Non-falciparum malaria infections in pregnant women in West Africa
- John Williams1,
- Fanta Njie2,
- Matthew Cairns3,
- Kalifa Bojang2,
- Sheick Oumar Coulibaly4,
- Kassoum Kayentao5,
- Ismaela Abubakar3,
- Francis Akor2,
- Khalifa Mohammed2,
- Richard Bationo4,
- Edgar Dabira4,
- Alamissa Soulama4,
- Moussa Djimdé5,
- Etienne Guirou5,
- Timothy Awine1,
- Stephen L. Quaye1,
- Jaume Ordi6,
- Ogobara Doumbo5,
- Abraham Hodgson1,
- Abraham Oduro1,
- Pascal Magnussen7,
- Feiko O. ter Kuile8,
- Arouna Woukeu3,
- Paul Milligan3,
- Harry Tagbor9,
- Brian Greenwood3 and
- Daniel Chandramohan3Email author
© Williams et al. 2016
Received: 9 October 2015
Accepted: 10 January 2016
Published: 29 January 2016
Non-Plasmodium falciparum malaria infections are found in many parts of sub-Saharan Africa but little is known about their importance in pregnancy.
Blood samples were collected at first antenatal clinic attendance from 2526 women enrolled in a trial of intermittent screening and treatment of malaria in pregnancy (ISTp) versus intermittent preventive treatment (IPTp) conducted in Burkina Faso, The Gambia, Ghana and Mali. DNA was extracted from blood spots and tested for P. falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale using a nested PCR test. Risk factors for a non-falciparum malaria infection were investigated and the influence of these infections on the outcome of pregnancy was determined.
P. falciparum infection was detected frequently (overall prevalence by PCR: 38.8 %, [95 % CI 37.0, 40.8]), with a prevalence ranging from 10.8 % in The Gambia to 56.1 % in Ghana. Non-falciparum malaria infections were found only rarely (overall prevalence 1.39 % [95 % CI 1.00, 1.92]), ranging from 0.17 % in the Gambia to 3.81 % in Mali. Ten non-falciparum mono-infections and 25 mixed falciparum and non-falciparum infections were found. P. malariae was the most frequent non-falciparum infection identified; P. vivax was detected only in Mali. Only four of the non-falciparum mono-infections were detected by microscopy or rapid diagnostic test. Recruitment during the late rainy season and low socio-economic status were associated with an increased risk of non-falciparum malaria as well as falciparum malaria. The outcome of pregnancy did not differ between women with a non-falciparum malaria infection and those who were not infected with malaria at first ANC attendance.
Non-falciparum infections were infrequent in the populations studied, rarely detected when present as a mono-infection and unlikely to have had an important impact on the outcome of pregnancy in the communities studied due to the small number of women infected with non-falciparum parasites.
Plasmodium falciparum is the dominant malaria parasite in most parts of sub-Saharan Africa and hence responsible for most infections in pregnancy . Plasmodium vivax is also a significant cause of maternal morbidity during pregnancy and of low birth weight in parts of Asia [2–5] and Latin America [6, 7], even though the parasite does not sequester in the placenta . P. vivax has been reported from time to time in non-pregnant populations in sub-Saharan Africa, especially when polymerase chain reaction (PCR) assays have been used [9–11], but P. vivax infections are generally considered to be uncommon in sub-Saharan Africa because of the presence in a high proportion of the population of Duffy antigen negative red cells which cannot be invaded by this malaria parasite. P. vivax infections have been recorded only rarely during pregnancy in East or West Africa , although they are relatively prevalent in parts of Ethiopia . In contrast, Plasmodium ovale and Plasmodium malariae are relatively common in many parts of East and West Africa causing a significant proportion of febrile malaria episodes  and asymptomatic infections . However, very little is known about the potential impact of non-falciparum malaria infections on pregnancy and there have been very few reports of non-falciparum infections in pregnant women from East or West Africa. Walker-Abbey et al.  detected P. malariae and P. ovale in 6.9 and 1.8 % of pregnant Cameroonian women respectively, nearly always in mixed infections with P. falciparum. Mockenhaupt et al.  reported an increased susceptibility of pregnant Ghanaian women with alpha + thalassaemia to infection with P. malariae and Coldren et al.  describe a relapsing case of P. ovale in a pregnant women which was probably acquired many years previously in East or West Africa.
Screening of pregnant women at ante-natal clinic (ANC) visits with a rapid diagnostic test (RDT) for malaria and treatment of those who are positive with artemisinin-based combination therapy, an approach termed intermittent screening and treatment (ISTp), is being considered as a potential approach to the control of malaria in some epidemiological situations such as low transmission areas where few women are likely to benefit from currently recommended intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP). However, there is concern that this approach may lead to some malaria infections, especially those present at low density, being missed and adversely affecting the outcome of pregnancy. This is especially likely to be the case for non-falciparum infections which are often present at only a low density and for which many RDTs have poor sensitivity. For this reason, the prevalence of falciparum and non-falciparum infections at first ANC attendance was investigated in women enrolled in a trial of ISTp versus IPTp-SP conducted in four West African countries (Burkina Faso, The Gambia, Ghana and Mali) and determined how many of these infections were detected by an RDT.
Details of the way in which the trial of ISTp versus IPTp-SP was conducted have been presented previously . In brief, 5354 primigravid or secundigravid women were randomised individually at their first ANC visit to the ISTp or IPTp-SP group. Women in the ISTp group were screened for malaria with a RDT at enrollment and at each subsequent routine ANC visit and treated with artemether–lumefantrine (AL) if positive, whilst women in the IPTp group received a dose of SP at each routine ANC visit. Haemoglobin (Hb) concentration was determined at each woman’s final ANC visit, scheduled to occur between 36 and 40 weeks of gestation. Study women were encouraged to deliver in hospital where placental biopsies were obtained for histology and examined using a standardized protocol as described previously . Birth weight was recorded at delivery in women who delivered in hospital and within 7 days of delivery in women who delivered at home. Infants and mothers were evaluated 6 weeks after birth.
The analysis reported in this paper is based on blood samples obtained at the first ANC visit from the 2526 women in the ISTp-AL group. An RDT (First Response® Malaria Rapid Diagnostic Combo Test) was done and blood films and filter paper blots prepared. Blood films were examined for malaria parasites according to the methods described by Swysen et al. . PCR testing was conducted at the MRC Unit, The Gambia. Following extraction of DNA from a filter paper blood spot, the first stage of this nested PCR test involves a PCR assay with the primers rPLU5 + rPLU6. This assay includes positive controls for each of the four main malaria species (P. falciparum, P. vivax, P. ovale and P. malariae) and a negative control without DNA. The second PCR reaction detects the presence of Plasmodium infection using the genus specific primers Plasmo-1 and Plasmo-2. The third stage, undertaken on genus specific samples, determines species by running four parallel PCR assays using the following primers (P. falciparum: rFAL 1 + rFAL 2; P. malariae: rMAL 1 + rMAL 2; P. ovale: rOVA 1 + rOVA 2; P. vivax:rVIV 1 + rVIV 2). For further differentiation of P. ovale into the two distinct species P. ovale curtisi and P. ovale wallikeri, the P. ovale sp. Tryptophan-rich antigen (PoTRA) nested PCR assay was used . Quality control of the assays undertaken in The Gambia was undertaken at Duke University, North Carolina. The lower level of detection of the assay is approximately 10 parasites per µl. The sensitivity of microscopy and an RDT in detecting non-falciparum infections detected by PCR was explored.
The association between demographic and obstetric factors and the presence of falciparum and non-falciparum malaria infections was explored. A multi-variable logistic regression model was then built to explore risk factors for non-falciparum infection, controlling for potential confounding from other risk factors, using those without a malaria infection as the reference group. Women with falciparum malaria infection were excluded from the analysis, as many of the risk factors for P. falciparum and non-falciparum infections are likely to be shared. An exploratory analysis was conducted to investigate whether there was any evidence of an association between non-falciparum malaria infections and the primary outcomes of the main trial: active malaria infection of the placenta, low birth weight and haemoglobin at the last ANC contact prior to delivery, again using those with no malaria infection as the reference group. The relationship with measured birth weight, an important secondary end-point for the trial, was also explored.
The trial which provided the samples for this study was approved by the London School of Hygiene and Tropical Medicine Ethical Committee and by the ethics committees of all the partner African institutions: Comité National d’Ethique pour la Recherche en Santé (CNERS), Burkina Faso; Ethics committee of the Ghana Health Service Committee Research & Development Division, Ghana Health Science, Ghana; Navrongo Health Research Centre Institutional Review Board, Ghana; Comité d’éthique de la Faculté de la Faculté de Médecine et d’Odontostomatologie, Mali; The Gambia Government/MRC Laboratories Joint Ethics Committee, The Gambia. Written informed consent was obtained from all participating women. The trial is registered with ClinicalTrials.gov (NCT01084213) and the Pan African Clinical trials Registry (PACT201202000272122).
Falciparum and non-falciparum malaria infections detected in 2526 pregnant West African women at first ANC clinic attendance
Number of women
Number of P. falciparum infections
Prevalence of P. falciparum
(95 % Confidence interval)
Number of non-falciparum malaria infections overall
Prevalence of non-falciparum malaria
(95 % Confidence interval)
Number of mono-infections
P. ovale curtisi
P. ovale wallikeri
P. falciparum, P. malariae
P. falciparum, P. ovale curtisi
P. falciparum, P. ovale wallikeri
P. falciparum, P. vivax
P. falciparum, P. malariae, P. ovale curtisi
P. falciparum, P. malariae, P. ovale wallikeri
Plasmodium malariae was the most common non-falciparum species detected, being present alone in three women (all from Mali) and in 14 mixed infections (11 with P. falciparum, and three with P. falciparum and P. ovale). Eleven mixed P. ovale and P. falciparum infections were detected. Both forms of P. ovale were found in roughly equal abundance (seven P. ovale curtisi, nine P. ovale wallikeri, and one either mixed infection of P. ovale curtisi with P. ovale wallikeri, or an indeterminate P. ovale infection). P. vivax was found in only four women (two mixed with P. falciparum), all of whom lived in Mali. Because of the unexpected finding of P. vivax parasites in four black, African women resident near Bamako, the identity of these parasites was confirmed using a real-time PCR assay targeting the P. vivax ribosomal RNA gene (S.R. Meshnick, personal communication).
There was variation in the prevalence of non-falciparum malaria between study sites, with Mali having the highest prevalence (3.81 % [95 % CI 2.55, 5.68]) compared with Ghana (0.96 % [95 % CI 0.43, 2.13]), Burkina Faso (0.72 % [95 % CI 0.30, 1.72]) and The Gambia (0.17 % [95 % CI 0.02, 1.17]).
The prevalence of P. falciparum and non-falciparum malaria infections overall at first ANC visit according to baseline characteristics
P. falciparum infection
Under 18 years
25 years +
Early wet (6–8)
Late wet (9–11)
Early dry (12–2)
Late dry (3–5)
Risk factors for non-falciparum malaria infections overall at first ANC attendance
Crude OR (95 % CI)
Adjusted OR (95 % CI)
0.74 (0.22, 2.46)
0.53 (0.14, 2.03)
0.08 (0.01, 0.69)
0.10 (0.01, 0.88)
2.52 (1.01, 6.28)
6.54 (1.79, 23.9)
0.85 (0.42, 1.69)
0.71 (0.33, 1.54)
1.51 (0.70, 3.23)
1.92 (0.85, 4.31)
0.57 (0.12, 2.64)
0.60 (0.12, 2.90)
Under 18 years
2.98 (0.86, 10.3)
3.77 (1.03, 13.8)
1.38 (0.32, 5.83)
2.14 (0.43, 10.6)
25 years +
1.75 (0.36, 8.41)
2.54 (0.45, 14.3)
0.92 (0.43, 1.96)
0.96 (0.41, 2.22)
0.27 (0.06, 1.23)
0.27 (0.05, 1.32)
1.36 (0.17, 11.2)
3.73 (0.35, 40.2)
Early wet (6–8)
5.59 (2.10, 14.9)
7.10 (2.56, 19.7)
Early dry (12–2)
2.84 (0.97, 8.27)
2.54 (0.84, 7.67)
1.76 (0.66, 4.69)
1.85 (0.68, 5.04)
2.14 (0.85, 5.34)
1.82 (0.68, 4.83)
1.07 (0.21, 5.33)
0.96 (0.18, 5.02)
6.30 (1.83, 21.7)
6.48 (1.68, 25.0)
5.89 (1.35, 25.7)
6.55 (1.27, 33.7)
Association of non-falciparum malaria overall with birth outcomes including placental malaria (PM), birth-weight and haemoglobin concentration
No malaria infection
No active PM
Crude OR (95 % CI), p value
1.06 (0.41, 2.76)
Adjusted OR (95 % CI), p value
1.63 (0.58, 4.60)
Normal birth weight
Low birth weight
Crude OR (95 % CI), p value
0.97 (0.36, 2.57)
Adjusted OR (95 % CI), p value
0.95 (0.35, 2.58)
Birth weight (kg)
Crude difference, grammes (95 % CI), p value
−57.7 (−227.7, 112.3)
Adjusted difference, grammes (95 % CI), p value
−51.9 (−220.8, 117.0)
Hb at final ANC
Crude difference, g/dL (95 % CI), p value
−0.09 (−0.64, 0.47)
Adjusted difference, g/dL (95 % CI), p value
0.01 (−0.55, 0.56)
This study confirmed that non-falciparum malaria infections are present in pregnant women in West Africa but that they are uncommon, with an overall prevalence across four countries of 1.4 % at first ANC attendance. It is likely that some additional infections occurred later in pregnancy. However, among the women from Ghana enrolled in this study who were followed throughout pregnancy, at least a half of all malaria infections were detected at the first ANC visit ; the figures for all ANC visits were 53.6 % for RDT, 60.6 % for microscopy and 64.3 % for PCR respectively. Thus, unless there is a differential rate of infection between non-falciparum and falciparum infections, it is likely that the prevalence of non-falciparum infections at any time point during the course of pregnancy would have been be low in the study population. Prevalence of non-falciparum infections varied between centres, being less than 1 % in three, but close to 4 % in Mali. These differences cannot be explained solely by differences in the intensity of transmission between centres as Mali had the second lowest prevalence of P. falciparum infection of the four study sites. P. vivax was detected only in Mali, and only in one sub-site in suburban Bamako. This malaria parasite is known to be present in northern Mali , perhaps because of some mixing with north African populations, but not in the southern parts of the country where all these infections were found. This unexpected finding is being followed up further.
The results of this study indicate the difficulty in identifying non-falciparum malaria infections by microscopy when these infections are encountered rarely and often present in mixed infections with P. falciparum: only three of the 10 pure non-falciparum infections were detected by microscopy, and two of these were mistaken for P. falciparum despite the fact that study slides were read by two well-trained microscopists and that the quality of microscopical reading was well controlled. In sub-Saharan Africa, microscopists expect malaria infections present in a blood film to be due to P. falciparum and this may have influenced their recording of the results. Rapid diagnostic tests did not perform any better than microscopy, also missing seven of the 10 non-falciparum mono-infections, perhaps because these were present at only a low density. When P. falciparum was also present, RDTs detected all but two of these infections, whereas microscopy missed seven of the mixed infections. These findings suggest that when, in sub-Saharan Africa, detection of non-falciparum malaria infection in pregnancy depends upon microscopy, many non-falciparum infections will be missed. Factors likely to have contributed to the poor performance of microscopy and a RDT in detecting non-falciparum infections are their frequent occurrence as mixed infections with P. falciparum and their presence at only a low density. Why the latter should be the case is uncertain, but it may be due in part to suppression of the minor parasite by P. falciparum in mixed infections and in part due to the fact these may have been more chronic infections than those caused by P. falciparum.
Enrolment at the end of the wet season was a risk factor for non-falciparum malaria infection, as was lower socio-economic status. These are both well-established risk factors for malaria infection in general. The suggestion of an association of one specific age group with higher risk of non-falciparum infections does not have a biological explanation and is likely to be a chance finding as the numbers of non-falciparum infections studied was small.
There was no association between the presence of a non-falciparum malaria infection at first ANC presentation and an adverse outcome of pregnancy. However, the number of non-falciparum infections was small, 26 of the 35 individuals with non-falciparum infections at baseline were treated with artemether-lumefantrine and it is possible that some women in the uninfected reference group were infected at later time points, diluting differences between the two groups, so this finding is not surprising.
This study was undertaken as part of an evaluation of ISTp because of concerns that a significant number of infections caused by non-falciparum species might be missed during RDT testing, with an adverse impact on the outcome of pregnancy, if this approach to malaria control in pregnancy was to be implemented. Because the majority of non-falciparum infections occurred in conjunction with a falciparum infection which was detected by a RDT, as noted in another recent study in Burkina Faso and Uganda (Hopkins et al. personal communication), the majority of women with a non-falciparum infection received appropriate treatment; only seven non-falciparum mono-infections and two mixed non-falciparum and falciparum infections were missed at first ANC visit using a ‘combo’ RDT among 2526 women. For this reason the occurrence of non-falciparum infections is unlikely to be a concern should ISTp be recommended for any population group in sub-Saharan Africa. However, this might not be the case in other parts of the malaria endemic world where non-falciparum infections, particularly those caused by P. vivax, are more prevalent and where many more infections might be missed using currently available RDTs.
JW, KB, SOO, KK, IA, FA, KM, RB, ED, AS, MD, EG, TA, SQ, OD, AH, AO conducted the clinical trial during which samples on which this study was based were collected. FN and JW conducted the molecular analysis and JO supervised placental histology. AW was responsible for data management, MC and P Milligan for statistical analysis. P Magnussen, F ter K, HT, DC and BG contributed to the conception and design of the study. All authors read and approved the final manuscript.
We thank the women who participated in the trial, the many field, laboratory and data management staff at each of the trial sites who contributed to its successful outcome and the Ministries of Health which supported this study. We thank Manuela Claite for her administrative support for the trial. We also thank members of the Data Safety and Monitoring Board (Prof. GAT Targett [chair]; Dr. GA Quansah-Asare, Prof. F Little, Prof. O Nyan, Dr. C Ouedraogo and Prof. S Sow). Raouf Osseni and Adama Gansane were the trial’s clinical monitors. High quality placental histology slides were prepared by the staff of the Department of Pathology, Korle Bu Hospital, Accra, Ghana. We thank Professors Steve Meshnik, Steve Taylor and Colin Sutherland for assistance with the PCR assays and Dr María Rupérez for helpful comments on the paper. The study was funded by the European Developing Country Clinical Trials Programme (Grant Number IP.2007.31080.003) and by the Malaria in Pregnancy Consortium which is funded through a grant from the Bill and Melinda Gates Foundation to the Liverpool School of Tropical Medicine. MC is supported by a fellowship jointly funded the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement.
The authors declare that they have no competing interests.
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