Anopheles mortality is both age- and Plasmodium-density dependent: implications for malaria transmission
© Dawes et al; licensee BioMed Central Ltd. 2009
Received: 30 July 2009
Accepted: 12 October 2009
Published: 12 October 2009
Daily mortality is an important determinant of a vector's ability to transmit pathogens. Original simplifying assumptions in malaria transmission models presume vector mortality is independent of age, infection status and parasite load. Previous studies illustrate conflicting evidence as to the importance of Plasmodium-induced vector mortality, but very few studies to date have considered the effect of infection density on mosquito survival.
A series of three experiments were conducted, each consisting of four cages of 400-1,000 Anopheles stephensi mosquitoes fed on blood infected with different Plasmodium berghei ookinete densities per microlitre of blood. Twice daily the numbers of dead mosquitoes in each group were recorded, and on alternate days a sample of live mosquitoes from each group were dissected to determine parasite density in both midgut and salivary glands.
Survival analyses indicate that mosquito mortality is both age- and infection intensity-dependent. Mosquitoes experienced an initially high, partly feeding-associated, mortality rate, which declined to a minimum before increasing with mosquito age and parasite intake. As a result, the life expectancy of a mosquito is shown to be dependent on both insect age and the density of Plasmodium infection.
These results contribute to understanding in greater detail the processes that influence sporogony in the mosquito, indicate the impact that parasite density could have on malaria transmission dynamics, and have implications for the design, development, and evaluation of transmission-blocking strategies.
Daily mortality is the most important determinant of a mosquito's ability to transmit pathogens, influencing the probability to encounter infectious hosts, survive the extrinsic incubation period and transmit the infection . The period necessary for the parasite to reach its infective stage within the vector often takes an appreciable portion of the vector's life-span and, therefore, only a small proportion actually survive long enough in nature to transmit the infection. As a result, the basic reproduction number (R0) of vector-borne infections is critically dependent on the life-span of the vector, and in particular on the infective life expectancy [2, 3]. Small changes in the daily mortality rate can result in relatively large changes in transmission. In support of this, Macdonald's malaria models indicated that at equilibrium, the weakest link in the chain of transmission was the survivorship of the adult female Anopheles, providing a rationale for a DDT-focused, World Health Organization-coordinated eradication campaign that successfully eliminated malaria transmission among approximately 700 million people . Therefore, understanding the determinants of mosquito survival can have important implications for the design and assessment of new malaria control strategies.
Original simplifying assumptions in malaria transmission models include that vector mortality is independent of and, therefore, unaffected by, mosquito age, infection status and parasite load [1, 3, 5–7]. This has resulted in estimates of the daily survival rate entering as constants in mathematical equations of epidemiological indices such as the vectorial capacity and the entomological inoculation rate, in models of population dynamics and in the assessment of control strategies. These assumptions have continued to permeate malaria transmission models despite conflicting evidence as to their validity.
The assumption of mosquito mortality being independent of age was first articulated by Macdonald, who reasoned that environmental insults, disease, and predation would kill mosquitoes before they died of old age . Macdonald, therefore, based his mathematical treatment of survival on the factor p, the probability of a mosquito surviving from one day to the next. Some studies support this notion [8, 9] whereas others have found evidence of mosquito senescence, particularly in laboratory populations [10–16]. Notably, Clements and Patterson  re-analysed published reports of mosquito mortality and concluded that many species exhibit age-dependent mortality, with most, but not all, consistent with the Gompertz model . More recently, Styer et al found that mortality was highly age-dependent in both sexes of Aedes aegypti, and that the age at which a mosquito first bites an infectious host is an important indicator of the probability of transmitting a pathogen.
Despite these studies clearly calling into question the assumption of no senescence in mosquito populations, the common operational assumption remains that insect vector mortality is independent of age, and this has been incorporated into many mathematical models [7, 19–24]. The reluctance for this to change can primarily be ascribed to the fact that allowing mortality to be constant with age leads to the exponential model for the distribution of survival times, which has the significant advantage of mathematical simplicity and tractability, and reduces the number and complexity of variables that need to be considered. However, acceptance of this non-senescence assumption leads to the simplified view that the potential of mosquitoes to survive and transmit disease is constant regardless of their age, and it has been shown that quantitative models that assume non-senescence can produce results with substantial errors .
It has been argued that there will be strong selection pressure on Plasmodium not to reduce vector survival, as both partners benefit from high rates of survival and of blood-feeding; the mosquito to increase its reproductive success and the parasite to ensure its transmission . However, investigations into the pathogenicity of malarial parasites in mosquitoes have not been conclusive, resulting in conflicting evidence as to whether malaria parasites are benign to their vectors. Laboratory studies are contradictory; some indicate that the survival rate of infected mosquitoes is not different from that of non-infected mosquitoes [26–31], whereas others indicate reduced survival [32–37]. Ferguson and Read  conducted a meta-analysis of 22 previously published laboratory studies, and concluded that overall, malaria parasites do reduce mosquito survival, but stated that these mortality effects were more likely to be detected in vector-parasite combinations not occurring naturally in the field and in studies of longer duration. Field studies which have explored parasite-induced vector mortality indirectly, have also yielded conflicting results; some supporting [39, 40] and others not supporting  its operation.
It has also been suggested that malaria parasites may only be harmful to mosquitoes when parasite burdens are exceedingly high [28, 35], which has been used to refute the existence of Plasmodium-induced mortality in nature, as most naturally infected mosquitoes carry, on average, only two to three oocysts of Plasmodium falciparum[42–45]. However, the absence of high oocyst burdens in population samples could also be due to the mortality of more heavily infected mosquitoes . Very few studies to date have explicitly and systematically considered the effect of infection density on mosquito mortality, and those which have, have not reported consistent results. Whilst some authors suggest that mosquito survivorship is not negatively correlated with parasite density [29, 32], others found that mosquito mortality increased with oocyst burden [35–37, 46]. The review by Ferguson and Read  concluded that there is no relationship between mortality and mean oocyst burden in the five studies that reported oocyst burden, but suggested that sporozoite load may be the prime determinant of mosquito mortality as mortality differences only became apparent in studies of longer duration when sporozoites would be in the salivary glands.
In the context of the renewed global efforts to eliminate malaria, it has become increasingly important to obtain a better understanding of the component of the malaria life cycle taking place within the mosquito. Using the Plasmodium berghei-Anopheles stephensi experimental system, it has been rigorously demonstrated that parasite development during sporogony is density-dependent . Using the same system, this paper investigates the validity of the original simplifying assumptions that mosquito mortality is independent of age, infection status, and infection density, which are commonly used in the formulation of mathematical models of malaria transmission. The ultimate aim is that of generating testable hypotheses that serve to prompt investigation of whether similar phenomena apply to any of the complex, numerous, and multifarious parasite-vector combinations that play a role in malaria transmission in the field.
Summary statistics for cages of An. stephensi mosquitoes fed different estimated P. berghei ookinete densities
Estimated ookinete density fed (per μl)
Mean‡ number of oocysts per mosquito on day 10* (range)
Mean‡ sporozoite score† per mosquito on day 22*
Total number of mosquitoes in each cage
Median survival (days)
32 (30, 34)
ND (25, ND)
26 (23, 31)
21 (19, 24)
33 (32, 35)
ND (ND, ND)
34 (32, ND)
30 (29, ND)
42 (41, ND)
36 (35, 37)
36 (34, 38)
34 (32, 35)
The populations of mosquitoes were followed over time post-feeding (which is also a proxy for mosquito age in this experiment) by recording the number of dead females in each group twice each day. In addition, for the first six days, and on alternate days after this time, a sample of twenty live mosquitoes from each group (with the exception of the control) were dissected to remove both the midgut and (from approximately day 10 onwards) salivary glands to determine parasite density in both midgut and salivary glands using fluorescence microscopy. This paper reports the results of the survival analysis. A summary of the resulting dynamics of parasite stages and densities with time post-feeding has been presented elsewhere .
Survival functions for each of the four groups (based on the ookinete density fed to the mosquitoes) in each of the three experiments, were estimated using the Kaplan-Meier estimate , classifying those mosquitoes lost to follow-up, e.g. those which were killed for dissection, as censored observations. (For details on the calculation of the Kaplan-Meier estimate see additional file 1: 'Detailed statistical methods'.)
The median survival time (with 95% confidence intervals) was calculated for each group to compare survival times, by determining the time beyond which 50% of the individuals in the population are expected to survive. The Mantel-Cox test and a log-rank test for trend were used to compare the survival distributions of the four groups within each of the experiments. The Mantel-Cox test is used for two-sample comparisons and is based on a test statistic with a chi-squared distribution and one degree of freedom under the null hypothesis that there is no difference between the survivorship of the individuals in the two groups under comparison [52, 53] (for further details see additional file 1: 'Detailed statistical methods'). The log-rank test for trend was computed because the four groups to be compared in each experiment represented ordered, increasing, densities of infection. Therefore, the codes assigned to each of the mosquito groups were the number of ookinetes per μl of blood fed, which allowed this test to investigate if a linear trend exists between parasite density and survival. The resulting test statistic has a chi-squared distribution with one degree of freedom, under the null hypothesis of no trend across the groups [52, 54] (for further details see additional file 1: 'Detailed statistical methods').
The combined datasets from the three experiments were analysed using Cox regression survival analysis (proportional hazards model) fitting ookinete density fed to the mosquitoes first as a categorical variable to test for differences in survival between cages, and subsequently as a continuous variable to explore the impact of an increase in parasite density on mosquito survival. This statistical analysis allows the impact of parasite density on mosquito survival to be tested whilst controlling for variation due to experiment.
Estimation of mosquito mortality rates
The modelling of survival data centers on the hazard function (the instantaneous death rate), which is used to express the risk or hazard of death at time t. Kaplan-Meier estimates, which assume that this hazard function is constant between successive death times, were calculated and plotted for the mid-point of each time-interval (for details as to their calculation see additional file 1: 'Detailed statistical methods').
where ν0, δ0 and θ0 represent the baseline hazard experienced by uninfected mosquitoes, and ν1, δ1 and θ1 represent the additional mortality per unit increase in ookinete density. Equation (2) was fitted to the full dataset using non-linear least squares estimation, and allowing the average mosquito mortality rate to vary between experiments to account for inter-experimental variability. Analysis of variance tests were conducted on nested versions of this full model to find the most parsimonious hazard function using the 'nls' and 'anova' commands in the statistical package R  as described by Bolker . Ninety-five percent confidence intervals (95% C.I.) for the best-fit model were estimated using bootstrapping methods (see additional file 2: 'Generation of 95% confidence intervals for the best-fit model'). The survivorship function contains the integrated hazard function as detailed in additional file 1: 'Detailed statistical methods'.
Equation (4) was evaluated using the Berkeley Madonna numerical integration package (Version 8.0.1)  to calculate how the life expectancy of mosquitoes varied with the number of fed ookinetes and time post-engorgement. In the literature, mosquito life-span has been previously discussed in relation to oocyst rather than ookinete density, and therefore by way of illustration, mosquito life expectancy was also related to the mean oocyst load found in the sample of mosquitoes dissected from each of the cages 10 days post-bloodfeed as described in additional file 3: 'Calculating how life expectancy of mosquitoes varies with mean oocyst density on day 10 and time post-engorgement'.
Table 1 summarizes the data from each of the three experiments (approximately 400-1,000 female mosquitoes fed in each of the cages). The median survival time experienced by each of the cages of mosquitoes within each experiment shows a general trend towards a decrease in survival with an increase in average parasite load.
Results of two-sample and multi-sample statistical comparisons of An. stephensi survival times.
Mosquito Group (Ookinete density per μl of blood)
Hazard Ratio (HR)
All 4 Groups
All 4 Groups
All 4 Groups
Cox regression analysis results.
Hazard Ratio (95% C.I.)
Ookinete density per μl of blood fed as a categorical variable
2.125 (1.707, 2.645)
0.928 (0.784, 1.099
2.153 (1.737, 2.669)
1.369 (1.200, 1.561)
2.429 (1.974, 2.988)
1.894 (1.649, 2.176)
0.663 (0.598, 0.735)
0.368 (0.299, 0.452)
Ookinete density per μl of blood fed as a continuous variable
3.26 × 10-4
2.88 × 10-5
1.00032 (1.0003, 1.0004)
0.656 (0.593, 0.727)
0.622 (0.569, 0.680)
Allowing mortality rates to vary between the different experiments significantly improved the fit of the model to the observed data; mortality rates in the second and third experiments were on average 30% (95% C.I., 17-42%) and 32% (21-42%) lower than in experiment 1 respectively, suggesting substantial between-experiment variability.
The role of model systems
Any single model system cannot accurately reflect the biology of all natural parasite-vector combinations. Nonetheless studies on the biology of Plasmodium spp per se, and their interactions with Anopheles mosquitoes have been advanced considerably by the analysis of P. berghei in An. stephensi. This paper exploits the unique ability to study the effect of increasing densities of homogeneous populations of P. berghei (clone) on the survival of An. stephensi (inbred iso-female line) in a controlled biological environment. The results reported here indicate that, in captivity, An. stephensi mosquitoes experience initial blood feed-associated and age-dependent mortalities, and that their survival decreases with the intensity of P. berghei infection.
Blood feeding- and age-dependent mortality
The time (age)-dependent curves of survivorship (Figure 2) and mortality rates (Figure 3) indicate that female mosquitoes have the potential to senesce, in agreement with previously published studies in a variety of species [10, 11, 16, 17, 59]. Most previous analyses have used the Gompertz hazard function. However, this was not adequate to describe the mortality rates experienced by the mosquitoes in the experiments presented here; in each of the mosquito groups, including the control, mortality rates were found to be high immediately after feeding, decreasing initially to a minimum before increasing with age. This functional form, depicted in Figure 3, which describes the mortality rates experienced by the mosquitoes, could result from a number of biological processes. The initial mortality (measured by parameter θ of the hazard function in Equation (1) of the Methods section) is likely to be in part associated with the act of feeding itself, for example, allowing the bacterial population within the mosquito midgut to proliferate . Additionally, this early mortality in the control group might also be attributed to asexual stages of the parasite, or parasite-induced factors present in the mouse blood up-regulating the mosquito's immune system , which could be costly to the survival of the mosquito. After reaching a minimum mortality rate at an intermediate time post-engorgement, the increase in mortality rate (measured primarily by parameter ν of the hazard function in Equation (1)) is expected to represent the effect of mosquito ageing.
These results suggest that the age at which a mosquito bites an infectious host is important in determining the probability that it will transmit the parasite and contribute to malaria transmission. Mosquitoes exhibiting age-dependent mortality patterns are more likely to transmit pathogens if they bite an infectious host when their mortality rate is at a minimum, as they are more likely to survive the extrinsic incubation period. Clements and Patterson  and Styer et al illustrated the importance of accepting this concept of mosquito senescence, showing that the longevity factor  and the vectorial capacity for a variety of mosquito species can be significantly overestimated if calculated using the simpler exponential hazard model compared to a hazard model which is age-dependent such as the Gompertz model. Consequently, the potential impact of anti-vectorial control measures could be underestimated by assuming age-independent mosquito mortality. Gillies  even called for the exponential hazard model that assumes no senescence to be 'buried', as it produces results which are 'at best approximations'. Recent studies that explore the potential impact of novel control strategies such as fungal biopesticide sprays have acknowledged this by using a mosquito age-structured model and adult female age (time since infection)-dependent mortality .
The study of mosquito cohorts in the laboratory presented here provides patterns of mortality and survival under conditions in which many individuals may survive until old age, and therefore represent the baseline state which is inevitably modified on exposure to natural conditions. It is particularly important to determine whether age-dependent mortality is relevant in field situations as it has previously been accepted that few organisms die of senescence in nature, with the majority being killed by other hazards such as predators or disease before they reach 'old age' . Previous research has found both constant survival rates in natural settings in An. gambiae (using Polovodova age-grading) [9, 41], and increasing death rates with insect age in many mosquito species in the field [12, 17, 59]. A conceptual shift from age-independent to age-dependent mortality rates and an understanding of their relative merits in natural malaria transmission settings requires detailed knowledge of mosquito population age structure and its relation to pathogen transmission dynamics. Current age-grading techniques used in the field are most commonly based on morphological changes in the mosquito, such as the detection of tracheal skeins, which only permits differentiation between nulliparous and parous females , or the enumeration of follicular relics for the assessment of physiological age , which is difficult to implement in the field (requiring training and the use of phase-contrast microscopy), as discussed by Hugo et al, 2008 . There is therefore a need for the development of novel age-grading assays that allow investigation of the age structure in mosquito populations prior to and after interventions. Additionally, other age-related changes also occur in mosquitoes, such as changes in flight performance , structure of the salivary glands , immune function [70, 71], and efficiency of detoxification mechanisms [72, 73], showing that mosquitoes, like other organisms, experience age-related structural and functional deterioration.
Ferguson and Read's review  illustrated the inconsistent results from research aiming to elucidate the impact of Plasmodium infection on mosquito mortality. This review also indicated a lack of systematic research to understand the effect of increasing parasite density on mosquito survival, despite it often being postulated that Plasmodium is only harmful to the vector when parasite loads are very high [28, 35]. The results presented here indicate that mosquito mortality was influenced by the range of intensities of Plasmodium infection explored. In general, the higher the parasite density fed to the mosquitoes the greater the mortality experienced as indicated by the Cox regression results (Table 3). In the first two experiments, mosquito survival in the group with the lowest Plasmodium density (100 ookinetes/μl of blood; see Table 1 for resulting oocyst and sporozoite densities) was found either not to differ from, or to be lower than, that in the control group, whereas significant differences were found with higher parasite densities, in a dose-dependent manner. This appears to be consistent with the few previous studies that have considered infection density [33, 37, 46, 74]. In particular, Klein et al found that the survival rates of Anopheles dirus with less than 10 Plasmodium cynomolgi oocysts were not significantly different from those in uninfected mosquitoes, whereas the mean survival rates of the groups infected with over 41 oocysts per mosquito were significantly lower. Interestingly, the results from the third experiment in this paper indicated that even very low parasite densities (50 ookinetes per μl of blood fed, which (from Table 1) resulted in a mean oocyst load of 11 per mosquito on day 10) can reduce mosquito survival. The fitness cost to mosquitoes of feeding on infected versus uninfected hosts may even be higher than estimated in this experimental design, as the control group was fed on blood with asexual parasitaemia, which by potentially eliciting costly immune responses, could result in increased mosquito mortality .
The impact of parasite density on mosquito mortality is further exemplified by the relationship between the parameter values of the empirical hazard function describing how mortality rates change over time (Equation (2) in the Methods section) and parasite density fed to each of the mosquito groups (Figure 4). Parameter θ (the intercept), which describes the rate of mortality immediately post-feeding, varies with parasite density, being lowest in uninfected mosquitoes and increasing with parasite density fed to the mosquito. This indicates that this initial mortality may not only be associated with bacteria proliferating in the midgut as discussed above, but that there is also an impact of, or interaction with, Plasmodium infection, potentially due to rupture of the midgut causing septic injury, particularly at high parasite densities when the ability of the midgut to repair and seal [75, 76] may be compromised (as seen in Figure 3 of ). Parameter δ, which primarily measures the degree of the subsequent decline in the mortality rate, decreases with parasite density, indicating a steeper decrease in mortality with increasing parasite density (due to starting from a higher intercept). Finally, parameter ν, which measures predominantly the slope of the final rise in mortality rate with time post-feeding, is positively and significantly associated with infection density, indicating that mortality not only increases with age, but that the rate of this increase is amplified by the intensity of Plasmodium infection. This suggests that Plasmodium density has the potential of affecting the shape of the hazard function of infected Anopheles mosquitoes over their full life-span. Interestingly, no specific change in mortality rate was seen on day 12 or 14 when sporozoites were first found in the salivary glands of the sample of mosquitoes dissected from each of the groups .
These results reveal that under laboratory conditions, Anopheles mortality is not only influenced by Plasmodium infection, but that this may also be an important source of density dependence in the system. This may, therefore, go someway towards explaining the varied and often conflicting results found in the past and reviewed by Ferguson and Read . The majority of previous experiments had not explored or even reported parasite density, and therefore the density used in each study may explain why some have found evidence for Plasmodium-induced mosquito mortality whilst others have not. Additionally, Plasmodium-density dependent mortality has the potential to explain the low oocyst loads found in the field as those mosquitoes with large numbers of oocysts may have died as a result of infection as well as, or because of an interaction with, environmental factors. Density-dependent, parasite-induced vector mortality has been reported in other vector-borne diseases, and particularly in the filarial parasites, both in captivity [55, 78, 79] and in the field [80, 81].
It is recognized that there are a number of possible mechanisms and stages during Plasmodium development in which infection could damage the vector and therefore increase mortality. There is mixed evidence for many of these potential mechanisms, as discussed below, and it is possible that all could be exacerbated or altered in some way by the density of infection. The parasite can cause physical tissue damage, for example ookinetes perforating the mosquito midgut, and this could also increase susceptibility to bacterial infection and/or invasion by other parasites [82, 83]. However, the 'time bomb' theory suggests that as the parasite passes through the midgut wall it initiates apoptosis and expulsion of the midgut cell, which is accompanied by a sealing of the midgut epithelium which regains integrity and a healthy appearance within 48 hours [75, 76]. As mentioned above, this seal may not be entirely aseptic, allowing bacterial infection to spread, especially during an infection with high parasite numbers when the ability of the midgut to repair may be compromised or slowed. It has also been postulated that Plasmodium infection may lead to resource depletion in the mosquito as levels of amino acids in their haemolymph have been shown to be reduced, and glucose usage has shown to be up to eight times as much as in uninfected mosquitoes [84, 85]. In contrast however, Rivero and Ferguson (2003)  found no evidence of a parasite-associated reduction in the energetic budget of mosquitoes. Additionally, since infection may be associated with a reduction in egg production [34, 35], which is expensive in terms of resources, infection may even result in a saving of nutrients. In addition, mosquitoes have been shown to mount a variety of immune responses to pathogens [87–89], which can be energetically costly, incurring reproductive costs [90–92]. Such costly immune responses may be induced by the Plasmodium infection itself and/or by the increase in gut bacteria due to blood feeding .
The feeding behaviour of infected mosquitoes has also been shown to differ between uninfected and infected mosquitoes, with infected mosquitoes spending more time feeding, probing more regularly, more likely taking multiple blood meals, being more persistent feeders, and having poorer flight ability [94–98]. These additional behavioural changes can increase the mortality of infected mosquitoes whilst feeding in the field , and can vary temporally with the developmental stage of the parasite, balancing opportunities for Plasmodium transmission with the risk of feeding-associated mortality . Laboratory studies have reported that the feeding persistence of female An. stephensi is decreased in the presence of Plasmodium yoelii nigeriensis oocysts, but increased when the malaria has developed into transmissible sporozoites in the salivary glands . Laboratory experiments, such as those reported here, exclude these possible indirect costs of infection such as increased risk of predation, and therefore the effect of infection on mosquito mortality may be more pronounced in the field compared to the laboratory due to greater levels of environmental stress. Insects in this study were fed only once and subsequently kept under controlled laboratory conditions, instead of undergoing their natural gonotrophic cycle of feeding, oviposition, and host-seeking, which may additionally impact on their chances of survival.
The work reported here was carried out using an experimental vector-parasite combination, the model system P. berghei-An. stephensi, which allowed the investigation to be conducted under tightly controlled conditions. The average oocyst numbers resulting from the ookinete densities fed to the mosquitoes in this study are higher than the average number of Plasmodium falciparum oocysts found in Anopheles gambiae in the field, and therefore the density dependence found may not be as evident in studies of vector-parasite combinations found naturally. Additionally, it is recognized that the analysis of vector-parasite combinations not naturally found in the field may increase the chance of finding evidence of Plasmodium-induced vector mortality, as stated by Ferguson and Read . As well as this perhaps resulting from a lack of parasite-vector co-adaptation, it may also be due to the greater likelihood of distinguishing parasite-induced effects from environmental risks under the more controlled conditions of the laboratory. In this study the removal of extraneous variables has permitted the unequivocal identification of density-dependent Plasmodium induced Anopheles mortality, and therefore, as in Sinden et al, the results have generated testable hypotheses, which now should be followed up with studies of other Plasmodium-Anopheles combinations, including the less tractable human malaria parasites and their multiple vector species, both in the laboratory and in the field.
Conclusions and implications for malaria transmission and control
These results indicate that, in the model system investigated, the life expectancy of Anopheles mosquitoes is dependent on both insect age and the density of Plasmodium infection, as depicted in Figure 5. This emphasizes the importance of testing these hypotheses in combinations of medical importance, and of understanding the impact of these factors on mosquito mortality, as they influence the probability of a mosquito surviving the extrinsic incubation period and contributing to malaria transmission. Linking these results to previous findings  (which illustrated density-dependent transitions between sporogony parasite stages), indicates that intermediate 'optimum' parasite densities may exist for the parasite to complete transmission, and it is likely that these optima will depend on the specific Plasmodium-Anopheles combination. Understanding such intricacies is of utmost importance, as it is possible that interventions could have unexpected outcomes; reducing high parasite load for example, could inadvertently increase the life expectancy of the vector and relax the density-dependent constraints operating upon sporogony within the vector, facilitating successful transmission of the pathogen. As a result it is important for studies of transmission-blocking strategies to report efficacy in terms of reductions in prevalence as well as parasite density to facilitate understanding of the impact of such interventions on malaria transmission.
As vector mortality is a particularly sensitive component of pathogen transmission, quantitative models seeking to describe transmission dynamics within the vector that do not include these processes could produce misleading results or miss epidemiologically important outcomes. The results presented here suggest that high parasite loads have the potential to reduce vector competence (summarized as the per capita probability of an ingested gametocyte to generate infectiousness) and vectorial capacity (which includes the daily probability of vector survival and the expectation of infective life or 'longevity factor'). Age- and parasite density-dependent mosquito mortality, as well as density-dependent Plasmodium development are in the process of being included into mathematical models that will provide a more comprehensive description of the processes that influence sporogony in the mosquito and the expectation of infective life. The usefulness of such models for the design, development, and evaluation of transmission-blocking strategies will be reported elsewhere.
We would like to thank Chandra Ramakrishnan for contributions to the design and supervision of the first experiment, Kalpana Lal for assistance and technical help during the second and third experiments, and Ken Baker for technical help providing mosquitoes.
This work has been supported by the Medical Research Council, UK (EJD and M-GB), the EU RTN networks and EU BioMalPar programmes (RES), and the European Commission FP7 Collaborative project HEALTH-F3-2008-223736 (TSC and M-GB).
- Macdonald G: The epidemiology and control of malaria. 1957, London: Oxford University PressGoogle Scholar
- Dye C: The analysis of parasite transmission by bloodsucking insects. Ann Rev Entomol. 1992, 37: 1-19. 10.1146/annurev.en.37.010192.000245.View ArticleGoogle Scholar
- Garrett-Jones C: Prognosis for interruption of malaria transmission through assessment of the mosquito's vectorial capacity. Nature. 1964, 204: 1173-1175. 10.1038/2041173a0.View ArticlePubMedGoogle Scholar
- World Health Organization for Africa: Implementation of indoor residual spraying of insecticides for malaria control in the WHO African region report. 2007, Geneva: World Health OrganizationGoogle Scholar
- Macdonald G: The analysis of equilibrium in malaria. Trop Dis Bull. 1952, 8: 33-36.Google Scholar
- Molineaux L, Dietz K, Thomas A: Further epidemiological evaluation of a malaria model. Bull World Health Organ. 1978, 56: 565-571.PubMed CentralPubMedGoogle Scholar
- Molineaux L, Gramiccia G: The Garki Project. Research on the Epidemiology and Control of Malaria in the Sudan Savanna of West Africa. 1980, Geneva: World Health OrganizationGoogle Scholar
- Keener GG: Detailed observations on the life history of Anopheles quadrimaculatus. J Natl Malaria Soc. 1945, 4: 263-270.Google Scholar
- Russell PF, Rao TR: Observations on longevity of Anopheles culicifacies imagines. Am J Trop Med. 1942, 22: 517-533.Google Scholar
- Kershaw WE, Lavoipierre MMJ, Chalmers TA: Studies on the intake of microfilariae by their insect vectors, their survival and their effect on the survival of their vectors. I: Dirofilaria immitis and Aedes aegypti. Ann Trop Med Parasitol. 1953, 47: 207-224.PubMedGoogle Scholar
- Kershaw WE, Chalmers TA, Lavoipierre MMJ: Studies on arthropod survival. I: The pattern of mosquito survival in laboratory conditions. Ann Trop Med Parasitol. 1954, 48: 442-450.PubMedGoogle Scholar
- Gillies MT, Wilkes TJ: A study of the age-composition of population of Anopheles gambiae Giles and An. funestus Giles in North-Eastern Tanzania. Bull Entomol Res. 1965, 56: 237-262. 10.1017/S0007485300056339.View ArticlePubMedGoogle Scholar
- Briegel H, Kaiser C: Life span of mosquitoes (Culicidae, Diptera) under laboratory conditions. Gerontologia. 1973, 19: 240-249. 10.1159/000211976.View ArticlePubMedGoogle Scholar
- Harrington LC, Buonaccorsi JP, Edman JD, Costero A, Kittayapong P, Clark GG, Scott TW: Analysis of survival of young and old Aedes aegypti (Diptera: Culicidae) from Puerto Rico and Thailand. J Med Entomol. 2001, 38: 537-547.View ArticlePubMedGoogle Scholar
- Okech BA, Gouagna LC, Killeen GF, Knols BG, Kabiru EW, Beier JC, Yan G, Githure JI: Influence of sugar availability and indoor microclimate on survival of Anopheles gambiae (Diptera: Culicidae) under semifield conditions in Western Kenya. J Med Entomol. 2003, 40: 657-663.View ArticlePubMedGoogle Scholar
- Styer LM, Carey JR, Wang J-L, Scott TW: Mosquitoes do senesce: departure from the paradigm of constant mortality. Am J Trop Med Hyg. 2007, 76: 111-117.PubMed CentralPubMedGoogle Scholar
- Clements AN, Paterson GD: The analysis of mortality and survival rates in wild populations of mosquitoes. J Applied Ecology. 1981, 18: 373-399. 10.2307/2402401.View ArticleGoogle Scholar
- Gompertz B: On the nature of the function expressive of the law of human mortality, and on a new mode of determining the value of life contingencies. Phil Trans R Soc Lond. 1825, 115: 513-583. 10.1098/rstl.1825.0026.View ArticleGoogle Scholar
- Bailey NTJ: The Biomathematics of Malaria. 1982, London: Charles Griffin & CoGoogle Scholar
- Dietz K, Molineaux L, Thomas A: A malaria model tested in the African savannah. Bull World Health Organ. 1974, 50: 347-357.PubMed CentralPubMedGoogle Scholar
- Garrett-Jones C, Shidrawi GR: Malaria vectorial capacity of a population of Anopheles gambiae. An exercise in epidemiological entomology. Bull World Health Organ. 1969, 40: 531-545.PubMed CentralPubMedGoogle Scholar
- Ishikawa H, Ishii A, Nagai N, Ohmae H, Harada M, Suguri S, Leafasia J: A mathematical model for the transmission of Plasmodium vivax malaria. Parasitol Int. 2003, 52: 81-93. 10.1016/S1383-5769(02)00084-3.View ArticlePubMedGoogle Scholar
- Killeen GF, McKenzie FE, Foy BD, Schieffelin C, Billingsley PF, Beier JC: A simplified model for predicting malaria entomologic inoculation rates based on entomologic and parasitologic parameters relevant to control. Am J Trop Med Hyg. 2000, 62: 535-544.PubMed CentralPubMedGoogle Scholar
- Torres-Sorando LJ, Rodríguez DJ: Models of spatio-temporal dynamics in malaria. Ecol Modelling. 1997, 104: 231-240. 10.1016/S0304-3800(97)00135-X.View ArticleGoogle Scholar
- Schwartz AS, Koella JC: Trade-offs, conflicts of interest and manipulation in Plasmodium-mosquito interactions. Trends Parasitol. 2001, 17: 189-194. 10.1016/S1471-4922(00)01945-0.View ArticlePubMedGoogle Scholar
- Boyd MF: On the correlation between the incidence of stomach and gland infection in Anopheles quadrimaculatus infected with Plasmodium vivax. Am J Trop Med. 1940, 20: 129-131.Google Scholar
- Chege GMM, Beier JC: Effect of Plasmodium falciparum on the survival of naturally infected Afrotropical Anopheles (Diptera: Culicidae). J Med Entomol. 1990, 27: 454-458.View ArticlePubMedGoogle Scholar
- De Buck A: Some results of six years' mosquito infection work. Am J Hyg. 1936, 24: 1-18.Google Scholar
- Gamage-Mendis AC, Rajakaruna J, Weerasinghe S, Mendis C, Carter R, Mendis KN: Infectivity of Plasmodium vivax and P. falciparum to Anopheles tessellatus; relationship between oocyst and sporozoite development. Trans R Soc Trop Med Hyg. 1993, 87: 3-6. 10.1016/0035-9203(93)90396-8.View ArticlePubMedGoogle Scholar
- Hogg JC, Hurd H: The effects of natural Plasmodium falciparum infection on the fecundity and mortality of Anopheles gambiae s.l. in north east Tanzania. Parasitology. 1997, 114: 325-331. 10.1017/S0031182096008542.View ArticlePubMedGoogle Scholar
- Robert V, Verhave JP, Carnevale P: Plasmodium falciparum infection does not increase the precocious mortality rate of Anopheles gambiae. Trans R Soc Trop Med Hyg. 1990, 84: 346-347. 10.1016/0035-9203(90)90309-3.View ArticlePubMedGoogle Scholar
- Ferguson HM, Read AF: Genetic and environmental determinants of malaria parasite virulence in mosquitoes. Proc R Soc Lond B. 2002, 269: 1217-1224. 10.1098/rspb.2002.2023.View ArticleGoogle Scholar
- Gad AM, Maier WA, Piekarski G: Pathology of Anopheles stephensi after infection with Plasmodium berghei berghei. Z Parasitenkd. 1979, 60: 249-261. 10.1007/BF00929172.View ArticlePubMedGoogle Scholar
- Hogg JC, Hurd H: Malaria-induced reduction of fecundity during the first gonotrophic cycle of Anopheles stephensi mosquitoes. Med Vet Entomol. 1995, 9: 176-180. 10.1111/j.1365-2915.1995.tb00175.x.View ArticlePubMedGoogle Scholar
- Hogg JC, Hurd H: Plasmodium yoelli nigeriensis: the effect of high and low intensity upon the egg production and bloodmeal size of Anopheles stephensi during three gonotrophic cycles. Parasitology. 1995, 111: 555-562. 10.1017/S0031182000077027.View ArticlePubMedGoogle Scholar
- Klein TA, Harrison BA, Andre RG, Whitmire RE, Inlao I: Detrimental effects of Plasmodium cynomolgi infections on the longevity of Anopheles dirus. Mosq News. 1982, 42: 265-271.Google Scholar
- Klein TA, Harrison BA, Grove JS, Dixon SV, Andre RG: Correlation of survival rates of Anopheles dirus A (Diptera: Culicidae) with different infection densities of Plasmodium cynomolgi. Bull World Health Organ. 1986, 64: 901-907.PubMed CentralPubMedGoogle Scholar
- Ferguson HM, Read AF: Why is the effect of malaria parasites on mosquito survival still unresolved?. Trends Parasitol. 2002, 18: 256-261. 10.1016/S1471-4922(02)02281-X.View ArticlePubMedGoogle Scholar
- Anderson RA, Knols BGJ, Koella JC: Plasmodium falciparum sporozoites increase feeding-associated mortality of their mosquito hosts Anopheles gambiae s.l. Parasitology. 2000, 120: 329-333. 10.1017/S0031182099005570.View ArticlePubMedGoogle Scholar
- Lyimo EO, Koella JC: Relationship between body size of adult Anopheles gambiae s.l. and infection with the malaria parasite Plasmodium falciparum. Parasitology. 1992, 104: 233-237. 10.1017/S0031182000061667.View ArticlePubMedGoogle Scholar
- Lines JD, Wilkes TJ, Lyimo EO: Human malaria infectiousness measured by age-specific sporozoite rates in Anopheles gambiae in Tanzania. Parasitology. 1991, 102: 167-177. 10.1017/S0031182000062454.View ArticlePubMedGoogle Scholar
- Pringle G: A quantitative study of naturally-acquired malaria infections in Anopheles gambiae and Anopheles funestus in a highly malarious area of East Africa. Trans R Soc Trop Med Hyg. 1966, 60: 626-632. 10.1016/0035-9203(66)90009-5.View ArticlePubMedGoogle Scholar
- Haji H, Smith T, Charlwood JT, Meuwissen JH: Absence of relationships between selected human factors and natural infectivity of Plasmodium falciparum to mosquitoes in an area of high transmission. Parasitology. 1996, 113: 425-431. 10.1017/S0031182000081488.View ArticlePubMedGoogle Scholar
- Graves PM, Burkot TR, Carter R, Cattani JA, Lagog M, Parker J, Brabin BJ, Gibson FD, Bradley DJ, Alpers MP: Measurement of malarial infectivity of human populations to mosquitoes in the Madang area, Papua, New Guinea. Parasitology. 1988, 96: 251-263. 10.1017/S003118200005825X.View ArticlePubMedGoogle Scholar
- Rosenberg R, Andre RG, Somchit L: Highly efficient dry season transmission of malaria in Thailand. Trans R Soc Trop Med Hyg. 1990, 84: 22-28. 10.1016/0035-9203(90)90367-N.View ArticlePubMedGoogle Scholar
- Ferguson HM, Mackinnon MJ, Chad BH, Read AF: Mosquito mortality and the evolution of malaria virulence. Evolution. 2003, 57: 2792-2804.View ArticlePubMedGoogle Scholar
- Sinden RE, Dawes EJ, Alavi Y, Waldock J, Finney O, Mendoza J, Butcher GA, Andrews L, Hill AV, Gilbert SC, Basáñez MG: Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. PLoS Pathog. 2007, 3: e195-10.1371/journal.ppat.0030195.PubMed CentralView ArticlePubMedGoogle Scholar
- Sinden RE, Butcher GA, Billker O, Fleck SL: Regulation of infectivity of Plasmodium to the mosquito vector. Adv Parasitol. 1996, 38: 53-117. 10.1016/S0065-308X(08)60033-0.View ArticlePubMedGoogle Scholar
- Franke-Fayard B, Trueman H, Ramesar J, Mendoza J, Keur van der M, Linden van der R, Sinden RE, Waters AP, Janse CJ: A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle. Mol Biochem Parasitol. 2004, 137: 23-33. 10.1016/j.molbiopara.2004.04.007.View ArticlePubMedGoogle Scholar
- Dawes EJ, Zhuang S, Sinden RE, Basáñez MG: The temporal dynamics of Plasmodium density through the sporogonic cycle within Anopheles mosquitoes. Trans R Soc Trop Med Hyg. 2009,Google Scholar
- Kaplan EL, Meier P: Non parametric estimation from incomplete observations. J Am Stat Assoc. 1958, 53: 457-481. 10.2307/2281868.View ArticleGoogle Scholar
- Collett D: Modelling Survival Data in Medical Research. 2003, London: Chapman and Hall, 2Google Scholar
- Mantel N, Haenszel W: Statistical aspects of the analysis of data from retrospective studies of disease. J Natl Cancer Inst. 1959, 22: 719-748.PubMedGoogle Scholar
- Armitage P, Berry G: Statistical Methods in Medical Research. 1994, Oxford: Blackwell Scientific Publications, 3Google Scholar
- Basáñez MG, Townson JR, Williams JR, Frontado H, Villamizar NJ, Anderson RM: Density-dependent processes in the transmission of human onchocerciasis: relationship between microfilarial intake and mortality of the simuliid vector. Parasitology. 1996, 113: 331-355. 10.1017/S003118200006649X.View ArticlePubMedGoogle Scholar
- R Development Core Team: R: A language and environment for statistical computing. 2005, R Foundation for Statistical Computing: Vienna, Austria, ISBN 3-900051-07-0, [http://www.R-project.org]Google Scholar
- Bolker BM: Ecological Models in R. 2008, Princeton: Princeton University PressGoogle Scholar
- Macey RI, Oster GF: Berkeley Madonna. 1997, University of California: Berkeley, [http://www.berkeleymadonna.com]Google Scholar
- Harrington LC, Vermeylen F, Jones JJ, Kitthawee S, Sithiprasasna R: Age-dependent survival of the dengue vector Aedes aegypti (Diptera: Culicidae) demonstrated by simultaneous release-recapture of different age cohorts. J Med Entomol. 2008, 45: 307-313. 10.1603/0022-2585(2008)45[307:ASOTDV]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Pumpuni CB, Demaio J, Kent M, Davis JR, Beier JC: Bacterial population dynamics in three anopheline species: the impact on Plasmodium sporogonic development. Am J Trop Med Hyg. 1996, 54: 214-218.PubMedGoogle Scholar
- Luckhart S, Crampton AL, Zamora R, Lieber MJ, Dos Santos PC, Peterson TML, Emmith N, Lim J, Wink DA, Vodovotz Y: Mammalian transforming growth factor beta1 activated after ingestion by Anopheles stephensi modulates mosquito immunity. Infect Immun. 2003, 71: 3000-3009. 10.1128/IAI.71.6.3000-3009.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Gillies MT: Anopheline mosquitoes: vector behaviour and bionomics. Malaria: Principles and Practice of Malariology. Edited by: Wernsdorfer WH, McGregor I. 1988, Edinburgh: Churchill Livingstone, 453-485.Google Scholar
- Hancock PA, Thomas MB, Godfray HC: An age-structured model to evaluate the potential of novel malaria-control interventions: a case study of fungal biopesticide sprays. Proc Biol Sci. 2009, 276: 71-80. 10.1098/rspb.2008.0689.PubMed CentralView ArticlePubMedGoogle Scholar
- Krebs CJ: Ecology. The experimental analysis of distribution and abundance. 1972, New York: Harper & RowGoogle Scholar
- Detinova TS: Age-grouping methods in Diptera of medical importance with specific reference to some vectors of malaria. Monograph Series. World Health Organization. 1962, 47: 1-216.Google Scholar
- Polovodova VP: The determination of the physiological age of female Anopheles, by the number of gonotrophic cycles completed. Med Parazitol (Mosk). 1949, 18: 352-355.Google Scholar
- Hugo LE, Quick-Miles S, Kay BH, Ryan PA: Evaluations of mosquito age grading techniques based on morphological changes. J Med Entomol. 2008, 45: 353-369. 10.1603/0022-2585(2008)45[353:EOMAGT]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Nayar JK, Sauerman DM: A comparative study of flight performance and fuel utilization as a function of age in females of Florida mosquitoes. J Insect Physiol. 1973, 19: 1977-1988. 10.1016/0022-1910(73)90192-3.View ArticlePubMedGoogle Scholar
- Beckett EB: Development and aging of the salivary glands of adult female Aedes aegypti (L.) and Aedes togoi (Theobald) mosquitoes (Diptera: Culicidae). Int J Insect Morphol Embryol. 1990, 19: 277-290. 10.1016/0020-7322(90)90013-F.View ArticleGoogle Scholar
- Christensen BM, LaFond MM, Christensen LA: Defence reactions of mosquitoes to filarial worms: Effect of host age on the immune response to Dirofilaria immitis microfilariae. J Parasitol. 1986, 72: 212-215. 10.2307/3281593.View ArticlePubMedGoogle Scholar
- Hillyer JF, Schmidt SL, Fuchs JF, Boyle JP, Christensen BM: Age-associated mortality in immune challenged mosquitoes (Aedes aegypti) correlated with a decrease in haemocyte numbers. Cell Microbiol. 2005, 7: 39-51. 10.1111/j.1462-5822.2004.00430.x.View ArticlePubMedGoogle Scholar
- Lines JD, Nassor NS: DDT resistance in Anopheles gambiae declines with mosquito age. Med Vet Entomol. 1991, 5: 261-265. 10.1111/j.1365-2915.1991.tb00550.x.View ArticlePubMedGoogle Scholar
- Hazelton GA, Lang CA: Glutathione levels during the mosquito life span with emphasis on senescence. Proc Soc Exp Biol Med. 1984, 176: 249-256.View ArticlePubMedGoogle Scholar
- Maier WA, Becker-Feldman H, Seitz HM: Pathology of malaria-infected mosquitoes. Parasitol Today. 1987, 3: 216-218. 10.1016/0169-4758(87)90063-9.View ArticlePubMedGoogle Scholar
- Han YS, Barillas-Mury C: Implications of Time Bomb model of ookinete invasion of midgut cells. Insect Biochem Mol Biol. 2002, 32: 1311-1316. 10.1016/S0965-1748(02)00093-0.View ArticlePubMedGoogle Scholar
- Han YS, Thompson J, Kafatos FC, Barillas-Mury C: Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei: The Time Bomb theory of ookinete invasion of mosquitoes. EMBO J. 2000, 19: 6030-6040. 10.1093/emboj/19.22.6030.PubMed CentralView ArticlePubMedGoogle Scholar
- Ecker A, Pinto SB, Baker KW, Kafatos FC, Sinden RE: Plasmodium berghei: Plasmodium perforin-like protein 5 is required for mosquito midgut invasion in Anopheles stephensi. Exp Parasitol. 2007, 116: 504-508. 10.1016/j.exppara.2007.01.015.PubMed CentralView ArticlePubMedGoogle Scholar
- Krishnamoorthy K, Subramanian S, Van Oortmarssen GJ, Habbema JDF, Das PK: Vector survival and parasite infection: the effect of Wuchereria bancrofti on its vector Culex quinquefasciatus. Parasitology. 2004, 129: 43-50. 10.1017/S0031182004005153.View ArticlePubMedGoogle Scholar
- Saporu FWO: Analysis of survival data for Simulium damnosum using the regression method. Ann Trop Med Parasitol. 1993, 87: 563-569.PubMedGoogle Scholar
- Das PK, Subramanian S, Manoharan A, Ramaiah KD, Vanamail P, Grenfell BT, Bundy DAP, Michael E: Frequency distribution of Wuchereria bancrofti infection in the vector host in relation to human host: evidence for density dependence. Acta Trop. 1995, 60: 159-165. 10.1016/0001-706X(95)00123-V.View ArticlePubMedGoogle Scholar
- Samarawickrema WA, Laurence BR: Loss of filarial larvae in a natural mosquito population. Ann Trop Med Parasitol. 1978, 72: 561-565.PubMedGoogle Scholar
- Ramasamy MS, Kulasekera R, Wanniarachchi IC, Srikrishnaraj KA, Ramasamy R: Interactions of human malaria parasites, Plasmodium vivax and P. falciparum, with the midgut of Anopheles mosquitoes. Med Vet Entomol. 1997, 11: 290-296. 10.1111/j.1365-2915.1997.tb00409.x.View ArticlePubMedGoogle Scholar
- Vaughan JA, Turell MJ: Facilitation of Rift Valley fever virus transmission by Plasmodium berghei sporozoites in Anopheles stephensi mosquitoes. Am J Trop Med Hyg. 1996, 55: 407-409.PubMedGoogle Scholar
- Beier JC: Malaria parasite development in mosquitoes. Annu Rev Entomol. 1998, 43: 519-543. 10.1146/annurev.ento.43.1.519.View ArticlePubMedGoogle Scholar
- Hurd H, Hogg JC, Renshaw M: Interactions between bloodfeeding, fecundity and infection in mosquitoes. Parasitol Today. 1995, 11: 411-416. 10.1016/0169-4758(95)80021-2.View ArticleGoogle Scholar
- Rivero A, Ferguson HM: The energetic budget of Anopheles stephensi infected with Plasmodium chabaudi: is energy depletion a mechanism for virulence?. Proc R Soc Lond B. 2003, 270: 1365-1371. 10.1098/rspb.2003.2389.View ArticleGoogle Scholar
- Dimopoulos G, Christophides GK, Meister S, Schultz J, White KP, Barillas-Mury C, Kafatos FC: Genome expression analysis of Anopheles gambiae: responses to injury, bacterial challenge, and malaria infection. Proc Natl Acad Sci USA. 2002, 99: 8814-8819. 10.1073/pnas.092274999.PubMed CentralView ArticlePubMedGoogle Scholar
- Michel K, Kafatos FC: Mosquito immunity against Plasmodium. Insect Biochem Mol Biol. 2005, 35: 677-689. 10.1016/j.ibmb.2005.02.009.View ArticlePubMedGoogle Scholar
- Richman AM, Dimopoulos G, Seeley D, Kafatos FC: Plasmodium activates the innate immune response of Anopheles gambiae mosquitoes. EMBO J. 1997, 16: 6114-6119. 10.1093/emboj/16.20.6114.PubMed CentralView ArticlePubMedGoogle Scholar
- Barillas-Mury C, Wizel B, Han YS: Mosquito immune responses and malaria transmission: lessons from insect model systems and implications for vertebrate innate immunity and vaccine development. Insect Biochem Mol Biol. 2000, 30: 429-442. 10.1016/S0965-1748(00)00018-7.View ArticlePubMedGoogle Scholar
- Schwartz A, Koella JC: The cost of immunity in the yellow fever mosquito, Aedes aegypti depends on immune activation. J Evol Biol. 2004, 17: 834-840. 10.1111/j.1420-9101.2004.00720.x.View ArticlePubMedGoogle Scholar
- Ahmed AM, Hurd H: Immune stimulation and malaria infection impose reproductive costs in Anopheles gambiae viz follicular apoptosis. Microb Infect. 2006, 8: 308-315. 10.1016/j.micinf.2005.06.026.View ArticleGoogle Scholar
- Dimopoulos G, Richman A, Müller H-M, Kafatos FC: Molecular immune responses of the mosquito Anopheles gambiae to bacteria and malaria parasites. Proc Natl Acad Sci USA. 1997, 94: 11508-11513. 10.1073/pnas.94.21.11508.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson RA, Koella JC, Hurd H: The effect of Plasmodium yoelii nigeriensis infection on the feeding persistence of Anopheles stephensi Liston throughout the sporogonic cycle. Proc R Soc Lond B. 1999, 266: 729-733. 10.1098/rspb.1999.0818.View ArticleGoogle Scholar
- Koella JC, Sorensen FL, Anderson RA: The malaria parasite, Plasmodium falciparum, increases the frequency of multiple feeding of its mosquito vector, Anopheles gambiae. Proc R Soc Lond B. 1998, 265: 763-768. 10.1098/rspb.1998.0358.View ArticleGoogle Scholar
- Rossignol PA, Ribeiro JMC, Spielman A: Increased intradermal probing time in sporozoite-infected mosquitoes. Am J Trop Med Hyg. 1984, 33: 17-20.PubMedGoogle Scholar
- Rossignol PA, Ribeiro JM, Spielman A: Increased biting rate and reduced fertility in sporozoite-infected mosquitoes. Am J Trop Med Hyg. 1986, 35: 277-279.PubMedGoogle Scholar
- Scheifer BA, Ward RA, Eldridge BF: Plasmodium cynomolgi: Effects of malaria infection on laboratory flight performance of Anopheles stephensi mosquitoes. Exp Parasitol. 1977, 41: 397-404. 10.1016/0014-4894(77)90111-4.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.