Operational strategies of anti-malarial drug campaigns for malaria elimination in Zambia’s southern province: a simulation study
© Stuckey et al. 2016
Received: 3 December 2015
Accepted: 1 March 2016
Published: 9 March 2016
Malaria elimination requires reducing both the potential of mosquitoes to transmit parasites to humans and humans to transmit parasites to mosquitoes. To achieve this goal in Southern province, Zambia a mass test and treat (MTAT) campaign was conducted from 2011–2013 to complement high coverage of long-lasting insecticide-treated nets (LLIN). To identify factors likely to increase campaign effectiveness, a modelling approach was applied to investigate the simulated effect of alternative operational strategies for parasite clearance in southern province.
OpenMalaria, a discrete-time, individual-based stochastic model of malaria, was parameterized for the study area to simulate anti-malarial drug administration for interruption of transmission. Simulations were run for scenarios with a range of artemisinin-combination therapies, proportion of the population reached by the campaign, targeted age groups, time between campaign rounds, Plasmodium falciparum test protocols, and the addition of drugs aimed at preventing onward transmission. A sensitivity analysis was conducted to assess uncertainty of simulation results. Scenarios were evaluated based on the reduction in all-age parasite prevalence during the peak transmission month one year following the campaign, compared to the currently-implemented strategy of MTAT 19 % population coverage at pilot and 40 % coverage during the first year of implementation in the presence of 56 % LLIN use and 18 % indoor residual spray coverage.
Simulation results suggest the most important determinant of success in reducing prevalence is the population coverage achieved in the campaign, which would require more than 1 year of campaign implementation for elimination. The inclusion of single low-dose primaquine, which acts as a gametocytocide, or ivermectin, which acts as an endectocide, to the drug regimen did not further reduce parasite prevalence one year following the campaign compared to the currently-implemented strategy. Simulation results indicate a high proportion of low-density infections were missed by rapid diagnostic tests that would be treated and cleared with mass drug administration (MDA).
The optimal implementation strategy for MTAT or MDA will vary by background level of prevalence, by rate of infections imported to the area, and by ability to operationally achieve high population coverage. Overall success with new parasite clearance strategies depends on continued coverage of vector control interventions to ensure sustained gains in reduction of disease burden.
KeywordsMalaria Modelling Elimination Plasmodium falciparum Primaquine Ivermectin Zambia
Malaria elimination requires reducing both the potential of mosquitoes to transmit parasites to humans and humans to transmit parasites to mosquitoes, including reaching humans with asymptomatic infections. With the backdrop of several years of high coverage of long-lasting insecticide-treated mosquito nets (LLINs), many countries are looking to reorient their malaria programmes from control to elimination, including the adoption of additional strategies for programme implementation and surveillance.
Zambia has a long history of malaria control efforts dating back to the 1920s with the mining sector in the copperbelt . With the advent of the Roll Back Malaria initiative in 1998, Zambia began to introduce additional insecticide-treated bed nets (ITNs) as part of small-scale delivery programmes targeting vulnerable populations. Following initiation of malaria funding from the Global Fund for AIDS, Tuberculosis and Malaria and other partners, a scaling up of key malaria interventions, including mass distribution of ITNs, renewed efforts to increase IRS coverage, artemisinin-combination therapy (ACT) for anti-malarial treatment, and rapid diagnostic tests for case management began to reach communities by 2005. This scaling up for impact (SUFI) approach contributed to an observed 63 % decrease in Plasmodium falciparum prevalence in ages 2–9 and a 22 % decrease in malaria deaths . In response to the need to plan next steps to accelerate the goal of malaria elimination the Zambia national malaria control centre (NMCC) in partnership with PATH MACEPA is conducting an evaluation of strategies for anti-malarial drug campaigns. These strategies are designed to complement the existing high coverage of vector control interventions and aim to contribute to a marked reduction of community level malaria. In addition to the 2011–2013 implementation strategy of mass test and treat (MTAT) targeting all ages with three rounds of artemether-lumefantrine (AL)  an additional set of operational and chemotherapeutic options are currently under evaluation . These changes to the design include replacing AL with another ACT, dihydroartemisinin + piperaquine (DHAP), which clears asexual blood stages of current infections and has a longer prophylactic period against future malaria infection , as well as use of single low-dose (SLD) primaquine  and/or ivermectin  to stop onward transmission to humans.
Primaquine is a gametocytocidal drug that reduces the duration of late-stage P. falciparum gametocytes, the transmissible stage of the parasite from human to mosquito, and therefore has the potential to reduce malaria transmission . The World Health Organization (WHO) currently recommends the use of SLD primaquine in addition to an ACT for all patients with parasitologically-confirmed P. falciparum malaria to aid in malaria elimination and to contain the spread of artemisinin resistance . However, this use of primaquine is not currently widespread due to the limited availability of appropriate dosing regimens for young children and some residual confusion regarding the safety of SLD versus standard higher dosing of primaquine in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Clinical trials to determine the lowest possible dose effective at clearing gametocytes are in process in a number of locations considering the inclusion of the drug in elimination strategies including Uganda , The Gambia , and Mali .
Ivermectin is an anthelmintic drug currently used for control of onchocerciasis and lymphatic filariasis. It also has endectocidal properties reducing the sporogony  and survivorship  of mosquitoes that take a blood meal from humans with the drug in their system. Studies indicate that mass drug administration (MDA) with ivermectin has the potential to assist in the interruption of malaria transmission [15, 16], and has the potential to combine malaria elimination efforts with other helminth-based diseases considered for elimination.
OpenMalaria is a stochastic individual-based model of malaria that has been used to address questions relevant to malaria epidemiology, vaccine development , cost-effectiveness of different combinations of malaria control interventions , and vector control technologies . In order to assist the National Malaria Control Programme and implementing partners in Zambia with model-based guidance on community-based treatment strategies, OpenMalaria was employed to simulate the effectiveness of changing the operational strategy of the malaria testing and treatment campaign. This paper presents the simulation results of MTAT with AL, MTAT with DHAP (with or without ivermectin), and MDA with DHAP (with or without ivermectin and/or SLD primaquine).
Alternative strategies and drug interventions against malaria parasites and the mosquito vector were evaluated using OpenMalaria. OpenMalaria combines stochastic individual-based simulation models for malaria in humans with a periodically-forced deterministic model for malaria in mosquitoes [20–23]. The models have been fitted to multiple field data sets and the models for malaria in humans include demography, acquired immunity and super-infection, variations in parasite densities and infectiousness to mosquitoes and the clinical effects of malaria. The model for malaria in mosquitoes includes multiple mosquito species, varying vector behaviour and non-human hosts [20–23]. The model platform consists of an ensemble of fourteen model variants that allow different assumptions such as heterogeneity in exposure and decay of immunity to help quantify the effects of uncertainty in model formulation . Details of the model and simulation set up can be found in the references above.
Baseline scenario parameterization
This baseline scenario was simulated on a population of 10,000 individuals with Zambia’s population age structure . Simulation results from the baseline scenario were validated against observed rapid diagnostic test (RDT) positivity rates by facility catchment area following the scale up of the MTAT programme using AL . Results were stratified by baseline parasite prevalence, taking into account variations in transmission intensity.
Experiment design and analysis
Experiment design for mass test and treat (MTAT) with artemether-lumefantrine (AL)
Target ages receiving the campaign
All ages, children under 5, children 5–14, adults 25–49
Time between MTAT delivery rounds (total of three rounds)
2, 3, 4, 8 weeks
Proportion of the population reached per round by the MTAT campaign
0, 40, 72, 100 %
Proportion of the population who fully adhere to the AL drug regimen
50, 75, 100 %
Experiment design for campaign implementation with dihydroartemisinin–piperaquine (DHAP)
Population reached by MTAT campaign
Population reached by MDA campaign
Ivermectin duration of effectiveness
Single low-dose (SLD) primaquine half-life
Campaign implementation with DHAP
40, 72 %
40, 72 %
DHAP + ivermectin
40, 72 %a
40, 72 %
2, 4, 7 days
DHAP + SLD primaquine
40, 72 %
12, 48, 72 h
DHAP + ivermectin + SLD primaquine
40, 72 %
A sensitivity analysis was conducted to investigate the sensitivity of the intervention to changes in factors likely to influence the effect of the specific intervention or strategy. This includes the proportion of the population who fully adhere to the AL drug regimen (Table 1), and the duration of the effects of ivermectin and SLD primaquine (Table 2). In addition, each of the scenarios described above were simulated over fourteen model variants with varied assumptions about malaria epidemiology to account for model uncertainty , and three random seeds to account for the effects of stochasticity.
Intervention scenarios were evaluated based on the simulated reduction in mean all-age parasite prevalence taken over the 14 model variants and three random seeds (mean parasite prevalence) in the peak month of malaria transmission one year following the intervention compared to the baseline scenario.
With MTAT implementation, an ACT is only given to individuals with a positive RDT result; thus, the sensitivity and specificity of the RDT establish the likely proportion of which individuals will test positive according to parasite density of the infection, and which individuals will be infected but test negative. These will typically be individuals with lower parasite density who may also have a lower likelihood of transmitting to mosquitoes. In scenarios including ivermectin with MTAT, while ACT is only given to individuals who test positive, ivermectin is given to every individual reached by the campaign, regardless of the result of their malaria test. This is because ivermectin is not a therapeutic drug for malaria treatment, but rather acts as an endectocide that is toxic to mosquitoes that bite an individual with the drug in their system. With MDA implementation, the drugs are given to everyone. However, this is moderated by the population coverage of the intervention; some people are reached and others are not, and an individual has the same independent likelihood of receiving the intervention in each delivery round.
LLIN deterrency and pre- and post-prandial killing of malaria vector mosquitoes were parameterized using experimental hut data, as described in Briët et al. . LLINs are assumed to decay over time at different rates for their physical state, chemical state, and attrition from circulation. IRS deterrency and post-prandial killing are assumed to decay over time with an exponential function and a half-life of 4 months. Both LLINs and IRS are assumed to only affect the proportion of indoor biting and resting mosquitoes. Additional details of the model description and parameterization of vector control interventions are described elsewhere [19, 35].
MTAT with AL strategy used in 2011–2013
Changing operational strategy of MTAT campaign
Effect of changing operational strategy compared to a baseline strategy of three rounds mass test and treat with artemether-lumefantrine at 40 % coverage per round of all ages of the population with 60 days between rounds
% Change in median parasite prevalence (IQR), by pre-intervention mean parasite prevalence
Change drug (%)
Targeting specific age groups for the MTAT intervention, as opposed to reaching all-ages as in the current MTAT strategy, resulted in an increase in prevalence (reduced efficacy) in all prevalence categories. This is because a smaller proportion of the population is reached, even though this proportion of the population may have a higher mean parasite prevalence. Unlike other operational strategy changes, changing the time between campaign rounds to 30 days or less showed a greater simulated benefit (reduction of mean parasite prevalence) in the higher-prevalence areas, although this benefit decreases as the prevalence level in the population decreases.
From MTAT to MDA
Simulation results suggest that the most important determinant of success in reducing prevalence is campaign coverage (Fig. 5; Table 3), with a greater effect in areas of higher all-age parasite prevalence. However, in these simulated scenarios, even when achieving high coverage with MDA in areas with a pre-intervention all-age parasite prevalence of less than 10 %, complete elimination will require more than 1 year of campaign implementation. Indeed, preliminary findings from the Southern province MDA intervention trials suggest these strategies are able to reduce parasite prevalence in areas of 8–0.5 %; not elimination, but close enough to warrant moving on to surveillance-based interventions.
Results of changing drug and testing protocol for anti-malarial drug distribution compared to a strategy of three rounds mass test and treat with artemether-lumefantrine at 72 % coverage per round of all ages of the population with 60 days between rounds
% Change in median parasite prevalence (IQR) compared to baseline strategy, by pre-intervention mean parasite prevalence
Baseline intervention strategy
MTAT with DHAP
MTAT with DHAP + ivermectin
MDA with DHAP
MDA with DHAP + ivermectin
MDA with DHAP + SLD primaquine
MDA with DHAP + ivermectin + SLD primaquine
MTAT with AL, 72 % coverage, 60 day frequency between rounds, targeting all ages
In an era where vector control strategies have been able to greatly reduce disease burden across the malaria endemic world, many countries are now evaluating how to transition programmes from control to elimination. Mass distribution of anti-malarials, with or without a diagnostic, are under consideration in many settings to markedly reduce the human parasite reservoir to allow a shift to case detection via surveillance as an intervention. Results of this study are consistent with both modelling and empirical evidence that has shown implementation of drug-based strategies to be effective within a transmission season in settings where sufficient coverage of the population can be achieved . Yet, recent studies on screen and treat for strategies targeting the whole community  and specific risk groups  have been unable to show sustained impact. This study examined how the OpenMalaria platform, parameterized according to the malaria transmissions dynamics in southern Zambia, can assist the Zambia NMCC to increase the effectiveness of these planned interventions through changes in implementation design.
Results suggest the optimal implementation strategy for deployment of community-based anti-malarial treatments requires a consistent approach addressing the variation in baseline malaria prevalence, rate of imported infections, and the achievement of high coverage in the population. Simulations conducted for this study suggest that an area such as Southern province with heterogeneous transmission and an uncertain infection importation rate, drug-based interventions based on detecting infections using current RDTs will not be sufficient to interrupt transmission. Indeed, as observed in the field study, there was no difference detected at follow-up in parasite prevalence between MTAT intervention and control in either high or low transmission areas . In this setting, a high proportion of low-density infections will be missed by RDTs that would be treated and cleared with MDA.
Simulation results suggest the most important determinant of success in reducing prevalence is the coverage of the population achieved in the campaign. The Southern province field study was designed to show how much impact would be achieved after 1 year of campaign implementation in order to move towards surveillance as an intervention by focal MDA (fMDA) and case investigation. However, even with high coverage with MDA in areas with a pre-intervention all-age parasite prevalence of less than 10 %, simulations suggest that elimination will require more than 1 year of campaign implementation. The inclusion of SLD primaquine (a gametocytocide), and ivermectin (an endectocide), to the drug regimen did not further reduce parasite prevalence within this 1 year post-campaign. It is important to note that mean parasite prevalence measured at earlier time points will yield different results, as the greatest effects will be seen several months post-campaign. Simulation of ACT regimens in a generic setting have been investigated elsewhere, both with and without the option of including SLD primaquine and/or ivermectin [39, 40]. The simulation results presented here are less optimistic about the additional added benefit of PQ and ivermectin in interrupting transmission, although, importantly, the results are evaluated at different time points.
Success of anti-malarial drug administration campaigns depends on the sustained high coverage of vector control interventions. Zambia has been able to achieve high rates of household LLIN ownership and has steadily increased its within-household availability of LLINs through successive mass distribution campaigns. However, LLINs must be used or at least deployed in the household for their full impact to be attributable. Improving simulation estimates by including more direct measures of full coverage and usage of LLINs may yield greater overall reductions in predicted parasite prevalence in combination with the anti-malarial campaigns. Similarly with IRS, improved strategies to target the application of insecticides could be evaluated for specific benefits. Ensuring that transmitting mosquitoes are susceptible to IRS chemicals, that IRS is prioritized to transmission areas, and that full coverage of targeted areas is achieved will maximize the contribution of this intervention where drug strategies are attempted.
Planning for elimination scenarios: limitations and considerations
In the process of this simulation, we have identified key aspects of both the intervention tools and their delivery; improved data from the field may help in the future to improve the model and its ability to predict outcomes. As noted above, intervention coverage is a critical variable for these population-wide treatment efforts and intervention programming should improve on the quality of the coverage data. For example, most coverage estimates only examine the coverage of the household members in households that were reached; a more accurate estimation of the population denominator and coverage could perhaps be achieved by comparing satellite imaging of houses to actual field geo-position data of which houses were reached by a campaign.
Analysis was conducted with the end-point measurement of reduction in parasite prevalence. This is, indeed, an easily-measured quantity in the field. However, future applications of the OpenMalaria model in the study area should also include an end-point measurement that corresponds to potential vector control interventions, including ivermectin. The goal of ivermectin is not to reduce prevalence, but to reduce transmission. Further analysis measuring reduction in EIR or probability of interrupting transmission would be a more useful way of evaluating the impact of this intervention. In addition to the clear need for direct application of field-measurable quantities, it would be helpful to have a standard definition for defining interruption of transmission for model outputs that is operationally relevant to programme settings. Ideally this would include identifying the number of cases per week or per month in a given health facility catchment area that the health system would be able to handle.
The model has numerous limitations that are inherent in the introduction of many assumptions required for model parameterization. For example, the modelled analysis included a sensitivity analysis for different levels of adherence to AL with the assumption that the majority of non-adherers missed the final dose in the six-dose regimen, with results showing little impact on overall effectiveness. However, non-adherence would have a far greater impact on the cure rate of DHAP if a single dose of this three-dose regimen is missed , something not included in this experiment.
The field implementation targeted health facility catchment areas for complete campaign coverage, while the simulation coverage parameterization and analysis is applied to the general population without explicit geographical limits. While model parameterization assumes an individual has the same independent likelihood of receiving the intervention, there are some groups that have a higher risk of being infected. These groups, including adults aged 25–49 that are more highly mobile than the rest of the population, were targeted in the simulation study design by varying the age groups targeted by the campaign. However, because these groups may be less likely to be reached during a general campaign, this higher risk is not captured in the baseline scenario of the model and may affect the impact of the simulated interventions even if a certain level of coverage is reached for the population as a whole.
The model parameterization assumes a conservative value for AL and DHAP prophylaxis based on modelling pharmacokinetics . However, a recent publication of post-treatment prophylaxis of anti-malarials suggests both a shorter duration of protection and a greater difference between the duration of protection between AL and DHAP in people over 5 years of age . This greater difference in prophylaxis benefit would affect the simulation results by increasing the additional transmission prevented by DHAP as compared to AL.
For the ivermectin analysis, the model assumes a common effect of ivermectin no matter the age of mosquito post-emergence, which has been shown to be different in trial settings . Several studies  have found a higher mortality rate in mosquitoes exposed to ivermectin than the 85 % assumed in this experiment; this could indicate the experiment design underestimates the effect.
The model assumes a constant rate of yearly case importation (20 cases per 1000 individuals); this rate will undoubtedly vary by season and from location to location even within southern province, and data is not currently available to be able to parameterize this value correctly. Because the OpenMalaria simulations assumed an ongoing rate of imported infections, it is not straightforward from model outputs to distinguish between cases arising from imported infections and cases that were a result of local transmission. This parameter is likely a key driver of the differences in the simulated versus observed results of the model validation shown in Fig. 3. If possible, future iterations of field work could improve the ascertainment of mobility and likely imported versus locally transmitted cases. This includes methods such as parasite genotyping or using human movement via mobile phone data as a proxy of parasite flow . Understanding these dynamics will aid not only in developing more relevant baseline model parameterization, but understanding thresholds for importation in a population on the effect of intervention mixes. Combined with transmission estimates, these metrics can provide a more granular view of what intervention mix to deploy, and where.
Demonstrating elimination, and evaluating the ability of interventions to interrupt transmission, requires metrics that are practical in operational settings. The reproductive number under control settings, R c , has been proposed as a metric to determine whether an area has achieved elimination  by calculating the ratio of local cases to imported infections. However, the operational scale at which this should be applied is unclear, as is the geographical definition of infection importation in this context. For example, should importation from higher-transmission zones in the same province be considered as part of the definition, or only importation from a neighbouring country? The area in Southern province currently evaluating anti-malarial administration strategies is bound by the vast Lake Kariba to the east and south, and much lower malaria transmission areas to the north and west, making it an ideal setting to test elimination strategies. A country level metric is likely unsuitable for a country like Zambia which has highly heterogeneous endemicity in different parts of the country. On the other hand, applying such a metric at a more granular administrative level would not confer recognition of elimination by the WHO. Country-specific elimination strategic plans should address how progress will be tracked at both national and sub-national levels.
Other simulation studies and study data suggest the proportion of sub-patent infections missed by RDTs is higher in areas of lower transmission [37, 45, 46], consistent with the simulation results from this study (Fig. 4). Transmission due to asymptomatic infections becomes increasingly important (in relative terms) as cases in a country become fewer and national control programmes approach elimination and prevention of reintroduction. As such, further validation and calibration of models to the prevalence of sub-patent transmissible infections in different epidemiological settings needs to be conducted as new study data becomes available.
Finally, an important limitation for interpreting results is the non-spatially explicit structure of the OpenMalaria model. Focal test and treat and focal MDA strategies that involve targeting specific households, geographic areas or risk groups, and reactive case detection are all implementation strategies in the study area but are not evaluated in the context of this experiment. In addition, there are options for anti-malarial drug administration intervention design that will be relevant in the Southern province that are not explored in this study. Future analyses could examine the potential impact of this larger package of intervention strategies as they are implemented in the same or adjacent geographic areas; such analyses could further contribute to the future programme design by the NMCC.
The optimal strategy for implementation of community-based interventions involving anti-malarial distribution will vary by background level of malaria prevalence and by rate of infections imported to the area. The ability to achieve high coverage of the population is critical to the success of anti-malarial distribution campaigns. This is reflected in the simulation results showing MDA to be more effective than MTAT at reducing mean parasite prevalence 1 year after intervention implementation. While there was a simulated added benefit of the longer prophylactic effect of DHAP compared to AL, the addition of SLD primaquine and ivermectin, both alone and in combination, did not lead to any appreciable reduction in mean parasite prevalence within the year after implementation. Continued coverage and use of optimized vector control interventions is necessary to maximize the gains in reduction of disease burden. OpenMalaria can be helpful in assisting malaria control and elimination programmes to understand what intervention strategies are most appropriate in different settings, and under which conditions these strategies will be the most effective.
EMS designed the experiments, performed the literature review for model parameterization, analysed results and drafted the manuscript. EMS, JMM and ML parameterized the model and contributed to the modelling study design. RS and JMM designed the field studies. JMM supervised and conducted the intervention implementation. RS, EMS and NC conceived and designed the modeling study. All authors participated in designing the experiments and interpreting the results. All authors read and approved the final manuscript.
The authors would like to thank the study participants and communities of Southern province, and the Zambia Ministry of Health and the National Malaria Control Centre at national and province levels, for their support and cooperation. We acknowledge colleagues at the Swiss Tropical and Public Health Institute and PATH MACEPA for helpful discussions. We also acknowledge the MalariaControl.net volunteers for donating computing resources to run simulations.
Funding and ethics statement
This work was supported by the Bill and Melinda Gates Foundation, under grants number OPP1032350 (Swiss TPH) and OPP1089412 (PATH MACEPA).
The experiment design, analysis, and manuscript writing was performed while EMS was employed by the Swiss Tropical and Public Health Institute. At the time of manuscript submission EMS is currently employed by the Bill & Melinda Gates Foundation, who provided funding for both PATH MACEPA and Swiss TPH.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Utzinger J, Tozan Y, Doumani F, Singer BH. The economic payoffs of integrated malaria control in the Zambian copperbelt between 1930 and 1950. Trop Med Int Health. 2002;7:8.View ArticleGoogle Scholar
- Chanda E, Masaninga F, Coleman M, Sikaala C, Katebe C, MacDonald M, et al. Integrated vector management: the Zambian experience. Malar J. 2008;7:164.View ArticlePubMedPubMed CentralGoogle Scholar
- Larsen DA, Bennett A, Hamainza B, Yukich JO, Keating J, Littrell M, et al. Population-wide malaria testing and treatment with rapid diagnostic tests and artemether-lumefantrine in southern Zambia: a community randomized step-wedge control trial design. Am J Trop Med Hyg. 2015;92:913–21.View ArticlePubMedPubMed CentralGoogle Scholar
- Eisele TP, Silumbe K, Finn T, Chalwe V, Kamuliwo M, Hamainza B, et al. Assessing the effectiveness of household-level focal mass drug administration and community-wide mass drug administration for reducing malaria parasite infection prevalence and incidence in southern province, Zambia: study protocol for a community randomized controlled trial. Trials. 2015;16:347.View ArticlePubMedPubMed CentralGoogle Scholar
- Lwin KM, Phyo AP, Tarning J, Hanpithakpong W, Ashley EA, Lee SJ, et al. Randomized, double-blind, placebo-controlled trial of monthly versus bimonthly dihydroartemisinin–piperaquine chemoprevention in adults at high risk of malaria. Antimicrob Agents Chemother. 2012;56:1571–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Sutanto I, Suprijanto S, Kosasih A, Dahlan MS, Syafruddin D, Kusriastuti R, et al. The effect of primaquine on gametocyte development and clearance in the treatment of uncomplicated falciparum malaria with dihydroartemisinin–piperaquine in South sumatra, western Indonesia: an open-label, randomized, controlled trial. Clin Infect Dis. 2013;56:685–93.View ArticlePubMedGoogle Scholar
- Foy BD, Kobylinski KC, da Silva IM, Rasgon JL, Sylla M. Endectocides for malaria control. Trends Parasitol. 2011;27:423–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Steketee RW, ter Kuile F. Single low-dose primaquine to reduce malaria transmission. Lancet Infect Dis. 2014;14:91–2.View ArticlePubMedGoogle Scholar
- WHO. Updated WHO policy recommendation October 2012: single dose primaquine as a gametocytocide in Plasmodium falciparum malaria. Geneva: World Health Organization; 2012.Google Scholar
- Eziefula AC, Bousema T, Yeung S, Kamya M, Owaraganise A, Gabagaya G, et al. Single dose primaquine for clearance of Plasmodium falciparum gametocytes in children with uncomplicated malaria in Uganda: a randomised, controlled, double-blind, dose-ranging trial. Lancet Infect Dis. 2013;14:130–9.View ArticlePubMedGoogle Scholar
- Okebe J, Bousema T, Affara M, DiTanna G, Eziefula AC, Jawara M, et al. The gametocytocidal efficacy of primaquine in malaria asymptomatic carriers treated with dihydroartemisinin–piperaquine in The Gambia (PRINOGAM): study protocol for a randomised controlled trial. Trials. 2015;16:70.View ArticlePubMedPubMed CentralGoogle Scholar
- Determining a tolerable dose of primaquine in G6PD-deficient persons without malaria in Mali (PQSAFETY). ClinicalTrials.gov identifier: NCT02535767 https://clinicaltrials.gov/ct2/show/NCT02535767.
- Sylla M, Kobylinski KC, Gray M, Chapman PL, Sarr MD, Rasgon JL, et al. Mass drug administration of ivermectin in south-eastern Senegal reduces the survivorship of wild-caught, blood fed malaria vectors. Malar J. 2010;9:365.View ArticlePubMedPubMed CentralGoogle Scholar
- Kobylinski KC, Deus KM, Butters MP, Hongyu T, Gray M, da Silva IM, et al. The effect of oral anthelmintics on the survivorship and re-feeding frequency of anthropophilic mosquito disease vectors. Acta Trop. 2010;116:119–26.View ArticlePubMedPubMed CentralGoogle Scholar
- Kobylinski KC, Sylla M, Chapman PL, Sarr MD, Foy BD. Ivermectin mass drug administration to humans disrupts malaria parasite transmission in Senegalese villages. Am J Trop Med Hyg. 2011;85:3–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Chaccour CJ, Kobylinski KC, Bassat Q, Bousema T, Drakeley C, Alonso P, et al. Ivermectin to reduce malaria transmission: a research agenda for a promising new tool for elimination. Malar J. 2013;12:153.View ArticlePubMedPubMed CentralGoogle Scholar
- Penny MA, Maire N, Studer A, Schapira A, Smith TA. What should vaccine developers ask? Simulation of the effectiveness of malaria vaccines. PLoS One. 2008;3:e3193.View ArticlePubMedPubMed CentralGoogle Scholar
- Stuckey EM, Stevenson J, Galactionova K, Baidjoe AY, Bousema T, Odongo W, et al. Modeling the cost effectiveness of malaria control interventions in the highlands of western Kenya. PLoS One. 2014;9:e107700.View ArticlePubMedPubMed CentralGoogle Scholar
- Briet OJ, Hardy D, Smith TA. Importance of factors determining the effective lifetime of a mass, long-lasting, insecticidal net distribution: a sensitivity analysis. Malar J. 2012;11:20.View ArticlePubMedPubMed CentralGoogle Scholar
- OpenMalaria [http://code.google.com/p/openmalaria/wiki/Start]. Accessed 25 Nov 2014.
- Smith T, Maire N, Ross A, Penny M, Chitnis N, Schapira A, et al. Towards a comprehensive simulation model of malaria epidemiology and control. Parasitology. 2008;135:1507–16.View ArticlePubMedGoogle Scholar
- Chitnis N, Hardy D, Smith T. A periodically-forced mathematical model for the seasonal dynamics of malaria in mosquitoes. Bull Math Biol. 2012;74:1098–124.View ArticlePubMedPubMed CentralGoogle Scholar
- Smith T, Ross A, Maire N, Chitnis N, Studer A, Hardy D, et al. Ensemble modeling of the likely public health impact of a pre-erythrocytic malaria vaccine. PLoS Med. 2012;9:e1001157.View ArticlePubMedPubMed CentralGoogle Scholar
- Fornadel CM, Norris LC, Glass GE, Norris DE. Analysis of Anopheles arabiensis blood feeding behavior in southern Zambia during the two years after introduction of insecticide-treated bed nets. Am J Trop Med Hyg. 2010;83:848–53.View ArticlePubMedPubMed CentralGoogle Scholar
- Kent RJ, Thuma PE, Mharakurwa S, Norris DE. Seasonality, blood feeding behavior, and transmission of Plasmodium falciparum by Anopheles arabiensis after an extended drought in southern Zambia. Am J Trop Med Hyg. 2007;76:267–74.PubMedPubMed CentralGoogle Scholar
- ZMOH. Zambia national malaria indicator survey 2010. Lusaka. 2011.Google Scholar
- Chanda P, Hawela M. Assessment of patient compliance to Artemether-Lumefantrine in Zambia. Zambia National Malaria Control Centre ORO ed. 2005.Google Scholar
- Depoortere E, Guthmann JP, Sipilanyambe N, Nkandu E, Fermon F, Balkan S, Legros D. Adherence to the combination of sulphadoxine–pyrimethamine and artesunate in the Maheba refugee settlement, Zambia. Trop Med Int Health. 2004;9:62–7.View ArticlePubMedGoogle Scholar
- PATH/MACEPA. Malaria test and treat campaign summary—NMCC briefing document. Lusaka: PATH/MACEPA; 2013.Google Scholar
- Central Statistical Office ZMoH, Zambia Uo, Centre TDR, Inc. MI. Zambia demographic and health survey. 2009.Google Scholar
- Hamainza BH, Hawela M, Chanda P, Chalwe V, Masaninga F, Kamuliwo M. Efficacy testing for artemether lumefantrine: Mansa site report. Lusaka: National Malaria Control Centre, Ministry of Health; 2011.Google Scholar
- Winter K, Hastings IM. Development, evaluation, and application of an in silico model for antimalarial drug treatment and failure. Antimicrob Agents Chemother. 2011;55:3380–92.View ArticlePubMedPubMed CentralGoogle Scholar
- Kabanywanyi AM, Lengeler C, Kasim P, King’eng’ena S, Schlienger R, Mulure N, et al. Adherence to and acceptability of artemether-lumefantrine as first-line anti-malarial treatment: evidence from a rural community in Tanzania. Malar J. 2010;9:48.View ArticlePubMedPubMed CentralGoogle Scholar
- Fogg C, Bajunirwe F, Piola P, Biraro S, Checchi F, Kiguli J, et al. Adherence to a six-dose regimen of artemether-lumefantrine for treatment of uncomplicated Plasmodium falciparum malaria in Uganda. Am J Trop Med Hyg. 2004;71:525–30.PubMedGoogle Scholar
- Chitnis N, Schapira A, Smith T, Steketee R. Comparing the effectiveness of malaria vector-control interventions through a mathematical model. Am J Trop Med Hyg. 2010;83:230–40.View ArticlePubMedPubMed CentralGoogle Scholar
- Poirot E, Sarbinski J, Sinclair D, Kachur SP, Slutsker L, Hwang J. Mass drug administration for malaria. Cochrane Database Syste Rev. 2013;12:CD008846.Google Scholar
- Tiono AB, Ouedraogo A, Ogutu B, Diarra A, Coulibaly S, Gansané A, et al. A controlled, parallel, cluster-randomized trial of community-wide screening and treatment of asymptomatic carriers of Plasmodium falciparum in Burkina Faso. Malar J. 2013;12:79.View ArticlePubMedPubMed CentralGoogle Scholar
- Halliday KE, Okello G, Turner EL, Njagi K, Mcharo C, Kengo J, et al. Impact of intermittent screening and treatment for malaria among school children in Kenya: a cluster randomised trial. PLoS Med. 2014;11:e1001594.View ArticlePubMedPubMed CentralGoogle Scholar
- Gerardin J, Eckhoff P, Wenger E. Mass campaigns with antimalarial drugs: a modelling comparison of artemether-lumefantrine and DHA-piperaquine with and without primaquine as tools for malaria control and elimination. BMC Infect Dis. 2015;15:144.View ArticlePubMedPubMed CentralGoogle Scholar
- Slater HC, Walker PG, Bousema T, Okell LC, Ghani AC. The potential impact of adding ivermectin to a mass treatment intervention to reduce malaria transmission: a modelling study. J Infect Dis. 2014;210:12.Google Scholar
- Hodel EM, Kay K, Hayes DJ, Terlouw DJ, Hastings IM. Optimizing the programmatic deployment of the anti-malarials artemether-lumefantrine and dihydroartemisinin–piperaquine using pharmacological modelling. Malar J. 2014;13:138.View ArticlePubMedPubMed CentralGoogle Scholar
- Bretscher MT, Griffin J, Hugo P, Baker M, Ghani A, Okell L, et al. A comparison of the duration of post-treatment protection of artemether-lumefantrine, dihydroartemisinin–piperaquine and artesunate-amodiaquine for the treatment of uncomplicated malaria. Malar J. 2014;13(Suppl 1):P19.View ArticlePubMed CentralGoogle Scholar
- Tatem AJ, Huang Z, Narib C, Kumar U, Kandula D, Pindolia DK, et al. Integrating rapid risk mapping and mobile phone call record data for strategic malaria elimination planning. Malar J. 2014;13:52.View ArticlePubMedPubMed CentralGoogle Scholar
- Churcher TS, Cohen JM, Novotny J, Ntshalintshali N, Kunene S, Cauchemez S. Measuring the path toward malaria elimination. Science. 2014;344:1230–2.View ArticlePubMedPubMed CentralGoogle Scholar
- Okell LC, Bousema T, Griffin JT, Ouédraogo AL, Ghani AC, Drakeley CJ. Factors determining the occurrence of submicroscopic malaria infections and their relevance for control. Nat Commun. 2012;3:1237.View ArticlePubMedPubMed CentralGoogle Scholar
- Mosha JF, Sturrock HJ, Greenhouse B, Greenwood B, Sutherland CJ, Gadalla N, et al. Epidemiology of subpatent Plasmodium falciparum infection: implications for detection of hotspots with imperfect diagnostics. Malar J. 2013;12:221.View ArticlePubMedPubMed CentralGoogle Scholar