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Prime-boost strategy for vaccine development against both Plasmodium vivax and P. falciparum using MSP-119 as antigen

Background

Both antibodies and effector T cells appear to play important roles in the protection against P. vivax and P. falciparum infection. However, the partial protective immunity induced by vaccination with recombinant C-terminal region of merozoite surface protein (MSP-119) is mediated largely by antibodies ([1, 2]). Therefore, the objective of the present study was to evaluate, when PvMSP-119 and PfMSP-119 antigens were administered as combination at a single site in mice by using different immunization strategies (DNA/DNA, DNA/rprotein, rprotein/rprotein). We found that mice immunized with both recombinant antigens alone and in combination using heterologous prime-boost strategies (prime with DNA 100 ng and boost with recombinant protein 35 μg) lead to induced substantial levels of MSP-119 specif c IgG1, IgG2a and IgG2b (a mixed Th1/Th2 type) antibodies as measured by ELISA (Figure 1). In addition, both antigens significantly increased IFNγ responses in mice immunized with both antigens in combination using prime-boost strategy (Figure 2). rPfMSP-119 when combined with rPvMSP-119 was not affects antibodies or IFNγ and IL-10 responses in prime-boost strategy.

Figure 1
figure1

(abstract P64). Antibody responses to rPvMSP-119(a) and rPfMSP-119(b) in immunized mice.

Figure 2
figure2

(abstract P64). IFNγ responses to rPvMSP-119(a) and rPfMSP-119(b) in immunized mice.

Conclusion

The present results are encouraging to develop a multispecies human malaria vaccine against asexual blood stage of P. vivax and P. falciparum. Further study is needed to evaluate the protective efficacy of this vaccine in non-human primates.

References

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    Near KA, Stowers AW, Jankovic D, Kaslow DC: Improved immunogenicity and efficacy of the recombinant 19-kilodalton merozoite surface protein 1 by the addition of oligodeoxynucleotide and aluminium hydroxide gel in a murine malaria vaccine model. Infect Immun. 2002, 70: 692-701. 10.1128/IAI.70.2.692-701.2002.

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Correspondence to Sedigheh Zakeri.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mehrizi, A.A., Zakeri, S. & Djadid, N.D. Prime-boost strategy for vaccine development against both Plasmodium vivax and P. falciparum using MSP-119 as antigen. Malar J 9, P64 (2010). https://doi.org/10.1186/1475-2875-9-S2-P64

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Keywords

  • Malaria
  • Plasmodium
  • Protective Immunity
  • Vaccine Development
  • Substantial Level