- Open Access
Sexual recombination is a signature of a persisting malaria epidemic in Peru
© Sutton et al; licensee BioMed Central Ltd. 2011
- Received: 30 June 2011
- Accepted: 31 October 2011
- Published: 31 October 2011
The aim of this study was to consider the impact that multi-clone, complex infections have on a parasite population structure in a low transmission setting. In general, complexity of infection (minimum number of clones within an infection) and the overall population level diversity is expected to be minimal in low transmission settings. Additionally, the parasite population structure is predicted to be clonal, rather than sexual due to infrequent parasite inoculation and lack of recombination between genetically distinct clones. However, in this low transmission of the Peruvian Amazon, complex infections are becoming more frequent, in spite of decreasing infection prevalence. In this study, it was hypothesized that sexual recombination between distinct clonal lineages of Plasmodium falciparum parasites were altering the subpopulation structure and effectively maintaining the population-level diversity.
Fourteen microsatellite markers were chosen to describe the genetic diversity in 313 naturally occurring P. falciparum infections from Peruvian Amazon. The population and subpopulation structure was characterized by measuring: clusteredness, expected heterozygosity (He), allelic richness, private allelic richness, and linkage disequilibrium. Next, microsatellite haplotypes and alleles were correlated with P. falciparum merozoite surface protein 1 Block 2 (Pf msp1-B2) to examine the presence of recombinant microsatellite haplotypes.
The parasite population structure consists of six genetically diverse subpopulations of clones, called "clusters". Clusters 1, 3, 4, and 6 have unique haplotypes that exceed 70% of the total number of clones within each cluster, while Clusters 2 and 5 have a lower proportion of unique haplotypes, but still exceed 46%. By measuring the He, allelic richness, and private allelic richness within each of the six subpopulations, relatively low levels of genetic diversity within each subpopulation (except Cluster 4) are observed. This indicated that the number of alleles, and not the combination of alleles, are limited. Next, the standard index of association (IAS) was measured, which revealed a significant decay in linkage disequilibrium (LD) associated with Cluster 6, which is indicative of independent assortment of alleles. This decay in LD is a signature of this subpopulation approaching linkage equilibrium by undergoing sexual recombination. To trace possible recombination events, the two most frequent microsatellite haplotypes observed over time (defined by either a K1 or Mad20) were selected as the progenitors and then potential recombinants were identified in within the natural population.
Contrary to conventional low transmission models, this study provides evidence of a parasite population structure that is superficially defined by a clonal backbone. Sexual recombination does occur and even arguably is responsible for maintaining the substructure of this population.
- Allelic Richness
- Unique Haplotype
- Sexual Recombination
- Complex Infection
- Active Case Detection
The events that shape a local population structure of Plasmodium falciparum are not fully understood. There is a definite link between the parasite transmission rate and the amount of circulating genetic diversity within a local population; high transmission regions typically have larger pools of genetically diverse parasites, while low transmission regions typically have a limited pool of genetically diverse parasites. However, understanding how this diversity is maintained within a population is fairly unresolved due to the parasite's intricate life cycle, which has maintained both sexual and asexual phases. Unfortunately, the depth of the answer to this question is constrained by the genetic markers and molecular methods used unravel the evolutionary history by surveying the population diversity. Until deep sequencing becomes more feasible, microsatellite markers provide a relatively thorough guide to the local, and perhaps global, evolutionary history of P. falciparum, as well as other eukaryotic parasites [2–12]. Considering how genetic diversity is maintained and/or generated on a local level is a priority for advancing epidemiological applications on a molecular level by understanding how this parasite evolves in natural settings.
The contribution of sexual recombination between two unrelated P. falciparum clones on the population structure remains a topic for debate. Several studies have concluded that P. falciparum parasites are essentially clonal due to infrequent recombination between different clonal lineages, while others have concluded that sexual recombination occurs more frequently and contributes greatly to the overall population structure [13–26]. Unfortunately, measuring the contribution of population-level genetic diversity generated by sexual recombination is not straightforward, particularly in an organism like P. falciparum, which spends a great proportion of its life cycle in a haploid, asexual form. Several studies have investigated the contribution of genetic diversity though sexual recombination by measuring the non-random mating preference within the mosquito gut; however, data from these studies versus studies of recombination between clones from genetically distinct lineages, are incongruous [26–29].
Recombination between genetically distinct clones (or clonets) can only occur if a mosquito takes up more than one during a single blood meal or accumulates multiple clones during several interrupted feedings. The probability of a mosquito carrying multiple clones is then a function of the longevity and biting rate of the mosquito, mosquito uptake of gametocytes from different parasites clones (or clonets), and the amount of genetic diversity circulating within a parasite population [30–34]. In high transmission regions, the contribution of sexual recombination is likely to be higher and more apparent than in low transmission regions, due to increased opportunities for mosquitoes to accumulate different clones (or clonets) within the same blood meal [35–41]. Furthermore, regions of high transmission tend to have higher levels of genetic diversity with a notable decay in the linkage disequilibrium between loci, while in low transmission regions, the genetic diversity is markedly lower and disequilibrium between genetic markers is high . However, even if sexual recombination between parasites of different lineages occurs at a low frequency, as is proposed in low transmission regions, the survival of the recombinants might impact the population structure and epidemiology of malaria. To test this, a longitudinal approach is necessary.
Since 2003, the Malaria Immunology and Genetics in the Amazon (MIGIA) project has been longitudinally following a low transmission (< 0.5 P. falciparum infections/person/year) cohort of approximately 2,000 participants located south of Iquitos, Peru in the community of Zungarococha. As this project progressed, snap-shot glances of the population-level diversity were made by using single copy, antigenic P. falciparum merozoite surface proteins (Pf msp)-1, -3, and -6. Characterization with these markers showed that the overall population-level diversity was comparable to that of other low transmission regions [41, 43–45]. However, now with more than five years of longitudinal sampling data, it is clear that the population structure is far more complex than expected. A recent study, using 14 microsatellite markers scattered across 10 chromosomes, found 182 genetically distinct microsatellite haplotypes from 302 P. falciparum infections occurring between 2003 and 2007 . Extensive population genetic analysis showed that the immigration of new clones into this low transmission population was highly improbable and could not explain the population-level diversity . The investigators concluded that the level of parasite genomic diversity was effectively maintained on a temporal (2003-2007) and spatial (four networked villages) level by the loss of once frequent clones and the introduction of newly evolved ones .
The objective of this study was to understand how this level of diversity has been maintained temporally within this low transmission population. Across five years of longitudinal sampling, there has been a significant increase in the proportion of complex infections to single-clone infections, despite decreasing P. falciparum infection prevalence over time. This increased proportion of complex infections is curious, but not unique; a similar finding was observed in Thailand . Such a phenomenon has the potential to set up an ecological scenario, whereby the constituents of complex infections have been provided with an increased opportunity to recombine over time.
Consequently, it was hypothesized that sexual recombination between distinct clonal lineages of Plasmodium falciparum parasites were altering the subpopulation structure and effectively maintaining the population-level diversity in this local population. Sexual recombination between distinct clones (clonets) was traced in this population using 14 microsatellite markers scattered across 10 chromosomes and then correlated with an independent antigenic marker, Pf msp1 Block 2 (Pf msp1-B2). The main allelic families of Pf msp1-B2 were found to correlate significantly with specific alleles from each of the microsatellite loci and were also found to have distinct microsatellite haplotype signatures. On a population-level, this resulted in the observation of a rigid clonal backbone that could be defined by Pf msp1-B2 main allelic families. However, this clonal population structure breaks down due to increased sexual recombination between constituents of complex infections that have been propagated together over time. The consequences of such sexual recombination could impact parasite evolution of drug resistance, host's susceptibility to infection, and even the expansion of new clonal variants.
DNA samples were selected from the Malaria Immunology and Genetics in the Amazon longitudinal cohort study in the community of Zungarococha, located in the San Juan District south of Iquitos, Peru. Details on the study site and design are described in . In brief, this longitudinal study employs both active and passive case detection methods. Of the community of approximately 2,100 individuals, 1,945 individuals participated each year. In active case detection (ACD) there are at least six household visits during the seven month malaria season: one at the beginning of the malaria season, followed by four consecutive weekly visits during malaria season, and then one at the end of the malaria season.
Infections were defined as one or more malaria-positive sample point(s) during the one-month of follow-up (termed "infection-month). The sampling could, therefore, begin before the initial malaria positive sample or after, with an overall sampling time frame of less than or equal to 30 days (4 weeks of sampling) [41, 46, 47]. Infections were defined as complex when more than one allele was present within a single sample or over the infection-month as determined by Pf msp1-B2 genotyping. All blood samples, positive or negative by microscopy, underwent DNA extraction by Qiagen DNeasy Blood and Tissue Kits and were tested for presence of Plasmodium species using a nested multiplex PCR method targeting DNA encoding the small subunit ribosomal protein (ssrDNA) (Qiagen Inc., Valencia, CA) .
All passive case detection (PCD) samples collected from participating individuals were included in this study. Members of our field team, along with Ministerio de Salud (MINSA) personnel, staff the local health post, which services the community of Zungarococha. All individuals presenting with a fever are screened for malaria infection and treated if an infection is detected. These services are provided at no cost to the patient.
All protocols were reviewed and approved by the Institutional Review Boards at the University of Alabama at Birmingham (former institution), New York University School of Medicine, and MINSA. Written, informed consent/assent was obtained from all participants in this study.
Microsatellite marker selection and amplification
Fourteen microsatellite markers were chosen from the microsatellite linkage map for amplification. Descriptive haplotypes were assembled for clones by concatenating the alleles detected at each microsatellite locus. Infections were classified as complex when two or more loci contained multiple alleles. Haplotype phasing was confirmed by cloning by limiting dilution (see below). All other methods are described in Branch et al. . Summary information on microsatellite marker loci, number of alleles, and the amount of diversity per locus can be found in Additional file 1.
Cloning by limiting dilution
Venous blood from P. falciparum positive individuals was collected in a BD Vacutainer® (Becton-Dickinson (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) and parasites were harvested and cryopreserved in a culture bank. A selection of 15 samples, determined to have two different alleles (30 different clones) based on Pf msp1-B2 genotyping, were cultured in vitro. At 3% parasitaemia, small-volume aliquots were passed to new culture flasks with fresh culture media to separate and expand the clones. At each passing, including day 0, a small aliquot of the culture was taken for DNA extraction and amplification by PCR for Pf msp1-B2 genotyping. Genotyping was performed at each passage until infections were passed as single clones over three passages. Additionally, microsatellite genotyping (using 14 microsatellites) was performed in triplicate on all samples before and after separating the clones by limiting dilution.
Pf msp1-B2 genotyping analysis
PCR genotyping of Plasmodium falciparum merozoite surface protein 1 block 2 (Pf msp1-B2) was attempted on all available samples that were microscopy and/or PCR positive for P. falciparum between 2003 and 2007 (858 P. falciparum infection-months) [40, 41]. Of the 858 infection-months, 814 were reproducibly genotyped. Previously reported genotyping methods were used for main allelic family analysis, using all permutations of primers to detect any allele families that were one allele type in the 5' direction and a different allele type in the 3' direction [40, 41, 44, 49]. Of the 194 complex infections, as defined by Pf msp1-B2, there were only 46 (23.7%) instances when an infection was found to have more than one clone due to the detection of a different allele over time (≤ 30 days). These 46 infections were distributed over the five years of this study and did not bias the detection of complex infections.
Genetic diversity data analysis
Structure 2.2, a Bayesian programme for grouping genetically related isolates, was used to define the population structure . Methods for this analysis were previously reported in Branch et al. . In brief, individual microsatellite haplotype profiles were assessed by the clustering algorithm and each haplotype was then given an estimated membership coefficient (Q) that translates to the fraction of relatedness (or ancestry) within/between each cluster . Q is used to establish the clusters (K), which are reported as an admixture proportion. Clusteredness was used to determine the most accurate number of clusters representative of this population. Clusteredness calculations measure the average relatedness of the individual membership coefficients (Q) and estimate the extent to which individual infections belong to a single cluster, rather than to a combination of clusters . For each value of K (pre-specified to equal between 1 and 10), the clustering algorithm was performed 5 times for 10,000 Monte Carlo Markov Chain iterations, followed by a burn-in period of 10,000 iterations. These results were then output and averaged to assess the clusteredness calculation for each value of K. Population differentiation was confirmed using Fst and expected heterozygosity (He) (Arlequin v184.108.40.206) . No population structure was observed for any K-value above K = 10.
Minimum spanning network
A minimum spanning network (MSN) was developed in Network 220.127.116.11 using the microsatellite haplotype profiles . For the MSN, the sample identifications were coded with a combination of the cluster-population (Clusters 1-6) and infection-type (complex or single-clone) information associated with each sample, allowing us to (1) visualize the relatedness between parasite infections, (2) examine how closely related the clusters were to one another, and (3) determine the frequency of complex versus single-clone infections in each cluster subpopulation.
The standard index of association (IAS) was used to test for statistical independence of alleles at each of the 14 microsatellite loci for all subpopulations (LIAN v3.0) . IAS uses a robust Monte Carlo simulation that accounts for differences between the observed (V D ) and expected (V e ) mismatch variances of alleles, provides a measurement for linkage and tests for significance. If V D and V e are significantly different, then independent assortment of alleles cannot explain the observed diversity and the alleles are in disequilibrium.
Allelic and private allelic richness
Subpopulation diversity was calculated by measuring the allelic richness (mean number of alleles per locus) and private allelic richness (number of alleles private to a specific population) using a rarefaction approach for both measurements to model the data based on subpopulation sample-size (ADZE v1.0) .
Unless otherwise specified, all data analyses were performed using JMP® v8.0.2 software (SAS Institute Inc., Cary, NC).
Ecology of complex infections
Pf msp1-B2 genotyping results for all samples collected between 2003 and 2007
Samples selection breakdown
Total number of clones:
Total number of clones:
Pf msp1-B2 analysis
This increased detection of complex infections over time is not due to sampling bias. The average number of complex infections detected through passive case detection (PCD) is significantly lower than those detected though active case detection (ACD) for each year of this study between 2004 and 2007 (p < 0.001, χ2). Also, the distribution of complex infections over time within each of the four networked-villages was proportionate; there was no indication that one or more of the villages may be defined by having a greater transmission of complex infections.
Alleles determined by each of the 14 microsatellite loci were assembled into a single haplotype for each clone within an isolate. To confirm the accuracy of microsatellite haplotype phasing, 30 parasite clones were isolated from complex infections by in vitro culture, using cloning by limiting dilution methods (See methods). Microsatellite genotyping was performed before and after isolating the 30 clones. Twenty-three of the 30 clones were identical to the microsatellite haplotypes compiled without cloning out by limiting dilution. Of the seven discrepant microsatellite haplotypes, five had discrepancies in one locus (92.9% match with non-isolated haplotype) and two had discrepancies in two loci (85.7% match with non-isolated haplotype). However, the correct locus was always observed in at least one or two of the genotyping replicates, but never all three as was observed in the 23 clones.
In total, there were 313 clones detected in the 243 infection-months selected for microsatellite analysis; 62 of these infection-months were determined to be complex infections. These results were directly correlated to the Pf msp1-B2 genotypic classification of infection-type and a kappa test was used to assess the strength of agreement between the proportions of complex versus single-clone infections defined by both genotyping methods.
The average COI (total number of clones divided by the total number of infection-months) differed slightly between the Pf msp1-B2 (305 clones detected in 243 infections, COI = 1.26) and microsatellite genotyping (313 clones detected in 243 infections, COI = 1.29) methods (Table 2). Of the 243 infection-months selected, 54 of these were defined as complex by Pf msp1-B2 genotyping (43 interfamily and 11 intrafamily), while 62 were defined as complex by microsatellite genotyping (14 markers scattered over 10 different chromosomes) (Table 2). Detecting an increased number of complex infections using the microsatellite assay was anticipated due to the use of multiple loci. The kappa value was equal to 0.523 (95% CI = 0.447-0.598), indicating moderate strength of agreement between these genotyping methods.
Microsatellite haplotype population structure analyses
Structure v2.2  was used to describe the subpopulation structure based on the 14 microsatellite loci haplotypes for all clones (N = 313) from all 243 infections in this sample-set. A post-hoc clusteredness calculation was performed to determine accuracy of predetermined number of clusters (K) . The highest clusteredness value over 10 simulations showed K equal to 6, with a clusteredness value equal to 0.81. Arlequin v18.104.22.168  was used to test the population differentiation (Fst) between these clusters. Each of the six clusters was found to represent independent subpopulations (p < 0.01, Pairwise Fst). On a spatial and temporal level, these six cluster subpopulations were maintained in all four-networked villages across each year of this study.
Recombination as a factory for diversity
Underrepresented identical haplotypes
Measurements and indices of subpopulation genetic diversity
No. of samples
No. of haplotypes
Private allelic richness
Interestingly, in spite of the high frequency of unique haplotypes within each cluster, there were relatively low levels of genetic diversity within each of these subpopulations. He (Arlequin v22.214.171.124) was used to measure the genetic diversity based on the relative frequencies of the different alleles within each of the subpopulations. There was low He in Clusters 1, 2, 3, 5, and 6, with a range of 0.15 to 0.40. To further investigate the subpopulation diversity, allelic richness (mean number of alleles per locus) and private allelic richness (number of alleles private to a specific population) were measured using a rarefaction approach to standardize and model the data based on subpopulation sample-size (ADZE v1.0). After correction for sample-size, Clusters 1, 2, 3, 5, and 6 averaged 4 alleles per locus, with approximately 23% of the alleles detected within each of these five subpopulations identified as private alleles. Cluster 4 deviates from the trend observed in Clusters 1, 2, 3, 5, and 6, with a moderately higher He equal to 0.593 and an allelic richness equal to 4.71 alleles per locus with approximately 80% of the alleles detected identified as private alleles (Table 3).
The standard index of association (IAS) for each cluster was measured to test for statistical independence amongst alleles (independent assortment of alleles) at each of the 14 microsatellite loci (Table 3). Based on this method, Clusters 1, 2, 3, 4, and 5 were in linkage disequilibrium (LD) (p < 0.005, 10000-step Markov Chain analysis, one-tailed t-test, LIAN v3.0), while break down in LD was observed in Cluster 6 (p = 0.072, 10000-step Markov Chain analysis, one-tailed t-test, LIAN v3.0) (Table 3). This decay in LD associated with Cluster 6 is indicative of an independent assortment of alleles, which is a signature of this subpopulation approaching linkage equilibrium by undergoing sexual recombination.
Breakdown in correlation between independent sets of genetic markers in the complex infection subpopulation
Next, Pf msp1-B2 genotypes were correlated to the six cluster subpopulations as determined by using the 14 microsatellite loci. Using a probability model, the expected Pf msp1-B2 genotype frequencies were compared to the observed genotype frequencies in all six clusters. With the exception of Cluster 6, one Pf msp1-B2 main allelic family (K1 or Mad20), and often one specific Pf msp1-B2 allele (repeat length), significantly correlated with the clones defining each cluster (p < 0.001, χ2). Clusters 1 and 5 were significantly comprised of single-clone Mad20 alleles, while Clusters 2 and 4 were significantly comprised of single-clone K1 alleles. Cluster 6, the exception to this population structure, was significantly comprised interfamily complex infections (K1 plus Mad20), and also had the highest number of interfamily complex infections of all six subpopulations (p = 0.0044, Fisher's exact). The subpopulation structure of Cluster 6 may explain the previously observed decay in LD associated with this cluster, as this cluster may have evolved from recombination events between the two most frequently observed genotypes in this population.
Appearance of recombined haplotypes over time
However, this correlation also enabled the meticulous tracing of sexual recombination across the 14 microsatellite loci. To trace possible recombination events, the two most frequent microsatellite haplotypes observed over time (defined by either a K1 or Mad20) were selected as the progenitors and then potential recombinants were identified in within the natural population. Six examples of potential recombinants that may have arisen from recombination between the Mad20- and K1-like dominant haplotypes are illustrated in Figure 5B. There is clear evidence for a breakdown in the correlation between these two independent genetic markers, likely due to recombination between clones alternatively identified by these two main allelic families.
Finally, this genome homogenization was examined over time. Populations were divided into "early years" (2003-2005) and "late years" (2006-2007). For complex infections, most of which belonged to Cluster 6, there was a 36.1% (13 new loci pairs and 36 repeated loci pairs) increase in the number of new loci combinations detected over time. The frequency of these new allelic combinations became rapidly integrated into the natural population, defining 34.3% of the complex infections detected between 2006 and 2007. In contrast, there was only a 16.0% (8 new loci pairs and 50 repeated loci pairs) increase in the number of new loci combinations observed in single-clone infections. These new combinations only defined 6.7% of the single-clone infection population detected between 2006 and 2007. The frequency of new loci combinations emerging in this study over time was significantly higher in complex than single-clone infections (p < 0.0001, χ2).
The increased combination of loci pairs and their increased frequency over time is consistent with recombination between constituents of complex infections, a distinct evolutionary signature from that of the single-clone infections. The detection of new alleles over time cannot explain the rise of new combinations of alleles, as there were only three new alleles detected at an exceedingly low frequency in the population between 2006 and 2007. It is clear that these new combinations have arisen from recombination and not by the introduction of new alleles.
In this study, the evolutionary impact of multiple clone, complex infections within a local and low transmission population in the Peruvian Amazon between 2003 and 2007 was investigated. Previously, researchers have found that in exceedingly low transmission regions, such as the Karen Refugee Camp in Thailand, there were more complex infections than expected by chance . Though unexpected in low transmission settings, a niche with a high frequency of complex infections increases the potential for sexual recombination between genetically distinct clones within local populations. The formation, propagation, and ultimately the survival of complex infections in low transmission regions are of particular interest because these types of infections provide an increased opportunity for genetic diversity to be maintained through recombination. A study of this nature is not feasible in high transmission regions because the complexity of infection is generally high due to overlapping infections and virtually impossible to separate infections into individual clonal constituents.
An earlier study, which examined the ecology of complex infections in this cohort from 2003-2004, found that complex infections within humans were not formed by an accumulation of clones by superinfection, but rather, complex infections were being propagated as a single unit during a blood meal . In this study, Pf msp1-B2 was genotyped from all P. falciparum positive samples collected between 2003 and 2007 to determine the frequency of complex infections over time. This finding was supported by the sampling and genotyping strategies used in this cohort study. All samples collected, regardless of a positive or negative diagnosis by microscopy undergo species-specific PCR. Due to the fact that many of infections in this community are asymptomatic, it is particularly important to consider the possibility of subpatent carriers. For this reason, sampling occurs over a one-month time-period and final genotypes for all infections are concatenated from multiple sample collections (4-6 collections over 4-weeks), rather than a single time point; this time-point genotyping is referred to as infection-months in this manuscript.
When the longitudinal genotyping data was correlated to infection prevalence data between years 2003 and 2007, the frequency of complex infections were found to increase significantly from 16.3% in 2004 to 35.6% in 2007 in spite of a substantial decrease in the infection prevalence from 0.31 infections/person/month in 2004 to 0.12 infections/person/month in 2007, while the (p < 0.001, χ2). This dynamic does not support an environment whereby complex infections were being generated by superinfection, but rather it provides additional evidence that the complex infections in this low transmission region were being propagated as single evolutionary-units.
The increase in complex infections relative to the single-clone infections was striking given the overall decrease in infection prevalence over the years of this study (Figure 1). Two different scenarios can be speculated to explain this increase over time that is not related to the human host immune response. One possible explanation is that the increase in complex infections over time is an ecological phenomenon, rather than differential survival probabilities of complex versus single-clone infections. For example, it is possible that there is an ecological niche that maintains the transmission of complex infections over time, like mosquito breeding sites or potential reservoirs. However, the distribution of complex infections over time within each of the four networked-villages was proportionate, with no indication that one or more of the villages have a greater frequency of complex infections. Another possible explanation is that complex infections are more readily transmitted from the human host to the mosquito host [56–68]. This may be linked with an environmental stimulus that signals an increased production of gametocytes when parasites are under stress [59–63], but it is still unclear whether or not this may also be linked to disease outcome within the human host.
Regardless of why complex infections were increasing in the population, the result of such infections being propagated longitudinally would be a reduced chance of encountering the same genotype over successive infections due to increased opportunities for recombination . The hypothesis in this study was that despite a superficial clonal population structure, sexual recombination between distinct clonal lineages of P. falciparum parasites is altering the sub-structure of this population. To directly test this hypothesis, previously defined criteria for distinguishing between clonal and sexual populations was used .
Using microsatellites to longitudinally characterize the population and within-host level genetic diversity, it is clear that the parasite population can be divided into six distinct cluster subpopulations that were maintained over time in this study. The frequency of identical and unique microsatellite haplotypes were then investigated within the total population and within each of the six cluster subpopulations. Of the 313 clones in the total population, there were 197 unique haplotypes observed (Table 3). These unique haplotypes were not isolated to a particular subpopulation, but rather were found across each of the six subpopulations (clusters) of related clones. Clusters 1, 3, 4, and 6 have unique haplotypes that exceed 70% of the total number of clones within each cluster, while Clusters 2 and 5 have a lower proportion of unique haplotypes, but still exceed 46% (Table 3). This contradicts what is expected in the null hypothesis.
The excess of unique haplotypes was further investigated by measuring the He, allelic richness, and private allelic richness within each of the six cluster subpopulations (Table 3). These analyses revealed that the number of alleles is limited, while the combination of alleles (which comprise the haplotypes), is not limited. This indicated that a breakdown in linkage between the loci could help to explain the excess of unique haplotypes. Next, a test for independent assortment of alleles was performed using the standard index of association (IAS). This test indicated a significant decay in LD restricted to Cluster 6 (Table 3). On a parasite level, predicting the genetic exchange during one round of sexual recombination is at best an educated guess, but over ten years of persisting recombination, it is virtually impossible to do, even with sophisticated models. Due to the significant decay in LD associated with Cluster 6, this cluster became a model for recombination in this study; however, Cluster 6 was not the only cluster where recombinants were identified.
To further consider the breakdown of LD in Cluster 6, the correlation between the microsatellite haplotypes and the single-copy antigenic locus Pf msp1-B2 was examined. Using Pf msp1-B2 as a generic and independent genetic marker permitted a broad characterization of the genome and further insight into the ecology of the infections analyzed. Five of these subpopulations (Cluster 1-5) were significantly comprised of single-clone infections that significantly correlated with specific Pf msp1-B2 main allelic family genotypes (either K1 or Mad20), while Cluster 6 was significantly comprised of complex infections that were a mixture of Pf msp1-B2 main allelic family genotypes (K1 and Mad20). A breakdown in linkage could be readily observed by considering the linkage in the single-clone (K1 or Mad20) versus complex clone infections (K1 and Mad20) (Figure 5; Figure 6). The population structure of the single-clone infections define a clonal backbone with strong linkage to the antigenic individual Pf msp1-B2 genotypes, while the complex infections undergo apparent recombination, which breaks down the linkage between alleles in this backbone. The result of this is an increased number of unique haplotypes over time (Figure 4). Further, it was possible to single-out potential progenitors of recombined clones, which strengthens the evidence of sexual recombination contributing to the parasite genetic diversity within this population (Figure 5).
Repeated or recursive recombination naturally results in the homogenization of different parasite clones or strains, unless there is some barrier to recombination that maintains a clonal backbone structure. Although microsatellite markers are considered neutral, there might be linkage with antigens scattered across the genomes. If this is the case, the recombination may breakdown linkage between antigen encoding genes, which is particularly useful if the human host is immune to reinfection by recently encountered genotypes [34, 64–72]. Others have suggested that recombination is favorable in changing environments, like parasite-host infections that are often modulated by the immune response [34, 72]. However, the observation of recombined microsatellite haplotypes in this study could also be due to one particular locus under selection. That is, it is possible that a particular mutation/allele or combination of alleles, which may have been fixed in the population during the propagation of complex infections over time, resulting in a population with a slight selective advantage [65–68, 73].
In the most practical context, this study shows that genetic diversity can increase even when there is low transmission. This scenario is likely to be reencountered in the future as the current P. falciparum elimination efforts are effectively reducing clinical malaria in many countries across the world. The expansion of recombined clones within a local population could increase host susceptibility to infection, and also provide more opportunities for the evolution of drug resistance.
The aim of this study was to consider the impact that multi-clone, complex infections have on a parasite population structure in a low transmission setting. Contrary to conventional low transmission models, this study provides evidence of a parasite population structure that is superficially defined by a clonal backbone. Sexual recombination does occur and even arguably is responsible for maintaining the substructure of this population. The maintenance of genetic diversity in this population is likely a consequence of the long-term propagation of complex infections in this region. The fact that these recombinants were surviving in this population, despite a decreasing prevalence rate, raises an important question on the fitness of these parasites and/or the accumulation of clones within one infection.
The authors declare that they have no competing interests.
This study was supported by R01 grant AI064831 from the National Institute of Health/National Institute of Allergy and Infectious Disease (year 2005--present). This work began as part of a fellowship award from the Howard Hughes Medical Institute fellowship for developing and transferring new technologies to our ongoing study in Peru. We especially thank Ms. Gisely Hijar from the Laboratorio de Biotecnologia y Biologia Molecular, Instituto Nacional de Salud, Lima, Peru and Dr. Carmen R. Barnes for performing the initial genotyping experiments. Dr. Patrick L. Sutton was supported by the Ruth L. Kirschstein National Research Service Award postdoctoral fellowship, Training Program in Infectious Diseases and Basic Microbiological Mechanisms at New York University School of Medicine, Division of Medical Parasitology (5T32AI007180-28, NIH/NIAID).
We would like to thank the Zungarococha community members and authorities for their ongoing commitment to the Malaria Immunology and Genetics in the Amazon (MIGIA) Study. We thank Dr. Jean Hernandez for overall coordination and Dr. Crystyan Siles for patient care. The field, laboratory, and data researchers and staff were essential for this investigation and continuation of the MIGIA study. We thank Mr. Freddy Alava for enrollment follow-up, Mr. Ever Alvarez and Ms. Anibal Sanchez for microscopy, Mr. Jey Montenegro and Mr. Aldo Montenegro, Ms. Zoila Reategui and Ms. Elva Sanchez for sample collection during patient visits. We thank Ms. Claudia Silva for assistance with culturing malaria isolates, Ms. Sory Vasquez and Mr. Nolberto Tangoa for laboratory sample processing, and Mr. Odilo Alava, Ms. Dania Vela, and Ms. Noelia Escobar for data entry.
Finally, we would like to thank Ms. Melissa Conrad for helpful discussions and suggestions on this manuscript.
- Anderson TJC, Haubold B, Williams JT, Estrada-Franco JG, Richardson L, Mollinedo R, Bockarie M, Mokili J, Mharakurwa S, French N, Whitworth J, Velez ID, Brockman AH, Nosten F, Ferreira MU, Day KP: Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum. Mol Biol Evol. 2000, 17: 1467-1482.View ArticlePubMedGoogle Scholar
- Barker GC: Microsatellite DNA: a tool for population genetic analysis. Trans R Soc Trop Med Hyg. 2002, 96: S21-S24.View ArticlePubMedGoogle Scholar
- Su X-Z, Wellems TE: Toward a high-resolution Plasmodium falciparum linkage map: polymorphic markers from hundreds of simple sequence repeats. Genomics. 1996, 33: 430-444. 10.1006/geno.1996.0218.View ArticlePubMedGoogle Scholar
- Havryliuk T, Ferreira MU: A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences. Mem Inst Oswaldo Cruz. 2009, 104: 67-73.View ArticlePubMedGoogle Scholar
- Imwong M, Sudimack D, Pukrittayakamee S, Osorio L, Carlton JM, Day NP, White NJ, Anderson TJ: Microsatellite variation, repeat array length, and population history of Plasmodium vivax. Mol Biol Evol. 2006, 23: 1016-1018. 10.1093/molbev/msj116.View ArticlePubMedGoogle Scholar
- Cooper A, Tait A, Sweeney L, Tweedie A, Morrison L, Turner CM, MacLeod A: Genetic analysis of the human infective trypanosome Trypanosoma brucei gambiense: chromosomal segregation, crossing over, and the construction of a genetic map. Genome Biol. 2008, 9: R103-10.1186/gb-2008-9-6-r103.PubMed CentralView ArticlePubMedGoogle Scholar
- Ajzenberg D, Bañuls AL, Tibayrenc M, Dardé ML: Microsatellite analysis of Toxoplasma gondii shows considerable polymorphism structured into two main clonal groups. Int J Parasitol. 2002, 32: 27-38. 10.1016/S0020-7519(01)00301-0.View ArticlePubMedGoogle Scholar
- Schwenkenbecher JM, Wirth T, Schnur LF, Jaffe CL, Schallig H, Al-Jawabreh A, Hamarsheh O, Azmi K, Pratlong F, Schönian G: Microsatellite analysis reveals genetic structure of Leishmania tropica. Int J Parasitol. 2006, 36: 237-246. 10.1016/j.ijpara.2005.09.010.View ArticlePubMedGoogle Scholar
- Oliveira RP, Broude NE, Macedo AM, Cantor CR, Smith CL, Pena SD: Probing the genetic population structure of Trypanosoma cruzi with polymorphic microsatellites. Proc Natl Acad Sci USA. 1998, 95: 3776-3780. 10.1073/pnas.95.7.3776.PubMed CentralView ArticlePubMedGoogle Scholar
- Simo G, Njiokou F, Tume C, Lueong S, De Meeûs T, Cuny G, Asonganyi T: Population genetic structure of Central African Trypanosoma brucei gambiense isolates using microsatellite DNA markers. Infect Genet Evol. 2010, 10: 68-76. 10.1016/j.meegid.2009.09.019.View ArticlePubMedGoogle Scholar
- Imwong M, Nair S, Pukrittayakamee S, Sudimack D, Williams JT, Mayxay M, Newton PN, Kim JR, Nandy A, Osorio L, Carlton JM, White NJ, Day NP, Anderson TJ: Contrasting genetic structure in Plasmodium vivax populations from Asia and South America. Int J Parasitol. 2007, 37: 1013-1022. 10.1016/j.ijpara.2007.02.010.View ArticlePubMedGoogle Scholar
- Conrad M, Zubacova Z, Dunn LA, Upcroft J, Sullivan SA, Tachezy J, Carlton JM: Microsatellite polymorphism in the sexually transmitted human pathogen Trichomonas vaginalis indicates a genetically diverse parasite. Mol Biochem Parasitol. 2011, 175: 30-38. 10.1016/j.molbiopara.2010.08.006.PubMed CentralView ArticlePubMedGoogle Scholar
- Rich SM, Hudson RR, Ayala FJ: Plasmodium falciparum antigenic diversity: evidence of clonal population structure. Proc Natl Acad Sci USA. 1997, 94: 13040-13045. 10.1073/pnas.94.24.13040.PubMed CentralView ArticlePubMedGoogle Scholar
- Rich SM, Licht MC, Hudson RR, Ayala FJ: Malaria's eve: evidence of a recent population bottleneck throughout the world populations of Plasmodium falciparum. Proc Natl Acad Sci USA. 1998, 95: 4425-4430. 10.1073/pnas.95.8.4425.PubMed CentralView ArticlePubMedGoogle Scholar
- Coluzzi M: The clay feet of the malaria giant and its African roots: hypotheses and inferences about origin, spread and control of Plasmodium falciparum. Parassitologia. 1999, 41: 277-283.PubMedGoogle Scholar
- Volkman SK, Barry AE, Lyons EJ, Nielsen KM, Thomas SM, Choi M, Thakore SS, Day KP, Wirth DF, Hartl DL: Recent origin of Plasmodium falciparum from a single progenitor. Science. 2001, 293: 482-484. 10.1126/science.1059878.View ArticlePubMedGoogle Scholar
- Tibayrenc M, Kjellberg F, Arnaud J, Oury B, Brenière SF, Dardé ML, Ayala FJ: Are eukaryotic microorganisms clonal or sexual? A population genetics vantage. Proc Natl Acad Sci USA. 1991, 88: 5129-5133. 10.1073/pnas.88.12.5129.PubMed CentralView ArticlePubMedGoogle Scholar
- Babiker HA, Ranford-Cartwright LC, Currie D, Charlwood JD, Billingsley P, Teuscher T, Walliker D: Random mating in a natural population of the malaria parasite Plasmodium falciparum. Parasitology. 1994, 109: 413-421. 10.1017/S0031182000080665.View ArticlePubMedGoogle Scholar
- Su X-Z, Ferdig MT, Huang Y, Huynh CQ, Liu A, You J, Wootton JC, Wellems TE: A genetic map and recombination parameters of the human malaria parasite Plasmodium falciparum. Science. 1999, 286: 1351-1353. 10.1126/science.286.5443.1351.View ArticlePubMedGoogle Scholar
- Conway DJ, Roper C, Oduola AM, Arnot DE, Kremsner PG, Grobusch MP, Curtis CF, Greenwood BM: High recombination rate in natural populations of Plasmodium falciparum. Proc Natl Acad Sci USA. 1999, 96: 4506-4511. 10.1073/pnas.96.8.4506.PubMed CentralView ArticlePubMedGoogle Scholar
- Hey J: Parasite populations: the puzzle of Plasmodium. Curr Biol. 1999, 9: R565-R567. 10.1016/S0960-9822(99)80356-X.View ArticlePubMedGoogle Scholar
- Mu J, Duan J, Makova K, Joy DA, Huynh CQ, Branch OH, Li W-H, Su X-Z: Chromosome-wide SNPs reveal an ancient origin for Plasmodium falciparum. Nature. 2001, 418: 323-326.View ArticleGoogle Scholar
- Hughes AL, Verra F: Very large long-term effective population size in the virulent human malaria parasite Plasmodium falciparum. Proc R Soc London B Biol Sci. 2001, 268: 1855-1860. 10.1098/rspb.2001.1759.View ArticleGoogle Scholar
- Joy DA, Feng X, Mu J, Furuya T, Chotivanich K, Krettli AU, Ho M, Wang A, White NJ, Suh E, Beerly P, Su X-Z: Early origin and recent expansion of Plasmodium falciparum. Science. 2003, 300: 318-321. 10.1126/science.1081449.View ArticlePubMedGoogle Scholar
- Tibayrenc M, Ayala FJ: The clonal theory of parasitic protozoa: 12 years on. Trends Parasitol. 2002, 18: 405-10. 10.1016/S1471-4922(02)02357-7.View ArticlePubMedGoogle Scholar
- Mzilahowa T, McCall PJ, Hastings IM: "Sexual" population structure and genetics of the malaria agent P. falciparum. PLoS One. 2007, 18: 2-Google Scholar
- Razakandrainibe FG, Durand P, Koella JC, De Meeus T, Rousset F, Ayala FJ, Renaud F: "Clonal" population structure of the malaria agent Plasmodium falciparum in high-infection regions. Proc Natl Acad Sci USA. 2005, 102: 17388-17393. 10.1073/pnas.0508871102.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson TJC, Paul REL, Donnelly CA, Day KP: Do malaria parasites mate non-randomly within the mosquito mid gut?. Genet Res. 2000, 75: 285-296. 10.1017/S0016672300004481.View ArticlePubMedGoogle Scholar
- Prugnolle F, De Meeus T: Apparent high recombination rates in clonal parasitic organisms due to inappropriate sampling design. Heredity. 2010, 104: 135-40. 10.1038/hdy.2009.128.View ArticlePubMedGoogle Scholar
- Conway DJ, McBride JS: Population genetics of Plasmodium falciparum within a malaria hyperendemic area. Parasitology. 1991, 103: 7-16. 10.1017/S0031182000059229.View ArticlePubMedGoogle Scholar
- Sinden RE, Hartley RH: Identification of the meiotic division of malarial parasites. J Protozool. 1985, 32: 742-744.View ArticlePubMedGoogle Scholar
- Sinden RE, Hartley RH, Winger L: The development of Plasmodium ookinetes in vitro: an ultrastructural study including a description of meiotic division. Parasitology. 1985, 91 (Pt 2): 227-244.View ArticlePubMedGoogle Scholar
- Walliker D, Quakyi IA, Wellems TE, McCutchan TF, Szarfman A, London WT, Corcoran LM, Burkot TR, Carter R: Genetic analysis of the human malaria parasite Plasmodium falciparum. Science. 1987, 236: 1661-1666. 10.1126/science.3299700.View ArticlePubMedGoogle Scholar
- Hill W, Babiker H: Estimation of numbers of malaria clones in blood samples. Proc R Soc Lond B. 1995, 262: 249-257. 10.1098/rspb.1995.0203.View ArticleGoogle Scholar
- Branch O, Takala S, Kariuki S, Nahlen B, Kolczak M, Hawley W, Lal A: Plasmodium falciparum genotypes, low complexity of infection, and resistance to subsequent malaria in participants in the Asembo Bay Cohort Project. Infect Immun. 2001, 69: 7783-7792. 10.1128/IAI.69.12.7783-7792.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Hay SI, Guerra CA, Gething PW, Patil AP, Tatem AJ, Noor AM, Kabaria CW, Manh BH, Elyazar IR, Brooker S, Smith DL, Moyeed RA, Snow RW: A world malaria map: Plasmodium falciparum endemicity in 2007. PLoS Med. 2009, 6: e1000048-PubMed CentralView ArticlePubMedGoogle Scholar
- Konate L, Zwetyenga J, Rogier C, Bischoff E, Fontenille D, Tall A, Spiegel A, Trape JF, Mercereau-Puijalon O: Variation of Plasmodium falciparum msp1 block 2 and msp2 allele prevalence and of infection complexity in two neighbouring Senegalese villages with different transmission conditions. Trans R Soc Trop Med Hyg. 1999, 93: 21-28.View ArticlePubMedGoogle Scholar
- Paul REL, Day KP: Mating Patterns of Plasmodium falciparum. Parasitol Today. 1998, 14: 197-202. 10.1016/S0169-4758(98)01226-5.View ArticlePubMedGoogle Scholar
- Paul REL, Hackford I, Brockman A, Muller-Graf C, Price R, Luxemburger C, White NJ, Nosten F, Day KP: Transmission intensity and Plasmodium falciparum diversity on the northwestern border of Thailand. Am J Trop Med Hyg. 1998, 58: 195-203.PubMedGoogle Scholar
- Robert F, Ntoumi F, Angel G, Candito D, Rogier C, Fandeur T, Sarthou JL, Mercereau-Puijalon O: Extensive genetic diversity of Plasmodium falciparum isolates collected from patients with severe malaria in Dakar, Senegal. Trans R Soc Trop Med Hyg. 1996, 90: 704-711. 10.1016/S0035-9203(96)90446-0.View ArticlePubMedGoogle Scholar
- Sutton PL, Neyra V, Hernandez JN, Branch OH: Plasmodium falciparum and Plasmodium vivax infections in the Peruvian Amazon: propagation of complex, multiple allele-type infections without superinfection. Am J Trop Med Hyg. 2009, 81: 950-960. 10.4269/ajtmh.2009.09-0132.PubMed CentralView ArticlePubMedGoogle Scholar
- Urdaneta L, Lal A, Barnabe C, Oury B, Goldman I, Ayala FJ, Tibayrenc M: Evidence for clonal propagation in natural isolates of Plasmodium falciparum from Venezuela. Proc Natl Acad Sci USA. 2001, 98: 6725-6729. 10.1073/pnas.111144998.PubMed CentralView ArticlePubMedGoogle Scholar
- Jordan SJ, Branch OH, Castro JC, Oster RA, Rayner JC: Genetic diversity of the malaria vaccine candidate Plasmodium falciparum merozoite surface protein-3 in a hypoendemic transmission environment. Am J Trop Med Hyg. 2009, 80: 479-486.PubMed CentralPubMedGoogle Scholar
- Sutton PL, Clark EH, Silva C, Branch OH: The Plasmodium falciparum merozoite surface protein-1 19 KD antibody response in the Peruvian Amazon predominantly targets the non-allele specific, shared sites of this antigen. Malar J. 2010, 9: 3-10.1186/1475-2875-9-3.PubMed CentralView ArticlePubMedGoogle Scholar
- Neal AT, Jordan SJ, Oliveira AL, Hernandez JN, Branch OH, Rayner JC: Limited variation in vaccine candidate Plasmodium falciparum Merozoite Surface Protein-6 over multiple transmission seasons. Malar J. 2010, 9: 138-10.1186/1475-2875-9-138.PubMed CentralView ArticlePubMedGoogle Scholar
- Branch OH, Sutton PL, Barnes CR, Castro JC, Hussin J, Awadalla P, Guerra GH: Plasmodium falciparum genetic diversity maintained and amplified over five years of a low transmission endemic in the Peruvian Amazon. Mol Biol Evol. 2010, 28: 1973-1986.PubMed CentralView ArticlePubMedGoogle Scholar
- Branch O, Casapia WM, Gamboa DV, Hernandez JN, Alava FF, Roncal N, Alvarez E, Perez EJ, Gotuzzo E: Clustered local transmission and asymptomatic Plasmodium falciparum and Plasmodium vivax malaria infections in a recently emerged, hypoendemic Peruvian Amazon community. Malar J. 2005, 4: 27-10.1186/1475-2875-4-27.PubMed CentralView ArticlePubMedGoogle Scholar
- Rubio JM, Benito A, Roche J, Berzosa PJ, Garcia ML, Mico M, Edu M, Alvar J: Semi-nested, multiplex polymerase chain reaction for detection of human malaria parasites and evidence of Plasmodium vivax infection in Equatorial Guinea. Am J Trop Med Hyg. 1999, 60: 183-187.PubMedGoogle Scholar
- Takala S, Branch O, Escalante AA, Kariuki S, Wootton J, Lal AA: Evidence for intragenic recombination in Plasmodium falciparum: identification of a novel allele family in block 2 of merozoite surface protein-1: Asembo Bay Area Cohort Project XIV. Mol Biochem Parasitol. 2002, 125: 163-171. 10.1016/S0166-6851(02)00237-2.PubMed CentralView ArticlePubMedGoogle Scholar
- Pritchard JK, Stephens M, Donnelly P: Inference of population structure using multilocus genotype data. Genetics. 2000, 155: 945-959.PubMed CentralPubMedGoogle Scholar
- Rosenberg NA, Mahajan S, Ramachandran S, Zhao C, Pritchard JK, Feldman MW: Clines, clusters, and the effect of study design on the inference of human population structure. PLoS Genet. 2005, 1: e70-10.1371/journal.pgen.0010070.PubMed CentralView ArticlePubMedGoogle Scholar
- Excoffier L, Valval G, Schneider S: Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online. 2005, 1: 47-50.PubMed CentralGoogle Scholar
- Polzin T, Daneschmand S: On Steiner trees and minimum spanning trees in hypergraphs. Operations research Letters. 2003, 31: 12-20. 10.1016/S0167-6377(02)00185-2.View ArticleGoogle Scholar
- Haubold H, Hudson RR: LIAN 3.0: detecting linkage disequilibrium in multilocus data. Bioinformatics. 2000, 16: 847-848. 10.1093/bioinformatics/16.9.847.View ArticlePubMedGoogle Scholar
- Szpiech ZA, Jakobsson M, Rosenberg NA: ADZE: a rarefaction approach for counting alleles private to combinations of populations. Bioinformatics. 2008, 24: 2498-2504. 10.1093/bioinformatics/btn478.PubMed CentralView ArticlePubMedGoogle Scholar
- Taylor LH, Read AF: Determinants of transmission success of individual clones from mixed-clone infections of the rodent malaria parasite, Plasmodium chabaudi. Int J Parasitol. 1998, 28: 719-725. 10.1016/S0020-7519(98)00032-0.View ArticlePubMedGoogle Scholar
- Taylor LH, Walliker D, Read AF: Mixed-genotype infections of the rodent malaria Plasmodium chabaudi are more infectious to mosquitoes than single-genotype infections. Parasitol. 1997, 115: 121-132. 10.1017/S0031182097001145.View ArticleGoogle Scholar
- De Roode JC, Pansini R, Cheesman SJ, Helinski MEH, Huijben S, Wargo AW, Bell AS, Chan BHK, Walliker D, Read AF: Virulence and competitive ability in genetically diverse malaria infections. Proc Natl Acad Sci USA. 2005, 102: 7624-7628. 10.1073/pnas.0500078102.PubMed CentralView ArticlePubMedGoogle Scholar
- Wargo AR, de Roode JC, Huijben S, Drew DR, Read AF: Transmission stage investment of malaria parasites in response to in-host competition. Proc R Soc Biol. 2007, 274: 2629-2538. 10.1098/rspb.2007.0873.View ArticleGoogle Scholar
- Drakeley C, Sutherland C, Bousema JT, Sauerwein RW, Targett GAT: The epidemiology of Plasmodium falciparum gametocytes: weapons of mass destruction. Trends Parasitol. 2006, 22: 424-430. 10.1016/j.pt.2006.07.001.View ArticlePubMedGoogle Scholar
- Nacher M, Singhasivanon P, Silachamroon U, Treeprasertsuk S, Tosukhowong T, Vannaphan S, Gay F, Mazier D, Looareesuwan S: Decreased hemoglobin concentration, hyperparasitemia, and severe malaria are associated with increased Plasmodium falciparum gametocyte carriage. J Parasitol. 2002, 88: 97-101.View ArticlePubMedGoogle Scholar
- Paul REL, Lafond T, Mϋller-Graf CDM, Nithiuthai S, Brey PT, Koella JC: Experimental evaluation of the relationship between lethal or non-lethal virulence and transmission success in malaria parasite infections. Evol Biol. 2004, 4: 30-10.1186/1471-2148-4-30.Google Scholar
- Reece SE, Duncan AB, West SA, Read AF: Host cell preference and variable transmission strategies in malaria parasites. Proc R Soc B. 2005, 272: 511-517. 10.1098/rspb.2004.2972.PubMed CentralView ArticlePubMedGoogle Scholar
- Prugnolle F, Durand P, Jacob K, Razakandrainibe F, Arnathau C, Villarreal D, Rousset F, de Meeûs T, Renaud F: A comparison of Anopheles gambiae and Plasmodium falciparum genetic structure over space and time. Microb Infect. 2008, 10: 269-275. 10.1016/j.micinf.2007.12.021.View ArticleGoogle Scholar
- Hughes A: Positive selection and interallelic recombination at the merozoite surface antigen-1 (MSA-1) locus of Plasmodium falciparum. Mol Biol Evol. 1992, 9: 381-393.PubMedGoogle Scholar
- Gupta S, Day KP: A strain theory of malaria transmission. Parasitol Today. 1994, 10: 476-481. 10.1016/0169-4758(94)90160-0.View ArticlePubMedGoogle Scholar
- Kouyos RD, Silander OK, Bonhoeffer S: Epistasis between deleterious mutations and the evolution of recombination. Trends Ecol Evol. 2007, 22: 308-315. 10.1016/j.tree.2007.02.014.View ArticlePubMedGoogle Scholar
- Hill WG, Robertson A: The effect of linkage on limits to artificial selection. Genetical Res. 1966, 8: 269-294. 10.1017/S0016672300010156.View ArticleGoogle Scholar
- Eshel I, Feldman MW: On the evolutionary effect of recombination. Theor Popul Biol. 1970, 1: 88-100. 10.1016/0040-5809(70)90043-2.View ArticlePubMedGoogle Scholar
- Feldman MW, Otto SP, Christiansen FB: Population genetic perspectives on the evolution of recombination. Annu Rev Genet. 1996, 30: 261-295. 10.1146/annurev.genet.30.1.261.View ArticlePubMedGoogle Scholar
- Rathod PK, McErlean T, Lee PC: Variations in frequencies of drug resistance in Plasmodium falciparum. Proc Natl Acad Sci USA. 1997, 94: 9389-9393. 10.1073/pnas.94.17.9389.PubMed CentralView ArticlePubMedGoogle Scholar
- Hill AV, Yates SN, Allsopp CE, Gupta S, Gilbert SC, Lalvani A, Aidoo M, Davenport M, Plebanski M: Human leukocyte antigens and natural selection by malaria. Philos Trans R Soc Lond B Biol Sci. 1994, 346: 379-385. 10.1098/rstb.1994.0155.View ArticlePubMedGoogle Scholar
- Ali E, Mackinnon MJ, Abdel-Muhsin AA, Ahmed S, Walliker D, Babiker HA: Increased density but not prevalence of gametocytes following drug treatment of Plasmodium falciparum. Trans R Soc Trop Med Hyg. 2006, 100: 176-183. 10.1016/j.trstmh.2005.04.021.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.