Fig. 1

Selecting and expressing pvdbp gene fragments. The amplification of binding (pvdbp-rii: 553–1596 bp) and non-binding regions (pvdbp-riii/v: bp 1792–2487) encoding rPvDBP-RII (aa 185–532) and rPvDBP-RIII/V (aa 598–829). PvDBP minimal reticulocyte binding regions are shown in dark grey; a region having normocyte and reticulocyte binding activity is shown in light grey. Agarose gel: lane 1 is the molecular weight marker. Lane 2 indicates PCR amplification of pvdbp-rii (1047 bp) and pvdbp-riii/v (699 bp) fragments. Western blot and SDS-PAGE: the molecular weight marker is shown (MW kDa), as are the molecules extracted from the soluble fraction (S) and/or inclusion bodies (IB) and purified (P)