Pharmacokinetics and pharmacodynamics of artesunate and dihydroartemisinin following oral treatment in pregnant women with asymptomatic Plasmodium falciparum infections in Kinshasa DRC
© Onyamboko et al; licensee BioMed Central Ltd. 2011
Received: 29 November 2010
Accepted: 28 February 2011
Published: 28 February 2011
In many malaria-endemic countries, increasing resistance may soon compromise the efficacy of sulphadoxine-pyrimethamine (SP) for intermittent preventative treatment (IPT) of malaria in pregnancy. Artemisinin-based IPT regimens represent a promising potential alternative to SP. Pharmacokinetic and safety data supporting the use of artemisinin derivatives in pregnancy are urgently needed.
Subjects included pregnant women with asymptomatic falciparum parasitaemia between 22-26 weeks (n = 13) or 32-36 weeks gestation (n = 13), the same women at three months postpartum, and 25 non-pregnant parasitaemic controls. All subjects received 200 mg orally administered AS. Plasma total and free levels of AS and its active metabolite DHA were determined using a validated LC-MS method. Non-compartmental pharmacokinetic analysis was performed using standard methods.
All pregnant women delivered live babies. The median birth weight was 3025 grams [range 2130, 3620]; 2 of 26 babies had birth weights less than 2500 grams. Rates of parasite clearance by 12 hours post-dose were high and comparable among the groups. Rapid elimination of AS was observed in all three groups. The 90% CI for the pregnancy:postpartum ratio of geometric means for total and free AUC fell within the pre-specified 0.66 - 1.50 therapeutic equivalence interval. However, more pronounced pharmacokinetic differences were observed between the pregnancy and control subjects, with the 90% CI for the pregnancy:control ratio of geometric means for both total 0.68 (90% CI 0.57-0.81) and free AUC 0.78 (90% CI 0.63-0.95) not fully contained within the 0.66 - 1.50 interval. All subjects cleared parasites rapidly, and there was no difference in the percentage of women who were parasitaemic 12 hours after dosing.
A single dose of orally administered AS was found to be both effective and without adverse effects in this study of second and third trimester pregnant women in the DRC. Although DHA AUC during pregnancy and postpartum were similar, the AUC for the pregnant group was less than the non-pregnant controls. The findings of this study suggest that additional studies on the pharmacokinetics of AS in pregnant women are needed.
Pregnant women are particularly susceptible to malaria, which can lead to intrauterine growth restriction, low birth weight, prematurity, and other poor birth outcomes [1–7]. In order to protect pregnant women against malaria, WHO recommends intermittent preventive treatment (IPT) with sulphadoxine-pyrimethamine (SP) in stable transmission areas. Unfortunately, rates of SP resistance are now quite high in most of the world. For example, Plasmodium falciparum resistance to SP is greater than 57% in the eastern part of the Democratic Republic of Congo (DRC) [8–10]. The growing threat of SP resistance has resulted in substantial concern that SP may become ineffective for IPT in the very near future. Therefore, there is an urgent need to identify efficacious alternative drug regimens [11, 12].
Artemisinin-based combination therapy (ACT) is the current first-line treatment for acute malaria in most malaria-endemic countries . Currently, recommended ACT dosage regimens are based on studies conducted in non-pregnant patients; however, the pharmacokinetics of many drugs is altered during pregnancy. WHO endorses the use of ACT for patients in the second and third trimesters of pregnancy with acute uncomplicated falciparum malaria , but specific dosing recommendations for pregnant women have not been developed due to insufficient pharmacokinetic data in this population. There are also safety concerns regarding the use of artemisinin derivatives in pregnancy, particularly in the first trimester. In response to evidence from animal studies of artemisinin-associated embryotoxicity , WHO guidelines constrain the use of these derivatives in the first trimester to cases of therapeutic failure with alterative agents .
Additional pharmacokinetic and safety information regarding the use of artemisinin derivatives in pregnancy are needed [15–18]. To date, no pharmacokinetic study for an artemisinin derivative has been conducted that included postpartum or non-pregnant controls. Additionally, no such pharmacokinetic study has been conducted with asymptomatic parasitaemic women, the candidate population for IPT. This paper reports the results of a safety and pharmacokinetic study of a single oral dose of artesunate (AS) given to asymptomatic but parasitaemic pregnant women with P. falciparum infection, the same women three months postpartum, and a parallel parasitaemic non-pregnant control group.
The study was conducted at Maternité des Soeurs de Saint Marc de Kingasani, the largest maternity service in Kinshasa. All women from 18 to 40 years of age presenting for antenatal care at less than 22 weeks of gestation assessed on last menstrual period (LMP), were screened for eligibility. Gestational age (GA) was confirmed by ultrasound using the Hadlock method . Women with gestational ages between 8 and 21 weeks (inclusive) were invited to participate in the study. Women were asked during to return during the second trimester of pregnancy (22-26 weeks GA); those not enrolled during the second trimester were screened again during the third trimester (32-36 weeks). Eligibility for the study was established during the women's return visits. Eligibility criteria included the following: no fever (temperature < 37.5°C), infection with P. falciparum (parasite count between 200 to < 300,000 parasites/μL), haematocrit ≥30%, HIV seronegativity, and the absence of other major medical problems (e.g. diabetes, chronic hypertension, etc.).
To determine if women met the eligibility criteria, a complete medical history and physical examination was performed by a study physician; sociodemographic data was collected by a study nurse. Haematocrit was measured using a microhaematocrit centrifuge (Hawksley Haematospin 1400, Hawksley & Sons, Ltd, UK). Thick and thin blood films were stained with Giemsa and then read to determine parasite species and the density of P. falciparum asexual stages. Parasite density, based on examination of at least 200 high-power microscope fields, was calculated as (# trophozoites/#white blood cells)*6000. Human Immunodeficiency Virus (HIV) serology was determined using the Determine 1/2 HIV Test Kit (Inverness Medical Professional Diagnostics, Princeton, NJ). Liver function was assessed by measuring aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in plasmaPiccolo Portable Chemistry Analyser, Abaxis, USA). Alpha-1-Acid glycoprotein was determined in serum samples using radial immunodiffusion (Kent Laboratories, Bellingham, WA, USA).
There were two groups of non-pregnant controls. One group of controls (n = 26) was comprised of the pregnant study subjects at their three month postpartum visits. The second group of controls (n = 25) consisted of malaria-infected, asymptomatic non-pregnant women. Women were recruited from the area around the hospital and from one suburban health center. Interested women were directed to the research center where they were tested to assess eligibility using the same criteria as the pregnant cases.
In order to confirm malaria infection, DNA was later extracted from dried blood spots and analysed by real time PCR using pan-species primers and probe .
Ethical clearance and consent
The study protocol was approved by the ethics committees of Kinshasa School of Public Health, the University of North Carolina at Chapel Hill, and RTI International. The study was first introduced to the pregnant women during their first antenatal clinic (ANC) visit in order to obtain consent for early ultrasound screening. Consent for laboratory screening was obtained from these women when they returned during their second or third trimester of pregnancy, and from the non-pregnant controls at the time of enrollment. Consent for the two days inpatient was obtained from all subjects after determining eligibility.
Treatment and sampling
Study subjects received an oral dose of 200 mg AS as four 50 mg tablets (manufactured by Guilin Pharmaceutical Co. Ltd, Guangxi, Republic of China) after a four-hour fasting period. The four tablets were given orally with 240 mL water under medical supervision. A catheter was inserted into a forearm vein for pharmacokinetic sample collection and flushed after each blood draw with saline solution. Blood (5 ml) was drawn from the IV catheter within a 30 minute period before drug intake and then at the following times after AS intake: 0.25, 0.5, 0.75, 1, 1.5 2, 3, 4, 6, and 8 hours. Sodium fluoride/potassium oxalate was used as an anticoagulant. Blood for malaria smears were taken by finger prick at pre-dose and at 12, 24, 30, 36, 42 and 48 hours post-dose. Malaria smears were also performed on days 7, 14 and 28.
Blood samples were centrifuged immediately at -4°C (1,100 × g/or 2600 rpm, 10 min; Eppendorf refrigerated centrifuge) and the plasma initially stored in liquid nitrogen before being transferred to a -80°C freezer. Twenty-four hours after receiving AS, malaria-infected women received 1725 mg SP to complete malaria treatment in accordance with National Malaria Control Program guidelines. The persistence of malaria infection and adverse events were tracked for 28 days after SP administration. Babies born from the pregnant women cohort were followed until one year of age and development assessed.
Plasma concentrations of AS and its active metabolite dihydroartemisinin (DHA) were determined using a validated liquid chromatography-mass spectrometric method described by Naik et al with slight modifications. Briefly, AS, DHA and the internal standard artemisinin were extracted from 0.25 mL of human plasma using solid phase extraction. The reconstituted extracts were chromatographed isocratically and the compounds detected and quantified by mass spectroscopy. The lower limit of quantification for both AS and DHA was 1 ng/mL. Unbound DHA was assayed using ultrafiltration.
Pharmacokinetic and statistical analysis
Plasma AS and DHA concentration data were analyzed by noncompartmental methods using the program WinNonlin, version 5.2 (Pharsight, Mountain View, CA). Specifically, Cmax and Tmax were taken from observed concentrations and the terminal slope, λz, determined for DHA by non-linear regression of the terminal portion of the concentration vs. time profile using a 1/Y weighting. The DHA elimination half-life was calculated as 0.693/λz. The area under the DHA concentration-time curve (AUCtotal) was determined using the trapezoidal method and total DHA concentrations, with extrapolation of the AUC after the final concentration estimated as Clast/λz. The apparent plasma clearance (CL/F) was determined as Dose/AUCtotal and apparent volume of distribution (Vd/F) calculated as Dose/(λz * AUCtotal) where the AS dose was assumed to be totally and exclusively converted to DHA. Thus the DHA dose was assumed to be equal to the AS dose on a molar basis (DHA dose = AS dose * (284.35/384.42)), the latter ratio representing the molecular weights of DHA and AS. The DHA free fraction (FF) was determined as the ratio of unbound/total drug concentration at each time point where both measurements were collected, with mean FF calculated for each participant within a pharmacokinetic visit. The area under the unbound DHA concentration-time curve (AUCfree) was calculated as FF*AUCtotal. The AUC of AS was also determined using the trapezoidal method. Concentrations below the lower limit of quantification were assigned a value of zero. The terminal slope of the AS concentration profile could not be estimated due to the limited samples after Cmax with concentrations above the lower limit of quantification.
The primary pharmacokinetic outcome measured to assess dosing adequacy was DHA AUCfree. The AUCs for unbound DHA during pregnancy and postpartum were compared using each subject as her own control. The ratio of the AUC during pregnancy to the AUC at postpartum (after log transformation) was considered a measure of the impact of pregnancy on systemic exposure. The interval between population ratios of 0.66 and 1.5 was defined as an interval of "no effect" of pregnancy on pharmacokinetics. The "no effect" interval was selected based on the dose-ranging study by Angus and coworkers . Their sigmoid Emax pharmacodynamic model suggested that the dose with 50% maximal effect on parasite clearance time (PCT) was 1.46 mg/kg or ~100 mg, with a sharp increase in response around this value (Hill slope coefficient of 10). Based on these data and available formulation strengths, we chose an exposure of 66% for non-pregnant women (equivalent to 132 mg) as the lower bound of our no-effect window. If the 90% confidence interval (CI) of pregnancy/postpartum DHA AUCfree was determined to be within the 0.66 and 1.5 interval, then the DHA exposure was deemed as clinically equivalent between pregnancy and postpartum. Statistical analyses were performed using SAS/STAT software, version 9.2 (SAS Institute, Inc., Cary, NC, 2008). For comparisons involving non-compartmental parameters, p-values provided with geometric mean estimates and 90% CIs were based on (paired or 2-sample) t-tests (on logged data, except as noted) and with medians were based on (signed rank or rank sum) Wilcoxon tests. Spearman correlations were used to look for associations among the pregnant and control population. All p-values were 2-sided.
Demographic characteristics on admission of women with Plasmodium falciparum infection (pregnant cases and non-pregnant controls)
(22-26 wks GA)
(32-36 wks GA)
21.7 ± 2.7
26.6 ± 4.4
24.2 ± 4.4
24.8 ± 5.5
1.6 ± 0.1
1.6 ± 0.1
1.6 ± 0.1
1.6 ± 0.1
55.5 ± 8.3
63.0 ± 7.2
55.2 ± 10.6
Postpartum (3 months) weight (kg)‡
53.7 ± 7.6
58.0 ± 4.3
55.8 ± 6.4
21.2 ± 2.8
24.4 ± 2.2
22.7 ± 3.0
21.0 ± 3.2
Postpartum (3 months) BMI (kg/m2)‡
20.3 ± 2.3
22.6 ± 1.7
21.4 ± 2.3
9.0 ± 3.6
9.5 ± 3.0
9.2 ± 3.3
9.8 ± 3.8
0 (0 - 2)
1 (0 - 4)
1 (0 - 4)
0 (0 - 4)
GA at ultrasound screening (weeks)‡
18.9 ± 1.5
18.5 ± 1.5
GA at study screening (weeks)‡
22.4 ± 0.8
33.2 ± 1.4
Body temperature (°C)║
(36.1 - 36.7)
(36.2 - 36.5)
(36.1 - 36.6)
(36.4 - 36.8)
(30.0 - 30.0)
(31.0 - 33.0)
(30.0 - 33.0)
(34.0 - 38.0)
Dosage (mg/kg) ║
Pharmacodynamics of AS in malaria-infected women
(22-26 wks GA)
(32-36 wks GA)
Parasitaemic - n (%)
Density (where > 0)†
(264 - 842)
(404 - 659)
(372 - 842)
(325 - 2215)
0 hours (AS dosing)
Parasitaemic - n (%)
Density (where > 0)†
(179 - 857)
(130 - 788)
(124 - 957)
Parasitaemic - n (%)
Density (where > 0)†
(44 - 286)
(44 - 286)
Parasitaemia was confirmed in all subjects by PCR at the time of recruitment. However, two pregnant women and 11 non-pregnant controls were microscopically aparasitaemic by the time the pharmacokinetic experiment started, usually a day later. Of the 24 pregnant women who were parasitaemic at t = 0, 21 (87.5%) were aparasitaemic at 12 hrs (Table 2). Similarly, 13 of the 14 (93%) non-pregnant controls with parasites at t = 0 were aparasitaemic at 12 hours. One pregnant woman was parasitaemic on day 28. Two others had gametocytes on day 7, one of whom also had gametocytes on day 14. Two other women from the pregnant group were parasitaemic at three months postpartum; one of these had a mixed infection with (P. falciparum and P. malariae). There were no significant differences between pregnant cases and controls in the percentage of women who were non-parasitaemic at 12 hours (Table 2).
In all three groups, AS was rapidly hydrolyzed to DHA, with AS concentrations below the lower limit of quantification (1 ng/mL) after 4 hours in all but two samples. The median AS AUCs were 205, 132 and 187 ng*h/mL for pregnancy, postpartum and non-pregnant women, respectively, with AUCs ranging among the subjects by more than 17-fold.
DHA noncompartmental pharmacokinetic data in pregnant, postpartum, and non-pregnant women with Plasmodium falciparum infection.
Ante vs. Post*
Ante vs. Non-pregnant controls†
Post vs. Non-pregnant controls†
[363.9 - 2541.9]
[414.5 - 2246.3]
[539.8 - 3259.4]
[0.25 - 6.00]
[0.50 - 4.00]
[0.50 - 4.03]
[1034.7 - 4085.5]
[1308.9 - 4054.9]
[1244.8 - 6363.5]
[138.7 - 663.9]
[186.5 - 708.3]
[197.9 - 1301.3]
[36.2 - 142.9]
[36.5 - 113.0]
[23.2 - 118.8]
[0.75 - 2.46]
[0.63 - 2.32]
[0.53 - 1.89]
[0.83 - 2.71]
[0.92 - 2.18]
[0.86 - 6.55]
[59.9 - 335.9]
[66.0 - 332.7]
[38.5 - 400.1]
[1.25 - 5.33]
[1.55 - 5.37]
[0.78 - 6.56]
[0.13 - 0.31]
[0.11 - 0.27]
[0.09 - 0.39]
DHA noncompartmental pharmacokinetic data in pregnant, postpartum, and non-pregnant women with Plasmodium falciparum infection.
Antepartum vs. Postpartum*
Antepartum vs. Non-pregnant controls†
Postpartum vs. Non-pregnant controls†
1.06 (0.87, 1.30)
0.81 (0.63, 1.03)
0.77 (0.61, 0.98)
0.99 (0.74, 1.33)
0.90 (0.66, 1.23)
0.89 (0.67, 1.17)
0.87 (0.77, 0.98)
0.68 (0.57, 0.81)
0.78 (0.66, 0.92)
0.99 (0.85, 1.15)
0.78 (0.63, 0.95)
0.78 (0.65, 0.95)
1.21 (1.09, 1.33)‡
1.48 (1.24, 1.76)
1.29 (1.09, 1.52)
1.16 (1.03, 1.29)‡
1.36 (1.18, 1.57)
1.24 (1.07, 1.44)
0.84 (0.75, 0.96)
0.86 (0.72, 1.02)
1.03 (0.86, 1.22)
0.97 (0.83, 1.14)
1.26 (1.00, 1.60)
1.32 (1.04, 1.67)
0.95 (0.81, 1.12)
1.18 (0.96, 1.44)
1.26 (1.02, 1.56)
1.14 (1.05, 1.24)
1.15 (1.02, 1.28)
1.01 (0.89, 1.14)
The median DHA AUC was significantly higher in the non-pregnant control group than in the pregnant group (2787 vs 1731 ng*h/mL, respectively p = 0.0018; estimated ratio of geometric means, 0.68, 90% CI [0.57, 0.81], p = 0.0004); the difference in Cmax did not reach statistical significance (1285 vs 905 ng*h/mL respectively, p = 0.24; 0.81 [0.63, 1.03], p = 0.15). The estimated ratio of geometric means for free DHA AUC in the pregnancy and control groups was 0.78, 90% CI [0.63, 0.95], p = 0.039. The DHA free fraction was significantly higher in the pregnancy group than control groups but the free DHA AUC remained higher in non-pregnant than the pregnancy group.
All pregnancies were followed until delivery and resulted in singleton live births. There were no congenital abnormalities. The median birth weight was 3025 grams [range 2130, 3620] with 7.7% (2/26) weighing ≤ 2500 grams. Twenty-three of the 26 babies were followed until one year of age and at that time had normal physical and neurological development.
There were no adverse events observed in any subjects. Statistically significant increases in some laboratory parameters were observed (Additional file 1), but the magnitude of these changes was small. In some subjects, transient elevations of ALT and AST above the upper limits of normal were observed. However, as these elevations were less than twice the upper limits of normal, they were considered clinically insignificant.
The study was conducted in the DRC, where high in vivo resistance to SP [8–10] could soon compromise the efficacy of SP for IPT in pregnancy. An IPT regimen incorporating an artemisinin derivative, such as AS, could provide a promising alternative to SP. However, despite the broad implementation of ACTs globally for treatment of acute falciparum malaria, questions remain regarding the safety, pharmacokinetics, and efficacy of artemisinin derivatives in pregnant women. The present study was designed to assess the safety and pharmacokinetics of AS in a population of asymptomatic parasitaemic women in the second and third trimesters of pregnancy, compared to the same women at three months postpartum and a control group of parasitaemic non-pregnant controls.
The pharmacokinetic analysis in this study indicates that in all three groups of women, AS was rapidly hydrolysed to its active metabolite DHA, becoming undetectable within 4 hours. This observed rapid AS disappearance is consistent with results previously reported in the literature [23, 24]. This rapid disappearance precluded a comprehensive characterization of AS pharmacokinetics. However, given that DHA is the principle source of antimalarial activity following AS administration, the central focus of this pharmacokinetic analysis was the assessment of DHA pharmacokinetic alterations occurring during pregnancy. Pregnancy has been associated with substantial changes in the absorption, distribution, metabolism and excretion of a variety of drugs. High levels of progesterone may affect drug absorption. Increased plasma volume and altered drug binding protein concentrations can result in an increased volume of distribution. Pregnancy may increase metabolism of drugs, possibly through increased hepatic blood flow and increased free-fraction of drugs [25, 26]. Decreased plasma concentrations of therapeutic drugs in pregnancy can be a result of increased volume of distribution, increased pre-systemic clearance, increased metabolism or excretion, decreased protein binding, or any combination of these factors. Given the importance of maintaining therapeutic anti-malarial blood levels, reductions in DHA blood levels resulting from such pregnancy-induced pharmacokinetic changes could potentially worsen clinical outcomes.
In this study, pregnant women had significantly lower plasma concentrations (AUC) of DHA s compared to non-pregnant controls. The 90% CI for DHA Cmax, AUCall and AUCfree ratios in pregnancy compared to three months postpartum fell within the protocol specified 0.66-1.50 interval for the ratio. However, the median total and free AUCs for DHA in pregnant women were only 62% and 79% of the median total and free AUC for DHA in the non-pregnant controls. This decreased DHA exposure in pregnant women relative to non-pregnant controls is consistent with increased CL/F and possibly increased Vd/F during pregnancy.
There are several possible explanations for the apparent paradox of the similarity between DHA levels in pregnant women compared to levels in these women at three months postpartum but reduced levels compared to non-pregnant women controls. First, there are a variety of physiological and genetic factors that affect drug absorption, distribution and metabolism. A comparison of pharmacokinetic parameters in the same women during and after pregnancy mitigates the effect of most these potential confounders. Second, it is possible that many of the physiological changes that occur during pregnancy remain three months postpartum and continue to differentiate postpartum women from non-pregnant women. Only a few of the postpartum women were parasitaemic at the time of their repeat evaluation, which may also impact DHA pharmacokinetics. Finally, all women were lactating at three months post-partum; it is possible that lactation effects drug disposition.
Although the 90% confidence intervals for the DHA pharmacokinetic parameter ratios for the pregnant and postpartum subjects were within the 0.66-1.50 equivalence interval, the median values of AUCall and CL/F, but not Vd/F, in the postpartum subjects were trending toward those in the non-pregnant parallel-group subjects. Therefore, if the non-pregnant control group pharmacokinetic parameter estimates are representative of the pre-pregnant pharmacokinetics of the pregnant/post-partum group, then the return of pharmacokinetic parameters to presumed baseline may not have occurred completely at three months postpartum.
There are few previous pharmacokinetic studies in which postpartum subjects were compared intrasubject to the pregnant state. In a study of caffeine during and after pregnancy, the pharmacokinetics returned to predicted values in non-pregnant women within 4 days postpartum . In another study, the estimated mean midazolam pharmacokinetic parameters, which reflected significantly decreased AUC and Cmax corresponding with increased apparent clearance during pregnancy, had returned to apparent normal in the postpartum period (weeks 6-10). In the same study, digoxin (p-glycoprotein substrate) pharmacokinetic parameters had also returned to normal by the post-partum period . A multi-site, multi-country study on the pharmacokinetics of sulphadoxine and pyrimethamine during pregnancy and the postpartum period showed variable results by site and an inconsistency with pharmacokinetic parameters previously reported in the literature . Lactation state has also been shown to modify alcohol pharmacokinetics in women . Based on these examples, the time course to return to apparent "baseline" may, among other explanations, be chemotype-specific, lactation-state dependent, or pharmacogenomically-influenced. That there were inconsistencies reported for sulfadoxine-pyrimethamine both within- and between studies suggests that caution should be exercised in making dosing decisions based on a single study.
The presence of parasitaemia could potentially be contributing to the difference between the postpartum women and the non-pregnant controls, given that malaria infection itself may lead to altered pharmacokinetics . Although all subjects in the present study, including postpartum subjects, were PCR-confirmed parasitaemic at screening prior to treatment with AS, only two of the postpartum women were parasitaemic by microscopy of Giemsa-stained blood films at the time of their pharmacokinetic assessments. Whether differences in parasitaemia influenced differences in pharmacokinetic parameter estimates is unknown. However, subjects were asymptomatic with low levels of parasites, which suggests that the influence of parasitaemia on pharmacokinetic parameter estimates may be minimal.
A prior study conducted by McGready et al in Thailand suggested that pregnancy was associated with decreased exposure to DHA, and increased DHA CL/F and Vd/F, as compared to results obtained in previous studies with non-pregnant adults . This study evaluated the pharmacokinetics of DHA following AS administration using samples from the final day of a three day course of treatment for acute uncomplicated falciparum malaria in second and third trimester pregnant women. Their reported median value for DHA CL/F is higher than the values determined here, as well as higher than previously reported by Newton et al in non-pregnant patients with falciparum malaria during the acute or convalescent phase of infection . The median DHA Vd/F observed in the McGready study (3.4 L/kg; 90% range 0.9-60.7) was also somewhat higher than the value observed in our analysis. This difference could, in part, be due to the fact that the women in the McGready study were given a different dosage of artesunate (4 mg/kg) in conjunction with Malarone®. However, the substantially greater degree of variability in DHA CL/F and Vd/F observed in the McGready study as compared to our study, as well as a lack of a postpartum or non-pregnant control group, complicates a direct comparison of findings.
Both the pregnant women and non-pregnant controls in our study displayed rapid clearance of parasitaemia following administration of the 200 mg oral dose of AS. Differences in parasite clearance were not detected between the groups, despite the lower DHA AUC of the pregnant women. However, the possibility that a higher dose of AS in pregnancy, especially in larger women, would enhance the parasite clearance cannot be excluded.
No apparent AS toxicity to either mother or child was observed in this study. Concerns about the safety of artemisinin derivatives in pregnancy indicate that toxicity may be confined to the first trimester, with studies in rodents and primates suggesting that the artemisinin derivatives may be embryotoxic . The positive safety findings of this study provide additional evidence that any toxicity risk of first trimester use may not extend into later periods of gestation.
Oral AS was found to be effective and without adverse effects in this study of Congolese pregnant women. No clinically relevant differences were seen in DHA pharmacokinetics between treated pregnant women and the same women three months postpartum. In contrast, the pregnant women had much lower AUCs than non-pregnant controls. These results suggest that further studies on the pharmacokinetics of AS in pregnant women are needed.
We thank the staff of Maternité des Soeurs de Saint Marc de Kingasani, the community health workers of the Kingasani Health Zone and their MCZ, and the dedicated research staff from the Kinshasa School of Public Health for all their support and commitment in this research project. We thank RJ Nemeyer for the PCR analyses. We have a special thanks addressed to all the participants (pregnant and non-pregnant women) for their volunteered participation in our study.
A Global Network Study supported by grants from NICHD
- World Health Organization: World Malaria Report 2009. 2009, Geneva, Switzerland: World Health OrganizationView ArticleGoogle Scholar
- Shulman CE, Dorman EK, Bulmer JN: Malaria as a cause of severe anaemia in pregnancy. Lancet. 2002, 360: 494-10.1016/S0140-6736(02)09662-9.View ArticlePubMedGoogle Scholar
- Menendez C, Ordi J, Ismail MR, Ventura PJ, Aponte JJ, Kahigwa E, Font F, Alonso PL: The impact of placental malaria on gestational age and birth weight. J Infect Dis. 2000, 181: 1740-1745. 10.1086/315449.View ArticlePubMedGoogle Scholar
- Brabin B, Prinsen-Geerligs P, Verhoeff F, Kazembe P: Anaemia prevention for reduction of mortality in mothers and children. Trans R Soc Trop Med Hyg. 2003, 97: 36-38. 10.1016/S0035-9203(03)90014-9.View ArticlePubMedGoogle Scholar
- Steketee RW, Nahlen BL, Parise ME, Menendez C: The burden of malaria in pregnancy in malaria-endemic areas. Am J Trop Med Hyg. 2001, 64 (1-2 Suppl): 28-35.PubMedGoogle Scholar
- Menendez C, D'Alessandro U, ter Kuile FO: Reducing the burden of malaria in pregnancy by preventive strategies. Lancet Infect Dis. 2007, 7: 126-135. 10.1016/S1473-3099(07)70024-5.View ArticlePubMedGoogle Scholar
- World Health Organization: A strategic framework for malaria prevention and control during pregnancy in the African region. 2004, Brazzaville: WHO Regional Office for AfricaGoogle Scholar
- Alker AP, Kazadi WM, Kutelemeni AK, Bloland PB, Tshefu AK, Meshnick SR: dhfr and dhps genotype and sulfadoxine-pyrimethamine treatment failure in children with falciparum malaria in the Democratic Republic of Congo. Trop Med Int Health. 2008, 13: 1384-1391. 10.1111/j.1365-3156.2008.02150.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Swarthout TD, van den Broek IV, Kayembe G, Montgomery J, Pota H, Roper C: Artesunate + amodiaquine and artesunate + sulphadoxine-pyrimethamine for treatment of uncomplicated malaria in Democratic Republic of Congo: a clinical trial with determination of sulphadoxine and pyrimethamine-resistant haplotypes. Trop Med Int Health. 2006, 11: 1503-1511. 10.1111/j.1365-3156.2006.01710.x.View ArticlePubMedGoogle Scholar
- Cohuet S, Bonnet M, Van Herp M, Van Overmeir C, D'Alessandro U, Guthmann JP: Short report: molecular markers associated with Plasmodium falciparum resistance to sulfadoxine-pyrimethamine in the Democratic Republic of Congo. Am J Trop Med Hyg. 2006, 75: 152-154.PubMedGoogle Scholar
- Sevene E, Gonzalez R, Menendez C: Current knowledge and challenges of antimalarial drugs for treatment and prevention in pregnancy. Expert Opin Pharmacother. 2010, 11: 1277-1293. 10.1517/14656561003733599.View ArticlePubMedGoogle Scholar
- Ward SA, Sevene EJ, Hastings IM, Nosten F, McGready R: Antimalarial drugs and pregnancy: safety, pharmacokinetics, and pharmacovigilance. Lancet Infect Dis. 2007, 7: 136-144. 10.1016/S1473-3099(07)70025-7.View ArticlePubMedGoogle Scholar
- World Health Organization: Guidelines for the treatment of malaria. 2010, Geneva, Switzerland: World Health Organization, 2Google Scholar
- Clark RL: Embryotoxicity of the artemisinin antimalarials and potential consequences for use in women in the first trimester. Reprod Toxicol. 2009, 28: 285-296. 10.1016/j.reprotox.2009.05.002.View ArticlePubMedGoogle Scholar
- McGready R, Stepniewska K, Lindegardh N, Ashley EA, La Y, Singhasivanon P, White NJ, Nosten F: The pharmacokinetics of artemether and lumefantrine in pregnant women with uncomplicated falciparum malaria. Eur J Clin Pharmacol. 2006, 62: 1021-1031. 10.1007/s00228-006-0199-7.View ArticlePubMedGoogle Scholar
- McGready R, Stepniewska K, Ward SA, Cho T, Gilveray G, Looareesuwan S, White NJ, Nosten F: Pharmacokinetics of dihydroartemisinin following oral artesunate treatment of pregnant women with acute uncomplicated falciparum malaria. Eur J Clin Pharmacol. 2006, 62: 367-371. 10.1007/s00228-006-0118-y.View ArticlePubMedGoogle Scholar
- Rijken MJ, McGready R, Boel ME, Barends M, Proux S, Pimanpanarak M, Singhasivanon P, Nosten F: Dihydroartemisinin-piperaquine rescue treatment of multidrug-resistant Plasmodium falciparum malaria in pregnancy: a preliminary report. Am J Trop Med Hyg. 2008, 78: 543-545.PubMedGoogle Scholar
- Meshnick SR: Artemisinin: mechanisms of action, resistance and toxicity. Int J Parasitol. 2002, 32: 1655-1660. 10.1016/S0020-7519(02)00194-7.View ArticlePubMedGoogle Scholar
- Hadlock FP, Deter RL, Carpenter RJ, Park SK: Estimating fetal age: effect of head shape on BPD. AJR Am J Roentgenol. 1981, 137: 83-85.View ArticlePubMedGoogle Scholar
- Taylor SM, Juliano JJ, Trottman PA, Griffin JB, Landis SH, Kitsa P, Tshefu AK, Meshnick SR: High-throughput pooling and real-time PCR-based strategy for malaria detection. J Clin Microbiol. 2010, 48: 512-519. 10.1128/JCM.01800-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Naik H, Murry DJ, Kirsch LE, Fleckenstein L: Development and validation of a high-performance liquid chromatography-mass spectroscopy assay for determination of artesunate and dihydroartemisinin in human plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2005, 816: 233-242. 10.1016/j.jchromb.2004.11.042.View ArticlePubMedGoogle Scholar
- Angus BJ, Thaiaporn I, Chanthapadith K, Suputtamongkol Y, White NJ: Oral artesunate dose-response relationship in acute falciparum malaria. Antimicrob Agents Chemother. 2002, 46: 778-782. 10.1128/AAC.46.3.778-782.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Binh TQ, Ilett KF, Batty KT, Davis TM, Hung NC, Powell SM, Thu LT, Thien HV, Phuong HL, Phuong VD: Oral bioavailability of dihydroartemisinin in Vietnamese volunteers and in patients with falciparum malaria. Br J Clin Pharmacol. 2001, 51: 541-546. 10.1046/j.1365-2125.2001.01395.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Batty KT, Thu LT, Davis TM, Ilett KF, Mai TX, Hung NC, Tien NP, Powell SM, Thien HV, Binh TQ: A pharmacokinetic and pharmacodynamic study of intravenous vs oral artesunate in uncomplicated falciparum malaria. Br J Clin Pharmacol. 1998, 45: 123-129. 10.1046/j.1365-2125.1998.00655.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Frederiksen MC: Physiologic changes in pregnancy and their effect on drug disposition. Semin Perinatol. 2001, 25: 120-123. 10.1053/sper.2001.24565.View ArticlePubMedGoogle Scholar
- Soldin OP, Mattison DR: Sex differences in pharmacokinetics and pharmacodynamics. Clin Pharmacokinet. 2009, 48: 143-157. 10.2165/00003088-200948030-00001.PubMed CentralView ArticlePubMedGoogle Scholar
- Brazier JL, Ritter J, Berland M, Khenfer D, Faucon G: Pharmacokinetics of caffeine during and after pregnancy. Dev Pharmacol Ther. 1983, 6: 315-322.PubMedGoogle Scholar
- Hebert MF, Easterling TR, Kirby B, Carr DB, Buchanan ML, Rutherford T, Thummel KE, Fishbein DP, Unadkat JD: Effects of pregnancy on CYP3A and P-glycoprotein activities as measured by disposition of midazolam and digoxin: a University of Washington specialized center of research study. Clin Pharmacol Ther. 2008, 84: 248-253. 10.1038/clpt.2008.1.View ArticlePubMedGoogle Scholar
- Nyunt MM, Adam I, Kayentao K, van Dijk J, Thuma P, Mauff K, Little F, Cassam Y, Guirou E, Traore B, Doumbo O, Sullivan D, Smith P, Barnes KI: Pharmacokinetics of sulfadoxine and pyrimethamine in intermittent preventive treatment of malaria in pregnancy. Clin Pharmacol Ther. 2010, 87: 226-234. 10.1038/clpt.2009.177.View ArticlePubMedGoogle Scholar
- Pepino MY, Steinmeyer AL, Mennella JA: Lactational state modifies alcohol pharmacokinetics in women. Alcohol Clin Exp Res. 2007, 31: 909-918. 10.1111/j.1530-0277.2007.00387.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Teja-Isavadharm P, Watt G, Eamsila C, Jongsakul K, Li Q, Keeratithakul G, Sirisopana N, Luesutthiviboon L, Brewer TG, Kyle DE: Comparative pharmacokinetics and effect kinetics of orally administered artesunate in healthy volunteers and patients with uncomplicated falciparum malaria. Am J Trop Med Hyg. 2001, 65: 717-721.PubMedGoogle Scholar
- Newton P, Suputtamongkol Y, Teja-Isavadharm P, Pukrittayakamee S, Navaratnam V, Bates I, White N: Antimalarial bioavailability and disposition of artesunate in acute falciparum malaria. Antimicrob Agents Chemother. 2000, 44: 972-977. 10.1128/AAC.44.4.972-977.2000.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.