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  1. Research

    Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

    The presence of Plasmodium falciparum gametocytes in peripheral blood is essential for human to mosquito parasite transmission. The detection of submicroscopic infections with gametocytes and the estimation of th...

    Federica Santolamazza, Pamela Avellino, Giulia Siciliano, Franck Adama Yao, Fabrizio Lombardo, Jean Bosco Ouédraogo, David Modiano, Pietro Alano and Valentina Dianora Mangano

    Malaria Journal 2017 16:468

    Published on: 17 November 2017

  2. Research

    Monitoring of long-lasting insecticidal nets (LLINs) coverage versus utilization: a community-based survey in malaria endemic villages of Central India

    Despite the known effectiveness of long-lasting insecticidal nets (LLINs) in providing protection against malaria, high level of ownership and use are very difficult to achieve and maintain. Nearly 40,000 LLIN...

    Kamaraju Raghavendra, Mehul Kumar Chourasia, Dipak Kumar Swain, Rajendra M. Bhatt, Sreehari Uragayala, G. D. P. Dutta and Immo Kleinschmidt

    Malaria Journal 2017 16:467

    Published on: 17 November 2017

  3. Research

    Singapore’s Anopheles sinensis Form A is susceptible to Plasmodium vivax isolates from the western Thailand–Myanmar border

    Singapore has been certified malaria-free by the World Health Organization since November 1982. However, sporadic autochthonous malaria outbreaks do occur. In one of the most recent outbreaks of vivax malaria,...

    Sook-Cheng Pang, Chiara Andolina, Benoit Malleret, Peter R. Christensen, Sai-Gek Lam-Phua, Muhammad Aliff Bin Abdul Razak, Chee-Seng Chong, Daiqin Li, Cindy S. Chu, Bruce Russell, Laurent Rénia, Lee-Ching Ng and Francois Nosten

    Malaria Journal 2017 16:465

    Published on: 16 November 2017

  4. Research

    Age-specific Plasmodium parasite profile in pre and post ITN intervention period at a highland site in western Kenya

    Monitoring and evaluation of entomological, parasitological and clinical data is an important component of malaria control as it is a measure of the success of the interventions. In many studies, clinical data...

    Ednah N. Ototo, Guofa Zhou, Lucy Kamau, Jenard P. Mbugi, Christine L. Wanjala, Maxwell Machani, Harrysone Atieli, Andrew K. Githeko and Guiyun Yan

    Malaria Journal 2017 16:466

    Published on: 16 November 2017

  5. Research

    Synthetic oleanane triterpenoids enhance blood brain barrier integrity and improve survival in experimental cerebral malaria

    Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection associated with high mortality and neurocognitive impairment in survivors. New anti-malarials and host-based adjunctive therapy ma...

    Valerie M. Crowley, Kodjo Ayi, Ziyue Lu, Karen T. Liby, Michael Sporn and Kevin C. Kain

    Malaria Journal 2017 16:463

    Published on: 14 November 2017

  6. Correction

    Correction to: Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique

    After publication of the article [1], it has been brought to our attention that two of the authors have had their names spelt incorrectly in the original publication. The eighth author should be “N. Regina Rabino...

    Himanshu Gupta, Gloria Matambisso, Beatriz Galatas, Pau Cisteró, Lidia Nhamussua, Wilson Simone, Jane Cunningham, N. Regina Rabinovich, Pedro Alonso, Francisco Saute, Pedro Aide and Alfredo Mayor

    Malaria Journal 2017 16:464

    Published on: 14 November 2017

    The original article was published in Malaria Journal 2017 16:416

  7. Research

    Expanding malaria diagnosis and treatment in Lao PDR: lessons learned from a public–private mix initiative

    As in other countries of the Greater Mekong Sub-region (GMS), the private health sector constitutes a significant avenue where malaria services are provided and presents a unique opportunity for public–private...

    Nouannipha Simmalavong, Sengkham Phommixay, Phoudaliphone Kongmanivong, Odai Sichanthongthip, Bouasy Hongvangthong, Deyer Gopinath and David M. Sintasath

    Malaria Journal 2017 16:460

    Published on: 13 November 2017

  8. Research

    Understanding malaria treatment-seeking preferences within the public sector amongst mobile/migrant workers in a malaria elimination scenario: a mixed-methods study

    Migration flows and the emerging resistance to artemisinin-based combination therapy in the Greater Mekong Sub-region (GMS) create programmatic challenges to meeting the AD 2030 malaria elimination target in M...

    Aung Ye Naung Win, Thae Maung Maung, Khin Thet Wai, Tin Oo, Aung Thi, Rungrawee Tipmontree, Ngamphol Soonthornworasiri, Mondha Kengganpanich and Jaranit Kaewkungwal

    Malaria Journal 2017 16:462

    Published on: 13 November 2017

  9. Review

    Mapping multiple components of malaria risk for improved targeting of elimination interventions

    There is a long history of considering the constituent components of malaria risk and the malaria transmission cycle via the use of mathematical models, yet strategic planning in endemic countries tends not to...

    Justin M. Cohen, Arnaud Le Menach, Emilie Pothin, Thomas P. Eisele, Peter W. Gething, Philip A. Eckhoff, Bruno Moonen, Allan Schapira and David L. Smith

    Malaria Journal 2017 16:459

    Published on: 13 November 2017

  10. Research

    Yeast interfering RNA larvicides targeting neural genes induce high rates of Anopheles larval mortality

    Although larviciding can reduce the number of outdoor biting malaria vector mosquitoes, which may help to prevent residual malaria transmission, the current larvicide repertoire is faced with great challenges ...

    Keshava Mysore, Limb K. Hapairai, Longhua Sun, Elizabeth I. Harper, Yingying Chen, Kathleen K. Eggleson, Jacob S. Realey, Nicholas D. Scheel, David W. Severson, Na Wei and Molly Duman-Scheel

    Malaria Journal 2017 16:461

    Published on: 13 November 2017

  11. Research

    Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers

    Both in endemic countries and in imported malaria, changes in total and differential leukocyte count during Plasmodium falciparum infection have been described. To study the exact dynamics of differential leukocy...

    Marlies E. van Wolfswinkel, Marijke C. C. Langenberg, Linda J. Wammes, Robert W. Sauerwein, Rob Koelewijn, Cornelus C. Hermsen, Jaap J. van Hellemond and Perry J. van Genderen

    Malaria Journal 2017 16:457

    Published on: 10 November 2017

  12. Research

    A single rapamycin dose protects against late-stage experimental cerebral malaria via modulation of host immunity, endothelial activation and parasite sequestration

    Maladaptive immune responses during cerebral malaria (CM) result in high mortality despite opportune anti-malarial chemotherapy. Rapamycin, an FDA-approved immunomodulator, protects against experimental cerebr...

    Pedro Mejia, J. Humberto Treviño-Villarreal, Justin S. Reynolds, Mariana De Niz, Andrew Thompson, Matthias Marti and James R. Mitchell

    Malaria Journal 2017 16:455

    Published on: 9 November 2017

  13. Research

    Variation in the immune responses against Plasmodium falciparum merozoite surface protein-1 and apical membrane antigen-1 in children residing in the different epidemiological strata of malaria in Cameroon

    Studies to assess the immune responses against malaria in Cameroonian children are limited. The purpose of this study was to assess the immune responses against Plasmodium falciparum merozoite surface protein-1 (...

    Tebit Emmanuel Kwenti, Adzemye Linus Moye, Adzemye Basil Wiylanyuy, Longdoh Anna Njunda and Theresa Nkuo-Akenji

    Malaria Journal 2017 16:453

    Published on: 9 November 2017

  14. Research

    Individual and household characteristics of persons with Plasmodium falciparum malaria in sites with varying endemicities in Kinshasa Province, Democratic Republic of the Congo

    The Democratic Republic of the Congo (DRC) bears a large share of global malaria burden despite efforts to control and eliminate the disease. More detailed understanding of individual and household level chara...

    Melchior Kashamuka Mwandagalirwa, Lauren Levitz, Kyaw L. Thwai, Jonathan B. Parr, Varun Goel, Mark Janko, Antoinette Tshefu, Michael Emch, Steven R. Meshnick and Margaret Carrel

    Malaria Journal 2017 16:456

    Published on: 9 November 2017

  15. Research

    Temporal variation in confirmed diagnosis of fever-related malarial cases among children under-5 years by community health workers and in health facilities between years 2013 and 2015 in Siaya County, Kenya

    Malaria case management continues to experience dynamic changes. Building community capacity is instrumental in both prevention and treatment of malaria. The World Health Organization (WHO) recommends utilizat...

    Donald O. Apat, John M. Gachohi, Mohamed Karama, Jusper R. Kiplimo and Sonia E. Sachs

    Malaria Journal 2017 16:454

    Published on: 9 November 2017

  16. Research

    Reassessment of asymptomatic carriers of Plasmodium spp. in an endemic area with a very low incidence of malaria in extra-Amazonian Brazil

    Regions with residual transmission are potential obstacles to the elimination of malaria. It is, therefore, essential to understand the factors associated with the maintenance of endemic malaria in these areas...

    Filomena E. C. de Alencar, Rosely dos Santos Malafronte, Crispim Cerutti Jr., Lícia Natal Fernandes, Julyana Cerqueira Buery, Blima Fux, Helder Ricas Rezende and Angelica Espinosa Miranda

    Malaria Journal 2017 16:452

    Published on: 9 November 2017

  17. Research

    Screening a protein kinase inhibitor library against Plasmodium falciparum

    Protein kinases have been shown to be key drug targets, especially in the area of oncology. It is of interest to explore the possibilities of protein kinases as a potential target class in Plasmodium spp., the ca...

    Irene Hallyburton, Raffaella Grimaldi, Andrew Woodland, Beatriz Baragaña, Torsten Luksch, Daniel Spinks, Daniel James, Didier Leroy, David Waterson, Alan H. Fairlamb, Paul G. Wyatt, Ian H. Gilbert and Julie A. Frearson

    Malaria Journal 2017 16:446

    Published on: 7 November 2017

  18. Research

    Plasmodium falciparum variant erythrocyte surface antigens: a pilot study of antibody acquisition in recurrent natural infections

    During intra-erythrocytic replication Plasmodium falciparum escapes the human host immune system by switching expression of variant surface antigens (VSA). Piecemeal acquisition of variant specific antibody respo...

    Elise Schieck, E. Jane Poole, Anja Rippert, Judy Peshu, Philip Sasi, Steffen Borrmann and Peter C. Bull

    Malaria Journal 2017 16:450

    Published on: 7 November 2017

  19. Research

    Malaria vectors in the Democratic Republic of the Congo: the mechanisms that confer insecticide resistance in Anopheles gambiae and Anopheles funestus

    The Democratic Republic of the Congo (DRC) is characterized as a holoendemic malaria area with the main vectors being Anopheles funestus and members of the Anopheles gambiae complex. Due to political instability ...

    Luisa Nardini, Richard H. Hunt, Yael L. Dahan-Moss, Nanette Christie, Riann N. Christian, Maureen Coetzee and Lizette L. Koekemoer

    Malaria Journal 2017 16:448

    Published on: 7 November 2017

  20. Research

    Evaluation of the Deki Reader™, an automated RDT reader and data management device, in a household survey setting in low malaria endemic southwestern Uganda

    Early diagnosis of suspected malaria cases with a rapid diagnostic test (RDT) has been shown to be an effective malaria control tool used in many resource-constrained settings. However, poor quality control an...

    Caesar Oyet, Michelle E. Roh, Gertrude N. Kiwanuka, Patrick Orikiriza, Martina Wade, Sunil Parikh, Juliet Mwanga-Amumpaire and Yap Boum II

    Malaria Journal 2017 16:449

    Published on: 7 November 2017

  21. Methodology

    Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2

    Rapid diagnostic test (RDT) positivity is supplanting microscopy as the standard measure of malaria burden at the population level. However, there is currently no standard for externally validating RDT results...

    Mateusz Plucinski, Rafael Dimbu, Baltazar Candrinho, James Colborn, Aida Badiane, Daouda Ndiaye, Kimberly Mace, Michelle Chang, Jean F. Lemoine, Eric S. Halsey, John W. Barnwell, Venkatachalam Udhayakumar, Michael Aidoo and Eric Rogier

    Malaria Journal 2017 16:451

    Published on: 7 November 2017

  22. Research

    Indoor and outdoor malaria vector surveillance in western Kenya: implications for better understanding of residual transmission

    The widespread use of indoor-based malaria vector control interventions has been shown to alter the behaviour of vectors in Africa. There is an increasing concern that such changes could sustain residual trans...

    Teshome Degefa, Delenasaw Yewhalaw, Guofa Zhou, Ming-chieh Lee, Harrysone Atieli, Andrew K. Githeko and Guiyun Yan

    Malaria Journal 2017 16:443

    Published on: 6 November 2017

  23. Correction

    Correction to: Absence of Plasmodium inui and Plasmodium cynomolgi, but detection of Plasmodium knowlesi and Plasmodium vivax infections in asymptomatic humans in the Betong division of Sarawak, Malaysian Borneo

    After publication of the article [1], it has been brought to our attention that two of the labels on Figure 4 have transposed. The labels “S-type SSU rRNA” and “A-type SSU rRNA” should be in opposite places.

    Angela Siner, Sze-Tze Liew, Khamisah Abdul Kadir, Dayang Shuaisah Awang Mohamad, Felicia Kavita Thomas, Mohammad Zulkarnaen and Balbir Singh

    Malaria Journal 2017 16:445

    Published on: 6 November 2017

    The original article was published in Malaria Journal 2017 16:417

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