Resistance to malaria in humans: the impact of strong, recent selection
© Hedrick; licensee BioMed Central Ltd. 2012
Received: 25 September 2012
Accepted: 18 October 2012
Published: 22 October 2012
Malaria is one of the leading causes of death worldwide and has been suggested as the most potent type of selection in humans in recent millennia. As a result, genes involved in malaria resistance are excellent examples of recent, strong selection. In 1949, Haldane initially suggested that infectious disease could be a strong selective force in human populations. Evidence for the strong selective effect of malaria resistance includes the high frequency of a number of detrimental genetic diseases caused by the pleiotropic effects of these malaria resistance variants, many of which are “loss of function” mutants. Evidence that this selection is recent comes from the genetic dating of the age of a number of these malaria resistant alleles to less than 5,000 years before the present, generally much more recent than other human genetic variants. An approach to estimate selection coefficients from contemporary case–control data is presented. In the situations described here, selection is much greater than 1%, significantly higher than generally observed for other human genetic variation. With these selection coefficients, predictions are generated about the joint change of alleles S and C at the β-globin locus, and for α-thalassaemia haplotypes and S, variants that are unlinked but exhibit epistasis. Population genetics can be used to determine the amount and pattern of selection in the past and predict selection in the future for other malaria resistance variants as they are discovered.
Malaria is one of the leading causes of death worldwide and has been suggested as the most potent type of selection in humans in recent millennia . As a result, genes involved in malaria resistance are excellent examples of recent, strong selection. Perhaps best known is the sickle cell haemoglobin variant, which is often used as an example of heterozygote advantage. In addition, G6PD deficiency illustrates strong selection at an X-linked locus, β-globin variants S, C, and E and G6PD deficiency variants A-, Med, and Mahidol show how selective differences can be the result of a single-nucleotide change. Further, HLA-B53 illustrates how gene conversion can result in an adaptive allele, and α-thalassaemia shows how selection can operate on loci that have different copy numbers.
Haldane, known as one of the three founders of population genetics, is often recognized with first suggesting that disease could be an important evolutionary force in humans. Although his general review  is often cited for this concept, this hypothesis was presented in more detail in 1948  where he suggested that β-thalassaemia heterozygotes had an increased fitness in the presence of malaria. Therefore, citation of  seems more correct for the hypothesis that malaria resistance in humans might be genetically determined and evolutionarily significant [4–6].
The first generally recognized evidence for genetic resistance to malaria in humans was in 1954  for sickle-cell haemoglobin heterozygotes AS. Overall, the “malaria hypothesis” of Haldane that some human diseases such as thalassaemia are polymorphisms and provide heterozygote advantage because of the trade-offs between the advantages of resistance to malaria and negative effects due to the disease, is now widely accepted but the exact means of disease resistance have often been difficult to elucidate.
As documentation for the contemporary influence of malaria, in 2010 there were 216 million clinical cases and an estimated 863,000 deaths from malaria , making it one of the leading causes of death worldwide. Although these levels have declined globally 25% since 2000, another analysis estimated that the annual mortality may be much higher than this level at 1.24 million .
The impact of malaria is thought to have increased between 10,000 and 5,000 years ago when there were the beginnings of agriculture and consequently more human settlements. During this period, the numbers of both the human population and the mosquito vector increased, resulting in higher spread of malaria . Recent molecular studies suggest that malaria in humans from Plasmodium falciparum may have originated from gorillas [11, 12]. Using these data, an initial timeline for the origin of P. falciparum as a human pathogen suggests that it may be more recent than previously thought .
The past geographic extent of malaria and the distribution of malaria resistance variants broadly correspond [14–16]. For example, P. falciparum is found across Africa and Asia, as are the variants of haemoglobin and G6PD that provide malaria resistance. In regions of high endemic malaria, such as sub-Saharan tropical Africa and lowland Melanesia, there are often several variants. In contrast, variants do not exist in areas without past malaria, with the exception of ancestry from new immigrants . To illustrate, the common malaria resistant alleles are not present in New World natives , presumably because their ancestors were unexposed to malaria and malaria only came to the Americas during the transatlantic slave trade between the 16th and 19th centuries . Further, microgeographic variation of β-thalassaemia in Sardinia and the past presence of malaria are concordant  and α-thalassaemia and β-thalassaemia variation in Melanesia is associated with malaria presence [21, 22].
Some resistance alleles for malaria are distinctive and others are very general. To illustrate, geographically separated Duffy alleles in Papua New Guinea and Africa result from changes at the same exact genomic location, 33 nucleotides upstream from the start codon. Similarly, the β-globin malaria resistance alleles S and C occur from different changes at the same codon. In contrast, there are many changes that modify levels of expression and provide malaria resistance for G6PD deficiency, α-thalassaemia, and β-thalassaemia.
It is significant that malaria resistance genes are often extremely variable, for example, the malaria resistance genes ABO, G6PD, HLA, α-globin, and β-globin, are some of the most variable human genes. Such variation might be because disease resistance genes have high amounts of standing variation or because these genes have high mutation rates and produce new adaptive alleles very quickly.
There are several current first-rate reviews of the mechanisms of malaria resistance and evaluations of the malaria resistance genes [1, 23–26] and in an extensive recent review, many aspects of the population genetics of malaria resistance were examined . Therefore, the focus here will be on the data indicating the very strong and recent selection for malaria resistance in humans. In addition, it will be shown how the expected change when there is strong selection for malaria resistance variants can be predicted with population genetics models.
Strength of selection
Pleiotropic disease effects
Strong selection for malaria resistance has increased, due to pleiotropic effects, the frequency of genetic diseases, such as G6PD deficiency, the thalassaemias, and ovalocytosis. For example, it has been suggested that this has caused a worldwide problem and has resulted in 300,000 newborns each year with haemoglobin maladies . These pleiotropic effects explain why such disorders are the most common genetic diseases . It is also possible that some other diseases may be in high frequency because of the pleiotropic effects of malaria resistance variants .
Around 400 million people (worldwide prevalence of 4.9%) are affected with G6PD deficiency, the most common enzymopathy in humans . Even though people with G6PD deficiency are often asymptomatic, various factors that result in oxidative stress can cause disease episodes. To illustrate, fava beans can cause severe haemolytic anaemia (favism) for individuals with the Med G6PD deficiency.
Perhaps related to the high frequency of diseases related to genes conferring resistance to malaria is that many of resistance variants, such as G6PD deficiency, the thalassaemias, sickle-cell anaemia, and ovalocytosis, are “loss of function” mutants and lead to reduced expression or altered gene product. Sometimes these variants result in disease because of their pleiotropic effects. Or, in other situations, there is no cost to the change in expression or gene product, as appears to be the case for the Duffy “null” variants and for β-globin gene allele C.
Age of selective variants
Estimated age of malaria resistance variants in years (assuming a 25-year generation length) and the selection coefficient (estimated or assumed), --- indicates no selection (neutrality) is assumed
The age of S alleles has been estimated from the amount of association (linkage disequilibrium) in sequence near the HBB locus. To illustrate, all samples of S from eastern Senegal had the same haplotype . Assuming that selection for AS was 0.152 higher than for AA, the estimated age of this S allele was 1,440 years (see Table 1) . Assuming that this allele was in low frequency (standing variation), it is likely that this age indicates the number of years since selection started to increase this allele.
Overall, there might have been five different mutations originating the S allele from the A allele. This conclusion is based on the presence of these variants in almost non-overlapping geographic regions, one found in both India and Saudi Arabia and four others in different parts of Africa, and the large differences in DNA sequence for genetic regions linked to S, resulting in different haplotypes in different geographic regions. The Mediterranean S alleles appear related to the African S haplotypes, particularly the Benin haplotype . Recent parallel adaptation of different S alleles is consistent with an encompassing population genetics model .
In addition, Table 1 gives the estimated or assumed selection coefficient for different variants. For example, a selection coefficient of 0.152 for S indicates that the sickle-cell heterozygote AS has a 15.2% advantage over the normal homozygote AA. As a comparison, a selective advantage of more than a few per cent is unusual for other human genes.
A joint estimate of the age of the C allele and the selection coefficient was approximated with a coalescent approach . The allele age was estimated to be 2,810 years and a selection coefficient of 0.06. More than one mutational origin for the E allele in Thailand is indicated . On the other hand, the Chinese E allele has the same haplotype as in Thailand , implying the same origin. The age of the E allele was estimated to be about 2,510 years  and selection of 0.079.
Different ways have been used to estimate the age of G6PD mutants (Table 1). Using simulations, estimation of the age and selection for the A- allele was 6,360 years with selection of 0.044 and for the Med allele was 3,330 years and selection of 0.034 . Using Bayesian methods, the estimated age of A- was only 1,000 years and selection was 0.25  and the age of the Mahidol allele was 1,580 years with selection of 0.23 . These estimates may differ because those in  used microsatellite loci and those in  used sequence data.
The age of the HLA-Bw53 variant can be estimated using the approach below to estimate s (with OR = 0.59, m = 0.1, so that s = 0.041) and iteration of population genetic equations. Therefore, it would take approximately 2,150 years for Bw53 to reach 0.25 . This time is substantially less than the time that strong selection in Africa for malaria resistance is thought to have occurred.
There are several other malaria resistant variants that appear to be recent but for which there has been no formal estimation of their age. For example, out of about 80 deletions that result in α+ thalassaemia, four common ones have different geographic distributions; for example, deletion –α3.7 is found nearly worldwide but is most common in Africa, deletion –α4.2 has a high frequency in southeast Asia, and – α3.7I is found most commonly in African, Indian and Mediterranean populations . In addition, there are a number of mutants that have very limited geographic distributions. The age of these mutants has not been estimated, but their narrow distributions indicate recent generation.
Most of the β-thalassaemias are the result of single nucleotide substitutions or small insertions or deletions. Around 200 β-thalassaemias variants are known and they also have narrow geographic distributions suggesting that these changes indicate independent mutations and have been recently generated. β-thalassaemia variants in carriers are in frequencies from 5 to 20%, somewhat lower in frequency than α- thalassaemia variants .
A mutation in gene SLC4A1 (erythrocyte Band 3 protein gene) found in Papua New Guinea and Malaysia results from a 27-nucleotide (9-amino acid) deletion. This genetic region is conserved throughout species  and the mutant causes abnormal ovalocytosis, an atypical red blood cell shape, and anaemia in heterozygotes. Even in progeny from matings between heterozygotes, homozygotes have not observed, indicating they are lethal . The lethality of homozygotes and the high resistance of heterozygotes to malaria suggest that this variant has properties not very different from the S allele because the advantage to heterozygotes in resistance offsets the cost in fitness in homozygotes. Because of its limited distribution, it is thought to be a recent mutation.
There are several variants that provide resistance to malaria that appear to be older. For example, there are two antigens A and B produced by alleles FY*A and FY*B at the Duffy blood group locus [46, 47]. Many African-Americans and Africans do not have either allele and have another allele, known generally as a Duffy null allele. This allele, FY*B ES , is in very high frequency in most of Africa and provides resistance to malaria species Plasmodium vivax, but not to P. falciparum.
The estimated age of the FY*B ES allele, based on sequence analysis, is around 33,000 years , while the estimated age of this allele using microsatellite data is around 10,000 years ago . The earlier estimated origin may be more consistent with the early impact of P. vivax on human mortality. Surprisingly, a Duffy allele in low frequency (0.022) in Papua New Guinea, also differs from FY*A in the same exact sequence as does the FY*B ES allele found in Africa . The low frequency of this allele is probably because of its recent origin, a situation seen in the pattern of alleles at closely linked loci .
Examples of strong selection and change in allele frequency
As illustration, here two examples are given of fast expected genetic changes over time due to strong selection at genes conferring resistance to malaria. Both examples are ones in which there are two resistant variants in one population, one in which the genetic variants are different alleles at the same gene (S and C) and the other where the genetic variants are different alleles at different genes but there is epistasis between these variants (S and α-thalassaemia). Before these examples are described, an approach will be briefly introduced to estimate the extent of selection at these variants using contemporary case–control data (see  for details).
Estimation of selection
A general approach to determine the risk of individuals with a given genotype getting a disease, relative to that in the rest of the population, is to calculate the odds ratio (OR) as OR = f d (1 – f c )]/f c (1 - f d )] where the frequencies of the genotype in control and diseased groups are f c and f d , respectively. For example, the selection coefficient for the AS heterozygote can be estimated as s = m(1 – OR) where m is the rate of non-genotype specific mortality from malaria (generally the mortality given that an individual has severe malaria) [52, 53].
To illustrate how OR and s can be calculated, data from a large study of malaria in The Gambia can be used to determine the effect of sickle-cell variation on the presence of severe malaria . In 619 children with severe malaria, only seven (0.012) were heterozygous AS. On the other hand, in 510 other children, who were outpatients and termed “mild controls”, many more 66 (0.129) were AS. Therefore, OR = 0.082, indicating very great protection from severe malaria for AS genotypes. A value of 0.07 for m was suggested  while, a value of at least m = 0.1 may be appropriate [10, 55]. For the AS genotype, if OR = 0.082 and m = 0.1, then s = 0.092.
Increase of allele C in the presence of allele S
The polymorphic structural variants at the β-globin locus, S and C, both are malaria resistance alleles. Variant S is more widespread than C, which is in sub-Saharan Africa, but they both occur in a number of populations. Although some early studies suggested a stable polymorphism of these variants, a Burkina Faso sample  indicated the C allele would become fixed over time [53, 57].
Frequency of different genotypes in healthy (control) subjects ( f c ) and in malaria (diseased) patients ( f d ) []
These results occur because genotype CC has the highest relative fitness of the genotypes. In addition, it might clarify situations, such as in the Dogon people of Mali who have a high frequency of C and a low S frequency . In addition, and of public health consequence, the mean fitness of the population is about 11% higher when fixed for C than when it is polymorphic for A and S because of the absence of sickle-cell anaemia and higher resistance to malaria.
Effect of epistasis between alleles S and α + - thalassaemia
Sickle cell anaemia and α+-thalassaemia both occur in some sub-Saharan African populations. The adult haemoglobin molecule is composed for two subunits of each of the α-globin and β-globin molecules. As a result, it is not unexpected that there could be gene-gene interaction, or epistasis, at these loci for fitness. In a Kenyan population, these disorders were both present  and when the two disorders were inherited together in the same individual, the protection from malaria given individually by each allele was lost (see also ). This interaction, or negative epistasis, could be part of the explanation why α+-thalassaemia does not appear fixed in sub-Saharan populations. A traditional population genetics approach is used here to examine this situation, for an epidemiological approach, see [59, 61, 62]
This array of relative fitness values illustrates several things. First, there is a stable polymorphism with S equal to 0.309 when only the normal αα haplotype at the a-globin gene is present (first column). Second, variant –α haplotype would be fixed when only the normal A allele is present at the β-globin locus (first row) because the –α/−α genotype has the highest fitness. In addition, some two-locus behaviour can be intuited by looking at these viability values further. For example, if the population is fixed for –α/–α it does not appear that S can enter the population because AA –α/−α > AS −α/−α (Table 3).
Obviously, Haldane  was correct to point out that malaria resistance in humans was a significant evolutionary factor. Malaria resistance genes comprise some of the most widely accepted examples of strong positive selection in humans. Overall, the original “malaria hypothesis” of Haldane that diseases like thalassaemia are polymorphisms because of resistance to malaria, has been proven correct. However, much is still to be learned about actual mechanisms of protection, other genes that confer resistance, and the population genetics of this variation.
Malaria resistance offers cases of a number of aspects of population genetics, particularly situations of recent, strong selection. Overall, many of these resistance variants appear less than 5,000 years old, much more recent than for most human variants. They also have selection coefficients significantly larger than 1%, much stronger than for most human variants. Population genetics will probably be even more important in understanding the joint impact of multiple malaria resistance variants in the future. Two situations are examined here, and it is shown that in populations segregating for S and C, selection is expected to eliminate S and fix C. Also, depending upon the starting frequencies, in populations segregating for α-thalassaemia and S, either the α -thalassaemia haplotype will become fixed and the S allele eliminated or a stable equilibrium of both variants will occur because of negative epistasis.
This research was partially supported by the Ullman Distinguished Professorship.
- Kwiatkowski DP: How malaria has affected the human genome and what human genetics can teach us about malaria. Amer J Hum Genet. 2005, 77: 171-192. 10.1086/432519.PubMed CentralView ArticlePubMedGoogle Scholar
- Haldane JBS: Disease and evolution. La Ricerca Scientifica. 1949, 19: 68-76.Google Scholar
- Haldane JBS: The rate of mutation of human genes. Hereditas. 1949, 35 (S1): 267-273.View ArticleGoogle Scholar
- Crow JF: J. B. S. Haldane’s ideas in biology with special reference to disease and evolution. Infectious Disease and Host-Pathogen Evolution. Edited by: Dronamraju KR. 2004, Cambridge: Cambridge University Press, 11-17.View ArticleGoogle Scholar
- Weatherall DJ: J. B. S. Haldane and the malaria hypothesis. Infectious Disease and Host-Pathogen Evolution. Edited by: Dronamraju KR. 2004, Cambridge: Cambridge University Press, 18-36.View ArticleGoogle Scholar
- Hedrick PW: Population genetics of malaria resistance in humans. Heredity. 2011, 107: 283-304. 10.1038/hdy.2011.16.PubMed CentralView ArticlePubMedGoogle Scholar
- Allison AC: Protection afforded by the sickle-cell trait against subtertian malaria infection. Brit Med J. 1954, 1: 290-294. 10.1136/bmj.1.4857.290.PubMed CentralView ArticlePubMedGoogle Scholar
- WHO: World Malaria Report. 2011, Geneva: World Health OrganizationGoogle Scholar
- Murray CJL, Rosenfeld LC, Lim SS, Andrews KG, Foreman KJ, Haring D, Fullman N, Lozano NM, Lozano R, Lopez AD: Global malaria mortality between 1980 and 2010: a systematic analysis. Lancet. 2012, 379: 413-431. 10.1016/S0140-6736(12)60034-8.View ArticlePubMedGoogle Scholar
- Carter R, Mendis KN: Evolutionary and historical aspects of the burden of malaria. Clin Micro Rev. 2002, 15: 564-594. 10.1128/CMR.15.4.564-594.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Liu W, Li V, Rudicell RS, Robertson JD, Keele BF, Ndjango J-B N, Sanz CM, Morgan DB, Locatelli S, Gonder MK, Kranzusch PJ, Walsh PD, Delaporte E, Mpoudi-Ngole E, Georgiev AV, Muller MN, Shaw GM, Peeters M, Sharp PM, Rayner JC, Hahn BH: Origin of the human malaria parasite Plasmodium falciparum in gorillas. Nature. 2010, 467: 420-425. 10.1038/nature09442.PubMed CentralView ArticlePubMedGoogle Scholar
- Rayner JC, Liu W, Peeters M, Sharp PM, Hahn BH: A plethora of Plasmodium species in wild apes: a source of human infection?. Trends Parasit. 2011, 27: 222-229. 10.1016/j.pt.2011.01.006.View ArticleGoogle Scholar
- Baron JM, Higgins JM, Dzik WH: A revised timeline for the origin of Plasmodium falciparum as a human pathogen. J Molec Evol. 2011, 73: 297-304. 10.1007/s00239-011-9476-x.View ArticlePubMedGoogle Scholar
- Flint J, Harding RM, Boyce AJ, Clegg JB: The population genetics of the haemoglobinopathies. Bailliere’s Clin Haemat. 1998, 11: 1-51. 10.1016/S0950-3536(98)80069-3.View ArticleGoogle Scholar
- Piel FB, Patil AP, Howes RE, Nyangiri OA, Gething PW, Williams TN, Weatherall DJ, Hay SI: Global distribution of the sickle cell gene and geographical confirmation of the malaria hypothesis. Nat Commun. 2010, 1: 104-10.1038/ncomms1104.PubMed CentralView ArticlePubMedGoogle Scholar
- Howes RE, Patil AP, Piel FB, Nyangiri OA, Kabaria CW, Gething PW, Zimmerman PA, Bernades C, Beall CM, Gebremedhin A, Ménard D, Williams TN, Weatherall DJ, Hay SI: The global distribution of the Duffy blood group. Nat Commun. 2011, 2: 226-View ArticleGoogle Scholar
- O’Shaughnessy DF, Hill AVS, Bowden DK, Weatherall DJ, Clegg JB: Globin genes in Micronesia: origins and affinities of Pacific Island peoples. Amer J Hum Genet. 1990, 46: 144-155.PubMed CentralPubMedGoogle Scholar
- Livingstone FB: Frequencies of Haemoglobin Variants. 1985, Oxford: Oxford University PressGoogle Scholar
- Valcindag E, Elguero E, Arnathau C, Durand P, Aklana J, Anderson TJ, Aubouy A, Balloux F, Besnard P, Bogreau H, Canevale P, D’Alessandro U, Fontenille D, Gamboa D, Jombart T, Le Mire J, Leroy E, Maestre A, Mayxay M, Menard , Musset L, Newton PN, Nkoghe D, Noya O, Ollomo B, Rogier , Veron V, Wide A, Zakeri S, Carme B, Legrand E, Chevillon C, Ayala FJ, Renaud F, Prugnolle F: Multiple independent introductions of Plasmodium falciparum in South America. Proc Nat Acad Sci USA. 2012, 109: 511-516. 10.1073/pnas.1119058109.View ArticleGoogle Scholar
- Siniscaloco M, Bernini L, Latte B, Motulsky AG: Favism and thalassemia in Sardinia and their relationship to malaria. Nature. 1961, 190: 1179-1180. 10.1038/1901179a0.View ArticleGoogle Scholar
- Flint J, Hill AV, Bowden DK, Oppenheimer SJ, Sill PR, Serjeantson SW, Bana-Koiri J, Bhatia K, Alpers MP, Boyce AJ, Weatherall DJ, Clegg JB: High frequencies of alpha-thalassemia are the result of natural selection by malaria. Nature. 1986, 321: 744-750. 10.1038/321744a0.View ArticlePubMedGoogle Scholar
- Hill AVS, Bowden DK, O’Shaughnessy DF, Weatherall DJ, Clegg JB: b-thalassemia in Melanesia: association with malaria and characterization of a common variant (IVS1 nt 5 G-C). Blood. 1988, 72: 9-14.PubMedGoogle Scholar
- Kwiatkowski DP, Luoni G: Malaria. Genetic Susceptibility to Infectious Diseases. Edited by: Kaslow RA, McNicholl JM, Hill AVS. 2008, Oxford: Oxford University Press, 372-386.Google Scholar
- Verra F, Mangang VD, Modiano D: Genetics of susceptibility to Plasmodium falciparum: from classical malaria resistance genes towards genome-wide association studies. Parasite Immun. 2009, 31: 234-253. 10.1111/j.1365-3024.2009.01106.x.View ArticleGoogle Scholar
- López C, Saravia C, Gomez A, Hoebeke J, Patarroyo MA: Mechanisms of genetically-based resistance to malaria. Gene. 2011, 467: 1-12.View ArticleGoogle Scholar
- Driss A, Hibbert JM, Wilson NO, Iqbal SA, Adamkiewicz TV, Stiles JK: Genetic polymorphisms linked to susceptibility to malaria. Malar J. 2011, 10: 271-10.1186/1475-2875-10-271.PubMed CentralView ArticlePubMedGoogle Scholar
- Weatherall DJ: The inherited diseases of hemoglobin are an emerging global health burden. Blood. 2010, 115: 4331-4336. 10.1182/blood-2010-01-251348.PubMed CentralView ArticlePubMedGoogle Scholar
- Weatherall DJ: Genetic variation and susceptibility to infection: the red cell and malaria. Brit J Haemat. 2008, 141: 276-286. 10.1111/j.1365-2141.2008.07085.x.View ArticlePubMedGoogle Scholar
- Nkhoma ET, Poole C, Vannappagari V, Hall SA, Beutler E: The global prevalence of glucose-6-phosphate dehydrogenase deficiency: A systematic review and meta-analysis. Blood Cells Mol Dis. 2009, 42: 267-278. 10.1016/j.bcmd.2008.12.005.View ArticlePubMedGoogle Scholar
- Verrelli BC, Tishkoff SA, Stone AC, Touchman JW: Contrasting histories of G6PD molecular evolution and malarial resistance in humans and chimpanzees. Molec Biol Evol. 2006, 23: 1592-1601. 10.1093/molbev/msl024.View ArticlePubMedGoogle Scholar
- MacFie TS, Nerrienet E, Bontrop RE, Mundy NI: The action of falciparum malaria on the human and chimpanzee genomes compared: Absence of evidence for a genomic signature of malaria at HBB and G6PD in three subspecies of chimpanzee. Infect Genet Evol. 2009, 9: 1248-1252. 10.1016/j.meegid.2009.06.025.View ArticlePubMedGoogle Scholar
- Demogines A, Truong KA, Sawyer SL: Species-specific features of DARC, the primate receptor for Plasmodium vivax and Plasmodium knowlesi. Molec Biol Evol. 2011, 29: 445-449.PubMed CentralView ArticlePubMedGoogle Scholar
- Tung J, Primau A, Bouley AJ, Severson TF, Alberts SC, Wray GA: Evolution of a malaria resistance gene in wild primates. Nature. 2009, 460: 388-391.PubMedGoogle Scholar
- Currat M, Trabucher G, Rees D, Perrin P, Harding RM, Clegg JB, Langaney A, Excoffier L: Molecular analysis of the β-globin gene cluster in the Niokholo Mandenka population reveals a recent origin of the βS Senegal mutation. Amer J Hum Genet. 2002, 70: 207-223. 10.1086/338304.PubMed CentralView ArticlePubMedGoogle Scholar
- Ralph P, Coop G: Parallel adaptation: one or many waves of advance of an advantageous allele?. Genetics. 2010, 186: 647-668. 10.1534/genetics.110.119594.PubMed CentralView ArticlePubMedGoogle Scholar
- Wood ET, Stover DA, Slatkin M, Nachman MW, Hammer MF: The β-globin recombinational hotspot reduces the effects of strong selection around HbC, a recently arisen mutation providing resistance to malaria. Amer J Hum Genet. 2005, 77: 637-642. 10.1086/491748.PubMed CentralView ArticlePubMedGoogle Scholar
- Ohashi J, Naka I, Patarapotikul J, Hananantachai H, Brittenham , Looareesuwan S, Clark AG, Tokunaga K: Strong linkage disequilibrium of a HbE variant with the (AT)9(T)5 repeat in the BP1 binding site upstream of the β-globin gene in the Thai population. J Hum Genet. 2005, 50: 7-11. 10.1007/s10038-004-0210-z.PubMed CentralView ArticlePubMedGoogle Scholar
- Fucharoen G, Fucharoen S, Sanchaisuriya K, Sae-ung N, Suyasunanond U, Sriwilai , Chinorak P: Frequency distribution and haplotypic heterogeneity of βE-globin gene among eight minority groups of northeast Thailand. Hum Hered. 2002, 53: 18-22. 10.1159/000048600.View ArticlePubMedGoogle Scholar
- Ohashi J, Naka I, Patarapotikul J, Hananantachai H, Brittenham G, Looareesuwan S, Clark AG, Tokunaga K: Extended linkage disequilibrium surrounding the hemoglobin E variant due to malarial selection. Amer J Hum Genet. 2004, 74: 1198-1208. 10.1086/421330.PubMed CentralView ArticlePubMedGoogle Scholar
- Tishkoff SA, Varkonyl R, Cahinhinan N, Abbes S, Argyropoulos G, Destro-Bisol G, Drousiotou A, Dangerfield B, Lefranc G, Loiselet J, Piro A, Stoneking M, Tagarelli A, Tagarelli G, Touma EH, Williams SM, Clark AG: Haplotype diversity and linkage disequilibrium at human G6PD: recent origin of alleles that confer malarial resistance. Science. 2001, 293: 455-462. 10.1126/science.1061573.View ArticlePubMedGoogle Scholar
- Slatkin M: A Bayesian method for jointly estimating allele age and selection intensity. Genet Res. 2008, 90: 129-137.View ArticleGoogle Scholar
- Louicharoen C, Patin E, Paul R, Nuchprayoon I, Witoonpanich B, Peerapittayamongkol C, Casademont I, Sura T, Laird NM, Singhasivanon P, Quintana-Murci L, Sakuntabhai A: Positively selected G6PD-Mahidol mutation reduces Plasmodium vivax density in southeast Asian. Science. 2009, 326: 1546-1549. 10.1126/science.1178849.View ArticlePubMedGoogle Scholar
- Weatherall DJ, Clegg JB: The Thalassemia Syndromes. 2001, Oxford: Blackwell Scientific, 4View ArticleGoogle Scholar
- Jarolim P, Palek J, Amato D, Hassan K, Sapak P, Nurse GT, Rubin HL, Zhai S, Sahr KE, Liu S-C: Deletion in erythrocyte band 3 gene in malaria–resistant Southeast Asian ovalocytosis. Proc Nat Acad Sci USA. 1991, 88: 11022-11026. 10.1073/pnas.88.24.11022.PubMed CentralView ArticlePubMedGoogle Scholar
- Genton B, Al-Yaman F, Mgone CS, Alexander N, Paniu MM, Alpers MP: Ovalocytosis and cerebral malaria. Nature. 1995, 378: 564-565. 10.1038/378564a0.View ArticlePubMedGoogle Scholar
- Zimmerman PA: The enigma of Plasmodium vivax malaria and erythrocyte Duffy negativity. Infectious Disease and Host-Pathogen Evolution. Edited by: Dronamraju KR. 2004, Cambridge: Cambridge University Press, 141-172.View ArticleGoogle Scholar
- Mercereau-Puijalon O, Ménard D: Plasmodium vivax and the Duffy antigen: A paradigm revisited. Transfus Clin Biol. 2010, 17: 176-183. 10.1016/j.tracli.2010.06.005.View ArticlePubMedGoogle Scholar
- Miller LH, Mason SJ, Clyde DF, McGinnis MH: The resistance factor to Plasmodium vivax in blacks. The Duffy-blood-group genotype FyFy. New England J Med. 1976, 295: 302-304. 10.1056/NEJM197608052950602.View ArticleGoogle Scholar
- Hamblin MT, Di Rienzo A: Detection of the signature of natural selection in humans: evidence from the Duffy blood group locus. Amer J Hum Genet. 2000, 66: 1669-1679. 10.1086/302879.PubMed CentralView ArticlePubMedGoogle Scholar
- Seixas S, Ferrand N, Rocha J: Microsatellite variation and evolution of the human Duffy blood group polymorphism. Molec Biol Evol. 2002, 19: 1802-1806. 10.1093/oxfordjournals.molbev.a004003.View ArticlePubMedGoogle Scholar
- Zimmerman PA, Woolley I, Masinde GL, Miller SM, McNamara D, Hazlett F, Mgone CS, Alpers MP, Genton B, Boatin BA, Kazure JW: Emergence of FY*null in a Plasmodium vivax-endemic region of Papua New Guinea. Proc Nat Acad Sci USA. 2009, 96: 13973-13977.View ArticleGoogle Scholar
- Hill AVS: HLA associations with malaria in Africa: some implications for MHC evolution. Molecular Evolution of the Major Histocompatibility Complex. Edited by: Klein J, Klein D. 1991, Berlin: Springer, 403-434.View ArticleGoogle Scholar
- Hedrick PW: Estimation of relative fitnesses from relative risk data and the predicted future of haemoglobin alleles S and C. J Evol Biol. 2004, 17: 221-224.View ArticlePubMedGoogle Scholar
- Hill AVS, Allsopp CEM, Kwiatkowski D, Anstey NM, Twumasi P, Rowe PA, Bennett S, Brewster D, McMichael AJ, Greenwood BM: Common west African HLA antigens are associated with protection from severe malaria. Nature. 1991, 352: 595-600. 10.1038/352595a0.View ArticlePubMedGoogle Scholar
- WHO: Demographic data for health situation assessment and projections. 1998, Geneva: WHO HST/HSPGoogle Scholar
- Modiano D, Luoni G, Sirima BS, Simpore J, Verra F, Konate A, Rashtrelli , Oliveri A, Calissano C, Paganotti GM, D’Urbano L, Sanou I, Sawadogo A, Modiano G, Coluzzi M: Haemoglobin C protects against clinical Plasmodium falciparum malaria. Nature. 2001, 414: 305-308. 10.1038/35104556.View ArticlePubMedGoogle Scholar
- Modiano D, Bancone G, Ciminelli BM, Pompei F, Blot I: Haemoglobin S and haemoglobin C: ‘quick but costly’ versus ‘slow but gratis’ genetic adaptations to Plasmodium falciparum malaria. Hum Molec Genet. 2008, 17: 789-799.View ArticlePubMedGoogle Scholar
- Agarwal A, Guindo A, Cissoko Y, Taylor TG, Coulibaly D, Kone A, Kayentao K, Djimde A, Plowe CV, Documbo O, Wellems TE, Diallo D: Hemoglobin C associated with protection from severe malaria in the Dogon of Mali, a West African population with a low prevalence of hemoglobin S. Blood. 2000, 96: 2358-2363.PubMedGoogle Scholar
- Williams TN, Mwangi TW, Wambua S, Peto TEA, Weatherall DJ: Negative epistasis between the malaria-protective effects of α+-thalassemia and the sickle cell trait. Nat Genet. 2005, 37: 1253-1262. 10.1038/ng1660.PubMed CentralView ArticlePubMedGoogle Scholar
- May J, Evans JA, Timmann C, Ehmen C, Busch W, Thye T, Agbenyega T, Horstmann RD: Hemoglobin variants and disease manifestations in severe falciparum malaria. J Amer Med Assoc. 2007, 297: 2220-2226. 10.1001/jama.297.20.2220.View ArticleGoogle Scholar
- Penman BS, Pybus OG, Weatherall DJ, Gupta S: Epistatic interactions between genetic disorders of hemoglobin can explain why the sickle-cell gene is uncommon in the Mediterranean. Proc Nat Acad Sci USA. 2009, 106: 21242-21246. 10.1073/pnas.0910840106.PubMed CentralView ArticlePubMedGoogle Scholar
- Penman BS, Habib S, Kanchan K, Gupta S: Negative epistasis between a+ thalassaemia and sickle cell trait can explain interpopulation variation in south Asia. Evolution. 2011, 65: 3625-3632. 10.1111/j.1558-5646.2011.01408.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Hedrick PW: Genetics of Populations. 2011, Boston: Jones and Bartlett, 4Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.