WHO. World Malaria Report 2021. Geneva: World Health Organization; 2021.
Google Scholar
Roux AT, Maharaj L, Oyegoke O, Akoniyon OP, Adeleke MA, Maharaj R, et al. Chloroquine and sulfadoxine–pyrimethamine resistance in sub-Saharan Africa—a review. Front Genet. 2021;12:668574.
Article
CAS
PubMed
PubMed Central
Google Scholar
Fidock DA, Nomura T, Talley AK, Cooper RA, Dzekunov SM, Ferdig MT, et al. Mutations in the P. falciparum digestive vacuole transmembrane protein Pfcrt and evidence for their role in chloroquine resistance. Mol Cell. 2000;6:861–71.
Article
CAS
PubMed
PubMed Central
Google Scholar
Djimde A, Pharm D, Ogobara K, Doumbo MD, Joseph F, et al. A molecular marker for chloroquine resistant falciparum malaria. N Ingl J Med. 2001;344:257–63.
Article
CAS
Google Scholar
Ibrahim ML, Steenkste N, Khim N, Adam HH, Konate L, Coppee JY, et al. Field-based evidence of fast and global increase of Plasmodium falciparum drug resistance by DNA-microarrays and PCR/RFLP in Niger. Malar J. 2009;8:32.
Article
PubMed
PubMed Central
Google Scholar
Golassa L, Kamugisha E, Ishengoma DS, Baraka V, Shayo A, Baliraine FN, et al. Identification of large variation in Pfcrt, Pfmdr-1 and Pfubp-1 markers in Plasmodium falciparum isolates from Ethiopia and Tanzania. Malar J. 2015;14:264.
Article
PubMed
PubMed Central
Google Scholar
Ikegbunam MN, Nkonganyi CN, Thomas BN, Esimone CO, Velavan TP, Ojurongbe O. Analysis of Plasmodium falciparum Pfcrt and Pfmdr1 genes in parasite isolates from asymptomatic individuals in Southeast Nigeria 11 years after withdrawal of chloroquine. Malar J. 2019;18:343.
Article
PubMed
PubMed Central
Google Scholar
Shrivastava SK, Gupta RK, Mahanta J, Dubey ML. Correlation of molecular markers, Pfmdr1-N86Y and Pfcrt-K76T, with in vitro chloroquine resistant Plasmodium falciparum, isolated in the malaria endemic states of Assam and Arunachal Pradesh, Northeast India. PLoS ONE. 2014;9:e103848.
Article
PubMed
PubMed Central
Google Scholar
Veiga MI, Dhingra SK, Henrich PP, Straimer J, Gna N, Uhlemann A, et al. Globally prevalent PfMDR1 mutations modulate Plasmodium falciparum susceptibility to artemisinin-based combination therapies. Nat Commun. 2016;7:11553.
Article
CAS
PubMed
PubMed Central
Google Scholar
Singh G, Singh R, Urhekar AD, Rane K. Gene sequence polymorphisms mutations in Pfmdr-1 and Pfcrt-o genes of Plasmodium falciparum. Int J Curr Microbiol Appl Sci. 2016;5:451–8.
Article
CAS
Google Scholar
Cui L, Mharakurwa S, Ndiaye D, Rathod PK, Rosenthal PJ. Antimalarial drug resistance: literature review and activities and findings of the ICEMR Network. Am J Trop Med Hyg. 2015;93:57–68.
Article
CAS
PubMed
PubMed Central
Google Scholar
Tulu AN, Webbeg RH, Schellenberg AJS, Bradley DJ. Failure of chloroquine treatment for malaria in the highlands of Ethiopia. Trans R Soc Trop Med Hyg. 1996;44:556–7.
Article
Google Scholar
Hailemeskel E, Menberu T, Shumie G, Behaksra S, Chali W, Keffale M, et al. Prevalence of Plasmodium falciparum Pfcrt and Pfmdr1 alleles in settings with different levels of Plasmodium vivax co-endemicity in Ethiopia. Int J Parasitol Drugs Drug Resist. 2019;11:8–12.
Article
PubMed
PubMed Central
Google Scholar
Heuchert A, Abduselam N, Zeynudin A, Eshetu T, Loscher T, Wieser A, et al. Molecular markers of anti-malarial drug resistance in Southwest Ethiopia over time: regional surveillance from 2006 to 2013. Malar J. 2015;14:208.
Article
PubMed
PubMed Central
Google Scholar
Mekonnen SK, Aseffa A, Berhe N, Teklehaymanot T, Clouse RM, Gebru T, et al. Return of chloroquine-sensitive Plasmodium falciparum parasites and emergence of chloroquine-resistant Plasmodium vivax in Ethiopia. Malar J. 2014;13:244.
Article
PubMed
PubMed Central
Google Scholar
Kublin JG, Cortese JF, Njunju EM, Mukadam RAG, Wirima JJ, Kazembe PN, et al. Reemergence of chloroquine-sensitive Plasmodium falciparum malaria after cessation of chloroquine use in Malawi. J Infect Dis. 2003;187:1870–5.
Article
PubMed
Google Scholar
Ketema T, Bacha K, Getahun K, Del Portillo HA, Bassat Q. Plasmodium vivax epidemiology in Ethiopia 2000–2020: a systematic review and meta-analysis. PLoS Negl Trop Dis. 2021;15:e0009781.
Article
PubMed
PubMed Central
Google Scholar
Golassa L, White MT. Population – level estimates of the proportion of Plasmodium vivax blood-stage infections attributable to relapses among febrile patients attending Adama Malaria Diagnostic Centre. Malar J. 2017;16:307.
Article
Google Scholar
File T, Chekol T, Solomon G, Dinka H, Golassa L. Detection of high frequency of MAD20 allelic variants of Plasmodium falciparum merozoite surface protein 1 gene from Adama and its surroundings, Oromia, Ethiopia. Malar J. 2021;20:385.
Article
CAS
PubMed
PubMed Central
Google Scholar
WHO. Basic Malaria Microscopy. Part I. Learner’s guide, 2nd Edn. Geneva: World Health Organization; 2010.
Nega D, Daniel D, Tefera T, Eshetu T. Prevalence and predictors of asymptomatic malaria parasitemia among pregnant women in the rural surroundings of Arbaminch town. PLoS ONE. 2015;10:e0123630.
Article
PubMed
PubMed Central
Google Scholar
Atrosh WM, Al-Mekhalfi HM, Mahdy MAK, Surin J. The detection of Pfcrt and Pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia. Malar J. 2012;11:251.
Article
Google Scholar
Mulenga MC, Sitali L, Ciubotariu II, Hawela MB, Hamainza B. Decreased prevalence of the Plasmodium falciparum Pfcrt K76T and Pfmdr1 N86Y mutations post-chloroquine treatment withdrawal in Katete District, Eastern Zambia. Malar J. 2021;20:329.
Article
CAS
PubMed
PubMed Central
Google Scholar
Fontecha G, Pinto A, Archaga O, Betancourth S, Escober L, Henríquez J, et al. Assessment of Plasmodium falciparum anti-malarial drug resistance markers in Pfcrt and Pfmdr1 genes in isolates from Honduras and Nicaragua, 2018–2021. Malar J. 2021;20:465.
Article
CAS
PubMed
PubMed Central
Google Scholar
Veiga MI, Ferreira PE, Gil PJ. Multiplex PCR-RFLP methods for Pfcrt, Pfmdr1 and Pfdhfr mutations in Plasmodium falciparum. Mol Cell Probes. 2006;20:100–4.
Article
CAS
PubMed
Google Scholar
Mula P, Fernandez-martinez A, Lucio A, De, Ramos JM, Reyes F, Gonzalez V, et al. Detection of high levels of mutations involved in anti-malarial drug resistance in Plasmodium falciparum and Plasmodium vivax at a rural hospital in Southern Ethiopia. Malar J. 2011;10:214.
Article
CAS
PubMed
PubMed Central
Google Scholar
Jaleta F, Garoma G, Gerenfes T. Evaluation of malaria microscopy diagnosis performance in public hospitals of Eastern and Central part of Oromia Region, Ethiopia, 2019. Pathol Lab Med Int. 2020;12:1–8.
Article
Google Scholar
Schunk M, Kumma WP, Miranda IB, Osman ME, Roewer S, Alano A, et al. High prevalence of drug-resistance mutations in Plasmodium falciparum and Plasmodium vivax in Southern Ethiopia. Malar J. 2006;5:54.
Article
PubMed
PubMed Central
Google Scholar
MOH. Ethiopia Malaria Elimination Strategic Plan: 2021–2025. Ministry of Health; Addis Ababa, Ethiopia; 2020.
Yobi DM, Kayiba NK, Mvumbi DM, Boreux R, Kabututu PZ, Situakibanza HNT, et al. Molecular surveillance of anti-malarial drug resistance in Democratic Republic of Congo: high variability of chloroquine resistance and lack of amodiaquinoresistance. Malar J. 2020;19:121.
Article
CAS
PubMed
PubMed Central
Google Scholar
Al-Mekhlafi AM, Mahdy MAK, Al-Mekhlafi HM, Azazy AA, Fong MY. High frequency of Plasmodium falciparum chloroquine resistance marker (Pfcrt T76 mutation) in Yemen: an urgent need to re-examine malaria drug policy. Parasit Vectors. 2011;4:94.
Article
CAS
PubMed
PubMed Central
Google Scholar
Adam R, Mukhtar MM, Abubakar UF, Damudi HA, Muhammad A, Ibrahim SS. Polymorphism analysis of Pfmdr1 and Pfcrt from Plasmodium falciparum isolates in Northwestern Nigeria revealed the major markers associated with antimalarial resistance. Diseases. 2021;9:6.
Article
CAS
PubMed
PubMed Central
Google Scholar
Das S, Tripathy S, Chattopadhayay S, Das B, Kar Mahapatra S, Hati AK, et al. Progressive increase in point mutations associates chloroquine resistance: even after withdrawal of chloroquine use in India. IJP Drugs Drug Resist. 2017;7:251–61.
Article
Google Scholar
Johora FT, Elahi R, Nima MK, Hossain MS, Rashid H, Kibria MG, et al. Persistence of markers of chloroquine resistance in Plasmodium falciparum in Bangladesh. Am J Trop Med Hyg. 2021;104:276–82.
Article
CAS
PubMed
Google Scholar
Ebel ER, Reis F, Petrov DA, Beleza S. Historical trends and new surveillance of Plasmodium falciparum drug resistance markers in Angola. Malar J. 2021;20:175.
Article
CAS
PubMed
PubMed Central
Google Scholar
Bwire GM, Ngasala B, Mikomangwa WP, Kilonzi M, Kamuhabwa AAR. Detection of mutations associated with artemisinin resistance at K13- propeller gene and a near complete return of chloroquine susceptible falciparum malaria in Southeast of Tanzania. Sci Rep. 2020;10:3500.
Article
CAS
PubMed
PubMed Central
Google Scholar
Chebore W, Zhou Z, Westercamp N, Otieno K, Shi YP, Sergent SB, et al. Assessment of molecular markers of anti-malarial drug resistance among children participating in a therapeutic efficacy study in Western Kenya. Malar J. 2020;19:291.
Article
CAS
PubMed
PubMed Central
Google Scholar
Balikagala B, Sakurai-Yatsushiro M, Tachibana SI, Ikeda M, Yamauchi M, Katuro OT, et al. Recovery and stable persistence of chloroquine sensitivity in Plasmodium falciparum parasites after its discontinued use in Northern Uganda. Malar J. 2020;19:76.
Article
CAS
PubMed
PubMed Central
Google Scholar
Afoakwah R, Boampong JN, Egyir-Yawson A, Nwaefuna EK, Verner ON, Asare KK. High prevalence of PfCRT K76T mutation in Plasmodium falciparum isolates in Ghana. Acta Trop. 2014;136:32–6.
Article
CAS
PubMed
Google Scholar
Edogun HA, Daramola GO, Ojerinde AO, Esan CO, Adegbuyi AT. Prevalence of mutant alleles responsible for chloroquine resistance among Plasmodium falciparum isolates in North Central, Nigeria. J Adv Biol Biotechnol. 2019;20:1–8.
Article
Google Scholar
Lo E, Hemming-schroeder E, Yewhalaw D, Nguyen J, Kebede E, Zemene E, et al. Transmission dynamics of co-endemic Plasmodium vivax and Plasmodium falciparum in Ethiopia and prevalence of anti-malarial resistant genotypes. PLoS Negl Trop Dis. 2017;11:e0005806.
Article
PubMed
PubMed Central
Google Scholar
Otienoburu SD, Maiga-Ascofare O, Schramm B, Jullien V, Jones JJ, Zolia YM, et al. Selection of Plasmodium falciparum Pfcrt and Pfmdr1 polymorphisms after treatment with artesunate–amodiaquine fixed dose combination or artemether–lumefantrine in Liberia. Malar J. 2016;15:452.
Article
PubMed
PubMed Central
Google Scholar
Asare KK, Africa J, Mbata J, Opoku YK. The emergence of chloroquine-sensitive Plasmodium falciparum is influenced by selected communities in some parts of the Central Region of Ghana. Malar J. 2021;20:447.
Article
CAS
PubMed
PubMed Central
Google Scholar
Chidimatembue A, Svigel SS, Mayor A, Aide P, Nhama A, Nhamussua L, et al. Molecular surveillance for polymorphisms associated with artemisinin-based combination therapy resistance in Plasmodium falciparum isolates collected in Mozambique, 2018. Malar J. 2021;20:398.
Article
CAS
PubMed
PubMed Central
Google Scholar
Malmberg M, Ferreira PE, Tarning J, Ursing J, Ngasala B, Bjorkman A, et al. Plasmodium falciparum drug resistance phenotype as assessed by patient antimalarial drug levels and its association with Pfmdr1 polymorphisms. J Infect Dis. 2013;207:842–7.
Article
CAS
PubMed
Google Scholar
Venkatesan M, Gadalla NB, Stepniewska K, Dahal P, Nsanzabana C, Price RN, et al. Polymorphisms in Plasmodium falciparum chloroquine resistance transporter and multidrug resistance 1 genes: parasite risk factors that affect treatment outcomes for Plasmodium falciparum malaria after artemether–lumefantrine and artesunate–amodiaquine. Am J Trop Med Hyg. 2014;91:833–43.
Article
PubMed
PubMed Central
Google Scholar
Dimbu PR, Horth R, Candido LM, Ferreira M, Caquece F. Continued low efficacy of artemether–lumefantrine in Angola in 2019. Antimicrob Agents Chemother. 2021;65:e01949-20.
Article
PubMed
PubMed Central
Google Scholar
Adamu A, Jada MS, Haruna HMS, Yakubu BO, Ibrahim MA, Balogun EO, et al. Plasmodium falciparum multidrug resistance gene-1 polymorphisms in Northern Nigeria: implications for the continued use of artemether–lumefantrine in the region. Malar J. 2020;19:439.
Article
CAS
PubMed
PubMed Central
Google Scholar