Open Access

In vitro activity of ferroquine (SSR 97193) against Plasmodium falciparum isolates from the Thai-Burmese border

  • Marion Barends1, 2,
  • Anchalee Jaidee1,
  • Nopparat Khaohirun1,
  • Pratap Singhasivanon2 and
  • François Nosten1, 2, 3Email author
Malaria Journal20076:81

https://doi.org/10.1186/1475-2875-6-81

Received: 01 March 2007

Accepted: 27 June 2007

Published: 27 June 2007

Abstract

Background

On the borders of Thailand, Plasmodium falciparum has become resistant to nearly all available drugs, and there is an urgent need to find new antimalarial drugs or drug combinations. Ferroquine (SSR97193) is a new 4-aminoquinoline antimalarial active against chloroquine resistant and sensitive P. falciparum strains in vivo and in vitro. This antimalarial organic iron complex (a ferrocenyl group has been associated with chloroquine) is meant to use the affinity of Plasmodium for iron to increase the probability for encountering the anti-malarial molecule.

The aim of the present study was to investigate the activity of ferroquine against P. falciparum isolates from an area with a known high multi-drug resistance rate.

Methods

Parasite isolates were obtained from patients with acute falciparum malaria attending the clinics of SMRU. In vitro cultures of these isolates were set-up in the SMRU-laboratory on pre-dosed drug plates, and grown in culture for 42 hours. Parasite growth was assessed by the double-site enzyme-linked pLDH immunodetection (DELI) assay.

Results

Sixty-five P. falciparum isolates were successfully grown in culture. The ferroquine mean IC50 (95% CI) was 9.3 nM (95% C.I.: 8.7 – 10.0). The mean IC50 value for the principal metabolite of ferroquin, SR97213A, was 37.0 nM (95% C.I.: 34.3 – 39.9), which is four times less active than ferroquine. The isolates in this study were highly multi-drug resistant but ferroquine was more active than chloroquine, quinine, mefloquine and piperaquine. Only artesunate was more active than ferroquine. Weak but significant correlations were found between ferroquine and its principal metabolite (r2 = 0.4288), chloroquine (r2 = 0.1107) and lumefantrine (r2 = 0.2364).

Conclusion

The results presented in this study demonstrate that the new ferroquine compound SSR97193 has high anti-malarial activity in vitro against multi-drug resistant P. falciparum.

Background

The emergence of drug resistant malaria is a serious threat for malarial control [1, 2]. Currently, chloroquine-resistant Plasmodium falciparum parasites are prevalent in most of the tropics, and in many areas resistance is high grade (i.e. potentially dangerous, with early treatment failures occurring) [1, 2]. The rapid spread of chloroquine resistance has forced clinicians in many regions of the world to abandon classical therapy with chloroquine, in favour of other drugs that are less well tolerated and, importantly, more expensive. The situation in South-east Asia is of particular concern with increasingly frequent cases of 'multi-drug' resistant malaria [3]. On the borders of Thailand, P. falciparum has become resistant to nearly all available drugs [4, 5]. These observations highlight the urgent need to find new antimalarial drugs or drug combinations and to develop optimal treatment protocols.

Most new drugs arise from the identification of new therapeutic targets or metabolic pathways. Another approach is to modify an existing drug to enhance its activity [6]. Ferroquine (SSR97193) is a new 4-aminoquinoline antimalarial active against chloroquine resistant and sensitive P falciparum strains in vivo and in vitro [68] (Figure 1). This antimalarial organic iron complex (a ferrocenyl group has been associated with chloroquine) is meant to use the affinity of Plasmodium for iron to increase the probability for encountering the anti-malarial molecule [69]. The ferrocene group by itself does not have antimalarial activity [8].
Figure 1

Chemical structure of ferroquine (7-chloro-4-[(2-N,N-dimethyl-aminomethyl) ferrocenylmethylamino]quinoline) and its principal metabolite.

Four previous studies had investigated the activity of ferroquine against P. falciparum isolates from infected patients [1013] (Table 1). However, the drug sensitivity of P. falciparum strains varies between different locations, where P. falciparum isolates have different anti-malarial resistance backgrounds.
Table 1

Publications on the activity of ferroquine against P. falciparum isolates

     

Drug IC50 (Geometric mean)

 
 

Year

Country

Region

No. of isolates

Ferroquine

Chloroquine

Method

Pradine et al. [10]

2001

Gabon

Libreville

103

10.8 nM

370 nM

Isotopic microtest

Atteke et al. [12]

2003

Gabon

Franceville

56

16.0 nM

141.3 nM

Isotopic microtest

   

Bakoumba

60

27.9 nM

398.0 nM

 

Pradine et al. [11]

2002

Senegal

 

55

7.9 nM

102 mM

Isotopic microtest

Chim et al. [13]

2004

Cambodia

 

127

30.61 nM

125.04 nM

Isotopic microtest

The aim of the present study was to confirm the efficiency of ferroquine against P. falciparum isolates from patients treated for malaria in one of the SMRU clinics along the Thai-Burmese border. This area has a known high multi-drug-resistance rate, with very high resistance against chloroquine [14].

Methods

Isolates of Plasmodium falciparum

Between November 2004 and May 2005 parasite isolates were obtained from patients with acute falciparum malaria attending the clinics of the Shoklo Malaria Research Unit (SMRU). The clinics are open for both migrants and refugees and are situated in an area of forested hills on the northwestern border of Thailand. Isolates were collected if the parasitaemia was at least 0.1%, by taking 5 cc whole blood by venopunture into sterile Vacutainer® tubes containing 0.05 ml K3 EDTA. The blood samples were transported within 4–6 hours at room temperature and then set up in continuous culture upon their arrival in the SMRU laboratory in Mae Sot, not more than one-hour drive from the study sites. Samples were only provided by patients who gave written, informed consent following written and oral explanations given in their own language. This study was part of a series of treatment trials approved by the Ethical Committee of the Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

In vitro drug assay

Ferroquine (FQ) (SSR97193: 7-chloro-4-[(2-N,N-dimethyl-aminomethyl) ferrocenylmethylamino]quinoline), and ferroquine metabolite (FQM) (SR97213A: C22H22ClFeN3) (Figure 1) were obtained from Sanofi-Synthelabo-Recherche, France. Chloroquine diphosphate (CQ), quinine citrate (QN) and doxycyline hydrochloride (DOX) were obtained from Sigma Chemicals, UK. Lumefantrine (LUM) was obtained from Novartis Pharmacia, Basel, Switzerland. Sodium artesunate (AS), piperaquine (PIP) and mefloquine (MFQ) were kindly donated by Dr. Niklas Lindegård, Faculty of Tropical Medicine, Mahidol University, Thailand. FQ and FQM were dissolved in DMSO, QN, MFQ and AS in 70% ethanol, CQ and DOX in deionised water, PIP in 100% methanol and LUM was dissolved in a 1:1:1 (w/v) mixture of ethanol, Triton-X (Sigma), linoleic acid (Sigma). All drugs were dissolved initially at a concentration of 1 mg/ml, and serial dilutions were made in complete RPMI medium. The solvent in the final concentrations had no significant effect on parasite growth when compared to culture media. All concentrations, including drug-free controls, were distributed in duplicate in 96-well tissue culture plates. The drug-plates were made in bulk and stored at -80°C until use (for up to three months).

For each sample, plasma and buffy coat were removed after centrifugation and the red cells washed three times in phosphate buffered saline (PBS). The infected red blood cells were set-up in the pre-dosed drug plates in complete RPMI with 10% heterologous sera, at a parasitaemia of 0.1% parasitized erythrocytes and a haematocrit of 1.5%. The plates were incubated at 37°C in the presence of 5% CO2, 90% N2 and 5% O2 for 42 hours. After culture the plates were frozen down at -20°C.

The chloroquine-resistant P. falciparum laboratory clone K1 was used for quality control of the drug-plates.

DELI

The double-site enzyme-linked pLDH immunodetection (DELI) assay was used to assess P. falciparum antimalarial drug susceptibility. The DELI method was performed as described previously [15, 16]. In brief, the culture plates were thawed and frozen three times order to lyse the cells. 100 μl from each well were transferred into 96-well plates (Nunc-Immuno™ plate, maxisorb, Nalgene Nunc International, Denmark) pre-coated with a capture monoclonal antibody 17E4, which specifically recognizes the pLDH, incubated for 1 hour at 37°C. Following washing 3× with PBS/0.5% bovine serum albumin (BSA Fraction V) (Roche Diagnostics, Mannheim, Germany), a second biotinylated anti-pLDH monoclonal antibody 19G7 was added and the plates incubated for 1 hour at 37°C. After removal of unbound antibody by washing 3× with PBS/0.5% BSA, the plates were incubated at room temperature for 30 minutes with a 1:10,000 solution of streptavidin-POD conjugate (Roche Diagnostics). After washing the plates 3× with PBS/0.5% BSA, the plates were incubated for up to 20 minutes at room temperature with a peroxidase substrate solution, 3,3',5,5'-tetramethylbenzidine (KPL, Maryland, USA). The reaction was stopped with 1 M phosphoric acid and colour development was quantified immediately using a spectrophotometer to determine the OD at 450 nm with a reference filter at 690 nm.

Analysis of dose response curves

Dose response curves, IC50 values, and coefficients of variation were calculated by fitting the data to an inhibitory E-max pharmacokinetic model using WINNONLIN Ver 4.1 (Pharsight Corporation). To ensure data quality we rejected all IC50 values with coefficients of variation (Standard Error × 100)/Mean) of estimated IC50 values > 30% and those in which the pLDH production in control wells (parasites, no drug) was < 5 times background (red blood cells only).

Statistical analysis

Data were analysed using the program SPSS 11.0 for Windows (SPSS Inc., Chicago, Illinois, USA). Prior to analysis, in vitro drug response data were normalized by log transformation. Ferroquine cross-resistance with the other antimalarials (metabolite, chloroquine, artesunate, quinine, mefloquine, lumefantrine, doxycyclin and piperaquine) was estimated by Pearson correlation coefficient (r) and coefficient of determination (r2).

Results

Sixty-five P. falciparum isolates from non-pregnant patients were successfully grown in culture and gave interpretable results for the calculation of the mean drug IC50 values as shown in Table 2. The ferroquine mean IC50 was 9.30 (95% C.I.: 8.69 – 9.96) nM, with individual values ranging from 3.86 to 18.23 nM. The mean IC50 value for ferroquine metabolite, the principal metabolite of ferroquine, was 37.00 nM (95% C.I.: 34.32 – 39.89), which is almost 4 times less active than ferroquine. All isolates were resistant against chloroquine using the standard cut-off IC50 of 100 nM. The mean IC50 value for chloroquine was 340.75 nM (95% C.I.: 304.04 – 381.89), which makes ferroquine 36-fold more potent than chloroquine in these isolates. A weak but significant correlation was found between ferroquine with ferroquine metabolite (r = 0.655, r2 = 0.4288, P = 0.0001) and ferroquine with chloroquine (r = 0.333, r2 = 0.1107, P = 0.009 (Table 2). Also a significant correlation was observed between the IC50 for ferroquine and quinine. However this correlation was not reproducible in a second set of samples. No significant correlation between ferroquine and artesunate was detected.
Table 2

The in vitro IC50 responses of the 65 isolates of Plasmodium falciparum to ferroquine, ferroquine metabolite, chloroquine, artesunate and quinine. Ferroquine cross-resistance with the other antimalarials was estimated by Pearson correlation coefficient (r), and coefficient of determination (r2)

  

Mean inhibitory concentration (nM)

   

Drug

No. of isolates

Geometric mean

95% confidence interval

r

r 2

P

Ferroquine

65

9.30

8.69 – 9.96

-

-

-

FQ-Metabolite

64

37.00

34.32 – 39.89

0.655

0.4288

0.0001

Chloroquine

62

340.75

304.04 – 381.89

0.333

0.1107

0.0090

Artesunate

56

4.02

3.06 – 6.28

-0.169

0.0284

0.2190

Quinine

49

1016.05

894.36 – 1154.29

0.338

0.1145

0.0200

For a subset of the P. falciparum isolates (n = 22) the IC50 values for doxycycline, lumefantrine, mefloquine and piperaquine were analysed as well (Table 3). These data showed that ferroquine was more active than any of these drugs in this group of isolates. Only for lumefantrine a significant correlation with ferroquine was found (r = 0.486, r2 = 0.2364, P = 0.025).
Table 3

The in vitro IC50 responses of a subset of Plasmodium falciparum isolates (n = 22) to Ferroquine, Ferroquine metabolite, Doxycyxline, Lumefantrine, Mefloquine, and Piperaquine. Ferroquine cross-resistance with the other antimalarials was estimated by Pearson correlation coefficient (r), and coefficient of determination (r2).

  

Mean inhibitory concentration (nM)

   

Drug

No. of isolates

Geometric mean

95% confidence interval

r

r 2

P

Ferroquine

21

8.91

8.17 – 9.72

-

-

-

FQ-Metabolite

22

31.40

28.16 – 35.01

0.674

0.4542

0.001

Doxycycline

20

2719.85

2236.38 – 3307.09

-0.203

0.0411

0.391

Lumefantrine

22

11.77

10.31 – 13.44

0.486

0.2364

0.025

Mefloquine

21

144.23

125.82 – 165.33

0.296

0.0874

0.206

Piperaquine

19

49.20

37.16 – 65.11

0.017

0.0003

0.946

Discussion

This study confirms that ferroquine (SSR97193) is highly active against P. falciparum in vitro. The P. falciparum isolates analysed in this study were highly multi-drug resistant but ferroquine was more active than chloroquine, quinine, mefloquine, piperaquine and doxycycline. Only artesunate was more active than ferroquine. In addition, ferroquine was four times more active in vitro than the tested principal ferroquine metabolite (SR97213A). This underlines the importance to test the biological activity of the plasma and whole blood of treated patients or volunteers, as other metabolites may be active.

Weak but significant correlations between the response to ferroquine and that to chloroquine and lumefantrine were found. The high activity of ferroquine on chloroquine-resistant P. falciparum (the lowest CQ IC50 analysed is 102.4 nM) suggests either that both drugs have different modes of action, or that ferroquine reverses chloroquine resistance [12]. Chloroquine is believed to act by concentrating in the parasite digestive vacuole and preventing the conversion of toxic heme to haemozoin, leading to membrane damage and parasite death [17, 18]. Biot et. al. recently demonstrated that ferroquine, like chloroquine, forms complexes with haematin and is an even stronger inhibitor of β-haematin formation than chloroquine [9]. Chloroquine-resistant parasites expel chloroquine much more rapidly from red blood cells than chloroquine-sensitive parasites. This efflux of chloroquine is catalyzed by a P. falciparum transmembrane protein (Pf CRT) [18]. Ferroquine may block the Pf CRT through its lipophilic properties, acting like a resistance-reversing agent [9].

So far, no resistance of P. falciparum to ferroquine has been found in vitro either in cultures of patient isolates or in laboratory-adapted strains under drug pressure [18].

In Table 1, the published studies on the in vitro susceptibility to ferroquine and chloroquine are summarized. The isolates tested in the present study show comparable in vitro sensitivity to ferroquine to that found in Gabon (Libreville) [10] and Senegal [11]. Isolates from Cambodia have the highest IC50 values for ferroquine. However, caution must be exerted when comparing these results because in the present study a DELI method rather than an isotopic assay is used. Previous reports have demonstrated that DELI does slightly overestimate the IC50 for chloroquine and lumefantrine, and underestimate for artesunate, compared to the isotopic microtest [14]. No data on the direct comparison of DELI with the isotopic microtest for ferroquine levels are available as yet.

The results presented in this study indicate that ferroquine is active in vitro regardless of high grade multi-drug resistance. Still further research is needed to elucidate the mode of action of ferroquine and identify the putative molecular markers of resistance. In addition, since chloroquine resistance is also found in Plasmodium vivax [19], the activity of ferroquine to P. vivax should be studied.

Declarations

Acknowledgements

We would like to thank all the patients and staff of the Shoklo Malaria Research Unit. We are also grateful to Sanofi-Aventis and Dr. Niklas Lindegårdh for providing the drugs used in the in vitro assays. This study received financial support from Sanofi-Aventis. The Shoklo Malaria Research Unit is part of the Wellcome Trust-Mahidol University, Oxford Tropical Medicine Research Programme sponsored by The Wellcome Trust of Great Britain.

Authors’ Affiliations

(1)
Shoklo Malaria Research Unit
(2)
Faculty of Tropical Medicine, Mahidol University
(3)
Centre for Vaccinology and Tropical Medicine, Churchill Hospital, Oxford

References

  1. White NJ: Antimalarial drug resistance and combination chemotherapy. Philos Trans R Soc Lond B Biol Sci. 1999, 354: 739-749. 10.1098/rstb.1999.0426.PubMed CentralView ArticlePubMedGoogle Scholar
  2. Barnes KI, White NJ: Population biology and antimalarial resistance: the transmission of antimalarial drug resistance in Plasmodium falciparum. Acta Trop. 2005, 94: 230-240.View ArticlePubMedGoogle Scholar
  3. World Health Organization: World malaria report 2005. 2005, GenevaView ArticleGoogle Scholar
  4. Reacher M, Campbell CC, Freeman J, Dobersteyn EB, Brandling-Bennet AD: Drug therapy for Plasmodium falciparum malaria resistant to pyrimethamine-sulfadoxine (Fansidar). A study of alternate regimens in Eastern Thailand, 1980. Lancet. 1981, 2: 1066-1069. 10.1016/S0140-6736(81)91274-5.View ArticlePubMedGoogle Scholar
  5. White NJ: Antimalarial drug resistance: the pace quickens. J Antimicrob Chemother. 1992, 30: 571-585. 10.1093/jac/30.5.571.View ArticlePubMedGoogle Scholar
  6. Delhaes L, Abessolo H, Biot C, Berry L, Delcourt P, Maciejewski L, Brocard J, Camus D, Dive D: In vitro and in vivo antimalarial activity of ferrochloroquine, a ferrocenyl analogue of chloroquine against chloroquine-resistant malaria parasites. Parasitol Res. 2001, 87: 239-244. 10.1007/s004360000317.View ArticlePubMedGoogle Scholar
  7. Biot C, Glorian G, Maciejewski L, Brocard J, Domarle O, Blampain G, Millet P, Georges AJ, Abessolo H, Dive D, Lebibi J: Synthesis and antimalarial activity in vitro and in vivo of a new ferrocene-chloroquine analogue. J Med Chem. 1997, 40: 3715-3718. 10.1021/jm970401y.View ArticlePubMedGoogle Scholar
  8. Domarle O, Blampain G, Agnaniet H, Nzadiyabi T, Lebibi J, Brocard J, Maciejewski L, Biot C, Georges AJ, Millet P: In vitro antimalarial activity of a new organometallic analog, ferrocene-chloroquine. Antimicrob agents Chemother. 1998, 42 (3): 540-544.PubMed CentralPubMedGoogle Scholar
  9. Biot C, Taramelli D, Forfar-Bares I, Maciejewski L, Boyce M, Nowogrocki G, Brocard J, Basilico N, Olliaro P, Egan TJ: Insights into the mechansims of action of ferroquine. Relationship between physicochemical properties and antiplasmodial activity. Mol Pharm. 2005, 2: 185-193. 10.1021/mp0500061.View ArticlePubMedGoogle Scholar
  10. Pradines B, Fusai T, Daries W, Laloge V, Rogier C, Millet P, Panconi E, Kombila M, Parzy D: Derrocene-chloroquine analogues as antimalarial agents: in vitro activity of ferrochloroquine against 103 Gabonese isolates of Plasmodium falciparum. J Antimicrob Chemother. 2001, 48: 179-184. 10.1093/jac/48.2.179.View ArticlePubMedGoogle Scholar
  11. Pradines B, Tall A, Rogier C, Spiegel A, Mosnier J, Marrama L, Fusai T, Millet P, Panconi E, Trape JF, Parzy D: In vitro activities of ferrochloroquine against 55 Senegalese isolates of Plasmodium falciparum in comparison with those of standard antimalarial drugs. Trop Med Int Health. 2002, 7: 265-270. 10.1046/j.1365-3156.2002.00848.x.View ArticlePubMedGoogle Scholar
  12. Atteke C, Ndong JN, Aubouy A, Maciejewski L, Brocard J, Lebibi J, Deloron P: In vitro susceptibility to a new antimalarial organometallic analogue, ferroquine, of Plasmodium falciparum isolates from the Haut-Ogooue region of Gabon. J Antimicrob Chemother. 2003, 51: 1021-1024. 10.1093/jac/dkg161.View ArticlePubMedGoogle Scholar
  13. Chim P, Lim P, Sem R, Nhem S, Maciejewski L, Fandeur T: The in-vitro antimalarial activity of ferrochloroquine measured against Cambodian isolates of Plasmodium falciparum. Ann Trop Med Parasitol. 2004, 98: 419-424. 10.1179/000349804225003361.View ArticlePubMedGoogle Scholar
  14. Brockman A, Price RN, van Vugt M, Heppner DG, Walsh D, Sookto P, Wimonwattrawatee T, Looareesuwan S, White NJ, Nosten F: Plasmodium falciparum antimalarial drug susceptibility on the north-western border of Thailand during five years of extensive use of artesunate-mefloquine. Trans R Soc Trop Med Hyg. 2000, 94: 537-544. 10.1016/S0035-9203(00)90080-4.PubMed CentralView ArticlePubMedGoogle Scholar
  15. Druilhe P, Moreno A, Blanc C, Brasseur PH, Jacquier P: A colorimetric in vitro sensitivity assay for Plasmodium falciparum based on a highly sensitive double-site LDH antigen capture ELISA. Am J Trop Med Hyg. 2001, 64: 233-241.PubMedGoogle Scholar
  16. Brockman A, Singlam S, Phiaphun L, Looareesuwan S, White NJ, Nosten F: Field evaluatiuon of a novel colorimetric method – double-site enzym-linked lactate dehydrogenase immunodetection assay – to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand. Antimicrob agents Chemother. 2004, 48: 1426-1429. 10.1128/AAC.48.4.1426-1429.2004.PubMed CentralView ArticlePubMedGoogle Scholar
  17. Dorn A, Vippagunta SR, Matile H, Jaquet C, Vennerstrom JL, Ridley RG: An assesment of drug-haematin binding as a mechanism for inhibition of haematin polymerisation by quinoline antimalarials. Biochem Pharmacol. 1998, 55: 727-736. 10.1016/S0006-2952(97)00510-8.View ArticlePubMedGoogle Scholar
  18. Daher W, Biot C, Fandeur T, Jouin H, Pelinski L, Viscogliosi E, Fraisse L, Pradines B, Brocard J, Khalife J, Dive D: Assessment of Plasmodium falciparum resistance to ferroquine (SSR97193) in field isolates and in W2 strain under pressure. Malaria Journal. 2006, 5: 11-10.1186/1475-2875-5-11.PubMed CentralView ArticlePubMedGoogle Scholar
  19. Maguire JD, Krisin , Marwoto H, Richie TL, Fryauff DJ, Baird JK: Mefloquine is highly efficacious against chloroquine-resistant Plasmoidum vivax malaria and Plasmodium falciparum malaria in Papua, Indonesia. Clin Infect Dis. 2006, 42: 1073-1074. 10.1086/501357.View ArticleGoogle Scholar

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