Effect of dietary intervention on the prevalence of asymptomatic malaria among 6–18-month-old children in rural Malawi
Malaria Journal volume 22, Article number: 266 (2023)
The complex interaction between malaria and undernutrition leads to increased mortality and morbidity rate among young children in malaria-endemic regions. Results from previous interventions suggest that improving nutritional status of young children may reduce the burden of malaria. This study tested a hypothesis that provision of lipid-based nutrient supplements (LNS) or corn-soy blend (CSB) supplementation to 6–18-month-old children in Malawi would reduce the prevalence of asymptomatic malaria among them.
A total of 840 6-month-old children were enrolled in a randomized trial. The participants received 12-month supplementation with three different daily dietary supplementations: CSB, soy-LNS, or milk-LNS, and one control group without supplementation. The prevalence rate of asymptomatic Plasmodium falciparum was determined by real-time PCR from the participant’s dried blood spots (DBS) collected at the baseline and every 3 months. The global null hypothesis was tested using modified Poisson regression to estimate the prevalence ratio (PR) between the control group and three intervention groups at all ages combined. All the models were adjusted for malaria at baseline, season of DBS sample collection, site of enrolment, and household asset Z-score.
All children combined, the prevalence of P. falciparum was 14.1% at enrollment, 8.7% at 9 months, 11.2% at 12 months, 13.0% at 15 months and 22.4% at 18 months of age. Among all samples that were taken after enrolment, the prevalence was 12.1% in control group, 12.2% in milk-LNS, 14.0% in soy-LNS, and 17.2% in CSB group. Compared to children in the control group the prevalence ratio of positive malaria tests was 1.19 (95% CI 0.81–1.74; P = 0.372) in the milk-LNS group, 1.32 (95% CI 0.88–1.96; P = 0.177) in the soy-LNS group and 1.72 (95% CI 1.19–2.49; P = 0.004) in the CSB group.
The study findings do not support a hypothesis that LNS or CSB supplementation would reduce the prevalence of asymptomatic malaria among Malawian children. In contrast, there was a signal of a possible increase in malaria prevalence among children supplemented with CSB.
Malaria is a vector-borne and life-threatening disease estimated to be globally responsible for about 241 million cases and 627 thousand deaths per year . More than 95% of the world’s malaria cases and deaths occur in Africa, and children less than 5 years constitute nearly 80% of deaths . Malaria continues to be a major public health problem in Malawi with 4.4 million infected cases reported in 2018 and 98% of those were caused by Plasmodium falciparum . Malawi represents 2% of global morbidity  and the highest rate of cases occurs in young children .
Asymptomatic malaria infections are poorly understood or have been neglected in research studies and malaria control policies. This insufficient attention may be due to lower parasitaemia intensity without symptoms which makes their detection difficult by microscopy and rapid diagnostic tests (RDTs) [3,4,5]. In malaria-endemic regions, the majority of malaria infections are asymptomatic  particularly among adults and school-age children because of acquired antimalaria immunity . The persistence of asymptomatic malaria-infected carriers in the population is known to stimulate gametocytogenesis , facilitate the recurrence of malaria infection and serve as reservoirs and foci for sustained malaria infections in children, which interrupts malaria elimination [9,10,11,12]. This in turn, affects younger children's health with chronic anaemia and impaired growth that may reduce school performance in later life  and lead to undernutrition which weakens adaptive and innate immunity against parasitic infections and other infectious diseases . Malaria and undernutrition are two leading causes of increased morbidity and mortality rate among children less than 5 years. This complex association persists in an interwoven vicious cycle that coexists in sub-Saharan Africa . The interaction association between undernutrition and reducing asymptomatic malaria endemicity remains inconclusive with inconsistent findings [15,16,17,18,19].
Global health programs recommended micronutrient supplements to vulnerable children under-five years that contain various components of essential vitamins and minerals required for children’s growth to reduce malaria mortality and morbidity [20,21,22]. Nutrient-based interventions may reduce the severity of diseases, accelerate recovery, and stop recurrent infection by improving children’s growth which has a positive effect on the regulation and integrity of the immunity system [23, 24]. Previous studies showed a beneficial effect of lipid-based nutrient supplements (LNS) on the linear growth improvement of infants and young children (6–12 months) [25,26,27,28]. Furthermore, corn-soy blend supplements (CSB) are another common inexpensive composition and the most acceptable and obtainable components used for children with malnutrition in many low-income regions [29, 30].
LNS and CSB dietary interventions display substantial similarity in their nutritional components, featuring slight differentiations in their content. These interventions collectively contribute to the enhancement of children’s linear growth, consequently strengthening the potency of their immune response in the pursuit of disease mitigation. Limited evidence of randomized trials has found a potential protective impact of dietary interventions on the occurrence of malaria in children [17, 31]. Studies suggested that vitamin A and zinc nutritional supplements reduced malaria morbidity in young children [32, 33]. Iron supplements were found to increase the rate of malaria parasitaemia among children . Essential fatty acids derivatives in the lipid base of LNS showed a significant reduction of P. falciparum parasitaemia in an in vitro study . These contradictory impacts of dietary interventions on malaria prevalence left the topic inconclusive. The aim of this study was to test the effect of two LNS and CSB on asymptomatic P. falciparum malaria prevalence among young children in Malawi. The study hypothesis was that the provision of any of the three dietary interventions used for one year would reduce the prevalence rate of asymptomatic P. falciparum among Malawian children (6 to 18 months).
Study design and participants
This study is a secondary analysis of data and biological sample (dried blood spot, DBS) from a clinical trial known as Lungwena Child Nutrition Intervention 5 (LCNI-5). The trial was conducted at and around Lungwena Health Centre and Malindi Hospital catchment area, Mangochi District, Southern Malawi from 2008 to 2009. The LCNI-5 protocol and primary outcome results have been described elsewhere in detail . In brief, a total of 840 6-month-old healthy infants were enrolled and randomized to receive one of three-dietary interventions or no supplements between 6 and 18 months of age: (1) control group without supplements; (2) dietary supplementation with 72 g/day fortified maize-soy flour; (3) dietary supplementation with 54 g/day of milk containing LNS; (4) dietary supplementation with 54 g/day of soy containing LNS (Table 1). Participants were visited every 2 weeks at their homes between 6 and 18 months of age and were provided with a 14-day supply of dietary supplementation.
The trial was performed according to Good Clinical Practice guidelines and ethical standards of the Declaration of Helsinki. The protocol was approved by the College of Medicine Research and Ethics Committee, Malawi, and the Ethical Committee of Pirkanmaa Hospital District, Finland. Only participants whose guardians signed informed consent forms were enrolled in the study. This trial has been registered at www.clinicaltrials.gov (identifier NCT00524446).
Sample collection, DNA extraction, and real-time PCR
At 3-month intervals from enrolment until 18 months old, participants were seen at the health center where they underwent a clinical check-up and blood sampling. From the blood samples taken at the clinic, 100 μl (2 spots, each 50 μl) was applied to Whatman FTA filter paper (Whatman plc, Maidstone, UK), air-dried, and placed in individually sealed plastic bags with a desiccant. The sample bags were then stored in dry condition at room temperature for about 5 years before the analyses. In total, there were 2799 DBS samples stored from this trial in 2008.
The DNA extraction of P. falciparum was carried out according to a slightly modified Chelex-saponin procedure that was previously published . In brief, 6mm of blood spots were punched from each DBS card into a 96-deep-well plate. One ml of 1 × phosphate-buffered saline (PBS) and 50 µl of 10% saponin were added to each well containing DBS. The plate was then covered with a foil plate cover, vortexed for 20–30 s, and left to incubate overnight at 4 °C. After centrifuging and washing twice with 1 ml of PBS, 100 µl of sterile water and 50 µl of 20% Chelex 100-solution were added to each well. The plates were then incubated at 98 °C for 12 min (vortexed every 2–3 min) and thereafter centrifuged at 1500 rpm for 5 min. After that, the supernatant from each well was transferred to a new 96-deep-well plate centrifuged for 10 min at 1500 rpm, covered with foil, labelled, and stored at − 20 °C.
Quantitative polymerase chain reaction (qPCR) was performed at laboratories of the College of Medicine, University of Malawi to amplify the LDH gene, a marker of P. falciparum, in the isolated total genomic DNA. The following published primer and probe sequences were used: Forward primer—ACG ATT TGG CTG GAG CAG AT; reverse primer—TCT CTA TTC CAT TCT TTG TCA CTC TTT C; probe—FAM-AGT AAT AGT AAC AGC TGG ATT TAC CAA GGC CCC A-TAMRA .
All reactions were run in duplicate on the StepOneplus Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA). The cycling conditions were 50 °C for 2 min, 95 °C for 10 min, and 45 cycles of 95 °C for 15 s and then 60 °C for 1 min. Four P. falciparum 3D7 DNA serial dilutions (10, 1, 0.1, 0.01 ng/µl) were used as positive controls along with a non-template control. The threshold cycle (Ct) values were determined for each amplified duplicate after the threshold lines were manually placed for each reaction plate. Once both amplification curves crossed the threshold line, samples were deemed positive. Repeated reactions in the duplicate DNA template occurred when only one amplification curve crossed the threshold line, or the Ct value above 44.
Anthropometric measurements were carried out by three trained research assistants during the enrolment visit. Unclothed infants were weighed using an electronic infant weighing scale (SECA 735; Chasmors Ltd. London, England) and weights were recorded to the nearest 10 g. The length was measured to the nearest 1 mm using a high-quality length board (Kiddimetre; Raven Equipment Ltd, Essex, England). Age- and sex-standardized anthropometric indices (length-for-age Z score [LAZ]), weight-for-age Z score [WAZ] and weight-for-length Z score [WLZ]) were calculated using the STATA macro developed by the World Health Organization (WHO) using the WHO 2006 multicentre growth standards .
With the sample size of 210 participants allocated to each group, the original trial possessed an 85% likelihood of detecting significant contrasts in severe stunting rates. This was achieved while maintaining a two-sided Type I error of 5%, even with a potential 10% drop in participants due to attrition during the follow-up period. Additionally, the exclusion of individuals who were already severely stunted at the outset (as per the initial enrolment criteria) accounted for 5% of the sample. The proportion of positive malaria parasitaemia DBS samples by intervention group at enrollment, at 9, 12, 15, and 18 months was calculated separately, and for all intervention time points combined (9–18 months). All randomized participants were eligible to be included in the analyses, with the exception that subjects with missing data on an outcome variable were excluded from the analysis of that outcome. The modified Poisson regression with a robust variance estimator was used to estimate the prevalence ratio (PR)  between the control group and each three intervention groups at each time point separately, and for all time points combined. For all time points combined, the intragroup correlation due to multiple measurements per participant was taken into account by using robust standard errors for clustered data. The statistical analyses were conducted with Stata 17.0 (StataCorp, College Station, USA).
Null hypothesis of no difference between groups was rejected if P < 0.05. Wald’s test was used to test the global null hypothesis of no differences between the control group and the intervention groups, and the pairwise comparisons. For pairwise comparisons with P < 0.05, the hypothesis of no difference between groups was rejected only if the global null-hypothesis was also rejected.
The main analysis was conducted with covariate adjustments. Covariates used in the adjusted models were derived from a predefined list of variables that could modify the prevalence of malaria parasitaemia. The covariate selection with modified Poisson regression models was performed. All the models were adjusted for the same set of covariates, which were malaria parasitaemia at enrolment, season of DBS sample collection, study site, and household asset Z-score.
A sensitivity analysis was performed by combining children in the soy- and milk-LNS groups and compared it to the control group at any time of study visit to validate the association between LNS and the prevalence of asymptomatic malaria.
Characteristics of the study participants
A total of 1385 infants were screened, out of whom 840 were enrolled in the trial. The recruitment, group allocation, reasons for exclusions at enrollment, and success of their follow-up including available laboratory measurements at different visits are shown in Fig. 1. The participants were randomized into four groups: 209 to the control group, 209 to the CSB group, 212 to the milk-LNS group, and 210 to the soy-LNS group. Infants were not enrolled in the study because they were not brought to an enrollment session, they did not meet the inclusion criteria, or their guardians did not consent to participate in the study (Fig. 1). The proportion of excluded participants was similar in all groups at different visits.
There were no differences among intervention groups in terms of baseline characteristics (Table 2). The proportion of malaria prevalence at enrollment was 14.1%. Approximately 49% of the participating infants were boys. The mean (SD) weight and length were 7.0 (0.9) kg and 63.1 (2.3) cm, respectively. The mean LAZ, WAZ, WLZ were − 1.7 (1.0), − 0.8 (1.1) and 0.5 (1.0), respectively. The mean Hb concentration was 94.7 (16.8) g/L and mean maternal education was 3.5 (3.3) years. More than two thirds (73.2%) of the households used bed nets.
Prevalence of asymptomatic malaria at different time points by intervention groups
Among all the collected blood samples, the proportion of positive malaria tests was 13.9%. After enrollment, the prevalence of asymptomatic malaria was 8.7% at 9 months, 11.2% at 12 months, 13.0% at 15 months, and 22.4% at 18 months of age.
The prevalence of asymptomatic malaria by visit and intervention group is shown in Table 3. There were statistically significant differences in the prevalence of malaria among intervention groups at all ages combined (P = 0.023) and at 15 months of age (P = 0.015). Compared to children in the control group and adjusting for potential confounders, the prevalence ratio of positive malaria test among children in the CSB group was 1.72 (95% CI 1.19–2.49; P = 0.004) at all ages combined and 3.24 (95% CI 1.46–7.17; P = 0.004) at 15 months of age. At 15 months of age, the prevalence ratio of positive malaria test among children in the soy-LNS group was 2.53, 95% CI 1.13–5.66; P = 0.024) compared to those in the control group.
A sensitivity analysis combining children from the soy-LNS and milk-LNS groups into one group confirmed that there was no association between LNS provision and asymptomatic malaria at any of the study visits (see Additional file 1: Appendix Table S1).
The present study evaluated the impact of three different dietary interventions on the occurrence of asymptomatic malaria among 6–18 months old Malawian children. This study reported the overall prevalence of asymptomatic malaria among young children as 14.1% in rural Malawi. There was no statistically significant association found between LNS provision and the prevalence of asymptomatic malaria. In contrary to the original hypotheses, the provision of CSB was associated with a higher malaria prevalence.
Randomized study design, large sample size, investigators blinded to the samples’ status, and use of real-time PCR (sensitive and accurate method for detecting asymptomatic malaria cases) reduced the probability of random error or bias in the current study. Differences in laboratory methods, excluded participants or those with missing data (due to either loss of cases, visits, or blood specimens) could have affected internal validity. Nevertheless, standard procedures for laboratory sample collection, storage, and methods were used across the study. Furthermore, included and excluded participants had similar baseline characteristics. Therefore, the findings of this study are valid and representative and not supportive of a hypothesis that the tested dietary supplements would reduce the prevalence of asymptomatic malaria prevalence in this target population of 6–18-month-old children in Malawi.
There are a limited number of clinical trials that have evaluated the potential effects of micronutrient supplements on children living in malaria-endemic areas which lead to different outcomes either protective, adverse, or no effects [31, 41]. In this study, CSB and LNS interventions have not decreased the prevalence of asymptomatic malaria compared to the control group which did not support the study hypothesis. These might be more related to nutrient composition and the infant’s immune response to asymptomatic malaria. Some of the micronutrient components that were also included in the study daily intervention supplements were proven in previous studies to have a protective impact on the decreased prevalence of malaria including zinc  and no effect was found with thiamine  as a source of nutrients and energy. While iron [44, 45] and folate  have been found to exacerbate malaria morbidity, and riboflavin had an unclear impact .
In this study, CSB supplementation was associated with a higher malaria prevalence among 6–18-month-old children. Among the three intervention used in this study, CSB has a lower nutrient density and it was associated with relatively poor linear growth outcomes in a prior study . Giving a diluted voluminous porridge mixture of CSB to infants might reduce or displace normal breastfeeding and any other home dietary food intake. This in turn might affect innate and adaptive children´s immunity and increase susceptibility to malaria infection [48, 49]. Compared to LNS, CSB supplement has a lower content of retinol (Vitamin A) and zinc with no vitamin D, all of which are considered essential for the normal immune function to modulate malaria morbidity and mortality in different aspects [31, 33, 50, 51]. CSB also lacked the pantothenic acid (vitamin B5) and selenium trace element that both were found in previous studies to have anti-plasmodial activity against P. falciparum [52,53,54]. These explanations are the possible assumptions that need more proof and clarification otherwise considered random findings. Therefore, more research is needed to understand better the exact mechanism for the complex interaction of micronutrients on immunity and malaria.
The study findings did not support the hypothesis that children between 6 to 18 months who receive daily dietary supplementation with LNS or CSB would have a lower risk of asymptomatic malaria in rural Malawi. In contrast, there was a signal of a possible increase in malaria prevalence among children supplemented with CSB. It would be important to monitor malaria parasitaemia with adequate control measures when further studies will be done with micronutrients fortified CSB.
Availability of data and materials
Data described in the manuscript, codebook, and analytic code will be made available upon request pending approval by the authors.
Lipid-based nutrient supplements
Dry blood spot
Lungwena Child Nutrition Intervention 5
Polymerase chain reaction
Lactate dehydrogenase gene
Length-for-age Z score
Weight-for-age Z score
Weight-for-length Z score
World Health Organization
World Health Organization. World malaria report 2021. Geneva: World Health Organization; 2021.
Zgambo M, Mbakaya BC, Kalembo FW. Prevalence and factors associated with malaria parasitaemia in children under the age of five years in Malawi: a comparison study of the 2012 and 2014 Malaria Indicator Surveys (MISs). PLoS ONE. 2017;12: e0175537.
Laishram DD, Sutton PL, Nanda N, Sharma VL, Sobti RC, Carlton JM, et al. The complexities of malaria disease manifestations with a focus on asymptomatic malaria. Malar J. 2012;11:29.
WHO. Test, treat, track: scaling up diagnostic testing, treatment and surveillance for malaria. Geneva, World Health Organization; 2012. Report No.: WHO/HTM/GMP/2012.03. https://apps.who.int/iris/handle/10665/337979
Chen I, Clarke SE, Gosling R, Hamainza B, Killeen G, Magill A, et al. “Asymptomatic” malaria: a chronic and debilitating infection that should be treated. PLoS Med. 2016;13: e1001942.
Lindblade KA, Steinhardt L, Samuels A, Kachur SP, Slutsker L. The silent threat: asymptomatic parasitemia and malaria transmission. Expert Rev Anti Infect Ther. 2013;11:623–39.
Carneiro I, Roca-Feltrer A, Griffin JT, Smith L, Tanner M, Schellenberg JA, et al. Age-patterns of malaria vary with severity, transmission intensity and seasonality in sub-Saharan Africa: a systematic review and pooled analysis. PLoS ONE. 2010;5: e8988.
Bousema JT, Gouagna LC, Drakeley CJ, Meutstege AM, Okech BA, Akim IN, et al. Plasmodium falciparum gametocyte carriage in asymptomatic children in western Kenya. Malar J. 2004;3:18.
Baliraine FN, Afrane YA, Amenya DA, Bonizzoni M, Menge DM, Zhou G, et al. High prevalence of asymptomatic Plasmodium falciparum infections in a highland area of Western Kenya: a cohort study. J Infect Dis. 2009;200:66–74.
Stresman GH, Kamanga A, Moono P, Hamapumbu H, Mharakurwa S, Kobayashi T, et al. A method of active case detection to target reservoirs of asymptomatic malaria and gametocyte carriers in a rural area in Southern Province. Zambia Malar J. 2010;9:265.
Nzobo BJ, Ngasala BE, Kihamia CM. Prevalence of asymptomatic malaria infection and use of different malaria control measures among primary school children in Morogoro Municipality. Tanzania Malar J. 2015;14:491.
Bin Mohanna MA, Bin Ghouth AS, Raja’a YA. Malaria signs and infection rate among asymptomatic schoolchildren in Hajr valley Yemen. East Mediterr Health J. 2007;13:35–40.
Hughes S, Kelly P. Interactions of malnutrition and immune impairment, with specific reference to immunity against parasites. Parasite Immunol. 2006;28:577–88.
Mahapatra A, Mohapatra S, Mitter N, Dash SS, Mishra RP, Satapathy DM. Malaria and malnutrition co-existence among under-five children of tribal dense regions of Odisha: a community based study. Int J Commun Med Public Health. 2018;5:3024–8.
Crookston BT, Alder SC, Boakye I, Merrill RM, Amuasi JH, Porucznik CA, et al. Exploring the relationship between chronic undernutrition and asymptomatic malaria in Ghanaian children. Malar J. 2010;9:39.
Akiyama T, Pongvongsa T, Phrommala S, Taniguchi T, Inamine Y, Takeuchi R, et al. Asymptomatic malaria, growth status, and anaemia among children in Lao People’s Democratic Republic: a cross-sectional study. Malar J. 2016;15:499.
Das D, Grais RF, Okiro EA, Stepniewska K, Mansoor R, van der Kam S, et al. Complex interactions between malaria and malnutrition: a systematic literature review. BMC Med. 2018;16:186.
Donovan CV, McElroy P, Adair L, Pence BW, Oloo AJ, Lal A, et al. Association of malnutrition with subsequent malaria parasitemia among children younger than three years in Kenya: a secondary data analysis of the Asembo Bay Cohort Study. Am J Trop Med Hyg. 2021;104:243–54.
Yadav CP, Hussain SSA, Pasi S, Sharma S, Bharti PK, Rahi M, et al. Linkages between malaria and malnutrition in co-endemic regions of India. BMJ Glob Health. 2023;8: e010781.
Tam E, Keats EC, Rind F, Das JK, Bhutta ZA. Micronutrient supplementation and fortification interventions on health and development outcomes among children under-five in low- and middle-income countries: a systematic review and meta-analysis. Nutrients. 2020;12:289.
Keats EC, Das JK, Salam RA, Lassi ZS, Imdad A, Black RE, et al. Effective interventions to address maternal and child malnutrition: an update of the evidence. Lancet Child Adolescent Health. 2021;5:367–84.
Caulfield LE, Richard SA, Black RE. Undernutrition as an underlying cause of malaria morbidity and mortality in children less than five years old. Am J Trop Med Hyg. 2004;71:55–63.
Gombart AF, Pierre A, Maggini S. A review of micronutrients and the immune system-working in harmony to reduce the risk of infection. Nutrients. 2020;12:236.
Smith TJ, McClung JP. Nutrition, immune function, and infectious disease. Med J. 2021;133–6.
Adu-Afarwuah S, Lartey A, Brown KH, Zlotkin S, Briend A, Dewey KG. Randomized comparison of 3 types of micronutrient supplements for home fortification of complementary foods in Ghana: effects on growth and motor development. Am J Clin Nutr. 2007;86:412–20.
Iannotti LL, Dulience SJL, Green J, Joseph S, François J, Anténor M-L, et al. Linear growth increased in young children in an urban slum of Haiti: a randomized controlled trial of a lipid-based nutrient supplement. Am J Clin Nutr. 2014;99:198–208.
Phuka JC, Maleta K, Thakwalakwa C, Cheung YB, Briend A, Manary MJ, et al. Postintervention growth of Malawian children who received 12-mo dietary complementation with a lipid-based nutrient supplement or maize-soy flour. Am J Clin Nutr. 2009;89:382–90.
Phuka JC, Maleta K, Thakwalakwa C, Cheung YB, Briend A, Manary MJ, et al. Complementary feeding with fortified spread and incidence of severe stunting in 6- to 18-month-old rural Malawians. Arch Pediatr Adolesc Med. 2008;162:619–26.
Marchione TJ. Foods provided through U.S. Government Emergency Food Aid Programs: policies and customs governing their formulation, selection and distribution. J Nutr. 2002;132:2104S-2111S.
de Pee S, Bloem MW. Current and potential role of specially formulated foods and food supplements for preventing malnutrition among 6- to 23-month-old children and for treating moderate malnutrition among 6- to 59-month-old children. Food Nutr Bull. 2009;30:S434-463.
Shankar AH. Nutritional modulation of malaria morbidity and mortality. J Infect Dis. 2000;182:S37-53.
Shankar AH, Genton B, Semba RD, Baisor M, Paino J, Tamja S, et al. Effect of vitamin A supplementation on morbidity due to Plasmodium falciparum in young children in Papua New Guinea: a randomised trial. Lancet. 1999;354:203–9.
Owusu-Agyei S, Newton S, Mahama E, Febir LG, Ali M, Adjei K, et al. Impact of vitamin A with zinc supplementation on malaria morbidity in Ghana. Nutr J. 2013;12:131.
Prasetyani MA, de Mast Q, Afeworki R, Kaisar MMM, Stefanie D, Sartono E, et al. Effect of a short course of iron polymaltose on acquisition of malarial parasitaemia in anaemic Indonesian schoolchildren: a randomized trial. Malar J. 2017;16:50.
Kumaratilake LM, Robinson BS, Ferrante A, Poulos A. Antimalarial properties of n-3 and n-6 polyunsaturated fatty acids: in vitro effects on Plasmodium falciparum and in vivo effects on P. berghei. J Clin Invest. 1992;89:961–7.
Mangani C, Maleta K, Phuka J, Cheung YB, Thakwalakwa C, Dewey K, et al. Effect of complementary feeding with lipid-based nutrient supplements and corn–soy blend on the incidence of stunting and linear growth among 6- to 18-month-old infants and children in rural Malawi. Matern Child Nutr. 2015;11:132–43.
Miguel RB, Coura JR, Samudio F, Suárez-Mutis MC. Evaluation of three different DNA extraction methods from blood samples collected in dried filter paper in Plasmodium subpatent infections from the Amazon region in Brazil. Rev Inst Med Trop Sao Paulo. 2013;55:205–8.
Pickard AL, Wongsrichanalai C, Purfield A, Kamwendo D, Emery K, Zalewski C, et al. Resistance to antimalarials in Southeast Asia and genetic polymorphisms in pfmdr1. Antimicrob Agents Chemother. 2003;47:2418–23.
WHO Multicentre Growth Reference Study Group. Reliability of anthropometric measurements in the WHO Multicentre Growth Reference Study. Acta Paediatr Suppl. 2006;450:38–46.
Zou G. A modified Poisson regression approach to prospective studies with binary data. Am J Epidemiol. 2004;159:702–6.
Osei AK, Hamer DH. Management of Pediatric malaria: role of nutritional interventions. Annales Nestle. 2008;66:31–47.
Shankar AH, Genton B, Baisor M, Paino J, Tamja S, Adiguma T, et al. The influence of zinc supplementation on morbidity due to Plasmodium falciparum: a randomized trial in preschool children in Papua New Guinea. Am J Trop Med Hyg. 2000;62:663–9.
Mayxay M, Khanthavong M, Cox L, Sichanthongthip O, Imwong M, Pongvongsa T, et al. Thiamin supplementation does not reduce the frequency of adverse events after anti-malarial therapy among patients with falciparum malaria in southern Laos. Malar J. 2014;13:275.
Moya-Alvarez V, Cottrell G, Ouédraogo S, Accrombessi M, Massougbodgi A, Cot M. High iron levels are associated with increased malaria risk in infants during the first year of life in Benin. Am J Trop Med Hyg. 2017;97:497–503.
Barffour MA, Schulze KJ, Coles CL, Chileshe J, Kalungwana N, Arguello M, et al. High iron stores in the low malaria season increase malaria risk in the high transmission season in a prospective cohort of rural Zambian children. J Nutr. 2017;147:1531–6.
Das KC, Virdi JS, Herbert V. Survival of the dietarily deprived: folate deficiency protects against malaria in primates. Blood. 1992;80:281a.
Das BS, Das DB, Satpathy RN, Patnaik JK, Bose TK. Riboflavin deficiency and severity of malaria. Eur J Clin Nutr. 1988;42:277–83.
Camacho-Morales A, Caba M, García-Juárez M, Caba-Flores MD, Viveros-Contreras R, Martínez-Valenzuela C. Breastfeeding contributes to physiological immune programming in the newborn. Front Pediatr. 2021;9: 744104.
Stevenson MM, Riley EM. Innate immunity to malaria. Nat Rev Immunol. 2004;4:169–80.
Zeba AN, Sorgho H, Rouamba N, Zongo I, Rouamba J, Guiguemdé RT, et al. Major reduction of malaria morbidity with combined vitamin A and zinc supplementation in young children in Burkina Faso: a randomized double blind trial. Nutr J. 2008;7:7.
Wu B, Du Y, Feng Y, Wang Q, Pang W, Qi Z, et al. Oral administration of vitamin D and importance in prevention of cerebral malaria. Int Immunopharmacol. 2018;64:356–63.
Suradji EW, Hatabu T, Kobayashi K, Yamazaki C, Abdulah R, Nakazawa M, et al. Selenium-induced apoptosis-like cell death in Plasmodium falciparum. Parasitology. 2011;138:1852–62.
Pett HE, Jansen PA, Hermkens PH, Botman PN, Beuckens-Schortinghuis CA, Blaauw RH, et al. Novel pantothenate derivatives for anti-malarial chemotherapy. Malar J. 2015;14:169.
Spry C, Chai CLL, Kirk K, Saliba KJ. A class of pantothenic acid analogs inhibits Plasmodium falciparum pantothenate kinase and represses the proliferation of malaria parasites. Antimicrob Agents Chemother. 2005;49:4649–57.
WHO. Haemoglobin concentrations for the diagnosis of anaemia and assessment of severity. Geneva, World Health Organization; 2011. Report No.: WHO/NMH/NHD/MNM/11.1. https://apps.who.int/iris/handle/10665/85839
We thank the study participants and the staff members of Lungwena Health Centre in Mangochi District for their support and help in the study.
Open access funding provided by Tampere University including Tampere University Hospital, Tampere University of Applied Sciences (TUNI). The LCNI-5 clinical trial was funded by the Academy of Finland (grants 109796 and 127025), with additional funding from the Foundation for Pediatric Research in Finland, the US Agency for International Development under terms of cooperative agreement CHN-A-00-08-00001-00, through the Food and Nutrition Technical Assistance-2 Project managed by the Academy for Educational Development (AED), Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital and the Nutriset Inc. Hany Sady received funding from IIE-Scholar Rescue Fund and EDUFI scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Ethics approval and consent to participate
This clinical trial was performed according to Good Clinical Practice guidelines and the ethical standards of the Declaration of Helsinki. The protocol was approved by the College of Medicine Research and Ethics Committee, Malawi, and the Ethical Committee of Pirkanmaa Hospital District, Finland. Only participants whose guardians signed informed consent forms were enrolled in the study. This trial has been registered at www.clinicaltrials.gov (identifier NCT00524446).
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Sady, H., Chaima, D., Hallamaa, L. et al. Effect of dietary intervention on the prevalence of asymptomatic malaria among 6–18-month-old children in rural Malawi. Malar J 22, 266 (2023). https://doi.org/10.1186/s12936-023-04701-4
- Asymptomatic malaria
- Dietary Intervention
- Prevalence rate