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Falcipain 2 inhibitors and antiplasmodial compounds from a bio-guided fractionation of the fruits of Sorindeia juglandifolia A. Rich. (Anacardiaceae) growing in Cameroon

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Malaria Journal20109 (Suppl 2) :P6

  • Published:


  • Malaria
  • Plasmodium
  • Plasmodium Falciparum
  • Fresh Fruit
  • Antimalarial Activity


Discovering new lead compounds with the potential to become usable drugs against malaria is a crucial step to ensuring a sustainable global pipeline for innovative products. We describe here the results of an antimalarial activity-driven fractionation of the fruits of Sorindeia juglandifolia growing in Cameroon.

Materials and methods

Fresh fruits were collected by an ethnobotanist in Yaoundé area in May 2009. The plant was dried at Room Temperature during 7 days, powdered and extracted using organic solvents. The extract was fractionated by flash chromatography over silica gel (70-230 mesh, Merck, 7 x 42 cm), eluting with gradients of hexane-ethyl acetate mixtures, and resulted in 35 fractions, which were pooled on the basis of thin layer chromatography patterns. Resulting fractions were tested in vitro against the Plasmodium falciparum chloroquine-resistant strain W2, and the recombinant cysteine protease Falcipain 2 (F2) [1]. Two fractions showed the best potency and were selected for phytochemical investigation guided by biological activity.


The main end-compounds afforded through the phytochemical investigation were found to be known (Figure 1), 2,3,6-trihydroxy benzoic acid (1), and 2,3,6-trihydroxy methyl benzoate (2) that exhibited low micromolar inhibitory concentrations against P. falciparum W2 and Falcipain 2 respectively.
Figure 1
Figure 1

Antimalarial compounds isolated from Sorindeia juglandifolia.


The isolated compounds have not been previously investigated for antimalarial activity, and therefore suggesting further investigation.

Authors’ Affiliations

Laboratory of Phytobiochemistry, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, P.O., Box 812, Yaoundé, Cameroon
Laboratory for Public Health Biothechnology, Biotechnology Centre, UniversityofYaoundé 1, P.O. Box 8094, Yaoundé, Cameroon
Laboratory of Natural Products, Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon
Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, 94943, USA


  1. Boyom FF, Madiesse KEA, Tepongning R, Mbacham WF, Tsamo E, Amvam Zollo PH, Gut J, Rosenthal PJ: Antiplasmodial activity of extracts from seven medicinal plants used in malaria treatment in Cameroon. J Ethnopharmacol. 2009, 123: 483-488. 10.1016/j.jep.2009.03.008.View ArticlePubMedGoogle Scholar


© Boyom et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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