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Characterization of the Plasmodium vivax erythrocytic stage proteome and identification of a potent immunogenic antigen of the asexual stages
Malaria Journalvolume 9, Article number: P44 (2010)
With the genome of Plasmodium vivax sequenced , it would be important to determine proteomes of the parasite in order to assist efforts in understanding the basic biology of the parasite as well as provides the new tools for identifying novel antigens and drug targets.
Materials and methods
Lysates of P. vivax were separated by SDS-polycrylamide gel electrophoresis (SDS-PAGE) and proteins were identified by using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF/TOF) mass spectrometry. In addition, to identify proteins that might be recognized by host humoral immunity, we have separated them by two-dimensional gel electrophoresis. Proteins were then screened by western blot with immune serum. Spots that were recognized by the host serum were excised and identified by high-accuracy liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Several hundred proteins were confidently identified. All proteins were classified into functional classes (see Table 1). Four parasite proteins were recognized by P. vivax-immune human sera. Interestingly, one of the four proteins (PV180L), reacted with the convalescence sera, 6 months post treatment of P. vivax-immune donors (see Figure 1).
The PV180L encodes a 24.1 kDa hypothetical protein which expressed throughout the erythrocytic cycle of P. vivax and the antibodies to PV180L were long lasting. Therefore, more reports on the proteins of P. vivax parasite provide useful information and availability to facilitate not only basic research on this extraordinary malaria parasite, but also provide the new tools for drug and vaccine developments.
Carlton JM, Adams JH, Silva JC, Bidwell SL, Lorenzi H, Caler E: Comparative genomics of the neglected human malaria parasite Plasmo-dium vivax. Nature. 2008, 455 (7214): 757-763. 10.1038/nature07327.