Defining the relationship between Plasmodium falciparum parasite rate and clinical disease: statistical models for disease burden estimation
© Patil et al; licensee BioMed Central Ltd. 2009
Received: 3 June 2009
Accepted: 5 August 2009
Published: 5 August 2009
Clinical malaria has proven an elusive burden to enumerate. Many cases go undetected by routine disease recording systems. Epidemiologists have, therefore, frequently defaulted to actively measuring malaria in population cohorts through time. Measuring the clinical incidence of malaria longitudinally is labour-intensive and impossible to undertake universally. There is a need, therefore, to define a relationship between clinical incidence and the easier and more commonly measured index of infection prevalence: the "parasite rate". This relationship can help provide an informed basis to define malaria burdens in areas where health statistics are inadequate.
Formal literature searches were conducted for Plasmodium falciparum malaria incidence surveys undertaken prospectively through active case detection at least every 14 days. The data were abstracted, standardized and geo-referenced. Incidence surveys were time-space matched with modelled estimates of infection prevalence derived from a larger database of parasite prevalence surveys and modelling procedures developed for a global malaria endemicity map. Several potential relationships between clinical incidence and infection prevalence were then specified in a non-parametric Gaussian process model with minimal, biologically informed, prior constraints. Bayesian inference was then used to choose between the candidate models.
The suggested relationships with credible intervals are shown for the Africa and a combined America and Central and South East Asia regions. In both regions clinical incidence increased slowly and smoothly as a function of infection prevalence. In Africa, when infection prevalence exceeded 40%, clinical incidence reached a plateau of 500 cases per thousand of the population per annum. In the combined America and Central and South East Asia regions, this plateau was reached at 250 cases per thousand of the population per annum. A temporal volatility model was also incorporated to facilitate a closer description of the variance in the observed data.
It was possible to model a relationship between clinical incidence and P. falciparum infection prevalence but the best-fit models were very noisy reflecting the large variance within the observed opportunistic data sample. This continuous quantification allows for estimates of the clinical burden of P. falciparum of known confidence from wherever an estimate of P. falciparum prevalence is available.
A new global map of P. falciparum malaria endemicity for 2007 has now been published . This provides a continuous prediction of prevalence (P. falciparum parasite rate in the two up to ten year age group, Pf PR2–10, between 0–100%) for every 5 × 5 km pixel within the stable limits of P. falciparum malaria transmission . This map overcomes some of the problems of modified versions of the map created by Lysenko : it is a contemporary measure of global malaria endemicity, based on evidence from a huge repository of parasite rate surveys  and implemented in a Bayesian model-based geostatistical framework , so that the uncertainty in predictions is represented explicitly . This map therefore, provides a substantially improved spatial platform for future revision of clinical malaria burden estimates.
Since the map now provides estimates of endemicity as a continuous variable , in contrast to the historical categorical map , it becomes important to investigate the relationship between P. falciparum malaria prevalence and incidence. Here, a revised data assembly is presented to better define the continuous relationship between clinical incidence and parasite prevalence. Importantly, the uncertainty in this relationship is modelled and can, therefore, be quantified in future "cartographic" burden estimation initiatives [19, 20].
Formal literature searches for data were conducted on PubMed  with the search term: "Malaria" [Mesh] AND ("Incidence" [Mesh] OR "Epidemiology" [Mesh] OR "epidemiology" [Subheading]) AND (("1985/01/01" [PDat]:"2008/12/01" [PDat])). These searches were run for the final time on 31 December 2008. These lists were augmented with the results of previous searches  and various additional search strategies intended to identify informal literature, including direct approaches to authors. The aim was to identify all active fever surveillance surveys undertaken and reported on since 01 January 1985.
All studies were required to meet the following criteria for inclusion: (i) the study was undertaken since 1985 and thus coincidental with malaria prevalence data used in the global maps of P. falciparum endemicity ; (ii) surveys were longitudinal and community-based involving prospective active case detection (ACD) of fever cases; (iii) ACD was undertaken at a minimum frequency of fortnightly; (iv) results were presented in a way that allowed the computation of the number of cases and person years of observation; (v) all fever cases were examined for the presence of P. falciparum parasites in the peripheral blood; (vi) surveys covered all age groups in the sampled population from birth to adulthood; and (vii) surveys were undertaken over at least one complete 12 month period to account for seasonal variation, or were within a defined (by vector surveillance) period of transmission. A minimum definition of "clinical malaria" was defined as the presence of self-reported or measured fever in a subject who had coincidental P. falciparum infection of any parasite density.
Single cross-sectional surveys and surveys where communities were visited monthly and thus likely to miss disease events were not included. Daily surveys were also excluded because these extremely intensive investigations of populations were difficult to separate from the studies' direct influence on local malaria incidence. The frequency of active case detection (ACD) in included studies, therefore, varied between two days and two weeks.
The literature search for data and additional correspondence identified 103 publications and reports with potential data that were reviewed. Data from these studies were extracted and coded according to the method of case ascertainment (ACD, repeat cross-sectional surveys with or without PCD); the frequency of case detection; the case definition used for each study and the number of P. falciparum cases identified; the study period and duration and the size of the population studied. From the 103 identified sources, 61 reports fulfilled the combined inclusion criteria (See Additional File 1). Three were excluded because the survey report provided insufficient details on how the survey was conducted, a further nine were excluded because they were not undertaken across a complete calendar year, another sixteen because they were daily, monthly or cross-sectional reports, ten studies because they involved PCD only and four studies for a selection of other reasons. Fourteen surveys were included despite only providing limited details of the surveillance methods, where an informed estimate could be obtained on the frequency of surveillance from parallel literature from the same area or same author (these are indicated in the footnotes in Additional File 1).
Reports capturing more than one village population were disaggregated to represent separate observations where possible. All observations were then geo-positioned using standard techniques outlined elsewhere . In those cases where incidence could be presented for several years, every year of follow up was reported separately. The results of the data abstractions (n = 141 observations) for these epidemiological surveillance studies of P. falciparum clinical incidence are shown in Additional File 1. The data are grouped by region, country and location.
Matching to infection prevalence
A contemporary estimate of Pf PR was included with, or obtained through personal communication for, 91 of the 141 clinical incidence observations (Africa+ 16, America 14 and CSE Asia 61). These data are shown in Additional File 1 (column Pf PR). Where it was not possible to use the same report to match a congruent estimate of Pf PR, the Malaria Atlas Project database  was used to search for surveys that were matched as close as possible in time (within five years) and space (within 20 km) to the estimates of clinical incidence. This yielded 23 further matches (Africa+ 4, America 6 and CSE Asia 13). Where this was not possible, the prevalence was predicted using the same database and procedures used to generate a global map of P. falciparum endemicity for 2007 . This applied to 27 further points (Africa+ 5, America 0 and CSE Asia 22). These procedures are described in detail elsewhere  but, briefly, a Bayesian geostatistical model was constructed that could predict prevalence continuously through space and time by defining a spatio-temporal random field parameterized by a space-time covariance function that included a seasonality component and a mean function that incorporated long-term temporal trends in endemicity and covariates such as the influence of urban and rural areas. The model also incorporated a Bayesian standardization for the age range over which Pf PR surveys were conducted allowing all predictions to be made for a common 2–10 year age window. Infection prevalence estimates were therefore provided for this study that matched both the spatial location of each clinical incidence survey and the time period over which it was collected. These data are all shown in Additional File 1 (column Pf PR2–10). These prevalence predictions were also generated for all incidence studies (Africa+ 25, America 20 and CSE Asia 96) for comparison and are shown in column "Pf PR2–10 predicted". All subsequent modelling was carried out using three alternative sources of matched infection prevalence values: those from prevalence observations made during, or close in space and time to the incidence surveys only; those resulting from the geostatistical model only; or a combination of the two where the geostatistical predictions were used where suitable observations were not available.
The algebraic form of the relationship between average parasite rate and average clinical incidence for P. falciparum malaria is not known. However, prior knowledge based on epidemiological evidence does allow certain constraints to be placed on the form of plausible relationships . For example, the relationship must start at the origin (when incidence is zero, prevalence must also be zero), should be relatively smooth (because it is underpinned by a biological mechanism) and its rate of increase should slow and possibly become negative at the highest parasite rates (as the individuals that constitute the population at high endemic levels develop an immunity which enables them to manage the clinical manifestations of infection ).
Expected incidence f(p) was modelled as a function of age-adjusted parasite rate p using a Gaussian process, a very flexible probability distribution for functions. By using a Gaussian process, the overall shape of f was constrained to incorporate the prior epidemiological knowledge without imposing any particular parametric model form. A Gaussian process in this context has two components: a mean function (controlling the central tendency of the function at a given value of p) and a covariance (controlling the second-order characteristics of the function, such as its smoothness).
The Gaussian covariance model  was used to ensure that f was infinitely differentiable, meaning not rough. This smoothness was considered desirable because the biological mechanisms causing these relationships and those forms chosen by most parametric models would also be smooth. The mean function of f was modelled using the simple parametric model E[f(p)] = a(1 - exp(-bp)) where a and b are unknown and assigned uniform priors between 0 and 100, and 0.1 and 100, respectively. The Gaussian process distribution was conditioned so that f(0) = 0, in other words incidence was constrained to be zero when parasite rate was zero. Values of 0.6 and 1 were chosen a priori for the range and sill parameters of f. It would have been preferable to infer them with the other parameters, but this more flexible model was prohibitively difficult to fit. These values were chosen because they produced a range of curve shapes for f that were judged to be reasonable.
These three statements (equations 1 to 3) together formed an explicit model for the timings of individual clinical events. However, this model can easily be aggregated to obtain a model for the total number of cases observed by each study.
The influence of observation frequency on fever detection has been described elsewhere , so the time intervals of surveys were standardized to approximate weekly surveillance where required. When ACD incidence was described without accompanying passive case detection (PCD), frequency was scaled to every seven days by multiplying t j by the appropriate factor: fortnightly surveillance by 2.000 and surveillance conducted every ten, three and two days by 1.4002, 0.429, 0.286 respectively. For studies that were conducted alongside PCD, t j was unadjusted assuming missed cases would be detected passively.
It was assumed that P. falciparum infections will tend to clear within a week, so that weekly active surveillance will catch essentially all cases whereas fortnightly active surveillance will catch cases with probability 0.5. Passive surveillance, however, was assumed to catch all cases even if the interval of surveillance is greater than fortnightly. It was also assumed that surveillance that is more frequent than weekly will double-count some cases.
This likelihood model and the Gaussian process model for f, together with uniform priors for the scalar parameters with appropriate constraints (such as positivity), formed a Bayesian probability model for the data. The model was fitted using Markov chain Monte Carlo  using the open-source Bayesian analysis package PyMC . The code is freely available upon request.
If the prevalence and incidence relationship varied spatially within a given region, then the use of a single model for all areas in that region would lead to worse model fit in some areas than others. This, in turn, would be manifested as spatial dependence in the model residuals [18, 36, 37]. To investigate this possibility, sample semivariograms of the mean deviance residuals in each were examined.
Comparison of results of the predicted prevalence and incidence relationship obtained using the three alternative sources of matched Pf PR prevalence values (observed contemporarily to the incidence surveys, predicted with the geostatistical model, or a combination of the two) revealed very little difference. The decision was made, therefore, to use prevalence values predicted by the geostatistical model as the matched value for all incidence surveys, regardless of whether they had an observed prevalence value available, and these are the results presented here. The geostatistical model was preferred because prevalence values were matched to incidence surveys exactly in space and time and were age-standardized. An additional advantage was that these modelled prevalences will form the basis for future burden estimates so their use in this study provides a consistency with future work. The results obtained using the alternative matched prevalence values are available on request.
The left-hand panels show the posterior distribution of f, the expected incidence. The right-hand panels show the predictive distribution of observed incidence y for a hypothetical future survey that would sample 1000 individuals weekly for two years. These "predictive panels" are a better indication of the credible intervals that would be used in burden estimation in a small area because the "posterior panels" do not incorporate temporal volatility. The "posterior panels" predict the average incidence in a region over a period of many years, rather than in any particular year. In other words, they average out the natural temporal variation that is regularly observed from year to year in malaria incidence, whereas the "predictive panels" incorporate this variation. These considerations are explored further in the discussion.
In each region, incidence increased slowly and smoothly as a function of prevalence; reaching about 250 and 500 cases per thousand of the population per annum at parasite prevalences above 40% in the Americas and CSE Asia combined region and the Africa+ region respectively. As expected, incidence is highly variable for any given prevalence. In the Africa+ region, for example, the predicted 95% credible interval for the number of observed clinical attacks, given that 1000 individuals from a population with a parasite rate of 30% will be sampled weekly for two years, extends from less than 20 to about 1500. This range may seem huge, but it is not unrepresentative of the data: the four observations for which PR is between 10% and 25% had scaled incidences of 127.7, 53.8, 43.1 and 975.6. In addition, the scarcity of data in the Africa+ region causes relatively large parameter uncertainty. In CSE Asia and the Americas, the analogous interval extends from about 50 to more than 700. While many more data points are available in this region, they are clearly very noisy. Scaled incidence for points with parasite rates between 10% and 25% ranged from about 3 to 750.
This study provides the first example of a nonparametric Bayesian inference of a functional relationship in epidemiology, although Munch et al  used a similar approach in a comparable model selection problem in fishery science. Moreover, this work also models for the first time the continuous relationship between paired prevalence and incidence in communities.
In each region, incidence increased gradually and smoothly (as specified by the model) as a function of prevalence, but was highly variable for any given prevalence. These relationships had similar forms in the Africa+ and the combined America and CSE Asia regions. The rate of increase in prevalence in the Africa+ region was higher however, reaching levels of around 500 cases per thousand of the population per annum at prevalences above 40% compared to approximately 250 cases per thousand of the population per annum in the combined America and CSE Asia data.
There are many potential reasons as to why these relationships may show regional variation, including: differences in the dominant vector species [39, 42], the impact of drug resistance on recrudescent clinical attacks , the possible modification of P. falciparum clinical outcomes in areas of Plasmodium vivax co-infection [44, 45] and the genetic contribution to disease risks as part of regionally defined inherited blood disorders .
Effect of the time period modelled on credible intervals
A critical component of the modelling approach outlined is the incorporation of temporal volatility in incidence rate: its natural tendency to fluctuate independently of changes in prevalence. An important implication of this mechanism is illustrated in Figure 5 in which the left panels show the average incidence if a population were held at a constant parasite rate for a very long time, and the right panels show the average incidence over a year. Although it is tempting to consider the left-hand panels as illustrating the "underlying" relationship that remains after the noise in the data has been filtered out, the right-hand panels are the correct ones to associate with predictions for a given year in a single population. Intuitively, this can also be understood because the credible intervals of the right-hand panels (Figure 5) match more closely the range of the observed data. The noisy predictions reflect the empirical fact that the situation at any given time in a region may deviate significantly from any "typical" transmission pattern that conventional wisdom dictates there.
In a departure from standard epidemiological practice, the credible intervals derived capture uncertainty in the long-term relationship, as well as uncertainty expected in future observations due to temporal volatility. Rather than considering the randomness in the data to be a nuisance that obscures the true malaria dynamics, it has been modelled as an essential component of the predictive statistical method.
In addition to assumptions about the relationship between parasite rate and expected incidence, these results depend on assumptions about the temporal volatility. Firstly, it was assumed that volatility decreased with increasing average parasite rate. Examination of the posterior distribution of the volatility function r suggested that this constraint did not restrict artificially the values fitted by the model (this was not shown). Secondly, it was assumed that factors such as age and immunity that affect individuals' probability of a clinical episode independently from prevalence did not differ systematically between communities.
The model also assumed that, given average parasite rate, incidence at different times and places was independent. In other words, all spatiotemporal correlation in incidence was driven by that in the underlying parasite rate. This assumption is likely to have implications for predictions of burden over extended spatiotemporal regions and should be carefully examined when more relevant data are available. Furthermore, we have not considered the possible influence on the prevalence-incidence relationship of morbidity from other sources such as co-infection . The absence of clear spatial autocorrelation in the model residuals suggests that, if any such effects were induced by a secondary spatially varying condition, these effects were weak.
This study has focussed on describing an empirical relationship to support burden estimation initiatives but there is clearly a significant opportunity for extending mathematical modelling work [23, 48, 49] in this area to help guide thinking about plausible biological mechanisms. This study has been reliant on a series of surveys conducted by a range of authors, in a range of countries with very different objectives. It is not inconceivable that a series of planned systematic prospective studies, for example a stratified sample of new paired incidence and prevalence measures along a gradient of transmission in each continent, would reduce substantially the uncertainty associated with this relationship.
This work is informative about the nature of the relationship between incidence and prevalence but also provides an important step in enabling revisions of the global clinical burden of P. falciparum malaria. The Malaria Atlas Project [19, 20] is currently developing the computational tools needed to draw samples from the joint predictive distribution of the global endemicity surface (also known as "conditional simulations") . The statistical model presented here was developed specifically for the purpose of converting these samples to corresponding samples of the global incidence surface, and thence to samples from the predictive distribution of burden in any geographical area.
We specifically wish to thank Mohammed Borle, Verena Carrara, Iqbal Elyazar, Blaise Genton, Helen Guyatt, Chandy John, Grainne Moloney, Ivo Muller, Tabitha Mwangi, Abdisalan Noor, Tom Smith and Dejan Zurovac for age-specific clinical malaria data. EAO is funded by a Research Training Fellowship from the Wellcome Trust (#086166). SIH is funded by a Senior Research Fellowship from the Wellcome Trust (#079091) which also supports APP, PWG and CAG. RWS is a Wellcome Trust Principal Research Fellow (#079080). EO, SIH, EAO and RWS acknowledge the support of the Kenyan Medical Research Institute (KEMRI). This paper is published with the permission of the director of KEMRI. This work forms part of the output of the Malaria Atlas Project (MAP, http://www.map.ox.ac.uk), principally funded by the Wellcome Trust, U.K.
- Bruce-Chwatt LJ: Malaria in African infants and children in Southern Nigeria. Ann Trop Med Parasitol. 1952, 46: 173-200.PubMedGoogle Scholar
- Carter R, Mendis KN: Evolutionary and historical aspects of the burden of malaria. Clin Microbiol Rev. 2002, 15: 564-594. 10.1128/CMR.15.4.564-594.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Greenwood BM: Population at risk. Parasitol Today. 1990, 6: 188-10.1016/0169-4758(90)90351-4.View ArticlePubMedGoogle Scholar
- Hay SI, Guerra CA, Tatem AJ, Atkinson PM, Snow RW: Urbanization, malaria transmission and disease burden in Africa. Nat Rev Microbiol. 2005, 3: 81-90. 10.1038/nrmicro1069.PubMed CentralView ArticlePubMedGoogle Scholar
- Roca-Feltrer A, Carneiro I, Armstrong Schellenberg JR: Estimates of the burden of malaria morbidity in Africa in children under the age of 5 years. Trop Med Int Health. 2008, 13: 771-783.View ArticlePubMedGoogle Scholar
- Rowe AK, Rowe SY, Snow RW, Korenromp EL, Schellenberg JRA, Stein C, Nahlen BL, Bryce J, Black RE, Steketee RW: The burden of malaria mortality among African children in the year 2000. Int J Epidemiol. 2006, 35: 691-704. 10.1093/ije/dyl027.PubMed CentralView ArticlePubMedGoogle Scholar
- Schwartlander B: Global burden of disease. Lancet. 1997, 350: 141-142. 10.1016/S0140-6736(05)61844-2.View ArticlePubMedGoogle Scholar
- Snow RW, Craig M, Deichmann U, Marsh K: Estimating mortality, morbidity and disability due to malaria among Africa's non-pregnant population. Bull World Health Organ. 1999, 77: 624-640.PubMed CentralPubMedGoogle Scholar
- Snow RW, Craig MH, Newton CRJC, Steketee RW: The public health burden of Plasmodium falciparum malaria in Africa: deriving the numbers. Working Paper No.11, Disease Control Priorities Project. 2003, Bethesda, Maryland, U.S.A.: Fogarty International Centre, National Institutes of HealthGoogle Scholar
- Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI: The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature. 2005, 434: 214-217. 10.1038/nature03342.PubMed CentralView ArticlePubMedGoogle Scholar
- Sturchler D: How much malaria is there worldwide?. Parasitol Today. 1989, 5: 39-40. 10.1016/0169-4758(89)90188-9.View ArticleGoogle Scholar
- Gething PW, Noor AM, Gikandi PW, Ogara EAA, Hay SI, Nixon MS, Snow RW, Atkinson PM: Improving imperfect data from health management information systems in Africa using space-time geostatistics. PLoS Med. 2006, 3: e271-10.1371/journal.pmed.0030271.PubMed CentralView ArticlePubMedGoogle Scholar
- Lysenko AJ, Semashko IN: Geography of malaria. A medico-geographic profile of an ancient disease [in Russian]. Itogi Nauki: Medicinskaja Geografija. Edited by: Lebedew AW. 1968, Moscow: Academy of Sciences, USSR, 25-146.Google Scholar
- Metselaar D, Van Thiel PH: Classification of malaria. Trop Geogr Med. 1959, 11: 157-161.Google Scholar
- Hay SI, Guerra CA, Gething PW, Patil AP, Tatem AJ, Noor AM, Kabaria CW, Manh BH, Elyazar IRF, Brooker SJ, Smith DL, Moyeed RA, Snow RW: A world malaria map: Plasmodium falciparum endemicity in 2007. PLoS Med. 2009, 6: e1000048-PubMed CentralView ArticlePubMedGoogle Scholar
- Guerra CA, Gikandi PW, Tatem AJ, Noor AM, Smith DL, Hay SI, Snow RW: The limits and intensity of Plasmodium falciparum transmission: implications for malaria control and elimination worldwide. PLoS Med. 2008, 5: e38-10.1371/journal.pmed.0050038.PubMed CentralView ArticlePubMedGoogle Scholar
- Guerra CA, Hay SI, Lucioparedes LS, Gikandi PW, Tatem AJ, Noor AM, Snow RW: Assembling a global database of malaria parasite prevalence for the Malaria Atlas Project. Malar J. 2007, 6: 17-10.1186/1475-2875-6-17.PubMed CentralView ArticlePubMedGoogle Scholar
- Diggle PJ, Ribeiro PJ: Model-based geostatistics. 2007, New York: SpringerGoogle Scholar
- Hay SI: The Malaria Atlas Project (MAP). 2009, [http://www.map.ox.ac.uk]Google Scholar
- Hay SI, Snow RW: The Malaria Atlas Project: developing global maps of malaria risk. PLoS Med. 2006, 3: e473-10.1371/journal.pmed.0030473.PubMed CentralView ArticlePubMedGoogle Scholar
- Anonymous: PubMed: A service of the U.S. National Library of Medicine and the National Institutes of Health. [http://www.ncbi.nlm.nih.gov/sites/entrez]
- Snow RW, Marsh K: The consequences of reducing transmission of Plasmodium falciparum in Africa. Adv Parasitol. 2002, 52: 235-264. full_text.View ArticlePubMedGoogle Scholar
- Smith DL, Dushoff J, Snow RW, Hay SI: The entomological inoculation rate and Plasmodium falciparum infection in African children. Nature. 2005, 438: 492-495. 10.1038/nature04024.PubMed CentralView ArticlePubMedGoogle Scholar
- Gelman A, Carlin JB, Stern HS: Bayesian data analysis. Texts in Statistical Science. 2003, Boca Raton, Florida, U.S.A.: Chapman & Hall/CRC Press LLC, SecondGoogle Scholar
- MacKay DJC: Introduction to Gaussian Processes. Neural networks and machine learning. NATO advanced study institute on generalization in neural networks and machine learning, Cambridge, 4–15 August 1997. NATO. 1998, 168:Google Scholar
- Banerjee S, Carlin BP, Gelfand AE: Hierarchical modeling and analysis for spatial data. Monographs on Statistics and Applied Probability 101. 2004, Boca Raton, Florida, U.S.A.: Chapman & Hall/CRC Press LLCGoogle Scholar
- Hay SI, Myers MF, Burke DS, Vaughn DW, Endy T, Ananda N, Shanks GD, Snow RW, Rogers DJ: Etiology of interepidemic periods of mosquito-borne disease. Proc Natl Acad Sci USA. 2000, 97: 9335-9339. 10.1073/pnas.97.16.9335.PubMed CentralView ArticlePubMedGoogle Scholar
- Hilbe JM: Negative binomial regression. 2007, Cambridge: Cambridge University PressView ArticleGoogle Scholar
- Nájera JA, Kouznetsov RL, Delacollete C: Malaria epidemics. Detection and control, forecasting and prevention. WHO/MAL/98.1084. 1998, Geneva: World Health Organization, 81-Google Scholar
- Hay SI, Simba M, Busolo M, Noor AM, Guyatt HL, Ochola SA, Snow RW: Defining and detecting malaria epidemics in the highlands of western Kenya. Emerg Infect Dis. 2002, 8: 555-562. 10.3201/eid0804.010529.PubMed CentralView ArticlePubMedGoogle Scholar
- Hay SI, Were EC, Renshaw M, Noor AM, Ochola SA, Olusanmi L, Alipui N, Snow RW: Forecasting, warning, and detection of malaria epidemics: a case study. Lancet. 2003, 361: 1705-1706. 10.1016/S0140-6736(03)13366-1.PubMed CentralView ArticlePubMedGoogle Scholar
- Møller J, Waagepetersen RP: Statistical inference and simulation for spatial point processes. Monographs on Statistics and Applied Probability 100. 2004, Boca Raton, Florida, U.S.A.: Chapman & Hall/CRC Press LLCGoogle Scholar
- Snow RW, Menon A, Greenwood BM: Measuring morbidity from malaria. Ann Trop Med Parasitol. 1989, 83: 321-323.PubMedGoogle Scholar
- Gilks WR, Spiegelhalter DJ: Markov Chain Monte Carlo in practice. Interdisciplinary Statistics. 1999, Boca Raton, Florida, U.S.A.: Chapman & Hall/CRC Press LLCGoogle Scholar
- Patil A, Huard D, Fonnesbeck C: PyMC: Markov Chain Monte Carlo for Python, version 2.0. Journal of Statistical Software. 2008,http://pymc.googlecode.com/, ,Google Scholar
- Diggle PJ, Tawn JA, Moyeed RA: Model-based geostatistics. J Roy Stat Soc C-App. 1998, 47: 299-326. 10.1111/1467-9876.00113.View ArticleGoogle Scholar
- Goovaerts P: Geostatistics for natural resource evaluation. 1997, New York, U.S.A.: Oxford University PressGoogle Scholar
- Hay SI, Sinka ME, Tatem AJ, Patil AP, Guerra CA, Okara RM, Howes RE, Kabaria CK, Bangs M, Chareonviriyaphap T, Godfray HCJ, Harbach R, Hemingway J, Mbogo CM, Rogers DJ, Rubio-Palis Y: Developing global maps of the dominant Anopheles vectors of human malaria. PLoS Med. 2009,Google Scholar
- Macdonald G: Local features of malaria. The epidemiology and control of malaria. 1957, London: Oxford University Press, 63-99.Google Scholar
- Mouchet J, Carnevale P, Coosemans M, Julvez J, Manguin S, Richard-Lenoble D, Sircoulon J: Paludisme et grandes régions biogéographiques. Biodiversité du paludisme dans le monde. 2004, Montrouge, France: John Libbey EurotextGoogle Scholar
- Munch SB, Kottas A, Mangel M: Bayesian nonparametric analysis of stock-recruitment relationships. Can J Fish Aquat Sci. 2005, 62: 1808-1821. 10.1139/f05-073.View ArticleGoogle Scholar
- Mouchet J, Carnevale P, Coosemans M, Julvez J, Manguin S, Richard-Lenoble D, Sircoulon J: Biodiversité du paludisme dans le monde. 2004, Montrouge, France: John Libbey EurotextGoogle Scholar
- Talisuna AO, Bloland P, D'Alessandro U: History, dynamics, and public health importance of malaria parasite resistance. Clin Microbiol Rev. 2004, 17: 235-254. 10.1128/CMR.17.1.235-254.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Gunewardena DM, Carter R, Mendis KN: Patterns of acquired anti-malarial immunity in Sri Lanka. Mem Inst Oswaldo Cruz. 1994, 89: 63-65. 10.1590/S0074-02761994000600015.View ArticlePubMedGoogle Scholar
- Maitland K, Williams TN, Newbold CI: Plasmodium vivax and P. falciparum : biological interactions and the possibility of cross-species immunity. Parasitol Today. 1997, 13: 227-231. 10.1016/S0169-4758(97)01061-2.View ArticlePubMedGoogle Scholar
- Weatherall D, Akinyanju O, Fucharoen S, Olivieri N, Musgrove P: Inherited disorders of hemoglobin. Disease control priorities in developing countries. Edited by: Jamison DT, Breman JG, Measham AR, Alleyne G, Claeson M, Evans DB, Jha P, Mills A, Musgrove P. 2006, New York: Oxford University Press, 663-680. 2Google Scholar
- Brooker S, Clements ACA, Hotez PJ, Hay SI, Tatem AJ, Bundy DAP, Snow RW: The co-distribution of Plasmodium falciparum and hookworm among African schoolchildren. Malar J. 2006, 5: 99-10.1186/1475-2875-5-99.PubMed CentralView ArticlePubMedGoogle Scholar
- Smith DL, McKenzie FE: Statics and dynamics of malaria infection in Anopheles mosquitoes. Malar J. 2004, 3: 13-10.1186/1475-2875-3-13.PubMed CentralView ArticlePubMedGoogle Scholar
- Smith DL, McKenzie FE, Snow RW, Hay SI: Revisiting the basic reproductive number for malaria and its implications for malaria control. PLoS Biol. 2007, 5: e42-10.1371/journal.pbio.0050042.PubMed CentralView ArticlePubMedGoogle Scholar
- Balk DL, Deichmann U, Yetman G, Pozzi F, Hay SI, Nelson A: Determining global population distribution: methods, applications and data. Adv Parasitol. 2006, 62: 119-156. 10.1016/S0065-308X(05)62004-0.PubMed CentralView ArticlePubMedGoogle Scholar
- Hay SI, Noor AM, Nelson A, Tatem AJ: The accuracy of human population maps for public health application. Trop Med Int Health. 2005, 10: 1073-1086. 10.1111/j.1365-3156.2005.01487.x.PubMed CentralView ArticlePubMedGoogle Scholar
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