- Open Access
Malaria and obesity: obese mice are resistant to cerebral malaria
© Robert et al; licensee BioMed Central Ltd. 2008
- Received: 24 January 2008
- Accepted: 19 May 2008
- Published: 19 May 2008
The relationship between malaria and obesity are largely unknown. This is partly due to the fact that malaria occurs mainly in tropical areas where, until recently, obesity was not prevalent. It now appears, however, that obesity is emerging as a problem in developing countries. To investigate the possible role of obesity on the host-parasite response to malarial infection, this study applied a murine model, which uses the existence of genetically well characterized obese mice.
The receptivity of obese homozygous ob/ob mice was compared to the receptivity of control heterozygous ob/+ lean mice after a single injection of Plasmodium berghei ANKA sporozoites. Both parasitaemia and mortality in response to infection were recorded.
The control mice developed the expected rapid neurological syndromes associated with the ANKA strain, leading to death after six days, in absence of high parasitaemia. The obese mice, on the other hand, did not develop cerebral malaria and responded with increasing parasitaemia, which produced severe anemia leading to death 18–25 days after injection.
The observed major differences in outward symptoms for malarial infection in obese versus control mice indicate a link between obesity and resistance to the infection which could be addressed by malariologists studying human malaria.
- Post Infection
- Cerebral Malaria
- Obese Mouse
- Malarial Infection
Obesity, mainly characterized by an excess of adipose tissue, is associated with high risks of developing several pathologies that have disastrous consequences. This health condition is of paramount importance for public health in developed countries. For example, in the USA more than half of the population is considered to be obese according to the WHO criteria. Obesity has also been identified as an emerging condition in developing countries, especially in towns of tropical areas [1, 2].
Crude NCBI-PubMed searches with "obesity" and "malaria" yielded 107,545 and 46,653 references, respectively. However, association of the two terms produced only 17 entries, two of which partially addressed the issue [3, 4], indicating that the two communities of researchers occupy distinct scientific niches that do not overlap. A limited survey amongst fellow malariologists also revealed the lack of bridges between the two disciplines. Finally, a search in the French database of all recorded malaria cases (about 45,000) was eloquent: the bodyweight of the studied patients was simply not recorded.
Several studies have been performed on malaria and malnutrition in humans [5–7]. They strongly suggest that malaria is the major contributor to growth retardation in children living in endemic areas. Evidence from observational cohorts indicated that malnutrition decreases the susceptibility to malaria, and that this may be due to an interaction between the parasite and the host immune system . At the same time, it can also worsen the prognosis of malaria . However, the point at which malnutrition ceases to be protective and becomes an adverse prognosticator is not clear . Experiments with mice demonstrate that nutritional status interferes with the expression of malarial disease. Lean mice fed on a normal diet (25% protein) suffered severe parasitaemia and died within two weeks when infected with a lethal strain of Plasmodium yoelii, whereas mice fed on low-protein diet survived without apparent parasitaemia. This response has been related to an enhanced innate immunity in mice fed on low-protein diet . In another study, P. berghei malaria sporozoites were less infective in knock out LDLR-/- mice (low density lipoprotein receptors) maintained on a high fat diet, as compared to littermates maintained on a normal diet . While some studies suggest a link between nutritional status (malnutrition) and the evolution of malaria infections, little is known about the relationship of obesity vis-à-vis the prevalence or evolution of malarial infections in human . Considering the increasing world concern about obesity, on the one hand, and malaria on the other, any relationship between both clinical statuses would be of great significance.
This study investigated the possibility of such a relationship, using the murine model ob/ob, which provides valuable tools for studying obesity. The obese ob/ob mice (C57BL/6 background) have developed a spontaneous recessive and nonsense punctual mutation in the leptin gene which, in the mutant ob/ob homozygote, encodes for a truncated non-functional protein, resulting in the absence of retro-control on hunger. These leptin-deficient mice exhibit irrepressive feeding behaviour and develop a patent obesity . Leptin, a hormone mainly produced by adipocytes, is a central mediator of the neuroendocrine pathways involved in the control of food intake, basal metabolism and reproductive function. As a consequence, ob/ob mice exhibit a hyperlipidemia caused by an increase in hepatic triglyceride synthesis and secretion . The obese mice also develop hyperglycaemia and type 2 diabetes, which occurs as a consequence of obesity rather than a primary effect of leptin. Recent evidence also indicates that leptin acts as a proinflammatory cytokine .
A literature search indicates that obese mice have not been experimentally infected by malaria parasites previously. Obesity could play a protective, neutral or permissive role in the evolution of malarial infections and subsequent patho-physiological manifestations. However, many factors can interfere. Here, using the ANKA strain of P. berghei which induces cerebral malaria in C57BL/6 mice, it has been shown that the outcome of malaria infection differs dramatically between obese and control lean mice. Contrary to the control mice, the obese mice did not develop cerebral malaria.
Parasitaemias of Plasmodium berghei ANKA-infected C57BL/6 mice, as % of parasitized red blood cell.
Number of sporozoites per mouse
Day of infection
Number of mice
Number of mice
Number of mice
In contrast, the obese mice did not present any clinical sign of cerebral malaria (behavioral changes or coma). They died from severe anaemia between days 18 and 25 PI (those surviving at day 25 were in the final phase and sacrificed). Cox proportional hazard indicated a P-value of 2.8 × 10-5 and a death risk factor, at any moment, 7.34 times lower for obese mice. The parasitaemia was higher in the obese mice than in the control mice from day 3 to day 8 PI (Fig. 1-B), but the daily differences were never significant (0.98 > P > 0.07 by the Mann-Whitney U test), except at day 3 (0.017 parasitized erythrocytes per 100 erythrocytes in the control mice vs. 0.039 in the obese mice; P = 0.032). At day 18 PI, the mean body weight of the surviving ob/ob mice was 45.8 g.
The amount of injected sporozoites per mouse (50,000 or 100,000) had only transitory consequences on parasitaemias that were detectable only at days 3 and 4 PI (Table 1), indicating that doubling the sporozoite dose proportionally to the obese weight does not affect the observed responses in terms of mortality.
This study appears to provide the first indication that leptin-deficient ob/ob obese mice are permissive for the infection by Plasmodium berghei ANKA and resistant to cerebral malaria. This resistance cannot be explained solely by the larger size of these mice, as weight-adjusted doses of sporozoites were given. The protection of the obese mice with respect to cerebral malaria was unexpected, since they share the same C57BL/6 genetic background as the lean mice which are well known to be susceptible to cerebral malaria, usually dying from typical neurological symptoms between days 6–9 after infection . The susceptibility to cerebral malaria of this strain is confirmed by observations with the control lean heterozygote ob/+ mice.
Cerebral malaria induced by P. berghei ANKA is known to be under genetic regulation, but the mechanisms leading to protection versus death from cerebral malaria are not yet fully understood in normal non-obese mice . Using mice of different genetic backgrounds which are either cerebral malaria (CM) sensitive or CM resistant, and comparing gene expression profiling, Delahaye et al [19, 20] identified a large set of genes that discriminates between CM sensitive and resistant mice, including genes involved in regulating immune responses. Not surprisingly, the obese phenotype affects the expression of a large panel of genes, mainly involved in metabolic pathways possibly inducing resistance to CM [21, 22]. The leptin gene mutation in ob/ob is associated with observed protection against CM but, considering the complex and pleiotrophic effect of the leptin mutation in ob/ob mice, it is hard to speculate whether the unique mutation of the leptin gene would be totally responsible, per se, for the observed CM resistance phenotype in obese mice.
Several lines of evidences can be related to possible mechanisms involved in this protection. Among them are many related to the nutritional status and plasmatic lipid composition involved in immune responses. So the deep modifications of the lipid metabolism in ob/ob mice can have implications. Obese mice exhibit a hyperlipidemia caused by an increase in hepatic triglyceride synthesis and secretion . Interestingly, it has been demonstrated that injection of fatty acids during the first three days after infection protected C57BL/6 lean mice infected by P. berghei ANKA from cerebral symptoms . Regulation of glycemia is also affected in obese mice, resulting in hyperglycemia that may compensate the hypoglycemia commonly observed in severe or complicated malaria attacks. Elased et al  observed that infection with P. yoelii induces hypoglycemia in normal mice and normalizes the hyperglycemia in moderately diabetic mice. In contrast, blood glucose concentrations significantly rose in mice exhibiting neurological symptoms during infection with P. berghei ANKA .
On the other hand, ob/ob mice are leptin-deficient, and this could be one of the key factors involved in the observed resistance. Indeed, a progressive increase of leptin concentration was observed in P. berghei (Swiss Tropical Institute strain)-infected C57BL/6 lean mice, and the level of leptin increased five times when compared with non-infected control mice, six days after infection . Leptin is involved in protective mechanisms, allowing an organism to deal with the potentially auto-aggressive effects of its immune system . In other experiments, injections of leptin were claimed to protect against TNF toxicity in ob/ob but not in +/+ mice ; therefore, it is possible that leptin protects against cerebral malaria by damping down TNF-induced pathology. Similar in structure to interleukin 2, an important T-cell growth factor, leptin modifies proinflammatory immune responses and may provide a key link between nutritional deficiency and immune dysfunction. Obese ob/ob mice have impaired cell-mediated immunity and a propensity to develop Th2 rather than Th1 immune responses . A decrease in the Th1: Th2 ratio could be related to the observed resistance in obese mice because cerebral malaria due to P. berghei ANKA involves the Th1 cytokines TNF-α and INF-γ in the regulatory cascade controlling inflammatory responses after malarial infections .
Leptin can also regulate aspects of hemopoiesis, inducing proliferation, differentiation and functional activation of hemopoietic cells, but it has a weak impact on the bone-marrow nucleated erythroid cells . In other words, the severe anaemia observed in infected obese mice is a direct consequence of erythrocyte destruction by parasite proliferation, rather than leptin deficiency. Similar anaemia was observed in the few control mice that did not develop cerebral malaria.
While this study addressed the outcome of malaria in ob/ob mice, which are non-diabetic until the fourth week , it questions what would be the response of db/db diabetic mice, which also present obese phenotype. Indeed, drug-induced diabetic mice were shown to better control parasitaemia with enhanced phagocytic activity compared to normal mice . Apparent normalization of blood glucose concentration was also observed in a diabetic patient suffering from P. falciparum malaria . On the contrary, in mice exhibiting neurological symptoms during infection with P. berghei ANKA, blood glucose concentrations were significantly raised in line with TNF levels .
Beyond the malaria symptoms previously discussed, parasite density is worth considering. The data from this study showing higher parasitaemia in ob/ob mice during days 3–8 after infection are in agreement with what has been observed in obese fa/fa Zucker rats infected by P. yoelii yoelli presenting higher parasitaemias than heterozygous control rats. The higher and significant parasitaemia in ob/ob mice at day 3 questions a possible increased productivity of hepatic merozoites, as observed in single gene mutated Zucker rats .
It is noteworthy that mutations affecting leptin exist in humans, but with inconstant phenotypic manifestations in terms of obesity . Although experimental cerebral malaria models cannot reproduce all the features of human cerebral malaria, several observations in mice have been extended and confirmed in the human disease . They include immunological responses, blood-barrier function, histopathological features and expression of molecules in the brain and the retina, biochemical changes in the brain and behavioral changes. Events resulting in the development of human cerebral malaria complications are clearly multi-factorial, encompassing a dynamic interaction between sequestration, inflammation and homeostasis, in a complex syndrome leading to microcirculatory dysfunction [30, 38].
Control heterozygous mice infected with P. bergei ANKA developed rapid neurological syndromes leading to death, without high parasitaemia. By contrast, obese ob/ob mice did not develop cerebral malaria and presented an increasing parasitaemia resulting in severe anaemia. Although it is not possible to make inferences from mice to humans from these observations, the study highlights the need to recognize and address a possible link between obesity and its effect on the response to malarial infection in human populations.
This study received financial support from Institut de Recherche pour le Développement, Muséum National d'Histoire Naturelle, and Institut Pasteur. The critical comments of Serge Bonnefoy are gratefully acknowledged, as is mosquito production by Solange Touron. Fabrice Legros is also thanked.
- Prentice AM: The emerging epidemic of obesity in developing countries. Int J Epidemiol. 2006, 35: 93-99. 10.1093/ije/dyi272.View ArticlePubMedGoogle Scholar
- Amuna P, Zotor FB: Epidemiological and nutrition transition in developing countries: impact on human health and development. Proc Nutr Soc. 2008, 67 (1): 82-90. 10.1017/S0029665108006058.View ArticlePubMedGoogle Scholar
- Elased KM, De Souza JB, Playfair JH: Reversal of type 2 diabetes in mice by products of malaria parasites: I. Effect of inactivated parasites. Metabolism. 2000, 49: 937-941. 10.1053/meta.2000.6756.View ArticlePubMedGoogle Scholar
- Elased KM, Gumaa KA, De Souza JB, Rahmoune H, Playfair JH, Rademacher TW: Reversal of type 2 diabetes in mice by products of malaria parasites. II. Role of inositol phosphoglycans (IPGs). Mol Genet Metab. 2001, 73: 248-258. 10.1006/mgme.2001.3186.View ArticlePubMedGoogle Scholar
- Genton B, Al Yaman F, Ginny M, Taraika J, Alpers MP: Relation of anthropometry to malaria morbidity and immunity in Papua New Guinean children. Am J Clin Nutr. 1998, 68: 734-741.PubMedGoogle Scholar
- Tanner M, Burnier E, Mayombana C, Betschart B, de Savigny D, Marti HP, Suter R, Aellen M, Lüdin E, Degrémont AA: Longitudinal study on the health of children in a rural Tanzanian community: parasitoses and nutrition following control measures against intestinal parasites. Acta Tropica. 1987, 44: 137-174.PubMedGoogle Scholar
- Mbago MC, Namfua PP: Some determinants of nutritional status of one to four-year old children in low income urban areas in Tanzania. J Trop Pediatr. 1991, 38: 299-306.View ArticleGoogle Scholar
- Hendrickse RG, Hasan AH, Olumide LO, Akinkunmi A: Malaria in early childhood. An investigation of five hundred seriously ill children in whom a "clinical" diagnosis of malaria was made on admission to the children's emergency room at University College Hospital, Ibadan. Ann Trop Med Parasitol. 1971, 65: 1-20.PubMedGoogle Scholar
- Deen JL, Walraven GE, von Seidlein L: Increased risk for malaria in chronically malnourished children under 5 years of age in rural Gambia. J Trop Pediatr. 2002, 48: 78-83. 10.1093/tropej/48.2.78.View ArticlePubMedGoogle Scholar
- Onwuamaegbu ME, Henein M, Coats AJ: Cachexi in malaria and heart failure: therapeutic considerations in clinical practice. Postagrad Med J. 2004, 80: 642-649. 10.1136/pgmj.2004.020891.View ArticleGoogle Scholar
- Ariyasinghe A, Morshed SRM, Mannoor MK, Bakir HY, Kawamura H, Miyaji C, Nagura T, Kawamura T, Watanabe H, Sekikawa H, Abo T: Protection against malaria due to innate immunity enhanced by low-protein diet. J Parasitol. 2006, 92: 531-538. 10.1645/GE-551R1.1.View ArticlePubMedGoogle Scholar
- Sinnis P, Willnow TE, Briones MR, Herz J, Nussenzweig V: Remnant lipoproteins inhibit malaria sporozoite invasion of hepatocytes. J Exp Med. 1996, 184: 945-954. 10.1084/jem.184.3.945.View ArticlePubMedGoogle Scholar
- Schaible UE, Kaufmann SH: Malnutrition and infection: complex mechanisms and global impacts. PLoS Med. 2007, 4 (5): e115-10.1371/journal.pmed.0040115.PubMed CentralView ArticlePubMedGoogle Scholar
- Meister B: Control of food intake via leptin receptors in the hypothalamus. Vitam Horm. 2000, 59: 265-304.View ArticlePubMedGoogle Scholar
- Memon RA, Fuller J, Moser AH, Smith PJ, Grunfeld C, Feingold KR: Regulation of putative fatty acid transporters and Acyl-CoA synthetase in liver and adopise tissue in ob/ob mice. Diabetes. 1999, 48: 121-127. 10.2337/diabetes.48.1.121.View ArticlePubMedGoogle Scholar
- Lord GM: Leptin as a proinflkamatory cytokine. Contrib Nephrol. 2006, 151: 151-164.View ArticlePubMedGoogle Scholar
- Neill ALL, Hunt NH: Pathology of fatal and resolving Plasmodium berghei cerebral malaria in mice. Parasitology. 1992, 105: 165-175.View ArticlePubMedGoogle Scholar
- De Souza JB, Riley EM: Cerebral malaria: the contribution of studies in animal models to our understanding of immunopathogenesis. Microbes Infect. 2002, 4: 291-300. 10.1016/S1286-4579(02)01541-1.View ArticlePubMedGoogle Scholar
- Delahaye NF, Coltel N, Puthier D, Flori L, Houlgatte R, Iraqi FA, Nguyen C, Grau GE, Rihet P: Gene expression profiling discriminates between cerebral malaria (CM)-susceptible mice and CM-resistant mice. J Inf Dis. 2006, 193: 312-321. 10.1086/498579.View ArticleGoogle Scholar
- Delahaye NF, Coltel N, Puthier D, Barbier M, Benech P, Joly F, Iraqi FA, Grau GE, Nguyen C, Rihet P: Gene expression analysis reveals early changes in several molecular pathways in cerebral malaria-susceptible mice versus cerebral malaria-resistant mice. BMC Genomics. 2007, 8 (1): 452-10.1186/1471-2164-8-452.PubMed CentralView ArticlePubMedGoogle Scholar
- Liang CP, Tall AR: Transcriptional profiling reveals global defects in energy metabolism, lipoprotein, and bile acid synthesis and transport with reversal by leptin treatment in ob/ob mouse liver. J Biol Chem. 2001, 276 (52): 49066-49076. 10.1074/jbc.M107250200.View ArticlePubMedGoogle Scholar
- Lan H, Rabaglia ME, Stoehr JP, Nadler ST, Schueler KL, Zou F, Yandell BS, Attie AD: Gene expression profiles of nondiabetic and diabetic obese mice suggest a role of hepatic lipogenic capacity in diabetes susceptibility. Diabetes. 2003, 52: 688-700. 10.2337/diabetes.52.3.688.View ArticlePubMedGoogle Scholar
- Moumaris M, Sestier C, Miltgen F, Halbreich A, Gentilini M, Sabolovic D: Effect of fatty acid treatment in cerebral malaria-susceptible and nonsusceptible strains of mice. J Parasitol. 1995, 81: 997-999. 10.2307/3284055.View ArticlePubMedGoogle Scholar
- Elased K, Playfair JHL: Hypoglycemia and hyperinsulinemia in rodent models of severe malaria. Infect Immun. 1994, 62: 5157-5160.PubMed CentralPubMedGoogle Scholar
- Elased KM, Taverne J, Playfair JH: Malaria, blood glucose, and the role of tumour necrosis factor (TNF) in mice. Clin Exp Immunol. 1996, 105: 443-449. 10.1046/j.1365-2249.1996.d01-781.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Pulido-Mendez M, de Sanctis J, Rodriguez-Acosta A: Leptin and leptin receptors during malaria infection in mice. Folia Parasitol. 2002, 49: 249-251.View ArticlePubMedGoogle Scholar
- Matarese G, Leiter EH, La Cava A: Leptin in autoimmunity: many questions, some answers. Tissue Antigens. 2007, 70 (2): 87-95. 10.1111/j.1399-0039.2007.00886.x.View ArticlePubMedGoogle Scholar
- Takahashi N, Waelput W, Guisez Y: Leptin is an endogenous protective protein against the toxicity exerted by tumor necrosis factor. J Exp Med. 1999, 189: 207-212. 10.1084/jem.189.1.207.PubMed CentralView ArticlePubMedGoogle Scholar
- Tarsi RM, Cook HT, Jackson I, Pusey CD, Lord GM: Leptin-deficient mice are protected from accelerated nephrotoxic nephritis. Am J Pathol. 2004, 164: 385-390.View ArticleGoogle Scholar
- Lou J, Luca R, Grau GE: Pathogenesisis of cerebral malaria: recent experimental data and possible applications for humans. Clin Microbiol Rev. 2001, 14: 810-820. 10.1128/CMR.14.4.810-820.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Gainsford T, Willson TA, Metcalf D, Handman E, McFarlane C, Ng A, Nicola NA, Alexander WS, Hilton DJ: Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells. Proc Natl Acad Sci USA. 1996, 93 (25): 14564-14568. 10.1073/pnas.93.25.14564.PubMed CentralView ArticlePubMedGoogle Scholar
- Dubuc PU, Willis PL: Postweaning development of diabetes in ob/ob mice. Metabolism. 1979, 28 (6): 633-640. 10.1016/0026-0495(79)90016-7.View ArticlePubMedGoogle Scholar
- Elased K, De Souza JB, Playfair JH: Blood-stage malaria infection in diabetic mice. Clin Exp Immunol. 1995, 99: 440-444.PubMed CentralView ArticlePubMedGoogle Scholar
- Shalev O, Tsur A, Rahav G: Falciparum malaria-induced hypoglycaemia in a diabetic patient. Postgrad Med J. 1992, 68: 281-282.PubMed CentralView ArticlePubMedGoogle Scholar
- Lombard MN, Bazin R, Durand G, Beaugé F, Baccam D, Milgen F, Landau I: Rodent Plasmodium development in livers of genetically obese rats (fa/fa). Europ J Protist. 1998, 34: 78-81.View ArticleGoogle Scholar
- Lonnqvist F, Nordfors L, Schalling M: Leptin and its potential role in human obesity. J Intern Med. 1999, 245: 643-652. 10.1046/j.1365-2796.1999.00493.x.View ArticlePubMedGoogle Scholar
- Lamikanra AA, Brown D, Potocnik A, Casals-Pascual C, Langhorne J, Roberts DJ: Malarial anemia: of mice and men. Blood. 2007, 110: 18-28. 10.1182/blood-2006-09-018069.View ArticlePubMedGoogle Scholar
- Heyde van der HC, Nolan J, Combes V, Gramaglia I, Grau GE: A unified hypothesis for the genesis of cerebral malaria: sequestration, inflammation and hemostasis leading to microcirculatory dysfunction. Trends Parasitol. 2006, 22: 503-508. 10.1016/j.pt.2006.09.002.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.