- Case report
- Open Access
Atypical aetiology of a conjugal fever: autochthonous airport malaria between Paris and French Riviera: a case report
© Pomares-Estran et al; licensee BioMed Central Ltd. 2009
Received: 20 April 2009
Accepted: 23 August 2009
Published: 23 August 2009
Endemic malaria has been eradicated from France, but some falciparum malaria cases have been described in patients who have never travelled outside the country. Ms. V. 21 year-old and Mr. M. 23 year-old living together in Paris were on holiday in Saint Raphaël (French Riviera). They presented with fever, vertigo and nausea. A blood smear made to control thrombocytopaenia revealed intra-erythrocytic forms of Plasmodium falciparum. The parasitaemia level was 0.15% for Ms. V and 3.2% for Mr. M. This couple had no history of blood transfusion or intravenous drug use. They had never travelled outside metropolitan France, but had recently travelled around France: to Saint Mard (close to Paris Charles de Gaulle (CdG) airport), to Barneville plage (in Normandy) and finally to Saint Raphaël. The most probable hypothesis is an infection transmitted in Saint Mard by an imported anopheline mosquito at CdG airport. The DNA analysis of parasites from Ms. V.'s and Mr. M.'s blood revealed identical genotypes. Because it is unlikely that two different anopheline mosquitoes would be infected by exactly the same clones, the two infections must have been caused by the infective bites of the same infected mosquito.
In a recent paper, Doudier et al described two cases of possible autochthonous malaria contracted in Marseille area (southern France) during the summer 2006 . Malaria is a common disease in tropical and sub-tropical areas of over 100 countries, but endemic malaria has been eradicated from France. Nevertheless, some falciparum malaria cases have been described in patients who have never travelled outside France [1–6]. In this case presentation, two autochthonous malaria cases are reported in French Riviera during summer 2008.
Ms. V. 21 year-old and Mr. M. 23 year-old living together in Paris were on holiday in Saint Raphaël (French Riviera) since of August 13th, 2008. In the evening of August, 18th Ms. V. and Mr. M. presented with fever, vertigo and nausea (Day 0). The man's symptoms were more pronounced. He was hospitalized on August 19th in Fréjus-Saint Raphaël Hospital (Day 1). His platelet count was normal (Mr. M. = 208 giga/l). No aetiology was found. The patient went back home the next day with a follow-up but no diagnosis.
On August 22nd (Day 4), his symptoms were still present. The couple returned to the same hospital and a blood cell count, done at that time, found a thrombocytopaenia (Mr. M. = 11 giga/l, Ms. V. = 31 giga/l). A blood smear made to control the thrombocytopaenia and stained by May-Grünwald Giemsa revealed intra-erythrocytic forms of P. falciparum. The parasitaemia level was 0.15% for Ms. V and 3.2% for Mr. M. Retrospectively, the Day 1 blood smear of Mr. M. revealed P. falciparum with a parasitaemia level of 0.1%. They were hospitalized in Nice University Hospital (French Riviera) and treated with intravenous quinine because of vomiting. Parasitaemia controls done on Day 3 and Day 7 post treatment were negative and clinical evolution was successful.
Microsatellite loci genotyping
187, 195, 206, 214
133, 141, 148
187, 195, 206, 214
133, 141, 148
This couple had no history of blood transfusion or intravenous drug use. They had never travelled outside metropolitan France, however they had travelled around France in the previous fortnight: from the 5th to the 7th to Saint Mard, from the 7th to the 12th to Barneville plage (Normandy) and, finally, from the 13th to the 22nd in Saint Raphaël. Entomological and environmental studies have been conducted in Saint Raphaël and Saint Mard on the 28th of August and the 9th of September. No Anopheles sp. mosquito has been collected in these towns. The mean of temperature during their stay in Saint Raphaël and Saint Mard was 23°4 C (30°4 to 17°8 C) and 22°9 C (32°4 to 17°4 C) respectively. However, Saint Mard is 7 kilometers away from International Paris Charles de Gaulle (CdG) airport, where many airport-workers live. Furthermore, a hotel 300 metres away from the couple's home is served daily by a frequent shuttle from CdG Airport. The environmental and climatologic studies showed that there were no international port or airport in the area of Barneville plage and the mean temperature was quite low during their stay (18°5 C). Despite the compatible malaria incubation period no entomological study has been done in that place.
Autochthonous malaria is defined as Plasmodium sp. in blood smears of patients who have never travelled to malaria-endemic areas in the previous 12 months . Transfusion, organ transplantation, intravenous drug use, must also be excluded . The latest case of autochthonous malaria due to P. falciparum described in France was in 2006 . Among autochthonous malaria, airport malaria occurs when a Plasmodium-infected Anopheles sp. mosquito travels from an endemic area to a malaria-free airport . Two cases are described in a couple. The diagnosis was made incidentally on Day 4, when blood smears were done manually for thrombocytopaenia control. In France, it is legally defined that all thrombocytopaenia detected by the analyzer (platelet count below 150 giga/l) have to be controlled. A blood clot has to be excluded and a blood smear is done. On Day 1, in view to the lack of context (no travel history) and of thrombocytopaenia, the diagnosis was not made, but the disease was obviously still ongoing. DNA analysis revealed identical genotypes at all the investigated loci. Because it is unlikely that two different anopheline mosquitoes would be infected by exactly the same four clones, the present data shows that the two infections were caused by the infective bites of the same infected vector specimen, possibly during an interrupted blood feeding. In non-immune individuals most clinical manifestations occur between 7 and 14 days after the infective bite . According to this delay, the potential places of contamination are Saint Mard and Barneville plage and a contamination in Saint Raphäel is excluded. Moreover, 10 cases were described at CdG airport area during the summers 1994 and 1999 [6, 10]. In Europe, airport malaria occurred mainly in August when the climate in the vicinity of the origin and destination airport is suitable for the survival of malaria-carrying Anopheles . However, an autochthonous anopheline vector infected by a gametocyte carrier in Paris suburb or Barneville plage cannot be excluded . In continental France, nine Anopheles species are potential malaria vectors and among them Anopheles atroparvus is considered as the primary vector because of its abundance and its anthropophilous potential . Nevertheless, the temperature during their stay in Barneville plage is not in favour of a contamination in this location. In this case, because of incubation period, climate and environmental studies, the most probable hypothesis is an infection acquired during their stay in Saint Mard transmitted by an imported anopheline mosquito at CdG airport.
Written informed consent was obtained from the patient for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
The authors would like to thank Lionel Bertaux for his precious collaboration and Sandy Jones for English proof reading.
- Doudier B, Bogreau H, DeVries A, Ponçon N, Stauffer WM, Fontenille D, Rogier C, Parola P: Possible autochthonous malaria from Marseille to Minneapolis. Emerg Infect Dis. 2007, 13: 1236-1238.PubMed CentralView ArticlePubMedGoogle Scholar
- WHO regional Office for Europe: From malaria control to elimination in the WHO European region 2006-2015. [http://www.euro.who.int/document/e88840.pdf]
- Baixench MT, Suzzoni-Blatger J, Magnaval JF, Lareng MB, Larrouy G: Two cases of inexplicable autochthonous malaria in Toulouse, France. Med Trop. 1998, 58: 62-64.Google Scholar
- Marty P, Le Fichoux Y, Izri MA, Mora M, Mora M, Mathieu B, Vessaud P: Autochtonous Plasmodium falciparum malaria in southern France. Trans R Soc Trop Med Hyg. 1992, 86: 478-10.1016/0035-9203(92)90074-M.View ArticlePubMedGoogle Scholar
- Marty P, Delaunay P, Ribière C, Terrisse V, Bernard E, Le Fichoux Y, Dellamonica P: Domestic malaria in Nice (France) probably transmitted via the airport. Presse Med. 2001, 30: 488-PubMedGoogle Scholar
- Giacomini T, Mouchet J, Mathieu P, Petithory JC: Study of 6 cases of malaria acquired near Roissy-Charles-de-Gaulle in 1994. Necessary prevention measures in airports. Bull Acad Natl Med. 1995, 9: 35-351.Google Scholar
- Pradines B, Hovette P, Fusai T, Atanda HL, Baret E, Cheval P, Mosnier J, Callec A, Cren J, Amalvict R, Gardair JP, Rogier C: Prevalence of in vitro resistance to eleven standard or new antimalarial drugs among Plasmodium falciparum isolates from Pointe-Noire, Republic of the Congo. J Clin Microbiol. 2006, 44: 2404-2408. 10.1128/JCM.00623-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Savini H, Bogreau H, Bertaux L, Bouchiba H, Kraemer P, Parzy D, Garnotel E, Rogier C, Simon F, Pradines B: First case of emergence of atovaquone-proguanil resistance in Plasmodium falciparum during treatment in traveller in Comoros. Antimicrob Agents Chemother. 2008, 52: 2283-2284. 10.1128/AAC.00282-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Legros F, Arnaud A, El Mimouni B, Martin D, et le réseau de correspondants du CNREPIA: Paludisme d'importation en France métropolitaine: données épidémiologiques 2001-2004. Bull Epidémiol Hebdo. 2006, 32: 235-336.Google Scholar
- Lusina D, Legros F, Esteve V, Klerlein M, Giacomini T: Airport malaria: four new cases in suburban Paris during summer. Euro Surveill 1999. 2000, 5: 76-80.Google Scholar
- Collins WE, Jeffery GM: A retrospective examination of sporozoite- and trophozoite-induced infections with Plasmodium falciparum: development of parasitologic and clinical immunity during primary infection. Am J Trop Med Hyg. 1999, 61 (1 Suppl): 4-19.View ArticlePubMedGoogle Scholar
- Tatem AJ, Rogers DJ, Hay SI: Estimating the malaria risk of African mosquito movement by air travel. Malar J. 2006, 5: 57-10.1186/1475-2875-5-57.PubMed CentralView ArticlePubMedGoogle Scholar
- Karch S, Dellile MF, Guillet P, Mouchet J: African malaria vectors in European aircraft. Lancet. 2001, 357: 235-10.1016/S0140-6736(05)71339-8.View ArticlePubMedGoogle Scholar
- Schaffner F, Angel G, Geoffroy B, Hervy JP, Rhaeim A, Brunhes J: The mosquitoes of Europe. 2001, Paris: IRD editions and EID MéditerranéeGoogle Scholar
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