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The influence of age on insecticide susceptibility of Anopheles arabiensis during dry and rainy seasons in rice irrigation schemes of Northern Tanzania
Malaria Journal volume 16, Article number: 364 (2017)
Insecticide resistance is the major emerging challenge facing the malaria vector control programmes in Tanzania. Proper monitoring and detection is of paramount importance guiding the vector control programmes. This paper presents the effect of mosquito aging on insecticide resistance status in Anopheles arabiensis populations in dry and rainy seasons in northern Tanzania.
Anopheles gambiae s.l. larvae were sampled from rice fields in both dry and rainy seasons and reared in the insectary to adults. The emerged females in batches of 2, 3, 5, and 10 days old were exposed to six insecticides (deltamethrin, permethrin, lambda-cyhalothrin, DDT, bendiocarb and pirimiphos-methyl) to see the effects of age on insecticide resistance. Mosquitoes were exposed to insecticides using WHO standard susceptibility test kits. Knockdown was recorded during the 1-h exposure, while mortality and resistance ratio were recorded 24 h later. Mosquito specimens were identified to species level using the polymerase chain reaction (PCR) method.
Among the 326 specimens processed by PCR, 323 (99.1%) were identified as Anopheles arabiensis. There was reduced mortality (ranging from 61 to 97.7%) when adults reared from larvae were exposed to all pyrethroids and bendiocarb in both dry and rainy seasons, while they were fully susceptible to DDT and pirimiphos-methyl. There was a significant increase in mortality rate with increase in mosquito’s age in both dry and rainy seasons following exposure to pyrethroids (DF = 1, P < 0.05). Mosquitoes showed significantly higher mortality rates in the rainy season than in the dry season after being exposed to pyrethroids (DF = 1, P < 0.05). Higher mortality rates (94.0–99.8%) were observed in all ages and seasons when mosquitoes were exposed to bendiocarb compared with pyrethroids. Pirimiphos-methyl was only tested in the rainy season so no comparison with dry season mosquitoes could be made.
Results showed that An. arabiensis were resistant to pyrethroids in both seasons and that the young age groups exhibited higher levels of resistance compared with the older age groups. Mosquitoes were full susceptible to DDT and pirimiphos-methyl irrespective of the season and age.
Malaria vector control mostly relies on indoor residual house spraying (IRS) and long-lasting insecticide-treated bed nets (LLINs)  and has accounted for the dramatic decline in malaria transmission over the past decade. Malaria control and subsequently elimination are the priority of sub-Saharan African countries through national malaria control programmes and other donors . In Tanzania, wide coverage and use of LLINS began in 2005 and has been a sustainable exercise to date with aid from different funders [3,4,5].
Insecticide resistance in Tanzania has been reported widely in the major malaria vectors Anopheles gambiae s.s., Anopheles arabiensis and Anopheles funestus [6,7,8] causing concern for the vector control programmes. Only four classes of insecticides are approved for use in vector control: pyrethroids (the only class used for LLIN treatment), organophosphates, carbamates and organochlorides . Pyrethroids is the only class of insecticides widely used in sub-Saharan Africa for both LLINs and IRS and the rapidly spreading resistance to these insecticides is a challenge and major drawback to the gains achieved in malaria control to date [10,11,12]. Organophosphates and carbamates have been shown to have higher efficacy than pyrethroids for IRS [13,14,15,16]. However, in some areas of Tanzania there is evidence that mosquitoes populations are resistant to carbamate [14, 17]. The need for new insecticide classes is of paramount importance for handling and managing vector resistance .
In Tanzania, insecticide resistance is linked with agricultural activities [19,20,21], use of LLINs and IRS programmes with wide coverage [14, 22]. The expanding irrigation programmes for food security and cash crops cultivation such as cotton have increased the use of pesticides which subsequently is associated with resistance build-up in mosquitoes [7, 23]. The observed resistance is closely related to the distribution of the An. gambiae species complex and different mechanisms associated with insecticide tolerance [6,7,8, 12]. Both kdr mutations, L1014S and L1014F, have been found to occur in both An. arabiensis and An. gambiae s.s. in different populations in different parts of Tanzania . The L1014F mutation reduces sensitivity to both pyrethroids and DDT .
It is anticipated that, in areas with intense insecticide resistance, the survivorship of vector mosquitoes will be higher despite the use of LLINs and IRS . The association between insect fitness and insecticide tolerance may decrease or increase the vectorial capacity of mosquitoes . In general, mosquitoes lose their insecticide tolerance as age increases . The effectiveness of LLINs and IRS is due to their efficiency in reducing daily survivorship of vector mosquitoes and for LLINs, producing a physical barrier between the mosquito and its host. If insecticide tolerance decreases with aging, then the intervention tools will continue offering the maximum protection. Furthermore, there is no evidence on the effect of seasonality on the susceptibility status of mosquito populations of different ages in Tanzania.
The objectives of this study, therefore, were to determine aging and seasonality effects on insecticide resistance status of wild populations of Anopheles gambiae s.l. in northern Tanzania.
This study was conducted at the Lower Moshi rice irrigation scheme in Mabogini village situated at the foot of mount Kilimanjaro (37°20′E, 03°21′S: 750 m above sea level). The area is characterized by a tropical climate with heavy rains from March to May (18–27 °C) and a short rainy season during October to December (17–28.5 °C). A hot dry season occurs from January to February (17–30 °C) while the cold dry season is from June to September (13.7–24.8 °C) .
Collection and rearing of wild An. gambiae s.l. larvae
Larvae of different life stages (L1–L4) of An. gambiae s.l. were collected within rice farms at Mabogini during the dry season (July–September 2013) and the rainy season (March–May 2014). The Lower Moshi rice schemes are irrigated throughout the year and ensure continuous breeding sites for mosquitoes. In the laboratory, the collected larvae were re-distributed evenly in development trays with habitat water, and provided with Nestle Cerelac mixed with fish powder (ratio 3:1) once a day. Larvae were reared to adults under insectary conditions of 27 ± 2 °C and 78 ± 2% relative humidity at TPRI, Mabogini field station with photophase of 12L:12D.
Insecticide susceptibility tests
The Kisumu susceptible laboratory strain was used as a positive control for all exposures to ensure that the test papers were effective. To determine the influence of age on insecticide resistance status, non blood-fed emergent adult females were held for 2, 3, 5, and 10 days after emergence before exposure to insecticides. The age structure used was based on previous studies used that age as the determinant of resistance limit [28,29,30]. Standard WHO susceptibility test kits were used. Six insecticides were tested: deltamethrin (0.05%), permethrin (0.75%), lambda-cyhalothrin (0.05%), bendiocarb (0.1%), DDT (4.0%) and pirimiphos-methyl (0.025%). Only female An. gambiae s.l. were used for the susceptibility tests according to WHO criteria . Each complete bioassay was performed with six batches of 20 unfed females of the same age. Four batches were exposed to treated filter papers, and two batches exposed to oil treated filter papers (negative control). The numbers of mosquitoes knocked down after contact with insecticides were recorded at the recommended intervals . The exposure period was 1 h and mortality was determined 24 h post-exposure. After being exposed to insecticide for 1 h, mosquitoes were provided with a 10% sugar solution for 24 h before the mortality score.
All specimens were identified to species using the standard DNA PCR method for the An. gambiae complex . DNA samples were extracted from a single leg or wing and processed according to the protocol of Scot et al. .
Data were entered in MS-excel and transferred to PASW Statistics version 18.0 for Windows (SPSS Inc., Chicago, IL) for analysis. Regression probit analysis was deployed to calculate the KDT50 and KDT95 of the population by season, insecticide type and mosquito age. Percent mortalities were used to determine the insecticide susceptibility/resistance status of the population during both dry and rainy seasons using one way Anova with Tukeys HSD test to separate the significance difference between the means. The field-collected mosquitoes KDT50 was compared with that of the Anopheles gambiae, Kisumu susceptible strain by estimates of KDT50 ratios (Resistance Ratio). The significant levels were considered at 5% and less.
Species composition in study site
A total of 326 specimens of An. gambiae s.l. were processed by PCR and 323 (99.1%) were identified as An. arabiensis (Fig. 1). Three specimens did not amplify.
Insecticide susceptibility for wild and laboratory Kisumu strain populations
The knock down times for 50 and 95% (KDT50 and KDT95) of the wild and laboratory colony population varied with insecticide type. For DDT, the mean KDT50 and mean KDT95 were higher in the rainy season than in the dry season for all ages (Table 1). For lambda-cyhalothrin, the rainy season had higher KDT50 and KDT95 at the age of 2, 3 and 5 days while for day 10, KDT50 and KDT95 was higher in the dry season than the rainy season (Table 2). For Deltamethrin, at the age of 2 and 5 days KDT50 and KDT95 was higher in the dry season than in the rainy season while for mosquitoes of 3 and 10 days old, KDT50 and KDT95 was higher in the rainy season than in the dry season (Table 3). For Permethrin, at the age of 2, 3 and 5 days old, KDT50 and KDT95 was higher in the dry season than the rainy season and alternated at the age of 10 days, with higher KDT50 in the rainy season and higher KDT95 in the dry season (Table 4). For Bendiocarb, KDT50 and KDT95 for the age of 2,3, and 5 days old was higher in the dry season than the rainy season while at 10 days old the KDT50 and KDT95 was higher in the rainy season than the dry season (Table 5). For pirimiphos-methyl, KDT50 and KDT95 for the age of 2, 3 5 and 10 days in the rainy season increased with aging (Table 6). The Kisumu An. gambiae susceptible colony was used as controls for KDT50 and KDT95 for each season and each insecticide (Tables 1, 2, 3, 3, 5 and 6).
The KDT50 of the wild populations were compared with those for the Kisumu strain (2 days old) to obtain the resistance ratio (RR) for each age group and each insecticide. The results are presented in Tables 1, 2, 3, 3, 5 and 6.
Age structure, seasonality and resistance
There was significant difference in mortality depending on the age of adults tested and insecticide used. Mortality was age dependant for all pyrethroids with more young mosquitoes surviving than the older age groups in both dry and rainy seasons (Fig. 2). There was significant difference in mortality between the four age structures tested with pyrethroids in both seasons (DF = 2, P < 0.001) but no difference between the two seasons (DF = 1, P > 0.05) (Tables 2, 3 and 4). For bendiocarb, mortality ranged between 94 and 99.7% in both seasons, and there was no significance difference in mortality between the age groups and the season (DF = 1, P > 0.05). Full susceptibility (100% mortality) was observed when all age groups of An. arabiensis were exposed to DDT in both seasons and pirimiphos-methyl in the rainy season (Fig. 2).
The findings of this study have shown that the wild population of An. arabiensis from Mabogini exhibits different levels of mortality to the three pyrethroids tested depending on the age of the mosquitoes. These results are similar to previous studies that found mortality to be age dependant but did not include seasonality as a variable [26, 28, 32]. The higher resistance of the young mosquitoes to pyrethroids could be due to their strength and physiological activeness compared to older ones [28, 32]. This is because mosquitoes may lose energy due to aging, which is also needed for resistance mechanisms, thus mosquitoes reduce their ability to adapt to environmental stress . Similar observations were reported from Côte d’lvoire with increased mortality associated with mosquito age following exposure to deltamethrin and permethrin [28, 32]. The increase of mortality to pyrethroids with increase in mosquito age has been found in other malaria vector species, including Anopheles sinensis  and An. funestus [34, 35].
Mosquitoes exhibited higher resistance to pyrethroids in the dry season than in the rainy season but they were not statistically different in some age groups, in deltamethrin statistical significant variation between rainy and dry seasons was observed in 10 days old mosquitoes (Table 3), for lambdacyalothrin significant difference between rainy and dry seasons was only in age of 2 and 3 days old (Table 2). In permethrin all ages (2, 3, 5 and 10 days old) had significant variation between seasons (Table 4). This might possibly be attributed to high concentrations of insecticides in the rice fields where larvae were collected, compared to the rainy season when insecticide concentration would be diluted by the rains, though further investigation is needed to explore this. The higher mortality rates in adult An. arabiensis exposed to bendiocarb and DDT in both seasons might be due to restricted use of these insecticides , and likewise for pirimiphos-methyl which was only tested during rainy season. Similar results were observed for three years in wild populations of An. funestus and An. arabiensis in Malawi between 2011 and 2015 .
The seasonal variations in the mortality rates on pyrethroids (deltamethrin, permethrin and lambda-cyhalothrin) might possibly be due to the fluctuation of environmental factors such as temperature and humidity. In previous studies it was found that temperature and humidity have a significant role in influencing the susceptibility of mosquitoes to different insecticides . The observed elevated resistance to Bendiocarb was suggested to be due to increased metabolic activities of the enzymes [15, 16].
Susceptibility of An. arabiensis to pirimiphos-methyl and DDT is probably due to the limited use of these insecticides for malaria control and hence mosquitoes are not exposed to them. Susceptibility of An. gambiae s.l. to pirimiphos-methyl was also observed in Benin  and in An. funestus in Zambia and Zimbabwe . But DDT susceptibility can also be due to the underlying mechanism for resistance in the study area which is detoxification by the enzyme Glutathione-s-transferases (GST) which was reported previously .
The current study has confirmed that An. arabiensis is more resistant to pyrethroids than other insecticides in both the dry and rainy seasons, and the younger age groups exhibited higher levels of resistance than older age groups. Mosquitoes were fully susceptible to DDT and Pirimiphos-methyl in irrespective of the season and age. These results should be taken into account by malaria vector control stakeholders when considering the selection of appropriate insecticides for resistance management strategies.
indoor residual spray
- KDT50 :
time taken for 50% of the sample to be knocked down
- KDT95 :
time taken for 95% of the sample to be knocked down
long lasting insecticide-treated bed nets
polymerase chain reaction
World Health Organization
World Health Organization. World Malaria report 2015. Geneva: World Health Organization; 2015.
Simon J, Yeboah-Antwi K, Schapira A, Cham MK, Barber-Madden R, Brooks MI. External evaluation of the Presidents Malaria Initiative. Final report. 2011.
Bonner K, Mwita A, McElroy P, Omari S, Mzava A, Lengeler C, et al. Design, implementation and evaluation of a national campaign to distribute nine million free LLINs to children under five years of age in Tanzania. Malar J. 2011;10:73.
Magesa S, Lengeler C, deSavigny D, Miller J, Njau R, Kramer K, et al. Creating an “enabling environment” for taking insecticide treated nets to national scale: the Tanzanian experience. Malar J. 2005;4:34.
West P, Protopopoff N, Rowland M, Kirby M, Oxborough R, Mosha F, et al. Evaluation of a national universal coverage campaign of long-lasting insecticidal nets in a rural district in north-west Tanzania. Malar J. 2012;11:273.
Kabula B, Kisinza W, Tungu P, Ndege C, Batengana B, Kollo D, et al. Co-occurrence and distribution of East (L1014S) and West (L1014F) African knock-down resistance in Anopheles gambiae sensu lato population of Tanzania. Trop Med Int Health. 2014;19:331–41.
Kabula B, Tungu P, Malima R, Rowland M, Minja J, Wililo R, et al. Distribution and spread of pyrethroid and DDT resistance among the Anopheles gambiae complex in Tanzania. Med Vet Entomol. 2014;28:244–52.
Matowo J, Jones C, Kabula B, Ranson H, Steen K, Mosha F, et al. Genetic basis of pyrethroid resistance in a population of Anopheles arabiensis, the primary malaria vector in Lower Moshi, north-eastern Tanzania. Parasit Vectors. 2014;7:274.
World Health Organization. Test procedures for insecticide resistance monitoring in malaria vector mosquitoes. Geneva: World Health Organization; 2013.
Gnanguenon V, Agossa F, Badirou K, Govoetchan R, Anagonou R, Oke-Agbo F, et al. Malaria vectors resistance to insecticides in Benin: current trends and mechanisms involved. Parasit Vectors. 2015;8:223.
Mulamba C, Riveron JM, Ibrahim SS, Irving H, Barnes KG, Mukwaya LG, et al. Widespread pyrethroid and DDT resistance in the major malaria vector Anopheles funestus in East Africa is driven by metabolic resistance mechanisms. PLoS ONE. 2014;9:e110058.
Jones C, Haji K, Khatib B, Bagi J, Mcha J, Devine G, et al. The dynamics of pyrethroid resistance in Anopheles arabiensis from Zanzibar and an assessment of the underlying genetic basis. Parasit Vectors. 2013;6:343.
Silva APB, Santos JMM, Martins AJ. Mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids – a review. Parasit Vectors. 2014;7:450.
Matowo J, Kitau J, Kaaya R, Kavishe R, Wright A, Kisinza W, et al. Trends in the selection of insecticide resistance in Anopheles gambiae s.l. mosquitoes in northwest Tanzania during a community randomized trial of longlasting insecticidal nets and indoor residual spraying. Med Vet Entomol. 2015;29:51–9.
Aikpon R, Sezonlin M, Osse R, Akogbeto M. Evidence of multiple mechanisms providing carbamate and organophosphate resistance in field An. gambiae population from Atacora in Benin. Parasit Vectors. 2014;7:568.
Assogba B, Djogbenou L, Saizonou J, Milesi P, Djossou L, Djegbe I, et al. Phenotypic effects of concomitant insensitive acetylcholinesterase (ace-1R) and knockdown resistance (kdrR) in Anopheles gambiae: a hindrance for insecticide resistance management for malaria vector control. Parasit Vectors. 2014;7:548.
Protopopoff N, Matowo J, Malima R, Kavishe R, Kaaya R, Wright A, et al. High level of resistance in the mosquito Anopheles gambiae to pyrethroid insecticides and reduced susceptibility to bendiocarb in north-western Tanzania. Malar J. 2013;12:149.
Kelly-Hope L, Ranson H, Hemingway J. Lessons from the past: managing insecticide resistance in malaria control and eradication programmes. Lancet Infect Dis. 2008;8:387–9.
Mahande AM, Dusfour I, Matias JR, Kweka EJ. Knockdown resistance, rdl alleles, and the annual entomological inoculation rate of wild mosquito populations from Lower Moshi, Northern Tanzania. J Glob Infect Dis. 2012;4:114–9.
Nkya T, Akhouayri I, Poupardin R, Batengana B, Mosha F, Magesa S, et al. Insecticide resistance mechanisms associated with different environments in the malaria vector Anopheles gambiae: a case study in Tanzania. Malar J. 2014;13:28.
Nkya TE, Akhouayri I, Kisinza W, David J-P. Impact of environment on mosquito response to pyrethroid insecticides: facts, evidences and prospects. Insect Biochem Mol Biol. 2013;43:407–16.
Okumu F, Mbeyela E, Lingamba G, Moore J, Ntamatungiro A, Kavishe D, et al. Comparative field evaluation of combinations of long-lasting insecticide treated nets and indoor residual spraying, relative to either method alone, for malaria prevention in an area where the main vector is Anopheles arabiensis. Parasit Vectors. 2013;6:46.
Dabiré KR, Diabaté A, Namontougou M, Djogbenou L, Kengne P, Simard F, et al. Distribution of insensitive acetylcholinesterase (ace-1R) in Anopheles gambiae s.l. populations from Burkina Faso (West Africa). Trop Med Int Health. 2009;14:396–403.
Yewhalaw D, Kweka EJ. Insecticide resistance in East Africa—history, distribution and drawbacks on malaria vectors and disease control. In: Trdan S, editor. Insecticides Resistance. Rijeka: Intech; 2016. p. 189–215.
Rivero A, Vézilier J, Weill M, Read AF, Gandon S. Insecticide control of vector-borne diseases: when is insecticide resistance a problem? PLoS Pathog. 2010;6:e1001000.
Rajatileka S, Burhani J, Ranson H. Mosquito age and susceptibility to insecticides. Trans R Soc Trop Med Hyg. 2011;105:247–53.
Moshi monthly climate average. https://www.worldweatheronline.com/moshi-weather-averages/kilimanjaro/tz.aspx. Accessed 4 Nov 2016.
Chouaibou MS, Chabi J, Bingham GV, Knox TB, N’Dri L, Kesse NB, et al. Increase in susceptibility to insecticides with aging of wild Anopheles gambiae mosquitoes from Côte d’Ivoire. BMC Infect Dis. 2012;12:214.
Sikulu MT, Majambere S, Khatib BO, Ali AS, Hugo LE, Dowell FE. Using a near-infrared spectrometer to estimate the age of Anopheles mosquitoes exposed to pyrethroids. PLoS ONE. 2014;9:e90657.
Seaman JA, Alout H, Meyers JI, Stenglein MD, Dabiré RK, Lozano-Fuentes S, et al. Age and prior blood feeding of Anopheles gambiae influences their susceptibility and gene expression patterns to ivermectin-containing blood meals. BMC Genom. 2015;16:797.
Scott JA, Brogdon WG, Collins FH. Identification of single specimens of the Anopheles gambiae complex by the Polymerase chain reaction. Am J Trop Med Hyg. 1993;49:520–9.
Jones CM, Sanou A, Guelbeogo WM, Sagnon NF, Johnson PC, Ranson H. Aging partially restores the efficacy of malaria vector control in insecticide-resistant populations of Anopheles gambiae s.l. from Burkina Faso. Malar J. 2012;11:24.
Xu T, Zhong D, Tang L, Chang X, Fu F, Yan G, et al. Anopheles sinensis mosquito insecticide resistance: comparison of three mosquito sample collection and preparation methods and mosquito age in resistance measurements. Parasit Vectors. 2014;7:54.
Hunt RH, Brooke BD, Pillay C, Koekemoer LL, Coetzee M. Laboratory selection for and characteristics of pyrethroid resistance in the malaria vector Anopheles funestus. Med Vet Entomol. 2005;19:271–5.
Christian R, Matambo T, Spillings B, Brooke B, Coetzee M, Koekemoer L. Age-related pyrethroid resistance is not a function of P450 gene expression in the major African malaria vector, Anopheles funestus (Diptera: Culicidae). Genet Mol Res. 2011;10:3220–9.
Mzilahowa T, Chiumia M, Mbewe RB, Uzalili VT, Luka-Banda M, Kutengule A, et al. Increasing insecticide resistance in Anopheles funestus and Anopheles arabiensis in Malawi, 2011–2015. Malar J. 2016;15:563.
Choi KS, Christian R, Nardini L, Wood OR, Agubuzo E, Muleba M, et al. Insecticide resistance and role in malaria transmission of Anopheles funestus populations from Zambia and Zimbabwe. Parasit Vectors. 2014;7:464.
SM, GN, RP and EJK conceived the study; SM, EAM and AMM designed and coordinated the field collections, bioassays and laboratory processing; SM and EJK performed the statistical analysis; GN, SM, RP MC and EJK drafted the manuscript. All authors read and approved the final manuscript.
Authors acknowledge contributions of Augustine Mtui, Adrian Massawe and Ibrahim Sungi for their valuable efforts to support field larvae sampling and laboratory trials with the materials necessary for the implementation of the project. Authors appreciate village members whose rice fields were used for mosquito collections.
The authors declare that they have no competing interests.
This study had financial support from the South African NRF and Tanzanian COSTECH Grant No. TZ-RSA/JRP/RG.2013.08 awarded to EJK and MC for the field and laboratory based activities.
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