Volume 9 Supplement 2

Parasite to Prevention: Advances in the understanding of malaria

Open Access

Natural infection of Plasmodium falciparum induces inhibitory antibodies to gametocyte development in human hosts

  • Natda Tonwong1, 2,
  • Jetsumon Sattabongkot3,
  • Jeeraphat Sirichaisinthop4 and
  • Rachanee Udomsangpetch1, 2
Malaria Journal20109(Suppl 2):P53

https://doi.org/10.1186/1475-2875-9-S2-P53

Published: 20 October 2010

Background

Gametocyte antigens of Plasmodium falciparum can induce immunity in patients from which further inhibits fertilization of gametes, and consequently oocyst production in the mosquito midgut [1]. Here, we determined naturally-induced antibodies from malaria patients in Thailand and clarified effect of the antibodies on gametocyte development. Sixty-one percent of P. falciparum-infected blood fed to female Anopheles mosquitoes showed no oocyst production. Twenty-six percent of these oocyst inhibitory plasma distorted morphology and hampered maturity of the gametocytes (Fig.1). A possible mechanism of the gametocyte inhibitory activity was shown by binding of the plasma antibodies to the live immature intraerythrocytic gametocytes during co-cultivation period.
Figure 1

The effects of human plasma from malaria patients on maturation of Plasmodium falciparum gametocytes. The data are shown in (A) mean percentages of gametocytes from stage I to V, (B) mean percentages of crisis form of gametocytes, and (C) number of asexual and sexual stage parasites in the cocultivation cultures. (a) malaria naive plasma, (b) oocyst non-inhibitory plasma, (c) oocyst inhibitory plasma, *P ≤ 0.05.

Conclusion

Anti-gametocyte antibodies were elicited during natural malaria infection. The oocyst inhibitory antibodies diffused to and interacted with developing intraerythrocytic gametocytes and reduced number of stage II to V gametocytes, and hampered their maturation. Therefore the alternative development of transmission blocking vaccine in the high transmission area should focus on the identification of the gametocyte antigens inducing inhibitory antibodies to reduce gametocytemia.

Authors’ Affiliations

(1)
Department of Pathobiology, Mahidol University
(2)
Department of Parasitology, Mahidol University
(3)
Department of Entomology, Armed Forces Research Institute of Medical Sciences
(4)
Vector Borne Disease Training Center, Pra Budhabat

References

  1. Bousema JT, Drakeley CJ, Sauerwein RW: Sexual stage antibody responses to P. falciparum in endemic populations. Curr Mol Med. 2006, 6: 223-229. 10.2174/156652406776055140.View ArticlePubMedGoogle Scholar

Copyright

© Tonwong et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Advertisement