITNs). Concurrently, the number of cases with Plasmodium vivax has greatly increased. It is estimated that there were around 112,000 total cases in 2012, 2.8 times greater than the WHO estimate for that year, and 68,000 in 2013 (an annual parasite incidence (API) of 4.6/1000). With the scale-up of VMWs, numbers of patients presenting to government facilities did not fall and it appears likely that those who saw VMWs had previously accessed healthcare in the private sector. Malaria mortality has decreased, particularly in areas with VMWs. There has been a marked decrease in cases in parts of western Cambodia, especially in Pailin and Battambang Provinces. In the northeast, the fall in malaria burden has been more modest, this area having the highest API in 2013.
The clinical burden of falciparum malaria in most areas of Cambodia has greatly decreased from 2009 to 2013, associated with roll-out of ITNs and VMWs. Numbers of cases with P. vivax have increased. Possible reasons for these trends are discussed and areas requiring further study are highlighted. Although malaria surveillance data are prone to collection bias and tend to underestimate disease burden, the finding of similar trends in two independent datasets in this study greatly increased the robustness of the findings.
Epidemiological data on malaria over time are essential to planning of malaria control and elimination. In Cambodia, the control and elimination of malaria has garnered much attention due the recent discovery of artemisinin resistance in Pailin Province on the Thai border . This area has historically been the source of resistance to other anti-malarial drugs and if artemisinin resistance were also to spread it would be a major threat to malaria control and elimination efforts worldwide [2, 3]. Resistance has since been identified in elsewhere in Cambodia and parts of Thailand , Myanmar  and Vietnam . Mathematical modelling has been employed to predict the most effective interventions to try to eliminate artemisinin-resistant malaria parasites [7, 8]. The modelling predictions are heavily dependent on accurate information on baseline malaria epidemiology.
Since 1992, the Department of Planning and Health Information has used a standardized system for collecting monthly data on malaria cases across the whole of Cambodia into a centralized database, the Health Information System (HIS). This includes clinical cases in each province who attend public sector health facilities. Since 2004, the National Centre for Parasitology, Entomology and Malaria Control (CNM) has rolled out a system of Village Malaria Workers (VMWs) across the areas of the country with highest malaria prevalence. These provide diagnosis and treatment at the village level and report their activities through the Malaria Information System (MIS) on cases diagnosed and treated to CNM. Between these two sources, HIS and MIS, a detailed picture of malaria in Cambodia over time can be gleaned.
This study analysed data collected through HIS and MIS from 2004 to 2013 to produce a picture of the spatial and temporal epidemiology of malaria in Cambodia.
Data were extracted from the HIS and MIS databases and CNM annual reports on numbers of malaria cases and malaria control activities from January 2004 to December 2013. For clarity in Figures throughout this manuscript, the MIS data are referred to as ‘VMW’. Data on population and average rainfall in Cambodia were also collected [9, 10]. These were analysed using Microsoft Excel 2013 and GraphPad Prism Version 6.04 and maps produced using ARC GIS 10.2.
Statistical analyses were performed using GraphPad Prism Version 6.04. Correlations were examined using Spearman R and significance was set at the 5% level.
Health information system
From 2004–2013, 1,667,188 people tested for malaria in Cambodia were recorded by the HIS system. Of these, 496,070 (29.8%) were positive, 385,045 (77.6%) with Plasmodium falciparum and 139,254 (28.1%) with Plasmodium vivax. 28,229 (5.7%) had mixed infection. 666,019 people were recorded as treated for malaria. Thus at least 169,949/666,019 (25.5%) of those treated for malaria did not have parasitological confirmation of malaria. During this period, there were 2085 (0.5%) recorded deaths from malaria. The yearly average number of confirmed malaria cases in the HIS was 49,607. Diagnosis was performed using microscopy or a rapid diagnostic test (RDT). The proportion of HIS recorded patients diagnosed by microscopy fell from 52% in 2007 to 14% in 2013.
The numbers of cases of both falciparum and vivax malaria were highly seasonal with a broad peak and the highest monthly numbers from June to January, the time of highest rainfall (Figure 1). In most years, the peaks in cases with falciparum and vivax malaria were in different months. The month with the highest number of cases varied from June to January with two peaks for both species in some years.
Overall from 2004 to 2013, there has been a marked decrease in P. falciparum and an increase in P. vivax (Figure 2).
Much of this decrease was from 2011–2013 during which the total number of cases with either species fell by 59% (Figure 2A). For P. falciparum, from 2004 to 2009 there was no clear overall trend in the annual number of cases. Since 2009, there has been a marked and steady decrease in annual cases of 81% (p = 0.006). The number of cases with P. vivax remained stable from 2004 to 2008. It then increased from 2008 to 2011 (p = 0.01), the total number being 4.9 times greater in 2011 than in 2008. This was followed by a 50% decline in P. vivax cases from 2011 to 2013. The proportion of cases due to P. vivax was 12% in 2004 and this remained steady until it began to rise from 23% in 2009 to 67% in 2013.From 2004 to 2011 the number of people tested and proportion of tests positive for malaria did not change (Figure 2B, p = 0.11), indicating that the overall trends in cases detected were not an artefact due to changing surveillance efforts. Since 2011, the proportion of tests which were positive has steadily fallen, suggesting a relative intensification of malaria testing and consistent with a true decline in incidence of clinical malaria.
From 2004 to 2013, the number of deaths from malaria and % mortality from P. falciparum decreased, p = 0.0003 and p < 0.0001, respectively (Figure 3). As the parasite species in fatal cases was not recorded, it was assumed all deaths were due to P. falciparum.
Almost all ODs had cases of malaria in 2004 and 2013 (Figures 4, and 5 for reference). 3/75 ODs had no cases of malaria in 2004 and 3/77 in 2013. The spatial distribution of malaria cases was similar for P. falciparum and P. vivax. In 2013, the highest transmission intensity for both species was in the north and northeast of the country, some areas of which remain heavily forested. The four operational districts (ODs) with the highest transmission for P. falciparum in descending order were Rattanakiri (annual parasite incidence (API) 13.6/1000), Steung Treng (API 10.2/1000), Kratie (API 9.1/1000) and Preah Vihear (API 4.4/1000). The four ODs with the highest transmission for P. vivax in 2013 were Preah Vihear (API 17.1/1000), Rattanakiri (API 11.3/1000), Samrong (API 10.1/1000) and Steung Treng (API 8.8/1000). Although the four provinces in the northeast of Cambodia (Mondulkiri, Rattanakiri, Steung Treng and Kratie) had only 3% of the population, they had 43% of the falciparum malaria and 25% of the vivax malaria cases in 2013
There were several major trends. In most ODs, P. falciparum decreased from 2004 to 2013 and P. vivax increased, although there were some exceptions. From 2004 to 2013 P. falciparum remained widespread across Cambodia with low and unstable transmission in the southeast. P. vivax transmission was much less stable, with low and unstable transmission in many areas, particularly in a band from the southeast to the northwest of the country.The changes in numbers of cases from 2004 to 2013 for each OD are presented in Figure 6.
In the west of Cambodia (Pailin, Battambang, Banteay Meanchey, Pursat, Siem Reap and Oddar Meanchey provinces) there was a marked and steady decrease in P. falciparum from 2007 onwards (Figure 7A). This decrease was most marked in Pailin (Figure 7B), the OD with the highest transmission intensity from 2004–2006, and to a lesser degree in the surrounding province of Battambang (Figure 7C) with historically much lower transmission than Pailin. Also in Pailin, there was no increase in cases of P. vivax from 2008 to 2011, unlike most other malaria endemic areas of the country. In parts of the west of Cambodia, the decrease in P. falciparum was less marked, for example Pursat Province (Figure 7D). The ODs along the border with Thailand (in the North and West), with the exception of Battambang and Pailin, did not experience a sustained decrease in transmission until 2012 (Figure 7E). This was also the case in the provinces of Kampot and Kampong Speu in the South (Figure 7F). There was an increase in P. falciparum malaria in the Northeast of Cambodia from 2007–2010 (Figure 8A, Rattanakiri from 2007–2010 (Figure 8B) and Kratie (Figure 8C) and Steung Treng (Figure 8D) from 2007–2009 with no increase in Sen Monorom (Figure 8E). Although cases in the Northeast have since decreased by 58%, it remains the area with the highest transmission. The seasonality in monthly numbers of cases was also much more prominent in the North and East than in the West. In Preah Vihear Province in the North (Figure 8F), the relative increase in cases from 2008–2011 was particularly marked. P. vivax transmission was most stable in the ODs along the borders with Thailand (Figure 7E), Vietnam (Figure 8G) and Laos (Figure 8H).
Malaria control activities
During the period of the data collection, the sale of artemisinin combination therapy (ACT) for treatment of malaria increased, insecticide-treated bed net distribution was scaled up and long-lasting insecticide treated bed nets were introduced in 2007 (Figure 9). Detailed data on actual numbers of ACT taken and bed net usage were not included in this study, and the figures for sales and distribution were used as an approximate surrogate. There were no clear relationships between the number of courses of ACT distributed and changes in the number of malaria cases reported through HIS.
Village malaria workers
The number of VMWs has gradually increased in Cambodia since their official introduction in 2004 (Figure 10), following small-scale pilots from 2001–2003. Being predominantly in more remote areas (≥5 kilometres away from a health centre or one hour walking time) with higher malaria prevalence, they covered 2030 villages by the end of 2013 (14% of the national total and 32% of the villages in the 34 ODs currently included in the VMW programme, Figure 11). In 2013 49% of total cases were detected by VMWs. Since their introduction, VMWs have detected 331,319 RDT positive cases of malaria. From 2004–2013, 23,055 (7.0%) of those detected were referred to government facilities due to having severe malaria or having another clinical episode of malaria within one month of initial treatment. Most of those referred would thus also have been captured in the HIS data. Thus at least 308,264 additional cases of malaria not included in the HIS data were detected by VMWs from 2004 to 2013. This gives a total of 802,540 cases nationwide from 2004 to 2013. With the same rate of referral there would have been 40,278 total cases with an API of 2.7 in 2013.
Until 2009, VMWs used only parasite histidine-rich protein 2 (p-HRP2) based P. falciparum RDTs (Paracheck Pf®) to test people for malaria thus only P. falciparum cases were detected (Figure 12). Since 2009, these have gradually been replaced by lactate dehydrogenase (LDH) plus HRP2-based RDTs (CareStart™), which can detect both P. falciparum and P. vivax.
With the scale-up of VMWs, the number of P. falciparum and P. vivax malaria cases detected by HIS in ODs with VMWs has markedly decreased since 2006 (Figure 13A) compared to those without VMWs (Figure 13B). However, the number of individuals recorded as tested for malaria in the HIS system in these ODs remained steady and the number of cases detected by VMWs fell from 2009. This suggests that, rather than being due to infected individuals attending VMWs instead of government clinics and hospitals, the fall in cases in the HIS system was due to a true decrease in malaria. Numbers of cases of P. vivax malaria increased in both from 2009–2011 and numbers of cases of both species have decreased similarly in all areas since 2012. The decrease in malaria mortality was more marked in ODs with VMWs since the large scale-up of coverage with VMWs from 2008 – 2009 (Figure 13C).
There have been VMWs in Pailin Province since 2009. They have gradually increased in number to cover all 114 villages with 228 VMWs and an additional 45 mobile malaria workers covering 30 of the 114 villages by the end of 2013. In addition, long-lasting insecticide-treated bed nets were distributed in 2009 in Pailin Province, with virtually 100% coverage of households by the end of that year. The number of P. falciparum cases detected by VWMs in Pailin Province greatly decreased from 2009 onwards, paralleling the decrease seen in the HIS data (Figure 14A). The number of people with P. vivax and number of people tested for malaria did not change from 2009 to 2013 (Figures 14B and C).
In the northeast of Cambodia, VMWs started work in 2004 following small-scale pilots and the number of cases of P. falciparum detected by them remained steady until 2009 (Figure 15A). Numbers of P. vivax detected by VMWs in the Northeast increased from mid-2009 (Figure 15B). The number of people tested each year did not change (Figure 15C). These patterns seen in the MIS paralleled those reported through HIS.
The overall number of cases of P. falciparum and P. vivax malaria attending public sector facilities in Cambodia has fallen markedly by 59% since 2011. This coincided with a rapid scale-up of distribution of insecticide treated bed nets and coverage of villages in high-risk areas by village malaria workers. P. falciparum remained relatively stable from 2004–2011 whilst numbers of P. vivax cases increased despite substantial distribution of ACT and the roll-out of RDTs during that period. The reasons for this delayed fall in cases are unclear but further examination is warranted to derive lessons for malaria control programmes elsewhere. The fall in percent of tests for malaria which were positive since 2011 with similar annual numbers tested throughout suggests the decline in incidence of clinical malaria was real and not an artefact due to changing surveillance efforts. Additionally, it appears that more effort has been put into testing to detect the same number of cases, particularly in the low season. This may be masking an earlier actual decline in P. falciparum in 2010 and 2011.
The marked increase in P. vivax in this study began a year too early for it to be related to the decrease in P. falciparum[11, 12]. Alternatively, it could be a result of improved diagnosis with introduction of a HRP-2/pLDH RDT (CareStart™) in 2009 in place of a Pf only HRP2 RDT (Paracheck Pf®), improved microscopic differentiation of species on diagnosis and better sensitivity of testing with development of the malaria control programme and improvements in training of microscopists from 2009 . Other differences between P. falciparum and P. vivax included different timing of seasonal peaks in cases and differences in the geographical distribution in numbers of cases over time. A greater relative decline in cases of P. falciparum than P. vivax and differences in the periodicity were also seen in Peru, particularly in mountain and jungle regions with some influence of precipitation and temperature . In Cambodia, further investigation of the influence of climatic factors and malaria control measures on the two species over space and time would be informative.
The decrease in mortality found in this study is likely to be due to improvement in healthcare provision during the study period. The difference in mortality in VMW and non-VMW areas in the HIS data suggest that VMWs may have contributed to this decreased mortality, presumably through earlier effective diagnosis, treatment and onward referral at a village level.
The roll-out of VMWs across remote and high transmission areas of Cambodia has resulted in detection of a large previously unrecorded burden of disease. With the scale-up of VMWs to over 40% of the villages in the higher transmission areas of Cambodia in the past five years, the total number of detected cases of both species nationally has almost doubled. Numbers of people reported through HIS did not fall during the roll-out of VWMs. This suggests that many of those people now presenting to VMWs previously sought care in the private sector and that the cohort who previously accessed public sector healthcare services for diagnosis and treatment of malaria continue to do so. If VMWs were rolled out further, additional increases in the detected caseload would thus be expected. VMWs have the advantage of being accessible to villagers who may not otherwise attend public sector facilities with their malaria. In the absence of VMWs, a high proportion of individuals are thought to purchase anti-malarials from the private sector. This was estimated at 87% in a survey in 2002  and 54% in 2011 . Despite the intensified malaria control efforts in and around Pailin, 43% were estimated to have received treatment from the private sector in 2011 . It is likely that many of these will choose instead the free testing and treatment available from the VMWs as had been shown previously  and in a recent evaluation by CNM . As data were not available on attendees at private pharmacies and clinics during the time of the study, the total burden of malaria in Cambodia remains unclear although it is evident from these data that it is grossly underestimated. This was suggested in a previous study, which showed that this underestimate is most prominent in remote areas of the country . A conservative estimate for total malaria burden in Cambodia from the present study would be over 100,000 cases per year from 2004–2011, falling to 70,000 in 2012 and 42,000 in 2013. These figures do not include those who sought treatment in the private sector (conservative estimates being over 50%) and it is not clear how many of these would attend a VMW if available. In 2013, VMWs were present in 44% of the villages in ODs with high malaria prevalence. If malaria prevalence across all villages in these ODs with VMWs were similar, then the actual number of cases could be 1/0.44 = 2.3 times the current number detected by VWMs i.e. 47,000. This would give a total of around 68,000 malaria cases per year in Cambodia in 2013, an API of 4.6 per 1000. Similarly for 2012, the estimated API using this method would have been 7.5 per 1000. This is 2.8 times higher than the estimate from WHO for that year of 2.7/1000 . In 2015, the private sector will no longer be permitted to diagnose or treat malaria. A more accurate picture of malaria burden in Cambodia can be expected as a result. By providing free testing and treatment, VMWs could be expected to increase the number of people being treated for malaria. This should lead to a decrease in transmission overall in areas with VMWs. This study confirmed this with a greater decrease in cases of P. falciparum in ODs with VMWs compared to ODs with no VMWs. Malaria mortality was also lower in ODs with VMWs highlighting the benefits of early effective diagnosis and treatment (EDAT).
During the study period, the geographical distribution of malaria in Cambodia has changed. This is likely to be due to a combination of factors including changes in malaria control activities, migration and deforestation. In some areas, particularly in parts of the west of Cambodia, P. falciparum decreased. Pailin Province had the greatest decrease in P. falciparum cases from 2007 onwards. It is not clear which factors caused this decrease although it coincided with the beginning of a period of intense focus on Pailin as the location of newly discovered artemisinin resistance . This resulted in intensification of malaria control activities in the area including increased availability of ACT and intensified active surveillance, such as focused and mass screening and treatment (FSAT and MSAT). It was also around this time that long-lasting insecticide treated bed nets were introduced . Additionally, extensive deforestation has occurred in Pailin during this period, resulting in very little forest cover in 2013. Before 2007, Pailin was the OD with the highest malaria transmission in Cambodia (API/1000 = 244 in 2004). That the surrounding Province of Battambang experienced a coincident decline in malaria, whilst other parts of Cambodia did not, may reflect the decreasing influence of the Pailin malaria ‘hot spot’ on adjacent areas. As of 2012, every village in Pailin has a VMW and this is likely to result in both improved collection of malaria data and a further decrease in the burden of disease through EDAT and directly-observed therapy (DOT). It is perhaps reassuring that the large decrease in malaria burden in Pailin was achieved despite the presence of reduced parasite clearance rates with artemisinins. However, reductions in disease burden were much less in Pursat Province where artemisinin resistance has also been identified  and bed net coverage has been high. It is possible that this difference between Pursat and Pailin is due to there having been far fewer VMWs and less deforestation in Pursat.
The northeast of Cambodia differed from the rest of the country with an increase in P. falciparum from 2007–2010 and, despite recent decreases in disease burden, is now the area with highest transmission intensity. There was also an even larger proportional increase in P. vivax than that seen nationally since 2009. Reasons for this are not clear. The Northeast is a relatively remote area with remaining high forest cover and some parts have had relatively lower levels of malaria control activity than elsewhere in Cambodia. Although VMWs have been present in the Northeast since 2003, there have been relatively few in 2 of the 4 provinces (Steung Treng and Kratie). Additionally few ITNs had been distributed in Steung Treng to the end of 2012, although the rest of the Northeast had a high proportion of households with ITN. The main focus for malaria control recently has been on Pailin and other areas with or suspected to have artemisinin resistance . Changing seasonal migration between the Northeast and the highly populated south of the country is one possible factor worth exploring further . The contribution of ongoing deforestation in parts of Cambodia to the reduction in malaria also needs to be investigated further.
In 2013, the Provinces bordering Laos, Vietnam and Thailand in the north and northeast of the country had the highest transmission intensity for both species. These borders are frequented by migrant and mobile populations who have proven difficult to target for surveillance and malaria control activities. A recent study by CNM found 14% of individuals crossing the Cambodia-Laos border at one location in Steung Treng to be positive for malaria by polymerase chain reaction (PCR) and two thirds of these were asymptomatic (unpublished data). This raises the possibility that importation of malaria from neighbouring countries is contributing to the higher incidence of infection found in the border areas of Cambodia. Migrant populations more often delay seeking appropriate diagnosis and treatment and are a potential source and means of dispersal of anti-malarial drug resistance, which is frequently first identified in border areas [25, 26].
This study had several limitations. This study did not measure transmission intensity directly. Instead, API was used as a surrogate measure. Although the majority of cases are likely to have been infected in the same district as where they were diagnosed, it should be noted that for some individuals transmission may have occurred elsewhere. Malaria surveillance data are notoriously prone to collection bias. Self-treated and privately treated cases including those treated by private pharmacies and traditional healers were not captured by these data and migrant and mobile populations were probably underrepresented. Although it is likely that many of these underrepresented cases chose to attend VMWs where available, the true numbers of such cases are not known. Remote populations are also thought to have been underrepresented by the HIS data ; however, most of these communities are now included in the VMW project. Changes in efficiency of the systems used to collect the data are difficult to quantify and may introduce biases. The strength of these data is that several trends are apparent over time in the two independent datasets. During the study period, there was widespread deforestation, and the contribution of this to the reduction in malaria, transmitted predominantly by forest-dwelling mosquitoes was not examined in this study.
This analysis highlights a number of areas requiring further detailed study. Further investigation of trends over time in individual ODs matched with details of malaria control interventions employed, changes in environmental determinants and investigation of the contribution of mobile and migrant populations would be highly informative for planning future malaria control activities. In particular detailed data on ACT use have been collected in large national surveys and collection of information on the behavior and malaria burden in mobile and migrant populations (MMPs) is ongoing and a National MMP Strategy has been developed . The VMW project collects more detailed data than we have had space to present here in this paper including age, gender, migration status, pregnancy status and breakdown by individual village. This growing dataset is an invaluable resource for the future for Cambodia. By combining these data with other studies currently being undertaken, for example, on population migration and the geographical distribution of artemisinin resistance, important new insights will be gained.
This study has identified several major trends in malaria caseload in Cambodia over the past decade which warrant further investigation. This includes a marked decrease in P. falciparum malaria since 2009 coincident with scale-up of insecticide treated bed nets and village malaria workers. Although malaria surveillance data are notoriously prone to collection bias, the use of data from two independent sources greatly increases the robustness of the findings.
Artemisinin combination therapy
Annual parasite incidence
National Centre for Parasitology, Entomology and Malaria Control
Directly observed therapy
Early effective diagnosis and treatment
Health Information System
Insecticide-treated bed net
Malaria Information System
Mobile and migrant populations
Mobile malaria worker
Polymerase chain reaction
Parasite histidine-rich protein 2
Rapid diagnostic test
Village malaria worker.
World Health Organization: Global Partnership to Roll Back Malaria: Global plan for artemisinin resistance containment (GPARC). 2011, Geneva: World Health Organization,
White NJ: Artemisinin resistance–the clock is ticking. Lancet. 2010, 376: 2051-2052. 10.1016/S0140-6736(10)61963-0.
Dondorp AM, Fairhurst RM, Slutsker L, Macarthur JR, Breman JG, Guerin PJ, Wellems TE, Ringwald P, Newman RD, Plowe CV: The threat of artemisinin-resistant malaria. N Engl J Med. 2011, 365: 1073-1075. 10.1056/NEJMp1108322.
Phyo AP, Nkhoma S, Stepniewska K, Ashley EA, Nair S, McGready R, ler Moo C, Al-Saai S, Dondorp AM, Lwin KM, Singhasivanon P, Day NP, White NJ, Anderson TJ, Nosten F: Emergence of artemisinin-resistant malaria on the western border of Thailand: a longitudinal study. Lancet. 2012, 379: 1960-1966. 10.1016/S0140-6736(12)60484-X.
Kyaw MP, Nyunt MH, Chit K, Aye MM, Aye KH, Aye MM, Lindegardh N, Tarning J, Imwong M, Jacob CG, Rasmussen C, Perin J, Ringwald P, Nyunt MM: Reduced susceptibility of Plasmodium falciparum to artesunate in southern Myanmar. PLoS One. 2013, 8: e57689-10.1371/journal.pone.0057689.
Tran TH, Nguyen TT, Nguyen HP, Boni MF, Ngo VT, Nguyen TN, Le HT, Cao QT, Pham VT, Phung DT, Le TL, Le TD, Merson L, Dolecek C, Stepniewska K, Ringwald P, White NJ, Farrar J, Wolbers M: In vivo susceptibility of Plasmodium falciparum to artesunate in Binh Phuoc Province. Vietnam Malar J. 2012, 11: 355-
Maude RJ, Socheat D, Nguon C, Saroth P, Dara P, Li G, Song J, Yeung S, Dondorp AM, Day NP, White NJ, White LJ: Optimising strategies for Plasmodium falciparum malaria elimination in cambodia: primaquine, mass drug administration and artemisinin resistance. PLoS One. 2012, 7: e37166-10.1371/journal.pone.0037166.
Maude RJ, Pontavornpinyo W, Saralamba S, Aguas R, Yeung S, Dondorp AM, Day NP, White NJ, White LJ: The last man standing is the most resistant: eliminating artemisinin-resistant malaria in Cambodia. Malar J. 2009, 8: 31-10.1186/1475-2875-8-31.
Average Monthly Rainfall and Temperature for Cambodia from 1990–2009: Average Monthly Rainfall and Temperature for Cambodia from 1990–2009. [http://sdwebx.worldbank.org/climateportal/index.cfm?page=country_historical_climate&ThisRegion=Asia&ThisCCode=KHM]
National Institute of Statistics - Ministry of Planning, Cambodia: General Population Census of Cambodia 2008. [http://nada.nis.gov.kh/index.php/catalog/1]
Chaves LF, Kaneko A, Pascual M: Random, top-down, or bottom-up coexistence of parasites: malaria population dynamics in multi-parasitic settings. Ecology. 2009, 90: 2414-2425. 10.1890/08-1022.1.
Haghdoost AA, Alexander N: Systematic review and meta-analysis of the interaction between Plasmodium falciparum and Plasmodium vivax in humans. J Vector Borne Dis. 2007, 44: 33-43.
Ministry of Health National Centre for Parasitology EaMC: Annual progress report of the National for Parasitology Entomology and Malaria Control Program 2009. 2009, Phnom Penh: Ministry of Health, Cambodia
Chowell G, Munayco CV, Escalante AA, McKenzie FE: The spatial and temporal patterns of falciparum and vivax malaria in Perú: 1994–2006. Malar J. 2009, 8: 142-10.1186/1475-2875-8-142.
Yeung S, Van Damme W, Socheat D, White NJ, Mills A: Access to artemisinin combination therapy for malaria in remote areas of Cambodia. Malar J. 2008, 7: 96-10.1186/1475-2875-7-96.
ACT Watch Group and PSI/Cambodia: Kingdom of Cambodia household survey report, 2011. 2011, Phnom Penh, Cambodia: Phnom Penh, Cambodia
CanavatidelaTorre S, Ly P, Nguon C, Roca-Feltrer A, Sintasath D, Whittaker M, Singhasivanon P: Evaluation of community malaria worker performance in western Cambodia: a quantitative and qualitative assessment. Am J Trop Med Hyg. 2012, 87: 106-
Incardona S, Vong S, Chiv L, Lim P, Nhem S, Sem R, Khim N, Doung S, Mercereau-Puijalon O, Fandeur T: Large-scale malaria survey in Cambodia: novel insights on species distribution and risk factors. Malar J. 2007, 6: 37-10.1186/1475-2875-6-37.
WHO: Malaria Country Profiles 2013. 2013, Cambodia. Geneva: Worl;d Health Organization
Maude RJ, Woodrow CJ, White LJ: Artemisinin antimalarials: preserving the "Magic Bullet". Drug Dev Res. 2010, 71: 12-19.
Ministry of Health National Centre for Parasitology EaMC: Annual progress report of the National for Parasitology Entomology and Malaria Control Program 2007. 2007, Phnom Penh: Ministry of Health National Centre for Parasitology, Entomology and Malaria Control
Amaratunga C, Sreng S, Suon S, Phelps ES, Stepniewska K, Lim P, Zhou C, Mao S, Anderson JM, Lindegardh N, Jiang H, Song J, Su XZ, White NJ, Dondorp AM, Anderson TJ, Fay MP, Mu J, Duong S, Fairhurst RM: Artemisinin-resistant Plasmodium falciparum in Pursat province, western Cambodia: a parasite clearance rate study. Lancet Infect Dis. 2012, 12: 851-858. 10.1016/S1473-3099(12)70181-0.
Ministry of Health National Centre for Parasitology EaMC: Annual progress report of the National for Parasitology Entomology and Malaria Control Program 2010. 2010, Phnom Penh: Ministry of Health National Centre for Parasitology, Entomology and Malaria Control
Socheat D: Migrants and mobile populations: progress made. 2nd International Task Force Meeting for the Strategy for the Containment of Artemisinin Resistant Malaria Parasites in South-East Asia Project. 2010, Hanoi, Vietnam: National Centre for Parasitology, Entomology and Malaria Control (CNM), Cambodia
Bhumiratana A, Intarapuk A, Sorosjinda-Nunthawarasilp P, Maneekan P, Koyadun S: Border malaria associated with multidrug resistance on Thailand-Myanmar and Thailand-Cambodia borders: transmission dynamic, vulnerability, and surveillance. Biomed Res Int. 2013, 2013: 363417-
Lynch C, Roper C: The transit phase of migration: circulation of malaria and its multidrug-resistant forms in Africa. PLoS Med. 2011, 8: e1001040-10.1371/journal.pmed.1001040.
Canavati S, Nguon C, Guyant P, Roca-Feltrer A, Yeung S: Strategy to address migrant and mobile populations for malaria elimination in Cambodia MMP strategy-March 2013. 2013, Phnom Penh: National Center for Parasitology EaMCC ed
The authors would like to thank the staff at CNM and healthcare workers throughout Cambodia who collected and assimilated the data used in this study. Mahidol-Oxford Tropical Medicine Research is funded by the Wellcome Trust of Great Britain.
The authors declare that they have no competing interest.
RJM designed the study, performed the analyses and wrote the report. CN, PL, TB and PN arranged collection of the data. PL, PN, SEC, AMD and LJW wrote the report. All authors approved the final version of the manuscript.
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