Fig. 3

Identification and propagation of Plasmodium vivax AMP2014.01-infected human RBCs by flow cytometry. a–c The human glycophorin A (GPA)-specific monoclonal antibody (CD235a) was used to differentiate human RBCs from Saimiri RBCs separately and in mixed cultures. For experiments in a, b host RBCs were not exposed to AMP2014.01; infected Saimiri RBCs were introduced in the experiment shown in c. Assessment of FSC (Y-axis) in all panels further defines gating parameters of GPA− and GPA+ parasitized RBCs. d–f AMP2014.01-infected Saimiri RBCs were mixed with fresh human RBCs and their GPA+/DNA+ cells were identified (top right corner) at time 0, 72, and 144 h. Results show only those human GPA+ RBCs appearing within gating defined in a–c. All controls and experiments were run in triplicate