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Fig. 3 | Malaria Journal

Fig. 3

From: Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood

Fig. 3

Identification and propagation of Plasmodium vivax AMP2014.01-infected human RBCs by flow cytometry. ac The human glycophorin A (GPA)-specific monoclonal antibody (CD235a) was used to differentiate human RBCs from Saimiri RBCs separately and in mixed cultures. For experiments in a, b host RBCs were not exposed to AMP2014.01; infected Saimiri RBCs were introduced in the experiment shown in c. Assessment of FSC (Y-axis) in all panels further defines gating parameters of GPA− and GPA+ parasitized RBCs. df AMP2014.01-infected Saimiri RBCs were mixed with fresh human RBCs and their GPA+/DNA+ cells were identified (top right corner) at time 0, 72, and 144 h. Results show only those human GPA+ RBCs appearing within gating defined in ac. All controls and experiments were run in triplicate

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