Mast cell activation in the skin of Plasmodium falciparum malaria patients
© Wilainam et al.; licensee BioMed Central. 2015
Received: 7 November 2014
Accepted: 16 January 2015
Published: 7 February 2015
Mast cells (MCs) play an important role in the immune response and inflammatory processes. Generally, MCs can be stimulated to degranulate and release histamine upon binding to immunoglobulin E (IgE). In malaria, MCs have been linked to immunoglobulin (Ig) E-anti-malarial antibodies. This study investigated the response of MCs in the skin of patients with Plasmodium falciparum malaria.
Skin tissue samples were examined from ten uncomplicated and 20 complicated P. falciparum malaria cases. Normal skin tissues from 29 cases served as controls. Pre- and post-treatment tissues were included. Histopathological changes of the skin were evaluated using haematoxylin and eosin stain. MCs were investigated using toluidine blue staining. The percentage of MC degranulation was compared among groups and correlated with clinical data.
MC degranulation was significantly higher in the complicated P. falciparum (43.72% ± 1.44) group than the uncomplicated P. falciparum (31.35% ± 3.29) (p <0.05) and control groups (18.38% ± 1.75), (p <0.0001). MC degranulation correlated significantly with the degree of parasitaemia (r s = 0.66, p <0.0001). Associated pathological features, including extravasation of red blood cells, perivascular oedema and leukocyte infiltration were significantly increased in the malaria groups compared with the control group (all p <0.001).
MCs in the skin dermis are activated during malaria infection, and the degree of MC degranulation correlates with parasitaemia and disease severity.
Malaria is the most serious and widespread parasitic disease in humans, occurring widely throughout the tropical areas of the world. In 2012, 207 million cases of malaria were reported, with 627,000 deaths . The process of malaria pathogenesis is very complex, mainly involving the effects of cytoadhesion and sequestration of parasitized red blood cells (PRBCs) in the vital organs  and the induction of soluble cytokines [3,4]. The major complications of severe Plasmodium falciparum malaria include cerebral malaria, pulmonary oedema, acute kidney injury, severe anaemia, bleeding, acidosis and hypoglycaemia . These complications can develop rapidly and progress to death within hours or days .
Mast cells (MCs) are pivotal effector cells in allergic diseases, and play a role in the body’s inflammatory response and immunity. These immune cells are abundant in tissues exposed to the external environment, including the skin [7,8]. MCs can be activated by various stimuli including cytokines, chemokines and neuropeptides. The biological effects of MCs depend on the release of preformed and de novo-synthesized mediators such as histamine, proteases, leukotrienes, and various cytokines . MCs are best known for immunoglobulin (Ig) E-mediated immediate-type hypersensitivity reactions . In P. falciparum infection, the significant elevation of the blood concentrations of IgE, IgE-anti-malarial antibodies and histamine have been associated with disease severity in human  and animal models . Morphologically, MCs have not been evaluated in malaria. This study aimed to investigate the response of MCs in the skin of patients with P. falciparum malaria.
Patients and normal cases
Skin biopsied specimens from P. falciparum malaria patients were classified into two groups, uncomplicated (ten cases) and complicated (20 cases) P. falciparum malaria cases, according to the available history in the clinical chart, based on WHO criteria . All P. falciparum malaria patients enrolled had no history of underlying disease. Normal skin tissues served as control cases (29 cases). All tissues were retrieved from paraffin-embedded specimens collected at the Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University. The use of leftover specimens was approved by the Ethics Committee of the Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand (MUTM 2013-044-01).
The collected skin tissues in paraffin blocks were re-embedded, sectioned at 4 μm thickness and stained with routine haematoxylin and eosin (H&E) stain for histopathological examination. MCs were evaluated using toluidine blue stain modified from Mills et al. . Briefly, after deparaffinization and hydration, the sections were immersed in 0.1% toluidine blue working solution for 5–10 min and washed in distilled water, with three changes. The tissues were then dehydrated quickly through 90%, and two changes of absolute ethanol. Sections were then cleared in xylene, and mounted with mounting medium.
Evaluation of MCs
MCs (both intact and degranulated forms) were quantified in two areas, the upper dermis (papillary layer) and the lower dermis (reticular and subcutaneous fatty layers). MCs were counted in 15 fields each, at the areas adjacent to the blood vessels, and in the connective tissues, under high-power magnification (400×). For specimens with a small counting area, all MCs were evaluated before the percentage of degranulated MCs was calculated. Parenchymatous changes, including extravasation of RBCs, perivascular oedema, and the presence of leukocytes, were quantified and presented as percentages.
Differences among the three groups were analysed using the Kruskal-Wallis test, and expressed as geometric means ± standard error of the mean (SEM). The Mann–Whitney U test was used to compare between malaria groups, and between pre- and post- treatment data. The Statistical Package for the Social Sciences (SPSS) version 11.0 software (SPSS Chicago, IL, USA) was used, and a value of p <0.05 was deemed statistically significant.
Clinical data of malaria patients
Clinical data of Plasmodium falciparum malaria
Uncomplicated* P. falciparum malaria ( n = 5 )
Complicated P. falciparum malaria ( n = 20 )
Gender (male: female)
Days of fever (p = 0.41)
3.6 ± 0.98
4.45 ± 0.44
Admission parasite count/μL (p = 0.51)
52,638 ± 12,990
181,315 ± 55,314
WBC (×109/L) (p = 0.041)
5.82 ± 1.24
9.61 ± 0.79
Hb (g/dL) (p = 0.17)
12.70 ± 1.35
11.10 ± 0.56
Platelets (×109/L) (p = 0.20)
50.60 ± 7.98
70.85 ± 28.69
BUN (ml/dL) (p = 0.51)
24.84 ± 4.86
34.96 ± 5.70
Creatinine (ml/dL) (p = 0.73)
1.06 ± 0.11
2.21 ± 0.70
TB (mg/dL) (p = 0.01)
1.91 ± 0.34
5.88 ± 1.14
DB (mg/dL) (p = 0.0073)
0.56 ± 0.23
3.34 ± 0.80
AST (IU/L) (p = 0.0012)
25.40 ± 3.56
112.0 ± 21.50
ALT (IU/L) (p = 0.0039)
21.80 ± 5.23
90.85 ± 15.19
Evaluation of degranulated mast cells
Histopathologic changes in the skin of malaria patients
MCs have been implicated in the inflammatory response and allergic process by releasing various cytokines, associated with IgE-mediated immediate-type hypersensitivity reactions [9,15]. This study is the first to investigate the response of MCs in the skin tissues of P. falciparum malaria-infected patients. MCs were localized mostly in the papillary layer, which correlated with blood vessel density and contributed to an immediate defense against stimuli . MC degranulation is a characteristic pattern of immunological and morphological changes after activation . Generally, an increase in degranulated MCs can result in MC activation syndrome (MCAS). The syndrome includes a range of manifestations, involving the skin, gastrointestinal, cardiovascular, respiratory, and neurological system . In malaria infection, studies have shown that IgE level is associated with malaria severity [19,20]. In addition, malaria antigens have been reported to activate macrophages and monocytes to produce various cytokines, such as tumour necrosis factor (TNF)-alpha [20,21] and interleukin-1 beta . These cytokines, which are classified as MC secretagogues, may activate MCs through FcεRI receptors. The increased level of these cytokines is related to malaria severity, and is hypothesized to affect the response of MCs. MCs provide a potential source of TNF during the early phase of infection. In an animal malaria model, a study showed an association between MCs and the elevation of serum TNF, which could contribute to malaria protection . Increases in the number of MCs, and MC degranulation, have been documented in diseases such as renal amyloidosis , interstitial lung fibrosis , gastrointestinal disease  and myocardial infarction . The changes correlated with pathological alterations, and are relevant to MC mediators.
Artemisinin derivatives were the drugs of choice used to treat all malaria cases. A previous report has documented that artemisinin can decrease MC degranulation by blocking IgE-induced MC degranulation in an anaphylactic animal model . However, in this study, the number of MC degranulations remained elevated at seven days post-treatment. Thus, artemisinin may not prevent MC degranulation in malaria in vivo. Quinine and tetracycline have also been reported to cause MC degranulation . Mefloquine was recently reported to function as an anti-mast cell agent, inducing apoptosis through a granule-mediated pathway . The effects of anti-malarial drugs on mast cell degranulation need to be further investigated to determine the physiological action and pathways involved. Moreover, some antibiotics, including penicillins, cephalosporins, sulphonamides, as well as some antiepileptic drugs can cause MC degranulation by acting through IgE receptors on MCs, triggering degranulation .
Changes in the number of MCs have been reported in wound healing , keloid formation , chronic inflammation , parasitic infestation , urticaria, atopic eczema, lichen planus, psoriasis, pretibial myxedema, scleroderma, neural tumour and mycosis fungoides . The factors involved in the increased number of MCs in such conditions remain unclear . In addition, percentage MC degranulation correlated positively with initial parasite count (r s = 0.66, p <0.0001), indicating that MCs are activated in severe P. falciparum malaria. Normally, MCs can undergo repeated rounds of degranulation and regranulation [35-38]. An in vitro human lung MC culture showed late recovery (18–48 hr) from IgE-induced MC degranulation . In addition, mouse MCs recovered from degranulation at 30 min after activation . However, another study reported MCs regenerated from horseradish peroxidase induced MC degranulation after six weeks . At day 7 post-treatment, MC degranulation of uncomplicated (day 0 = 31.35% ± 3.29, day 7 = 23.93% ± 1.07, p = 0.091) and complicated P. falciparum (day 0 = 43.72% ± 1.44, day 7 = 40.32 ± 1.81%, p = 0.349) remained elevated. The process of MC regranulation at day 7 was not observed in the study, indicating that MC regranulation in the skin of P. falciparum is a delayed response (over seven days).
Parenchymatous changes, including RBC extravasation, perivascular oedema and leukocytic infiltration were significantly increased in the malaria groups. It can be speculated that RBC extravasation and perivascular oedema are secondary to endothelial cell damage, following cyto-adhesion and sequestration of PRBCs, and the local effects of MC cytoplasmic granules, such as TNF, histamine, heparin, and proteases . In addition, histamine, the major MC mediator in malaria, causes increased vascular permeability and subsequent extensive vascular damage to endothelial cells . Inflammatory reactions can also be related to immune responses during malaria infection.
MC activation and degranulation are observed in the skin dermis of severe P. falciparum malaria patients. In addition, the number of MCs and the degree of degranulation correlate with parasitaemia and disease severity. Associated histopathological changes in the dermis during malaria infection include extravasation of RBCs, perivascular oedema and leukocyte infiltration around the blood vessels.
We thank all of the staff at the Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Thailand for their support throughout this study. The page charge of this manuscript is supported by the Faculty of Tropical Medicine, Mahidol University, Thailand.
- WHO. World malaria report 2013. Geneva, Switzerland: World Health Organization; 2013.Google Scholar
- Pongponratn E, Turner GD, Day NP, Phu NH, Simpson JA, Stepniewska K, et al. An ultrastructural study of the brain in fatal Plasmodium falciparum malaria. Am J Trop Med Hyg. 2003;69:345–59.PubMedGoogle Scholar
- Mazier D, Nitcheu J, Idrissa-Boubou M. Cerebral malaria and immunogenetics. Parasite Immunol. 2000;22:613–23.View ArticlePubMedGoogle Scholar
- Clark IA, Rockett KA. The cytokine theory of human cerebral malaria. Parasitol Today. 1994;10:410–2.View ArticlePubMedGoogle Scholar
- World Health Organization, Communicable Diseases Cluster. Severe falciparum malaria. Trans R Soc Trop Med Hyg. 2000;94 Suppl 1:S1–90.View ArticleGoogle Scholar
- Trampuz A, Jereb M, Muzlovic I, Prabhu RM. Clinical review: severe malaria. Crit Care. 2003;7:315–23.View ArticlePubMed CentralPubMedGoogle Scholar
- Galli SJ, Maurer M, Lantz CS. Mast cells as sentinels of innate immunity. Curr Opin Immunol. 1999;11:53–9.View ArticlePubMedGoogle Scholar
- Gordon JR, Galli SJ. Mast cells as a source of both preformed and immunologically inducible TNF-alpha/cachectin. Nature. 1990;346:274–6.View ArticlePubMedGoogle Scholar
- Burd PR, Rogers HW, Gordon JR, Martin CA, Jayaraman S, Wilson SD, et al. Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines. J Exp Med. 1989;170:245–57.View ArticlePubMedGoogle Scholar
- Segal DM, Taurog JD, Metzger H. Dimeric immunoglobulin E serves as a unit signal for mast cell degranulation. Proc Natl Acad Sci U S A. 1977;74:2993–7.View ArticlePubMed CentralPubMedGoogle Scholar
- Enwonwu CO, Afolabi BM, Salako LO, Idigbe EO, Bashirelah N. Increased plasma levels of histidine and histamine in falciparum malaria: relevance to severity of infection. J Neural Transm. 2000;107:1273–87.View ArticlePubMedGoogle Scholar
- Maegraith B, Fletcher A. The pathogenesis of mammalian malaria. Adv Parasitol. 1972;10:49–75.View ArticlePubMedGoogle Scholar
- Mills J, Strickland G, Paterson JC. The validity of tissue mast-cell counts in postmortem material. AMA Arch Pathol. 1958;66:330–4.PubMedGoogle Scholar
- MacCara ME. Extravasation: a hazard of intravenous therapy. Drug Intell Clin Pharm. 1983;17:713–7.PubMedGoogle Scholar
- Rivera J, Gilfillan AM. Molecular regulation of mast cell activation. J Allergy Clin Immunol. 2006;117:1214–25. quiz 1226.View ArticlePubMedGoogle Scholar
- Cheng C, Ng DS, Chan TK, Guan SP, Ho WE, Koh AH, et al. Anti-allergic action of anti-malarial drug artesunate in experimental mast cell-mediated anaphylactic models. Allergy. 2013;68:195–203.View ArticlePubMedGoogle Scholar
- Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev. 1997;77:1033–79.PubMedGoogle Scholar
- Frieri M, Patel R, Celestin J. Mast cell activation syndrome: a review. Curr Allergy Asthma Rep. 2013;13:27–32.View ArticlePubMedGoogle Scholar
- Perlmann H, Helmby H, Hagstedt M, Carlson J, Larsson PH, Troye-Blomberg M, et al. IgE elevation and IgE anti-malarial antibodies in Plasmodium falciparum malaria: association of high IgE levels with cerebral malaria. Clin Exp Immunol. 1994;97:284–92.View ArticlePubMed CentralPubMedGoogle Scholar
- Perlmann P, Perlmann H, Flyg BW, Hagstedt M, Elghazali G, Worku S, et al. Immunoglobulin E, a pathogenic factor in Plasmodium falciparum malaria. Infect Immun. 1997;65:116–21.PubMed CentralPubMedGoogle Scholar
- Taverne J, Bate CA, Kwiatkowski D, Jakobsen PH, Playfair JH. Two soluble antigens of Plasmodium falciparum induce tumor necrosis factor release from macrophages. Infect Immun. 1990;58:2923–8.PubMed CentralPubMedGoogle Scholar
- Pichyangkul S, Saengkrai P, Webster HK. Plasmodium falciparum pigment induces monocytes to release high levels of tumor necrosis factor-alpha and interleukin-1 beta. Am J Trop Med Hyg. 1994;51:430–5.PubMedGoogle Scholar
- Furuta T, Kikuchi T, Iwakura Y, Watanabe N. Protective roles of mast cells and mast cell-derived TNF in murine malaria. J Immunol. 2006;177:3294–302.View ArticlePubMedGoogle Scholar
- Toth T, Toth-Jakatics R, Jimi S, Takebayashi S. Increased density of interstitial mast cells in amyloid A renal amyloidosis. Mod Pathol. 2000;13:1020–8.View ArticlePubMedGoogle Scholar
- Carroll NG, Mutavdzic S, James AL. Increased mast cells and neutrophils in submucosal mucous glands and mucus plugging in patients with asthma. Thorax. 2002;57:677–82.View ArticlePubMed CentralPubMedGoogle Scholar
- Ramsay DB, Stephen S, Borum M, Voltaggio L, Doman DB. Mast cells in gastrointestinal disease. Gastroenterol Hepatol (N Y). 2010;6:772–7.Google Scholar
- Laine P, Kaartinen M, Penttila A, Panula P, Paavonen T, Kovanen PT. Association between myocardial infarction and the mast cells in the adventitia of the infarct-related coronary artery. Circulation. 1999;99:361–9.View ArticlePubMedGoogle Scholar
- Shipley D, Ormerod AD. Drug-induced urticaria. Recognition and treatment. Am J Clin Dermatol. 2001;2:151–8.View ArticlePubMedGoogle Scholar
- Paivandy A, Calounova G, Zarnegar B, Ohrvik H, Melo FR, Pejler G. Mefloquine, an anti-malaria agent, causes reactive oxygen species-dependent cell death in mast cells via a secretory granule-mediated pathway. Pharmacol Res Perspect. 2014;2:e00066.View ArticlePubMed CentralPubMedGoogle Scholar
- Wichmann BE. The mast cell count during the process of wound healing; an experimental investigation on rats. Acta Pathol Microbiol Scand Suppl. 1955;Suppl 108:1–35.PubMedGoogle Scholar
- Smith CJ, Smith JC, Finn MC. The possible role of mast cells (allergy) in the production of keloid and hypertrophic scarring. J Burn Care Rehabil. 1987;8:126–31.View ArticlePubMedGoogle Scholar
- Metz M, Grimbaldeston MA, Nakae S, Piliponsky AM, Tsai M, Galli SJ. Mast cells in the promotion and limitation of chronic inflammation. Immunol Rev. 2007;217:304–28.View ArticlePubMedGoogle Scholar
- Arizono N, Matsuda S, Hattori T, Kojima Y, Maeda T, Galli SJ. Anatomical variation in mast cell nerve associations in the rat small intestine, heart, lung, and skin. Similarities of distances between neural processes and mast cells, eosinophils, or plasma cells in the jejunal lamina propria. Lab Invest. 1990;62:626–34.PubMedGoogle Scholar
- Nomura I, Katsunuma T, Matsumoto K, Iida M, Tomita H, Tomikawa M, et al. Human mast cell progenitors in peripheral blood from atopic subjects with high IgE levels. Clin Exp Allergy. 2001;31:1424–31.View ArticlePubMedGoogle Scholar
- Kobayasi T, Asboe-Hansen G. Degranulation and regranulation of human mast cells. An electron microscopic study of the whealing reaction in urticaria pigmentosa. Acta Derm Venereol. 1969;49:369–81.PubMedGoogle Scholar
- Dvorak AM, Schleimer RP, Lichtenstein LM. Morphologic mast cell cycles. Cell Immunol. 1987;105:199–204.View ArticlePubMedGoogle Scholar
- Dvorak AM, Schleimer RP, Schulman ES, Lichtenstein LM. Human mast cells use conservation and condensation mechanisms during recovery from degranulation. In vitro studies with mast cells purified from human lungs. Lab Invest. 1986;54:663–78.PubMedGoogle Scholar
- Burwen SJ. Recycling of mast cells following degranulation in vitro: an ultrastructural study. Tissue Cell. 1982;14:125–34.View ArticlePubMedGoogle Scholar
- Dvorak AM, Schleimer RP, Lichtenstein LM. Human mast cells synthesize new granules during recovery from degranulation. In vitro studies with mast cells purified from human lungs. Blood. 1988;71:76–85.PubMedGoogle Scholar
- Xiang Z, Block M, Lofman C, Nilsson G. IgE-mediated mast cell degranulation and recovery monitored by time-lapse photography. J Allergy Clin Immunol. 2001;108:116–21.View ArticlePubMedGoogle Scholar
- Shin J, Kang HS, Chung IH, Lew JD, Lee YD, Cho BP, et al. An electron microscopic study on mast cell degranulation and regeneration in rats. Yonsei Med J. 1986;27:30–40.View ArticlePubMedGoogle Scholar
- Lindsberg PJ, Strbian D, Karjalainen-Lindsberg ML. Mast cells as early responders in the regulation of acute blood–brain barrier changes after cerebral ischemia and hemorrhage. J Cereb Blood Flow Metab. 2010;30:689–702.View ArticlePubMed CentralPubMedGoogle Scholar
- Maegraith BG, Onabanjo AO. The effects of histamine in malaria. Br J Pharmacol. 1970;39:755–64.View ArticlePubMed CentralPubMedGoogle Scholar
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