Survival of immature Anopheles arabiensis (Diptera: Culicidae) in aquatic habitats in Mwea rice irrigation scheme, central Kenya
© Mwangangi et al; licensee BioMed Central Ltd. 2006
Received: 30 October 2006
Accepted: 24 November 2006
Published: 24 November 2006
The survivorship and distribution of Anopheles arabiensis larvae and pupae was examined in a rice agro-ecosystem in Mwea Irrigation Scheme, central Kenya, from August 2005 to April 2006, prior to implementation of larval control programme.
Horizontal life tables were constructed for immatures in semi-field condition. The time spent in the various immature stages was determined and survival established. Vertical life tables were obtained from five paddies sampled by standard dipping technique.
Pre-adult developmental time for An. arabiensis in the trays in the experimental set up in the screen house was 11.85 days from eclosion to emergence. The mean duration of each instar stage was estimated to be 1.40 days for first instars, 2.90 days for second instars, 1.85 days for third instars, 3.80 days for fourth instars and 1.90 days for pupae. A total of 590 individuals emerged into adults, giving an overall survivorship from L1 to adult emergence of 69.4%. A total of 4,956 An. arabiensis immatures were collected in 1,400 dips throughout the sampling period. Of these, 55.9% were collected during the tillering stage, 42.5% during the transplanting period and 1.6% during the land preparation stage. There was a significant difference in the An. arabiensis larval densities among the five stages. Also there was significant variation in immature stage composition for each day's collection in each paddy. These results indicate that the survival of the immatures was higher in some paddies than others. The mortality rate during the transplanting was 99.9% and at tillering was 96.6%, while the overall mortality was 98.3%.
The survival of An. arabiensis immatures was better during the tillering stage of rice growth. Further the survival of immatures in rice fields is influenced by the rice agronomic activities including addition of nitrogenous fertilizers and pesticides. For effective integrated vector management, the application of larvicides should target An. arabiensis larvae at the tillering stage (early vegetative stage of rice) when their survival in the aquatic habitats is high to significantly reduce them and the larvicides should be long-lasting to have a significant impact on the malaria vector productivity on the habitats.
Vector-borne diseases are among the most important public health problems and obstacles to socioeconomic development of developing countries, particularly in the tropics, with malaria alone causing an estimated 1.5 – 2.7 million deaths and 300 – 500 million cases per year. Flood irrigation during rice cultivation has long been associated with an increase in number of disease vectors and corresponding increased health burden due to malaria and other vector and water-borne diseases. In the Mwea irrigation scheme, central Kenya, rice growing requires irrigation almost throughout the crop cycle. The Anopheles gambiae complex and the Anopheles funestus complex are the primary vectors of malaria in Mwea rice irrigation scheme. The distribution and abundance of mosquito larvae results from availability of oviposition sites, the oviposition preferences of females and the ability of the immatures to tolerate and develop after the eggs were laid.
Life tables provide a structured framework for identifying developmental stages most susceptible to mortality and, under some conditions, for inferring sources of larval deaths. The life tables for the developing immatures can be constructed using either horizontal or vertical methods [1–4]. Horizontal life table methods are appropriate for distinct cohorts that can be followed through time, whereas vertical life table methods are appropriate for populations with overlapping generations and age distributions that remain stationary for the duration of the sampling period. Since vector abundance is a critical factor in the mosquito-malaria cycle, generational life-tables is a useful tool in malaria early warning systems for the purpose of forecast, early detection of epidemics and intensity of disease prevalence in holoendemic areas. Service [5–7], Reisen and Siddiqui  and Reisen et al. provide extensive discussions about how such information can be analysed.
In Kenya, Service [5–7] and Aniedu  studied the survival of immature An. gambiae complex in the larval habitats. The objective of this study was to determine survival of immature Anopheles arabiensis in different habitats in the Mwea irrigation scheme and under the experimental rice growing conditions prior to implementation of a larval control programme.
The studies were conducted 100 km North East of Nairobi, in Mwea Irrigation and Agricultural Development Center (MIAD) within the Mwea irrigation scheme. Mwea occupies the lower altitude zone of Kirinyaga District in an expansive low-lying area. The mean annual rainfall is 950 mm with maximum amount falling in April/May (long rains) and October/November (Short rains). The average maximum temperatures are in the range of 16–26.5°C. Relative humidity varies from 52–67%. According to the 1999 national census, the Mwea irrigation scheme has approximately 150,000 persons in 25,000 households. The Mwea rice irrigation scheme is located in the west central region of Mwea Division and covers an area of about 13,640 ha. More than 50% of the scheme area is used for rice cultivation. The remaining area is used for subsistence farming, grazing and community activities.
Adult mosquito sampling was done at the Kariwa village, which is nearby the MIAD center. Kariwa has approximately 68 homesteads with over 350 residents. Cows, goats, chickens, and donkeys are the primary domestic animals. These animals are kept within 5 m of most houses. Over 95% of the houses have mud walls with iron roofing.
Horizontal life tables semi field condition
Blood-fed An. arabiensis females were collected with aspirators from houses  throughout Kariwa village and kept in cages containing 2% sugar solution in a semi field condition at the Mwea Irrigation and Agricultural Development Center (MIAD) field station until they became gravid. A screen house constructed at the MIAD centre provided the semi field condition. Petri dishes containing a small amount of distilled water were placed inside the cages to attract oviposition. Newly laid eggs in these containers were monitored every 0.5 h to determine their time of eclosion. Approximately 2 h after eclosion, 50 first-instar larvae (L1) were collected for each replicate. These were transferred to larval rearing pans (30 by 24 cm) at an initial density of 14 larvae/cm2 of pan surface area (L/cm2). The screen house provided a semi field condition to the mosquito larvae during the entire development period. Briefly the screen house was constructed with wooden framework and plastic mosquito netting pinned at the sides. The plastic netting material was reinforced with chicken wire mesh. The roof was made of translucent roofing material ensuring the penetration of sunlight. The mosquito proof netting ensured that any ovipositing adults were excluded from the trays inside the screen house. The mosquito larvae rearing were done using water collected from the inlet canal to the paddies in the field station. The nutrient for larvae development was found in the floating particles in this water. The water was changed in the trays every other day. Room temperature and relative humidity were monitored daily every three hours using a HOBO® (Onset Computer Corporation, Bourne, Massachusetts, USA). BoxCar Pro (Onset Computer Corporation, Bourne, Massachusetts, USA) was used to download the weather information at the end of the study duration.
Duration of the preadult development period were determined by observing each pan at 09.00 and 16.00 h daily, at each time all larval skins were removed, scored to instar, and counted. The larval cuticle of L1 was identified by distinctive dark head capsules with semi-transparent abdominal exuviae at around 24 to 40 h after the pans were established. The number of larvae metamorphosing to the next instar could thus be inferred. The time taken for 50% of the larvae in a pan to change to the next instar was taken as the median developmental period of that specific stage. Computations of immature stage duration and survivorship followed those of Reisen et al.. A sub sample of emergent An. gambiae s.l. adults were further identified using rDNA Polymerase Chain Reaction (PCR) technique into the sibling species .
When all adults had emerged, stage-specific survivorship for the pooled data was estimated according to method used by Edillo et al :
S i = n i /( n i - 1),
n i = total number of immatures entering life instar i,
and n i - 1 = the number alive in the previous instar.
Mean instar duration in hours at molting, T i, was:
D i = T i - ( t i-1 ),
where ti-1 was the previous mean age at molting.
The percentage of total immature life spent at each instar was:
L i = 100 × D i/t 5 ,
where t5 was the median time of adult emergence.
Relativized probability of capture in a vertical sample was P i = D i /D p ,
where Dp was the duration of the shortest-lived life stage, which was taken as the standard.
Survivorship from L1 to adult emergence was estimated by A/I,
where A = total number of adults
and I = total number of L1 originally counted into the rearing trays.
Vertical life table
Five paddy habitats were randomly selected in MIAD centre rice fields for the generation of vertical life tables. Larval sampling was done using standard dipping technique. The larvae collected per dip were counted and scored to life stage . The contents of each dipper were poured into a white tray and the mosquito larvae counted and age-graded. The larvae were grouped according to species and age graded as early instars (L1, L2), late instars (L3, L4) and Pupae (P). The larval sampling was done in from land preparation to rice tillering stage. This period of rice growing has been associated with increase in Anopheles larval densities and diversity . In each season the larval habitats selected was sampled four times (once every week). A sub-sample of An. gambiae s.l. late stage instars larvae were further identified using rDNA Polymerase Chain Reaction (PCR) technique into the sibling species .
Numbers of immatures in each habitat
The distribution of immatures per habitat was compared in a contingency table. The immature age An. arabiensis was categorized as early instars (L1 + L2), late stage instars (L3 + L4) and pupae (P), and the chronological collection periods were based on rice growth cycle grouped as land preparation, transplanting and tillering stage. These were then analysed statistically as a 3 (age groups) × n (habitats) × n (sampling periods) contingency table.
Measurement of physicochemical variables
Temperature, pH, conductivity, dissolved oxygen, salinity, Total Dissolved Solids (TDS), nitrates, phosphates, ammonia and sulphate content were measured in the paddies. Briefly, pH, conductivity, dissolved oxygen and temperature was measured using hand held machine YSI 650 Multiparameter Display System (YSI Environmental, YSI Incorporated, Yellow Springs, USA). Salinity and Total Dissolved solids (TDS) were measured using field hand held equipment YSI EC 300 (YSI Environmental, YSI Incorporated, Yellow Springs, USA). Nitrates, phosphates, ammonia and sulphate content were measured using spectrophotometric technique by use of portable HACH machines (HACH®, DR/2400 Spectrophotometer, Hach Company, Ames, Iowa). All physicochemical parameters were measured on site at the time of mosquito larval sampling.
Age distribution and survivorship curves
Instar mortalities of Anopheles arabiensis at the screen house
Age at the beginning of Instar
No. entering instar
Deaths in instar
Relative proportion dying in Instar
Proportion dying daily in instars
Vertical life tables
The total number of An. arabiensis immatures collected from paddies at each rice growth cycle
Early instars Anopheles
Late instars Anopheles
The Mean, variance (s2) and coefficient of dispersion (CD) of the densities of An. arabiensis larvae collected from each paddy.
Physicochemical conditions of the paddies
Mean and Standard Error (SE) of physicochemical conditions of water samples taken from paddies with different rice stage
22.8 ± 0.87
24.25 ± 1.07
24.22 ± 0.70
301.5 ± 45.11
317.20 ± 60.30
178.72 ± 15.45
319.54 ± 23.01
240.44 ± 45.07
159.52 ± 18.32
0.2 ± 0.01
64.92 ± 14.04
91.89 ± 13.71
7.86 ± 0.15
7.85 ± 0.10
7.61 ± 0.06
13.6 ± 3.44
25.9 ± 4.99
12.64 ± 5.09
80 ± 5.25
54.4 ± 11.00
68.22 ± 28.59
Nitrogen Ammonia (mg/l)
0.01 ± 0.001
4.1 ± 0.080
0.14 ± 0.02
Distribution of immatures Anopheles arabiensis in the paddy
This study found that survivorship of An. arabiensis larvae in paddies was higher at the tillering stage than at the transplanting stage. At the tillering stage there is addition of inorganic nitrogenous fertilizers. The addition of the nitrogenous fertilizers acts as the attractant for oviposition by the gravid An. arabiensis mosquitoes. Broadcasting nitrogenous fertilizers in rice fields has been found to enhance mosquito larval populations [15, 16]. Also few days after transplanting, there is addition of nitrogenous fertilizers, which has been shown to attract Anopheles and culicine mosquito oviposition [16, 17]. Furthermore, there was a strong positive correlation in between fertilizer application and physicochemical variables tested. There was an increase of nitrates, sulphate, nitrogen ammonia and dissolved oxygen immediately after transplanting which is attributed to addition of nitrogen fertilizers. It was hypothesized that the changes in these physicochemical variables acted first as oviposition attractant resulting in higher densities of Anopheles larvae and secondly high nutritional resources, which ensured high survivorship of these larvae in the paddies. The nitrogenous fertilizer application enhanced the food resource available for the anopheline immatures stages that increases their survival and eventually resulting to higher vector productivity.
The study has demonstrated that survivorship of An. arabiensis mosquito larvae from first instar to adult emergence was lower in the paddies. This is an indication that a higher proportion of larvae and pupae died in the paddy habitats. The findings in this study were similar to that of Sunahara et al.  who found out that the causes of mortality in the rice fields were caused by both abiotic and biotic factors. Service  attributed the efficacy of the natural enemies in such small habitats that may be inhabited by mosquito larvae to the small volume of water in them. When eggs are laid in a given habitat, mosquito competitors and predators can reduce mosquito densities by direct mortality and/or reduced growth rates due to reduced activity and due to reduced food availability.
In the experimental set up, there was significant reduction in the immatures. In the trays all larval instars were found together. Koenraadt and Takken  showed that within the habitats, all four larval instars were found together. In this set up majority of dead larvae were not recovered from the trays with L4 present suggesting that there was cannibalism occurring at the trays. Cannibalism and predation are important factors in reducing the larval survivorship in the habitats especially the small sized habitats. Koenraadt and Takken  and Koenraadt  showed that the older larval instars (L4) in the trays reduced the numbers of earlier instars stages.
This study also demonstrated that high proportion of larvae died in the 1st and 3rd instars as well as in pupal stage. The overall mortality was significantly high in all the habitats, being 98.26%. These high mortality rates are consistent with survivorship rates results of An. arabiensis and with larval mortality rates results by other workers under field conditions. Service  recorded 92.6–100% mortality for An. gambiae rice fields and temporary pools, Kano plains Kisumu, while Aniedu et al.  recorded a 91.9% for An. gambiae in Baringo, Kenya. The high mortality in the present study can be attributed partly to predation since presence of known and potential An. arabiensis larval and pupal predators were recorded although quantification of the relative contribution or proportion of mortality of the mosquito immature stages that was due to predation was not conducted. Some of the families of the other non-mosquitoes invertebrates observed in this study have been noted as potential predators in some previous studies in western Kenya [6, 7]. However, previous estimates indicate that significant mosquito larval and pupal population reduction, 50–90% in Culex tritaeniorhynchus  and 82% in An. gambiae mosquitoes  was due to predation effects.
It was further observed that when the paddies were sprayed with Fenitrithion was applied at a rate of 400 ml/acre, 35 days after transplanting to control insect pests, all the An. gambiae larvae died and the survival was zero. The Fenitrithion also killed all the other invertebrates within the paddy indiscriminately. The zero percent mortality continued for about ten days when the colonization started again in the paddies. The application of the pesticide was done together with topdressing with sulfate of ammonia. The nitrogenous fertilizer attracted the gravid Anopheles mosquitoes were at the same time attracted to oviposit consequently with the diminishing effect of the pesticide the An. arabiensis larval densities and survival increased significantly. This increased the survivorship of immatures significantly as the other non-mosquito invertebrates were very low in densities. More detailed ecological studies are required to elucidate the importance of predators and pathogens in regulating population size of the immature stages of An. arabiensis in the rice fields of Mwea Irrigation Scheme during the rice growing cycle and to identify other limiting factors governing their numbers and local distribution. In conclusion, the survival of An. arabiensis immatures was better during the tillering stage of rice growth. Further the survival of immatures in rice fields is influenced by the rice agronomic activities including addition of nitrogenous fertilizers and pesticides. For effective integrated vector management, the application of larvicides should target An. arabiensis larvae based on the early developmental stages of rice. The larvae should be targeted at the tillering stage (early vegetative stage) when their survival in the aquatic habitats is high to significantly reduce them and the larvicides should be long lasting to have a significant impact on the malaria vector productivity on the habitats.
We are grateful to Prof. Christian Baumgartner, Director General International Centre of Insect Physiology and Ecology for his strong support of this project. We acknowledge the data collection provided by Enock Mpanga, James Wauna, Peter Wekoyela, Peter Mutiga, William Waweru, Nelson Maingi, Martin Njigoya, Paul Mwangi, Christine Maina, Isabel Marui, Susan Mugo, Nicholus Gachoki, Charles Kiura, Gladys Karimi, Irene Kamau, Julius Murimi, Naftaly Manegene. This paper is published with the permission of the Directors of International Centre of Insect Physiology and Ecology and Kenya Medical Research Institute. The study was funded by NIH/NIAID grant U01-A154889 (Robert Novak).
- Service M: Mosquito Ecology: Field Sampling Methods. Elsevier Applied Science. 1993, SecondGoogle Scholar
- Reisen WK, Siddiqui TF: Horizontal and vertical estimates of immature survivorship for Culex tritaeniorhynchus (Diptera: Culicidae) in Pakistan. J Med Entomol. 1979, 16: 207-218.View ArticlePubMedGoogle Scholar
- Reisen WK, Mahmood F: Horizontal life table characteristics of the malaria vectors Anopheles culicifacies and Anopheles stephensi (Diptera: Culicidae). J Med Entomol. 1980, 17: 211-217.View ArticleGoogle Scholar
- Reisen WK, Azra K, Mahmood F: Anopheles culicifacies (Diptera: Culicidae): horizontal and vertical estimates of immature development and survivorship in rural Punjab Province, Pakistan. J Med Entomol. 1982, 19: 413-422.View ArticlePubMedGoogle Scholar
- Service M: Studies on sampling larval populations of the Anopheles gambiae complex. Bull World Hlth Organ. 1971, 45: 169-180.Google Scholar
- Service M: Mortalities of the larvae of Anopheles gambiae Giles complex and detection of predators by precipitin test. Bull Entom Res. 1973, 62: 359-369.View ArticleGoogle Scholar
- Service MW: Mortalities of the immature stages of species B of the Anopheles gambiae complex in Kenya: comparison between rice fields and temporary pools, identification of predators, and effects of insecticidal spraying. J Med Entomol. 1977, 13: 535-545.View ArticlePubMedGoogle Scholar
- Aniedu I, Mutinga MJ, Mutero CM: Vertical estimates of survivorship of larvae and pupae of Anopheles gambiae Giles Complex in Baringo District, Kenya. Insect Sci Applic. 1993, 14: 39-48.Google Scholar
- WHO: Manual on practical entomology in Malaria. Part II. Methods and techniques. World Health Organization Offset Publication, Geneva. 1975, 13:Google Scholar
- Scott JA, Brodgon WG, Collins FH: Identification of single specimens of Anopheles gambiae complex by Polymerase chain reaction. Am J Trop Med Hyg. 1993, 49: 520-529.PubMedGoogle Scholar
- Edillo FE, Toure YT, Lanzaro GC, Dolo G, Taylor CE: Survivorship and distribution of immature Anopheles gambiae s.l. (Diptera: Culicidae) in Banambani village. Mali. J Med Entomol. 2004, 41: 333-339.View ArticlePubMedGoogle Scholar
- Gillies MT, Coetzee M: A supplement to anophelinae of Africa south of Sahara(Afro-tropical region). Publication of the South Africa Institute of Medical Research. 1987, 55: 1-143.Google Scholar
- Mwangangi J, Shililu J, Muturi E, Gu W, Mbogo C, Kabiru E, Jacob B, Githure J, Novak RJ: Dynamics of immature stages of Anopheles arabiensis and other mosquito species (Diptera: Culicidae) in relation to rice cropping in a rice agro-ecosystem in Kenya. J Vector Ecol. 2006, 31:Google Scholar
- Southwood TR:Ecological methods. Methuen, London. 1966,Google Scholar
- Simpson I, Roger PA: Impact of agrochemicals on mosquito larvae populations in rice fields. International Rice Research Newsletter. 1991, 16: 29-30.Google Scholar
- Victor TJ, Reuben R: Effects of organic and inorganic fertilisers on mosquito populations in rice fields of southern India. Med Vet Entomol. 2000, 14: 361-368. 10.1046/j.1365-2915.2000.00255.x.View ArticlePubMedGoogle Scholar
- Mutero CM, Ng'ang'a PN, Wekoyela P, Githure J, Konradsen F: Ammonium sulphate fertiliser increases larval populations of Anopheles arabiensis and culicine mosquitoes in rice fields. Acta Trop. 2004, 89: 187-192. 10.1016/j.actatropica.2003.08.006.View ArticlePubMedGoogle Scholar
- Sunahara T, Ishizaka K, Mogi M: Habitat size: a factor determining the opportunity for encounters between mosquito larvae and aquatic predators. J Vector Ecol. 2002, 27: 8-20.PubMedGoogle Scholar
- Koenraadt CJM, Takken W: Cannibalism and predation among larvae among larvae of the Anopheles gambiae complex. Med Vet Entomol. 2003, 17: 61-66. 10.1046/j.1365-2915.2003.00409.x.View ArticlePubMedGoogle Scholar
- Koenraadt CJM, Majambere S, Hemerik L, Takken W: The effects of food and space on the occurrence of cannibalism and predation among larvae of Anopheles gambiae s.l. Entomologia Experimentalis et Applicata. 2004, 112: 125-134. 10.1111/j.0013-8703.2004.00186.x.View ArticleGoogle Scholar
- Mbogo CM, Mwangangi JM, Nzovu J, Gu W, Yan G, Gunter J, Swalm C, Keating J, Regens JL, Shililu JI, Githure JI, Beier JC: Spatial and temporal heterogeneity of Anopheles mosquitoes and Plasmodium falciparum transmission along the Kenyan coast. Am J Trop Med Hyg. 2003, 68: 734-742.PubMedGoogle Scholar
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