Efficacy and safety of artemether-lumefantrine and dihydroartemisinin-piperaquine in the treatment of uncomplicated Plasmodium falciparum malaria in Kenyan children aged less than five years: results of an open-label, randomized, single-centre study
© Ogutu et al.; licensee BioMed Central Ltd. 2014
Received: 13 August 2013
Accepted: 7 January 2014
Published: 28 January 2014
This open-label, randomized study evaluated efficacy and safety of artemether-lumefantrine (AL) and dihydroartemisinin-piperaquine (DP) in treatment of uncomplicated falciparum malaria in children below five years of age, to build evidence on use of AL as first-line treatment and DP as second-line treatment in Kenya.
A total of 454 children aged six to 59 months with uncomplicated falciparum malaria were randomized (1:1) to receive AL dispersible or DP paediatric tablets and followed up for 42 days. Primary efficacy variable was corrected adequate clinical and parasitological response (ACPR) rate on day 28. Secondary variables included corrected (day 14, 28 and 42), uncorrected (day 3, 14, 28 and 42) cure rates, parasitological failure at days 3, 14 and 42. Acceptability and tolerability of both drugs were assessed by caregiver questionnaire.
On day 28, corrected ACPR rates for AL dispersible and DP paediatric were 97.8% (95% CI: 94.9-99.3) and 99.1% (95% CI: 96.8-99.9), respectively, in intention-to-treat population, with no significant treatment differences noted between AL dispersible and DP paediatric arms. Additionally, no significant differences were observed for PCR corrected cure rates on days 14 and ACPR on day 42 for AL dispersible (100%; 96.8%) and DP paediatric (100%; 98.7%). Similarly, for PCR uncorrected cure rates, no significant differences were seen on days 3, 14, 28, and 42 for AL dispersible (99.1%; 98.7%; 81.1%; 67.8%) and DP paediatric (100%; 100%; 87.7%; 70.5%). Parasite clearance was rapid, with approximately 90% clearance achieved in 40 hours in both treatment arms. Incidence of adverse events was related to underlying disease; malaria being reported in both treatment arms. One serious adverse event was noted in AL dispersible (0.42%) arm, not related to study drug. Adherence to treatment regimen was higher for children treated with AL dispersible (93.6%) compared to DP paediatric (85.6%). Acceptability of AL dispersible regimen was assessed as being significantly better than DP paediatric.
AL and DP were both efficacious and well tolerated, and had similar effects at day 42 on risk of recurrent malaria. No signs of Plasmodium falciparum tolerance to artemisinins were noted.
Malaria continues to be a major health problem worldwide, with an estimated 216 million cases in 2010, approximately 174 million of which were in the African region. Children less than five years of age are at increased risk of Plasmodium falciparum malaria, with a reported 86% mortality rate globally. Artemisinin-based combination therapy (ACT) is recommended as the first-line treatment for uncomplicated P. falciparum malaria by the World Health Organization (WHO), over chloroquine and sulpha drugs worldwide. Multidrug resistance has been reported for monotherapy (for example artemisinin[3–5]) and some of the available combination chemotherapy (for example, sulphadoxine/sulphalene-pyrimethamine) used for malaria. Treatment with ACT is known to improve cure rates, results in rapid parasite clearance[2, 7] and reduces gametocyte carriage resulting in a decrease in parasite transmission[2, 8–10].
Artemether-lumefantrine (AL, Coartem®, Novartis Pharma AG) is a first fixed-dose ACT, which meets the WHO prequalification criteria for efficacy, safety and quality, and is indicated for the treatment of uncomplicated falciparum malaria, or mixed infections including P. falciparum in adults, children, and infants (>5 kg body weight). Since the prequalification of Coartem®, other AL generics have been prequalified by WHO. In clinical studies, AL demonstrated consistent 28-day polymerase chain reaction (PCR)-corrected cure rates of more than 95% in adult and paediatric populations with a favourable safety and tolerability profile. With rapid parasite clearance and gametocyte reduction also being reported, the widespread adoption of AL has led to a significant contribution in the reduction of malaria burden in Sub-Saharan Africa. Since the Kenyan Ministry of Health introduced AL as the first-line treatment for uncomplicated falciparum malaria in 2006, there has been a notable reduction in child mortality rates. Novartis launched the paediatric formulation of AL (AL dispersible) in 2009, developed jointly with Medicines for Malaria Venture (MMV). It is a sweet-tasting and easy-to-administer tablet which is now approved in over 40 countries. AL dispersible has proven in clinical trials to be as well tolerated and as effective as regular AL tablets[14, 15]. Moreover, it has been shown that standard African diets (generally consisting of a carbohydrate staple supplemented by pulses, nuts, meat or fish and fat from oil crops or from vegetables or plants) and milk are adequate to ensure AL efficacy. However, AL is associated with a high risk of re-infection soon after therapy in high-transmission areas.
Dihydroartemisinin-piperaquine (DP: Duo-cotecxin®, Holley-Cotec Pharmaceuticals) is an ACT that is administered as a single daily dose over three days and has been shown to be well tolerated and highly effective against falciparum malaria. In a previous comparative assessment, DP has been proven to be as efficacious and safe as AL in the treatment of uncomplicated falciparum malaria in Zambian children less than five years of age. DP has been suggested for use as a rescue and/or alternative treatment to AL, or as second-line treatment following treatment failure with the initial treatment. The Kenyan Ministry of Health adopted DP as second-line treatment in 2009.
To further build on the evidence from earlier studies that used AL as a first-line and DP as second-line treatment, this study evaluated the efficacy and safety of AL dispersible vs DP paediatric in Kenyan children less than five years of age for the treatment of uncomplicated falciparum malaria.
This was an open-label, randomized, single-centre study (Trial registration number: PACTR201111000316370). The protocol was approved by the Kenya Medical Research Institute Ethics Review Committee. The study was conducted in accordance with the Declaration of Helsinki (2002), Good Clinical Practices guidelines set up by the International Conference on Harmonization, and local applicable laws and regulations.
Children aged six to 59 months (inclusive), weighing 5 kg or more with fever ≥37.5°C (axillary), who presented to the Ombeyi Dispensary with probable clinical malaria, mono-infection with P. falciparum at an asexual parasite density of 1,000-200,000 parasites/μL, and who were able to take drug orally, were eligible for enrolment in the study. Patients were excluded if they had severe and/or complicated malaria, including severe anaemia (haemoglobin (Hb) ≤5 g/dL), experienced two or more seizures in the previous 24 hours and hyperparasitaemia (parasites >200,000/μL), or had a general clinical condition requiring hospitalization. Patients who had concomitant infections/disease at the time of presentation, with past or present history of chronic illnesses or any other underlying illness that would compromise the diagnosis and evaluation of the response to the study drug, with a history of allergy to artemisinin, lumefantrine or piperaquine were excluded. Patients undergoing full treatment with other anti-malarial drugs within the previous 14 days were also excluded. Eligible patients were enrolled after the parent/guardian signed the written informed consent.
The study comprised a three-day treatment period (day 1 taken as the first day of treatment) and 42-day follow-up. Eligible patients were randomized to one of the two treatment arms (1:1) to receive either AL dispersible (six doses/three days) or DP paediatric (three doses/three days). Patients in the AL dispersible arm with body weight 5–14 kg received one tablet (artemether 20 mg, lumefantrine 120 mg) per dose, and 15–24 kg received two tablets per dose. Patients in the DP paediatric arm received the standard dosage of 2.25 mg/kg and 18 mg/kg per dose of dihydroartemisinin and piperaquine, respectively, rounded up to the nearest half tablet. The drugs were dispersed in a small volume of water or milk and administered by the parents/caregivers under the observation of the study personnel. If there was vomiting within 30 minutes of the dose then the child was re-dosed and if the child vomited the replacement dose, he/she was dropped from the study. All the drug administration occurred in an inpatient setting. Children who developed severe malaria were treated with quinine as per the national guidelines.
Children were admitted to hospital for the first three days for observed treatment and close monitoring. They were evaluated daily in the ward and eight-hourly blood slides were performed until two consecutive negative blood smears for asexual falciparum malaria were obtained. Children were discharged after they had a negative slide and were clinically stable. At discharge, the parent/caregiver completed a questionnaire on the treatment acceptability, and the children were followed up on days 7, 14, 28, 42 and any other day the child was unwell. During the visits, a blood slide was analysed for parasite quantification; blood was collected for a complete blood count (CBC) and a filter paper sample for genotyping in case of parasite re-appearance; physical examination was performed and vital signs, axillary temperature were recorded. Adverse events (AEs) and serious adverse events (SAEs) were recorded and monitored throughout the study.
The primary efficacy assessment included patients with PCR-corrected parasitaemia by day 28, i e, patients with an adequate clinical and parasitological response (ACPR). According to the WHO definition, ACPR is the absence of parasitaemia on day 28 or day 42 irrespective of axillary temperature, without previously meeting any of the early treatment failure (ETF), late treatment failure (LTF) or late parasitological failure (LPF) criteria. As per the WHO definition, ETF is development of danger signs of malaria or severe malaria on post treatment days 0, 1, 2 or 3, with evidence that the patient is symptomatic and parasitaemic to a greater degree than the value recorded at entry to the trial on day 2; parasitaemic on day 3 greater than 25% of the value recorded on entry to the trial and with axillary temperature ≥37.5°C. LTF is development of danger signs or severe malaria between post-treatment days 4 to 14 inclusive when: a) the patient has not previously met the criteria for ETF and, b) PCR analysis of markers including merozoite surface protein (MSP)-1, MSP-2 and glutamate-rich protein (GLURP) suggests that the parasites are unlikely to be a new infection, with evidence that the patient is symptomatic and any level of P. falciparum parasitaemia on any day between 4, 28 or day 42, inclusive with axillary temperature ≥37.5°C in patients not meeting ETF criteria earlier. LPF is presence of parasitaemia on any day between day 7 and day 28 (or day 42) with axillary temperature <37.5°C in patients who did not previously meet any of the criteria of ETF or late clinical failure.
The secondary efficacy and safety assessments included parasitological failure at days 3, 14 and 42, (defined as presence or absence of parasitaemia on assessment), determination of the level of adherence, ease of use, acceptability and adverse events (serious and non-serious) reported as an indicator of safety for both drugs among patients enrolled in the study.
Safety assessments included recording of adverse events (AEs, by system organ class and preferred terms), serious adverse events (SAEs), treatment emergent serious and non-serious adverse events (AEs occurring between day 1 and 35 of treatment period or AEs with unknown onset dates), and collection of clinical laboratory data for haematology and blood chemistry. According to the ICH guidelines, AEs are defined as any untoward medical occurrences in a patient administered a pharmaceutical product and which does not necessarily have a causal relationship to the treatment. SAEs are untoward medical occurances that at any dose, result in death, are life threatening, require hospitalization, prolongation of existing hospitalization or result in persistant, and significant disability, or is a congenital anomaly/birth defect. During the study period the area was under indoor residual spraying (IRS) for malaria as a pilot district in the deployment of IRS in high transmission areas by the Division of Malaria Control of the Ministry of Public Health and Sanitation, Kenya.
In this non-inferiority trial, it was estimated that 193 patients per arm were needed to complete the study, assuming that at day 28, the efficacies of AL dispersible and DP paediatric were 90%, with a power of 80%, and a two-sided significance level of 5%. Allowing for a 10% dropout rate, it was calculated that 233 patients per arm (426 in total) had to be randomized. Baseline data were summarized by continuous variables using mean (standard deviation (SD)) if normally distributed, tested using t-test or ANOVA where appropriate or using median and inter-quartile range and non-parametric testing if not normally distributed.
The efficacy variables were analysed on the intent-to-treat (ITT) and per protocol (PP) population. The ITT population included all patients who received at least one dose of study drug. Patients with a major protocol deviation were included in the analyses. The PP population excluded patients with predefined protocol violations and included patients who completed all visits as specified in the protocol; had no major protocol violation with regards to inclusion/exclusion criteria; did not take any prohibited concomitant medications during the treatment period. Patients who were withdrawn from the study due to an AE or lack of efficacy, or who were considered a treatment failure, were included in the PP population.
For the primary efficacy variable, the treatment effect was the difference in efficacy between the two arms at day 28. Missing data was analysed by using complete case analysis wherein patients with missing efficacy data were excluded from the analysis. For secondary efficacy analyses, the treatment effect was the difference in efficacy between the two treatment arms at days 3, 14 and 42. In the analyses of safety data, the biological parameter analysis by treatment regimen was assessed by the mean (95% confidence interval (CI)) change from baseline on day 28. The mean (SD) parameter value at day 28 was presented for each arm. Formal testing for a difference between treatments was done using ANOVA comparing average day 28 values, adjusting for baseline values. Adverse events (both serious and non-serious) have been tabulated using preferred terms by treatment arm in Medical MeDRA V. 12. Failure rate has also been presented based on parasite density at baseline.
This study was conducted at Ombeyi dispensary in Nyando District, Kenya. The first patient was enrolled on 5 March, 2010 and the last patient completed the study on 30 November, 2011.
Patient disposition, demographics, and baseline characteristics
Patient demographics and baseline characteristics
AL dispersible (n = 227)
DP paediatric (n = 227)
Parasite density (/μL), mean (95% CI)†
38,202.2 (32,180.0 to 45,351.3)
38,545.7 (32,615.8 to 45,553.8)
White blood cells (x103/μL)
Red blood cells (x106/μL)
Effect of parasite load at baseline on failure: day 28
Parasite load at baseline
Failure at day 28
≤50,000 (n = 223)
>50,000 (n = 219)
≤100,000 (n = 328)
>100,000 (n = 114)
Most frequent adverse events (≥2% in any treatment arm)
AL dispersible (n = 238)
DP paediatric (n = 231)
Patients with at least one adverse event
Loss of appetite
Biological parameter changes during treatment
Mean change (95% CI)
Mean change (95% CI)
1.6 (1.3 to 1.9)
1.7 (1.4 to 1.9)
White blood cells (X103/μL)
−0.8 (−1.5 to −0.1)
−0.5 (−1.0 to 0.3)
88.5 (74.1 to 102.8)
88.3 (76.2 to 102.8)
Red blood cells (X106/μL)
0.6 (0.4 to 0.8)
0.7 (0.6 to 0.9)
−14.4 (−17.2 to −11.6)
−16.6 (−19.4 to −13.9)
14.4 (11.3 to 17.4)
18.8 (16.0 to 21.6)
−0.2 (−0.7 to 0.2)
−0.02 (−0.10 to 0.07)
−0.08 (−0.19 to 0.03)
−0.4 (−0.89 to 0.06)
−0.78 (−1.54 to −0.02)
Drug questionnaire response
AL dispersible N = 126
DP paediatric N = 124
Regimen, median number of tablets required
Pack available for survey, n (%)
Acceptability and comprehension
Health worker explained how to administer drug, n (%)
Health worker used pictures to explain how to administer drug, n (%)
Pictures helped in understanding how to administer, n (%)
Difficulty/Ease of using drug
Very difficult, n (%)
Difficult, n (%)
Acceptable, n (%)
Simple, n (%)
Very simple, n (%)
Difficulty/Ease of using drug A compared to B
Very difficult, n (%)
Difficult, n (%)
Acceptable, n (%)
Simple, n (%)
Very simple, n (%)
Taste of medicine for child
Not at all, n (%)
Not very much, n (%)
Acceptable, n (%)
Liked it, n (%)
Liked it very much, n (%)
Liquid for dissolving tablet
Water, n (%)
Other, n (%)
Drug taken with a meal, n (%)
Prefer drug given as
Syrup, n (%)
Whole tablet which dissolves in small volume of water/milk, n (%)
Injection, n (%)
Other, n (%)
Prefer other anti-malarias, n (%)
Perceived adverse drug reactions
Anything unusual after medication given, n (%)
Concomitant medication given, n (%)
Adverse reaction solicited, n (%)
Caretaker reported ADR to health worker, n (%)
ADR/SAE completed based on events seriousness, n (%)
In the present study, AL dispersible and DP paediatric were found to be highly efficacious for the treatment of uncomplicated falciparum malaria in Kenyan children less than five years of age. The 28-day corrected ACPR rates in patients receiving AL dispersible were not significantly different to those seen in patients receiving DP paediatric. The corrected ACPR rates were similar to those previously reported in Kenya and other parts of Africa[19, 22]. This shows that despite scaled-up use of AL dispersible in Kenya, the AL is still efficacious as first-line treatment. In contrast to earlier findings[19, 23, 24], there were no significant treatment differences noted in uncorrected ACPR rates between the AL dispersible and DP paediatric arms at any of the time points assessed, despite the difference in half-life between lumefantrine (four to six days)[25, 26] and piperaquine (two to three weeks). This may be due to the intensity of transmission in this region.
The current study was conducted in children aged between six and 59 months in the meso-endemic area of Kenya, where the malaria prevalence rate has increased from 4% in 2007 to 8% in 2010. This high-risk population has less likelihood of semi-immunity, and hence the efficacy detected was not greatly influenced by previous exposure to malaria.
With widespread adoption and scaled-up use of AL dispersible and other ACT in many sub-Saharan African countries, there is a risk of development of resistance; however, so far parasite resistance has not been reported in this region. In the present study, the majority of the children had parasite clearance within 48 hours with AL and DP and the parasite clearance rate reported here was similar to previously reported rates[30, 31]. This demonstrates that the P. falciparum parasites in this region are still sensitive to artemisinin derivatives, unlike in some regions of Southeast Asia where delayed response to ACT has been reported[32, 33]. This study builds on evidence for clinical practice in Kenya and sub-Saharan African countries that AL dispersible remains efficacious as a first-line treatment for uncomplicated P. falciparum malaria.
Overall, both drugs were well tolerated by children. There was a comparable occurrence of AEs in the AL dispersible and DP pediatric arms, with the most commonly reported AEs being malaria and cough, in line with the previously published data[23, 34–38]. No deaths were reported. One SAE was reported in the AL dispersible arm (severe malaria) and this was not considered to be related to the study drug. No discontinuations due to the study drug were observed. One limitation of the study was that it was not blinded and there was therefore potential for bias regarding tolerability and efficacy assessments. This was mitigated with a blinded randomization procedure and with the parents being blinded regarding the assigned drug.
Both of the ACT studied here are fixed-dose formulations. AL dispersible is administered twice daily for three days, whereas DP paediatric is administered once daily for three days. AL dispersible should be administered with food or milk to enhance lumefantrine absorption. Although the above facts might suggest that better treatment acceptability and tolerability may be observed with DP paediatric compared with AL dispersible, according to the survey conducted in this study, acceptability and tolerability to treatment regimen was in fact higher in the AL dispersible arm. More respondents considered AL dispersible simple to use and to have a better taste. Most ACT need to be crushed and mixed with water or food. Since they have a bitter taste, children can expectorate the medicine and this may result in them not receiving the full therapeutic dose. With its palatable flavour (‘liked’ or ‘liked very much’ by 72% of respondents), AL dispersible may enhance adherence and improve therapeutic outcomes in children[39, 40]. Moreover, it has been demonstrated that with the content of standard African diets or milk optimal efficacy is achieved with fixed-dose AL (Coartem). Oral bioavalability of AL was shown to increase by 108% when a normal meal was taken close to AL dosing compared to a fasting condition. Another finding of note in the acceptability and tolerability survey was the strong preference expressed for whole tablets, which dissolve in a small volume of water/milk over syrup formulations. Previous research has shown that dry powder formulations intended for suspension in water are more often substandard, relative to tablets, and may contain ineffective or incorrect amounts of preservatives. DP may be a good alternative to AL or may have use as rescue medication, and advice should be given regarding the policy and clinical practice for deployment of DP as second-line therapy in the treatment of uncomplicated falciparum malaria in Kenya.
AL is efficacious and remains the first-line treatment option for uncomplicated falciparum malaria. Both drugs were well tolerated by children. DP has proved to be as efficacious as AL. Parasites in this area remain sensitive to artemisinins.
This work has been published with the permission of the Director of the Kenya Medical Research Institute. This study was funded by Division of Malaria Control of the Ministry of Health, Kenya through support from WHO country office and DFID. The authors thank the patients and their parents who took part and the staff at the Ombeyi Dispensary. The authors would like to thank Ubhayabharathi Gurunath, professional medical writer (Novartis) for editorial support.
- World Malaria Report: World Malaria Report. 2011,http://www.who.int/entity/malaria/world_malaria_report_2011/9789241564403_eng.pdf,Google Scholar
- World Health Organization: Guidelines for the Treatment of Malaria. 2010,http://www.who.int/malaria/publications/atoz/9789241547925/en/index.html, 2,Google Scholar
- Dondorp AM, Nosten F, Yi P, Das D, Phyo AP, Tarning J, Lwin KM, Ariey F, Hanpithakpong W, Lee SJ, Ringwald P, Silamut K, Imwong M, Chotivanich K, Lim P, Herdman T, An SS, Yeung S, Singhasivanon P, Day NP, Lindegardh N, Socheat D, White NJ: Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2009, 361: 455-67. 10.1056/NEJMoa0808859.PubMed CentralView ArticlePubMedGoogle Scholar
- Noedl H, Se Y, Schaecher K, Smith BL, Socheat D, Fukuda MM, Artemisinin Resistance in Cambodia 1 (ARC1) Study Consortium: Evidence of artemisinin-resistant malaria in western Cambodia. N Engl J Med. 2008, 359: 2619-20. 10.1056/NEJMc0805011.View ArticlePubMedGoogle Scholar
- Noedl H, Socheat D, Satimai W: Artemisinin-resistant malaria in Asia. N Engl J Med. 2009, 361: 540-541. 10.1056/NEJMc0900231.View ArticlePubMedGoogle Scholar
- Rønn AM, Msangeni HA, Mhina J, Wernsdorfer WH, Bygbjerg IC: High level of resistance of Plasmodium falciparum to sulfadoxine–pyrimethamine in children in Tanzania. Trans R Soc Trop Med Hyg. 1996, 90: 179-81. 10.1016/S0035-9203(96)90129-7.View ArticlePubMedGoogle Scholar
- Adjuik M, Babiker A, Garner P, Olliaro P, Taylor W, White N, International Artemisinin Study Group: Artesunate combinations for treatment of malaria: meta–analysis. Lancet. 2004, 363: 9-17.View ArticlePubMedGoogle Scholar
- Kokwaro G, Mwai L, Nzila A: Artemether/lumefantrine in the treatment of uncomplicated falciparum malaria. Expert Opin Pharmacother. 2007, 8: 75-94. 10.1517/14656522.214.171.124.View ArticlePubMedGoogle Scholar
- Targett G, Drakeley C, Jawara M, von Seidlein L, Coleman R, Deen J, Pinder M, Doherty T, Sutherland C, Walraven G, Milligan P: Artesunate reduces but does not prevent posttreatment transmission of Plasmodium falciparum to Anopheles gambiae. J Infect Dis. 2001, 183: 1254-59. 10.1086/319689.View ArticlePubMedGoogle Scholar
- Price RN, Nosten F, Luxemburger C, ter Kuile FO, Paiphun L, Chongsuphajaisiddhi T, White NJ: Effects of artemisinin derivatives on malaria transmissibility. Lancet. 1996, 347: 1654-58. 10.1016/S0140-6736(96)91488-9.View ArticlePubMedGoogle Scholar
- Makanga M, Bassat Q, Falade CO, Premji ZG, Krudsood S, Hunt P, Walter V, Beck HP, Marrast AC, Cousin C, Rosenthal PJ: Efficacy and safety of artemether lumefantrine in the treatment of acute uncomplicated Plasmodium falciparum malaria: a pooled analysis. Am J Trop Med Hyg. 2011, 85: 793-804. 10.4269/ajtmh.2011.11-0069.PubMed CentralView ArticlePubMedGoogle Scholar
- Hamed K, Grueninger H: Coartem®: A decade of patient–centric malaria management. Expert Rev Anti Ther. 2012, 10: 645-659. 10.1586/eri.12.51.View ArticleGoogle Scholar
- Hamel MJ, Adazu K, Obor D, Sewe M, Vulule J, Williamson JM, Slutsker L, Feikin DR, Laserson KF: A reversal in reductions of child mortality in western Kenya, 2003–2009. Am J Trop Med Hyg. 2011, 85: 597-605. 10.4269/ajtmh.2011.10-0678.PubMed CentralView ArticlePubMedGoogle Scholar
- Manyando C, Mkandawire R, Puma L, Sinkala M, Mpabalwani E, Njunju E, Gomes M, Ribeiro I, Walter V, Virtanen M, Schlienger R, Cousin M, Chipimo M, Sullivan FM: Safety of artemether-lumefantrine in pregnant women with malaria: results of a prospective cohort study in Zambia. Malar J. 2010, 9: 249-10.1186/1475-2875-9-249.PubMed CentralView ArticlePubMedGoogle Scholar
- Abdulla S, Sagara I, Borrmann S, D'Alessandro U, González R, Hamel M, Ogutu B, Mårtensson A, Lyimo J, Maiga H, Sasi P, Nahum A, Bassat Q, Juma E, Otieno L, Björkman A, Beck HP, Andriano K, Cousin M, Lefèvre G, Ubben D, Premji Z: Efficacy and safety of artemether-lumefantrine dispersible tablets compared with crushed commercial tablets in African infants and children with uncomplicated malaria: a randomised, single-blind, multicentre trial. Lancet. 2008, 372: 1819-1827. 10.1016/S0140-6736(08)61492-0.View ArticlePubMedGoogle Scholar
- Premji ZG, Abdulla S, Ogutu B, Ndong A, Falade CO, Sagara I, Mulure N, Nwaiwu O, Kokwaro G: The content of African diets is adequate to achieve optimal efficacy with fixed–dose artemether-lumefantrine: a review of the evidence. Malar J. 2008, 7: 244-10.1186/1475-2875-7-244.PubMed CentralView ArticlePubMedGoogle Scholar
- Bukirwa H, Yeka A, Kamya MR, Talisuna A, Banek K, Bakyaita N, Rwakimari JB, Rosenthal PJ, Wabwire-Mangen F, Dorsey G, Staedke SG: Artemisinin combination therapies for treatment of uncomplicated malaria in Uganda. PLoS Clin Trials. 2006, 1: e7-10.1371/journal.pctr.0010007.PubMed CentralView ArticlePubMedGoogle Scholar
- Janssens B, van Herp M, Goubert L, Chan S, Uong S, Nong S, Socheat D, Brockman A, Ashley EA, Van Damme W: A randomized open study to assess the efficacy and tolerability of dihydroartemisinin-piperaquine for the treatment of uncomplicated falciparum malaria in Cambodia. Trop Med Int Health. 2007, 12: 251-259. 10.1111/j.1365-3156.2006.01786.x.View ArticlePubMedGoogle Scholar
- Nambozi M, Van Geertruyden JP, Hachizovu S, Chaponda M, Mukwamataba D, Mulenga M, Ubben D, D’Alessandro U: Safety and efficacy of dihydroartemisinin-piperaquine versus Artemether-lumefantrine in the treatment of uncomplicated Plasmodium falciparum malaria in Zambian children. Malar J. 2011, 10: 50-10.1186/1475-2875-10-50.PubMed CentralView ArticlePubMedGoogle Scholar
- ICH Harmonized Tripartite guideline: Guidelines for Good Clinical practiceE6 (R1). 1996, Current Step 4 version. http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Efficacy/E6_R1/Step4/E6_R1__Guideline.pdfGoogle Scholar
- World Health Organization, Communicable Diseases Cluster: Severe falciparum malaria. Trans R Soc Trop Med Hyg. 2000, 94 (1): S1-90. 10.1016/S0035-9203(00)90413-9.View ArticleGoogle Scholar
- World Health Organization: Assessment and monitoring of antimalarial drug efficacy for the treatment of uncomplicated falciparum malaria. Geneva: WHO, WHO/HTM/RBM/2003.50 2003Google Scholar
- Kamya MR, Yeka A, Bukirwa H, Lugemwa M, Rwakimari JB, Staedke SG, Talisuna AO, Greenhouse B, Nosten F, Rosenthal PJ, Wabwire–Mangen F, Dorsey G: Artemether-lumefantrine versus dihydroartemisinin-piperaquine for treatment of malaria: a randomized trial. PLoS Clin Trials. 2007, 2: e20-10.1371/journal.pctr.0020020.PubMed CentralView ArticlePubMedGoogle Scholar
- Wabwire-Mangen F, Bukirwa H, Rwakimari JB, Staedke SG, Rosenthal PJ, Lugemwa M, Yeka A, Dorsey G, Kamya MR, Talisuna A: Artemether-lumefantrine versus dihydroartemisinin-piperaquine for treating uncomplicated malaria: a randomized trial to guide policy in Uganda. PLoS One. 2008, 3: e2390-10.1371/journal.pone.0002390.PubMed CentralView ArticlePubMedGoogle Scholar
- Ezzet F, van Vugt M, Nosten F, Looareesuwan S, White NJ: Pharmacokinetics and pharmacodynamics of lumefantrine (benflumetol) in acute falciparum malaria. Antimicrob Agents Chemother. 2000, 44: 697-704. 10.1128/AAC.44.3.697-704.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Product monograph. 2010, [http://www.coartem.com/product–monograph.htm] Coartem® –artemether/lumefantrine
- Hung TY, Davis TM, Ilett KF, Karunajeewa H, Hewitt S, Denis MB, Lim C, Socheat D: Population pharmacokinetics of piperaquine in adults and children with uncomplicated falciparum or vivax malaria. Br J Clin Pharmacol. 2004, 57: 253-262.PubMed CentralView ArticlePubMedGoogle Scholar
- Badu K, Afrane YA, Larbi J, Stewart VA, Waitumbi J, Angov E, Ong'echa JM, Perkins DJ, Zhou G, Githeko A, Yan G: Marked variation in MSP-119 antibody responses to malaria in western Kenyan highlands. BMC Infect Dis. 2012, 12: 50-10.1186/1471-2334-12-50.PubMed CentralView ArticlePubMedGoogle Scholar
- Kenya malaria indicator survey (DoMC). 2010,http://measuredhs.com/pubs/pdf/MIS7/MIS7.pdf,
- Elamin SB, Awad AI, Eltayeb IB, Elmardi KA, Hassan AH, Mohamed AO, Malik EM, Mohamad TA: Descriptive study on the efficacy of artemether-lumefantrine in the treatment of uncomplicated Plasmodium falciparum malaria in Sudan. Eur J Clin Pharmacol. 2010, 66: 231-237. 10.1007/s00228-009-0750-4.View ArticlePubMedGoogle Scholar
- Juma EA, Obonyo CO, Akhwale WS, Ogutu BR: A randomized, open–label, comparative efficacy trial of Artemether-lumefantrine suspension versus Artemether-lumefantrine tablets for treatment of uncomplicated Plasmodium falciparum malaria in children in western Kenya. Malar J. 2008, 7: 262-10.1186/1475-2875-7-262.PubMed CentralView ArticlePubMedGoogle Scholar
- Resistance to artemisinin derivatives along the Thai–Cambodian border. Wkly Epidemiol Rec. 2007, 82: 360-Google Scholar
- WHO: The status of drug–resistant malaria along the Thailand–Myanmar border.http://www.who.int/malaria/publications/atoz/drug_resistance_myanmar_thailand_border_may_2012.pdf,
- Arinaitwe E, Sandison TG, Wanzira H, Kakuru A, Homsy J, Kalamya J, Kamya MR, Vora N, Greenhouse B, Rosenthal PJ, Tappero J, Dorsey G: Artemether-lumefantrine versus dihydroartemisinin-piperaquine for falciparum malaria: a longitudinal, randomized trial in young Ugandan children. Clin Infect Dis. 2009, 49: 1629-1637. 10.1086/647946.View ArticlePubMedGoogle Scholar
- Ratcliff A, Siswantoro H, Kenangalem E, Maristela R, Wuwung RM, Laihad F, Ebsworth EP, Anstey NM, Tjitra E, Price RN: Two fixed–dose artemisinin combinations for drug–resistant falciparum and vivax malaria in Papua, Indonesia: an open–label randomised comparison. Lancet. 2007, 369: 757-765. 10.1016/S0140-6736(07)60160-3.PubMed CentralView ArticlePubMedGoogle Scholar
- Bassat Q, Mulenga M, Tinto H, Piola P, Borrmann S, Menéndez C, Nambozi M, Valéa I, Nabasumba C, Sasi P, Bacchieri A, Corsi M, Ubben D, Talisuna A, D'Alessandro U: Dihydroartemisinin-piperaquine and artemether-lumefantrine for treating uncomplicated malaria in African children: a randomised, non-inferiority trial. PLoS One. 2009, 4: e7871-10.1371/journal.pone.0007871.PubMed CentralView ArticlePubMedGoogle Scholar
- Zwang J, Ashley EA, Karema C, D'Alessandro U, Smithuis F, Dorsey G, Janssens B, Mayxay M, Newton P, Singhasivanon P, Stepniewska K, White NJ, Nosten F: Safety and efficacy of dihydroartemisinin-piperaquine in falciparum malaria: a prospective multi-centre individual patient data analysis. PLoS One. 2009, 4: e6358-10.1371/journal.pone.0006358.PubMed CentralView ArticlePubMedGoogle Scholar
- Myint HY, Ashley EA, Day NP, Nosten F, White NJ: Efficacy and safety of dihydroartemisinin-piperaquine. Trans R Soc Trop Med Hyg. 2007, 101: 858-866. 10.1016/j.trstmh.2007.05.018.View ArticlePubMedGoogle Scholar
- Abdulla S, Sagara I: Dispersible formulation of artemether/lumefantrine: specifically developed for infants and young children. Malar J. 2009, 8 (1): S7-10.1186/1475-2875-8-7.PubMed CentralView ArticlePubMedGoogle Scholar
- Makanga M, Krudsood S: The clinical efficacy of artemether/lumefantrine (Coartem). Malar J. 2009, 8 (1): S5-10.1186/1475-2875-8-5.PubMed CentralView ArticlePubMedGoogle Scholar
- Atemnkeng MA, De Cock K, Plaizier-Vercammen J: Quality control of active ingredients in artemisinin-derivative antimalarials within Kenya and DR Congo. Trop Med Int Health. 2007, 12: 68-74.PubMedGoogle Scholar
- Atemnkeng MA, De Cock K, Plaizier-Vercammen J: Post-marketing assessment of content and efficacy of preservatives in artemisinin-derived antimalarial dry suspensions for paediatric use. Malar J. 2007, 6: 12-10.1186/1475-2875-6-12.PubMed CentralView ArticlePubMedGoogle Scholar
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